果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通...果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通过分析果实不同时期不同部位的转录组表达差异。结果表明,编码花青素合成的Achn385311(3GGT)基因可能是控制‘红实2号’内果皮呈红色的主效基因,‘金实1号’后期果肉呈深黄色可能与Achn158981(GT7)、Achn150731、Achn068721(PAO)、Achn282201(PAO)和Achn176251(UGT71A16)等基因的表达调控有关。针对差异表达的230个猕猴桃果肉颜色相关基因,利用Primer Premier 5.0设计出727对EST-SSR引物,以7份不同果肉颜色的猕猴桃基因组DNA为模板,随机选择112对引物进行有效性验证,筛选出了具有清晰的扩增产物的引物78对,有效扩增率为69.64%,58对具有多态性,多态性频率为74.36%。本研究结果为猕猴桃果实呈色机理研究及分子标记辅助育种提供了理论基础。展开更多
为探究栽培型古茶树阿萨姆茶(Camellia sinensis var. assamica)种质资源的遗传多样性,采用EST-SSR分子标记技术对云南南涧县无量山镇古茶园64份种质资源进行遗传多样性和遗传结构分析。结果表明, 20对引物共检测出223个等位基因,群体...为探究栽培型古茶树阿萨姆茶(Camellia sinensis var. assamica)种质资源的遗传多样性,采用EST-SSR分子标记技术对云南南涧县无量山镇古茶园64份种质资源进行遗传多样性和遗传结构分析。结果表明, 20对引物共检测出223个等位基因,群体间平均有效等位基因数为3.48个;观测等位基因数(N_(a))为6.25;有效等位基因数(N_(e))为2.983;Shannon多样性指数(I)为1.251;Nei基因多样性指数(H)为0.646。POPGENE分析表明遗传分化系数(F_(st))为0.063,居群间存在中度分化,基因流(N_(m))为3.710。AMOVA分子方差分析表明,阿萨姆茶的遗传变异14%发生在居群间,86%发生在居群内,说明阿萨姆茶居群遗传变异主要发生在居群内部,且基因交流丰富。南涧县古茶园古茶树居群的遗传多样性丰富,这为阿萨姆茶种质资源的保护利用和新品种选育提供了科学依据。展开更多
When a steel plate produced by a steel plant leaves the factory,its upper surface should be marked with the specification,variety,batch number,and other identifying information,and its side should be marked with a sid...When a steel plate produced by a steel plant leaves the factory,its upper surface should be marked with the specification,variety,batch number,and other identifying information,and its side should be marked with a side marking.The side marking contains the plate number,specification,batch number,and other information,which are convenient for customers to accurately identify relevant information after stacking.However,because of the influence of weather,environment,and other factors,the side marking becomes unidentifiable when the steel plate is corroded,affecting the customer’s loading,unloading,and other operations.The development and application of side anticorrosion devices and cleaning of finished heavy steel plates with antirust agents will solve the problem of unrecognizable side marking due to corrosion.展开更多
Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different...Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl_(2)·6 H_(2)O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.展开更多
Accurate estimations of animal population size are pivotal for implementing management strategies properly.Recapture technique based on sounds as a specimen identifcation mark has barely been used for marine mammals.H...Accurate estimations of animal population size are pivotal for implementing management strategies properly.Recapture technique based on sounds as a specimen identifcation mark has barely been used for marine mammals.However,inferring abundance estimates from acoustic methods could enhance the accuracy and precision of population size assessments.Here,we tested the possibility of using signature whistles as individual marks for estimating the size of common bottlenose dolphin(Tursiops truncatus)populations.Data were continuously collected for 326 days in 2015–2016,by using a fxed acoustic device located in the Sicily Strait(Italy).The SIGID method was applied to identify Signature Whistles Types(SWTs)over 7,000 h of recordings.Eighty SWTs were detected as long as their stereotyped fundamental frequency contours were repeated in bouts of at least 6 renditions.The mean SWTs monthly recording rate resulted in 0.19(Standard deviation=0.16),with 20 SWTs recorded over 5 or more different encounters(until a maximum of 30 encounters).The Jolly–Seber model(with POPAN formulation)was run in Mark software to estimate the population size.The estimated population size resulted in 171 bottlenose dolphins(95%confdence interval=137–215).Even if the detection and identifcation of signature whistles required crucial precautions,and animals could be detected differently from visual techniques,the population size estimate obtained was comparable with previous results based on physical marks data.These outcomes demonstrated that signature whistles can be considered a strongly effective tool for integrating traditional mark-recapture techniques with fnely estimated dolphins’population abundances.展开更多
AIM:To compare the accuracy of manual marking versus an image-guided system for toric implantable collamer lens(TICL)implantation and evaluate the short-term postoperative rotational stability of TICL and corneal surg...AIM:To compare the accuracy of manual marking versus an image-guided system for toric implantable collamer lens(TICL)implantation and evaluate the short-term postoperative rotational stability of TICL and corneal surgically induced astigmatism vector(SIA).METHODS:Retrospective analysis was conducted on eyes with TICL alignment achieved through manual marking(n=75)or VERION image-guided system-assisted marking(n=83).Each group was further classified into horizontal and vertical subgroups based on implant orientation.Additionally,patients were categorized into superior and temporal incision subgroups according to the position of main corneal incision.The misalignment and rotational stability of TICL were analyzed using slit-lamp anterior segment photography.Surgical predictability,efficacy,safety,and corneal SIA were also evaluated.RESULTS:In general,the TICL implantation with manual and digital image-guided systems all achieved robust predictability,efficacy,and safety.The misalignment of TICL was comparable between the manual and VERION groups(0.16°±3.97°vs 0.52°±5.59°,P=0.633),while a significant difference was observed in the absolute misalignment of TICL between the two groups(3.02°±2.55°vs 4.28°±3.61°,P=0.043).There were no significant differences in the distribution of TICL misalignment between the manual and VERION groups or between horizontal and vertical implant orientation groups(P>0.05).Furthermore,different orientations of TICL placement did not show statistically significant differences in rotational stability(P=0.46).Statistically significant differences were found in anterior corneal SIA between the manual and VERION groups(0.46±0.27 vs 0.33±0.21 D,P=0.001),especially for superior incision position(0.60±0.27 vs 0.35±0.23 D,P<0.0001).The anterior SIA exhibited a significant difference between superior and temporal incisions in the manual group(0.60±0.27 vs 0.35±0.20 D,P<0.0001).CONCLUSION:Compared with the conventional manual marking method,this study indicates that the digital image-guided system with VERION is safe and effective in TICL implantation.The digital system offers the advantage of minimizing corneal SIA compared to the manual method.展开更多
文摘果肉颜色是猕猴桃重要的品质性状,不同果肉颜色的猕猴桃在色素成分、糖和维生素C含量等方面存在很大差异。为了探究猕猴桃果肉颜色形成的分子机制,本研究以红肉猕猴桃‘红实2号’和黄肉猕猴桃‘金实1号’为研究对象,采用RNA-seq技术,通过分析果实不同时期不同部位的转录组表达差异。结果表明,编码花青素合成的Achn385311(3GGT)基因可能是控制‘红实2号’内果皮呈红色的主效基因,‘金实1号’后期果肉呈深黄色可能与Achn158981(GT7)、Achn150731、Achn068721(PAO)、Achn282201(PAO)和Achn176251(UGT71A16)等基因的表达调控有关。针对差异表达的230个猕猴桃果肉颜色相关基因,利用Primer Premier 5.0设计出727对EST-SSR引物,以7份不同果肉颜色的猕猴桃基因组DNA为模板,随机选择112对引物进行有效性验证,筛选出了具有清晰的扩增产物的引物78对,有效扩增率为69.64%,58对具有多态性,多态性频率为74.36%。本研究结果为猕猴桃果实呈色机理研究及分子标记辅助育种提供了理论基础。
文摘为探究栽培型古茶树阿萨姆茶(Camellia sinensis var. assamica)种质资源的遗传多样性,采用EST-SSR分子标记技术对云南南涧县无量山镇古茶园64份种质资源进行遗传多样性和遗传结构分析。结果表明, 20对引物共检测出223个等位基因,群体间平均有效等位基因数为3.48个;观测等位基因数(N_(a))为6.25;有效等位基因数(N_(e))为2.983;Shannon多样性指数(I)为1.251;Nei基因多样性指数(H)为0.646。POPGENE分析表明遗传分化系数(F_(st))为0.063,居群间存在中度分化,基因流(N_(m))为3.710。AMOVA分子方差分析表明,阿萨姆茶的遗传变异14%发生在居群间,86%发生在居群内,说明阿萨姆茶居群遗传变异主要发生在居群内部,且基因交流丰富。南涧县古茶园古茶树居群的遗传多样性丰富,这为阿萨姆茶种质资源的保护利用和新品种选育提供了科学依据。
文摘When a steel plate produced by a steel plant leaves the factory,its upper surface should be marked with the specification,variety,batch number,and other identifying information,and its side should be marked with a side marking.The side marking contains the plate number,specification,batch number,and other information,which are convenient for customers to accurately identify relevant information after stacking.However,because of the influence of weather,environment,and other factors,the side marking becomes unidentifiable when the steel plate is corroded,affecting the customer’s loading,unloading,and other operations.The development and application of side anticorrosion devices and cleaning of finished heavy steel plates with antirust agents will solve the problem of unrecognizable side marking due to corrosion.
基金Supported by the National Key Research and Development Program of China(No.2022 YFF 0608203)the Graduate Student Scientific Research Innovation Projects in Jiangsu Province(No.KYCX 22-0706)。
文摘Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl_(2)·6 H_(2)O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.
基金the project“BIOforIU project PONa3_00025-Multidisciplinary Infrastructure for the Study and Development of Marine and Terrestrial Biodiversity in the Perspective of Innovation Union.”Part of the analysis was funded under the National Recovery and Resilience Plan(NRRP),Mission 4 Component 2 Investment 1.4-Call for tender No.3138 of 16 December 2021,rectifed by Decree n.3175 of 18 December 2021 of Italian Ministry of University and Research funded by the European Union-NextGenerationEU,Award Number:Project code CN_00000033,Concession Decree No.1034 of 17 June 2022 adopted by the Italian Ministry of University and Research,Project title"National Biodiversity Future Center-NBFC".
文摘Accurate estimations of animal population size are pivotal for implementing management strategies properly.Recapture technique based on sounds as a specimen identifcation mark has barely been used for marine mammals.However,inferring abundance estimates from acoustic methods could enhance the accuracy and precision of population size assessments.Here,we tested the possibility of using signature whistles as individual marks for estimating the size of common bottlenose dolphin(Tursiops truncatus)populations.Data were continuously collected for 326 days in 2015–2016,by using a fxed acoustic device located in the Sicily Strait(Italy).The SIGID method was applied to identify Signature Whistles Types(SWTs)over 7,000 h of recordings.Eighty SWTs were detected as long as their stereotyped fundamental frequency contours were repeated in bouts of at least 6 renditions.The mean SWTs monthly recording rate resulted in 0.19(Standard deviation=0.16),with 20 SWTs recorded over 5 or more different encounters(until a maximum of 30 encounters).The Jolly–Seber model(with POPAN formulation)was run in Mark software to estimate the population size.The estimated population size resulted in 171 bottlenose dolphins(95%confdence interval=137–215).Even if the detection and identifcation of signature whistles required crucial precautions,and animals could be detected differently from visual techniques,the population size estimate obtained was comparable with previous results based on physical marks data.These outcomes demonstrated that signature whistles can be considered a strongly effective tool for integrating traditional mark-recapture techniques with fnely estimated dolphins’population abundances.
文摘AIM:To compare the accuracy of manual marking versus an image-guided system for toric implantable collamer lens(TICL)implantation and evaluate the short-term postoperative rotational stability of TICL and corneal surgically induced astigmatism vector(SIA).METHODS:Retrospective analysis was conducted on eyes with TICL alignment achieved through manual marking(n=75)or VERION image-guided system-assisted marking(n=83).Each group was further classified into horizontal and vertical subgroups based on implant orientation.Additionally,patients were categorized into superior and temporal incision subgroups according to the position of main corneal incision.The misalignment and rotational stability of TICL were analyzed using slit-lamp anterior segment photography.Surgical predictability,efficacy,safety,and corneal SIA were also evaluated.RESULTS:In general,the TICL implantation with manual and digital image-guided systems all achieved robust predictability,efficacy,and safety.The misalignment of TICL was comparable between the manual and VERION groups(0.16°±3.97°vs 0.52°±5.59°,P=0.633),while a significant difference was observed in the absolute misalignment of TICL between the two groups(3.02°±2.55°vs 4.28°±3.61°,P=0.043).There were no significant differences in the distribution of TICL misalignment between the manual and VERION groups or between horizontal and vertical implant orientation groups(P>0.05).Furthermore,different orientations of TICL placement did not show statistically significant differences in rotational stability(P=0.46).Statistically significant differences were found in anterior corneal SIA between the manual and VERION groups(0.46±0.27 vs 0.33±0.21 D,P=0.001),especially for superior incision position(0.60±0.27 vs 0.35±0.23 D,P<0.0001).The anterior SIA exhibited a significant difference between superior and temporal incisions in the manual group(0.60±0.27 vs 0.35±0.20 D,P<0.0001).CONCLUSION:Compared with the conventional manual marking method,this study indicates that the digital image-guided system with VERION is safe and effective in TICL implantation.The digital system offers the advantage of minimizing corneal SIA compared to the manual method.
