Coated-PMMA microspheres consisting of poly （methyl methacrylate）（PMMA） core and barium titanate （BaTiO3） shell were synthesized by the modified sol-gel processing and then adopted as an electrorheological （ER...Coated-PMMA microspheres consisting of poly （methyl methacrylate）（PMMA） core and barium titanate （BaTiO3） shell were synthesized by the modified sol-gel processing and then adopted as an electrorheological （ER） materials. The structure and morphology of coated powders were chyfecterized by SEM and FT-IR; the shear stress of the suspensions of coated-PMMA particles and pure PMMA particles in silicone oil with a 20 vol% were investigated. The results show that the BaTiO3 coated PMMA microspheres based suspension in silicone oil exhibited typical ER behavior and stronger ER effects.展开更多
Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that pla...Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that plays an important role in a broad range of cellular activities from calcium regulation to protein synthesis and trafficking.In neurons,the ER extends from the soma through the axon to presynaptic terminals,and throughout the dendritic arbor into as many as half of all postsynaptic dendritic spines at any given time(Falahati et al.,2022).展开更多
Objective:To assess the antitumor activity of the novel chitinase produced by fermented,isolated Trichoderma viride in a hepatocellular carcinoma(HCC)male rat model.Methods:Diethyl-nitrosamine induction combined with ...Objective:To assess the antitumor activity of the novel chitinase produced by fermented,isolated Trichoderma viride in a hepatocellular carcinoma(HCC)male rat model.Methods:Diethyl-nitrosamine induction combined with ionizing radiation exposure was used to establish the HCC rat model.All rats were divided into 4 groups:the control group,the chitinase group,the HCC group,and the HCC+chitinase group.The antiproliferative effect of chitinase was evaluated in human HCC cells.The effect of chitinase in vivo on oxidative stress,endoplasmic reticulum stress chaperones,autophagy markers,PI3K/AKT/mTOR,AMPK pathway expression,and apoptotic indicators was determined and confirmed by histological examination.Results:Chitinase significantly inhibited the viabilities of HepG2 cells.Moreover,in the Wistar male rat model of HCC,chitinase decreased ATP levels,modulated endoplasmic reticulum stress,mediated autophagy factors,and promoted apoptosis.Conclusions:Chitinase might play a role in the apoptosis as well as autophagy pathways and may act as a potential tumor suppressor.展开更多
Objective:To investigate the efficacy and underlying mechanisms of standardized Salvia miltiorrhiza extract(SMEX)in alleviating menopausal symptoms using MCF-7 cells and an ovary-intact menopause mouse model resulting...Objective:To investigate the efficacy and underlying mechanisms of standardized Salvia miltiorrhiza extract(SMEX)in alleviating menopausal symptoms using MCF-7 cells and an ovary-intact menopause mouse model resulting from hypothalamic-pituitary-ovarian axis aging.Methods:Estrogen receptor(ER)-related molecular responses were first assessed in MCF-7 cells treated with SMEX.In vivo efficacy was then evaluated in 52-week-old female mice orally administered SMEX(50 or 100 mg/kg/day)or 17β-estradiol(E2)for 12 weeks.ER expression and downstream AKT/ERK signaling pathways in uterine tissues were determined.In addition,histological analysis of reproductive organs,assessment of serum lipid and hormone levels,neurotransmitter measurements,and behavioral tests were performed.Results:SMEX upregulated ERαand ERβexpression and suppressed pS2 mRNA in MCF-7 cells,indicating selective ER modulation.In SMEX-treated mice,uterine ER expression and activation of the AKT and ERK pathways were significantly increased,leading to partial restoration of epithelial thickness and stratification in the oviduct and vagina.SMEX also significantly reduced serum low-density lipoprotein cholesterol levels and reversed menopausal alterations in the follicle-stimulating hormone/luteinizing hormone ratio.Additionally,it elevated serotonin and norepinephrine levels in the pituitary,thereby alleviating depression-like behavior.Conclusions:SMEX modulates ER signaling and improves neurohormonal balance,effectively alleviating menopausal symptoms in both in vitro and in vivo models.This highlights its potential as a safe,natural alternative to hormone replacement therapy and as a promising functional ingredient in therapeutic natural products.展开更多
基金Funded by the National Natural Science Foundation of China (Nos. 10418014 and 10474074).
文摘Coated-PMMA microspheres consisting of poly （methyl methacrylate）（PMMA） core and barium titanate （BaTiO3） shell were synthesized by the modified sol-gel processing and then adopted as an electrorheological （ER） materials. The structure and morphology of coated powders were chyfecterized by SEM and FT-IR; the shear stress of the suspensions of coated-PMMA particles and pure PMMA particles in silicone oil with a 20 vol% were investigated. The results show that the BaTiO3 coated PMMA microspheres based suspension in silicone oil exhibited typical ER behavior and stronger ER effects.
基金supported by AHA Career Development Award 938683 (to PJD)NIH grant R01MH123700 (to MLD)
文摘Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that plays an important role in a broad range of cellular activities from calcium regulation to protein synthesis and trafficking.In neurons,the ER extends from the soma through the axon to presynaptic terminals,and throughout the dendritic arbor into as many as half of all postsynaptic dendritic spines at any given time(Falahati et al.,2022).
文摘Objective:To assess the antitumor activity of the novel chitinase produced by fermented,isolated Trichoderma viride in a hepatocellular carcinoma(HCC)male rat model.Methods:Diethyl-nitrosamine induction combined with ionizing radiation exposure was used to establish the HCC rat model.All rats were divided into 4 groups:the control group,the chitinase group,the HCC group,and the HCC+chitinase group.The antiproliferative effect of chitinase was evaluated in human HCC cells.The effect of chitinase in vivo on oxidative stress,endoplasmic reticulum stress chaperones,autophagy markers,PI3K/AKT/mTOR,AMPK pathway expression,and apoptotic indicators was determined and confirmed by histological examination.Results:Chitinase significantly inhibited the viabilities of HepG2 cells.Moreover,in the Wistar male rat model of HCC,chitinase decreased ATP levels,modulated endoplasmic reticulum stress,mediated autophagy factors,and promoted apoptosis.Conclusions:Chitinase might play a role in the apoptosis as well as autophagy pathways and may act as a potential tumor suppressor.
基金funded by UNDBIO Co.,Ltd.No specific grant number was assigned.
文摘Objective:To investigate the efficacy and underlying mechanisms of standardized Salvia miltiorrhiza extract(SMEX)in alleviating menopausal symptoms using MCF-7 cells and an ovary-intact menopause mouse model resulting from hypothalamic-pituitary-ovarian axis aging.Methods:Estrogen receptor(ER)-related molecular responses were first assessed in MCF-7 cells treated with SMEX.In vivo efficacy was then evaluated in 52-week-old female mice orally administered SMEX(50 or 100 mg/kg/day)or 17β-estradiol(E2)for 12 weeks.ER expression and downstream AKT/ERK signaling pathways in uterine tissues were determined.In addition,histological analysis of reproductive organs,assessment of serum lipid and hormone levels,neurotransmitter measurements,and behavioral tests were performed.Results:SMEX upregulated ERαand ERβexpression and suppressed pS2 mRNA in MCF-7 cells,indicating selective ER modulation.In SMEX-treated mice,uterine ER expression and activation of the AKT and ERK pathways were significantly increased,leading to partial restoration of epithelial thickness and stratification in the oviduct and vagina.SMEX also significantly reduced serum low-density lipoprotein cholesterol levels and reversed menopausal alterations in the follicle-stimulating hormone/luteinizing hormone ratio.Additionally,it elevated serotonin and norepinephrine levels in the pituitary,thereby alleviating depression-like behavior.Conclusions:SMEX modulates ER signaling and improves neurohormonal balance,effectively alleviating menopausal symptoms in both in vitro and in vivo models.This highlights its potential as a safe,natural alternative to hormone replacement therapy and as a promising functional ingredient in therapeutic natural products.
