The evaluation of estrogenic activities and aryl hydrocarbon receptor (AhR) agonists in water from Three Gorges Reservoir (TGR) China was conducted by in vitro bioassays combined with SPMD-based virtual organisms (VO)...The evaluation of estrogenic activities and aryl hydrocarbon receptor (AhR) agonists in water from Three Gorges Reservoir (TGR) China was conducted by in vitro bioassays combined with SPMD-based virtual organisms (VO). VOs were deployed at seven sites in the Three Gorges Reservoir (TGR), China for two periods in 2009. The estrogenic activity was assessed using a rapid yeast estrogen bioassay, based on the expression of a green fluorescent reporter protein (yEGFP). The AhR activity was detected employing rat hepatoma cell line (H4IIE). The results indicate that AhR agonists distributed widely in water of TGR and almost homogenously distributed in most area of TGR. Weak antiestrogenic activities were also found homogenously distributed in water of TGR. Further studies are needed to determine the identities of these estrogenic compounds and AhR agonists and their potential adverse effects on wild biota in TGR.展开更多
We demonstrate a cw and actively Q-switched Er：LuAO laser resonantly dual-end-pumped by 1532nm fibre- coupled laser diodes. A maximum cw output power of 1.9W at 1650.3nm is obtained at a pump power of 25.5 W, corresp...We demonstrate a cw and actively Q-switched Er：LuAO laser resonantly dual-end-pumped by 1532nm fibre- coupled laser diodes. A maximum cw output power of 1.9W at 1650.3nm is obtained at a pump power of 25.5 W, corresponding to a slope efficiency of 43.3 %. In the Q-switched regime, the maximum pulse energy of 3.51 mJ is reached at a pulse repetition rate of 100 Hz, a pulse duration of 90.5ns and a pump power of 25.5 W. At the repetition rate of 400 Hz, the output energy is 2.12m J, corresponding to a pulse duration of 125.4 ns.展开更多
Estrogen Receptor-a （ERa） is the key transcription factor that regulates cell proliferation and homeosta- sis. In this pathway, estrogen plays an important role in genomic instability and cell cycle regulation pro- ...Estrogen Receptor-a （ERa） is the key transcription factor that regulates cell proliferation and homeosta- sis. In this pathway, estrogen plays an important role in genomic instability and cell cycle regulation pro- cesses and the mechanisms of its action are multifaceted. In this study, we showed that estrogen regulates genomic instability through promoting the expression of Nip, a BRCAl-associated centrosomal protein which is involved in microtubule nucleation, spindle formation, chromosomal missegregation and abnormal cytokinesis. We demonstrated that the expression of Nip is strongly associated with ERa and FOXAI level in clinical breast cancer samples with poor clinical outcomes to breast cancer patients. Addition of estrogen in the ER-positive breast cancer cells resulted in elevation of NLP mRNA. Significantly, we identified that estrogen-ERa is capable of regulating Nlp expression through specifically binding ERa to the proximal region and the Estrogen Responsive Elements （ERE） enhancer in the distal region of NLP gene. Reporter assays demonstrated that estrogen directly activated Nlp promoter. ChIP assay results showed that E2-ERa directly bound to the EREs of Nip. Therefore, overexpression of Nip in breast cancer exhibits a hormone-dependent pattern, and estrogen participates in the regulation of genome instability and cell cycle in breast cancer cells partially through transcriptional activation of NLP gene. Overexpression of Nlp enhances the malignant progression of ERa-positive breast cancer cells in vitro, whereas knockdown of Nip suppresses this biological effects in ERa-positive breast cancer ceils. ERa/NIp axis may serve as a promising target against breast cancer.展开更多
文摘The evaluation of estrogenic activities and aryl hydrocarbon receptor (AhR) agonists in water from Three Gorges Reservoir (TGR) China was conducted by in vitro bioassays combined with SPMD-based virtual organisms (VO). VOs were deployed at seven sites in the Three Gorges Reservoir (TGR), China for two periods in 2009. The estrogenic activity was assessed using a rapid yeast estrogen bioassay, based on the expression of a green fluorescent reporter protein (yEGFP). The AhR activity was detected employing rat hepatoma cell line (H4IIE). The results indicate that AhR agonists distributed widely in water of TGR and almost homogenously distributed in most area of TGR. Weak antiestrogenic activities were also found homogenously distributed in water of TGR. Further studies are needed to determine the identities of these estrogenic compounds and AhR agonists and their potential adverse effects on wild biota in TGR.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61308009 and 61405047the China Postdoctoral Science Foundation Funded Project under Grant No 2013M540288+2 种基金the Fundamental Research Funds for the Central Universities under Grant Nos HIT.NSRIF.2014044 and HIT.NSRIF.2015042the Science Fund for Outstanding Youths of Heilongjiang Province under Grant No JQ201310the Heilongjiang Postdoctoral Science Foundation Funded Project under Grant No LBH-Z14085
文摘We demonstrate a cw and actively Q-switched Er：LuAO laser resonantly dual-end-pumped by 1532nm fibre- coupled laser diodes. A maximum cw output power of 1.9W at 1650.3nm is obtained at a pump power of 25.5 W, corresponding to a slope efficiency of 43.3 %. In the Q-switched regime, the maximum pulse energy of 3.51 mJ is reached at a pulse repetition rate of 100 Hz, a pulse duration of 90.5ns and a pump power of 25.5 W. At the repetition rate of 400 Hz, the output energy is 2.12m J, corresponding to a pulse duration of 125.4 ns.
基金supported by National Natural Fund of China(81490753 and 81230047)the National Basic Research Program of China(2015CB553904)
文摘Estrogen Receptor-a （ERa） is the key transcription factor that regulates cell proliferation and homeosta- sis. In this pathway, estrogen plays an important role in genomic instability and cell cycle regulation pro- cesses and the mechanisms of its action are multifaceted. In this study, we showed that estrogen regulates genomic instability through promoting the expression of Nip, a BRCAl-associated centrosomal protein which is involved in microtubule nucleation, spindle formation, chromosomal missegregation and abnormal cytokinesis. We demonstrated that the expression of Nip is strongly associated with ERa and FOXAI level in clinical breast cancer samples with poor clinical outcomes to breast cancer patients. Addition of estrogen in the ER-positive breast cancer cells resulted in elevation of NLP mRNA. Significantly, we identified that estrogen-ERa is capable of regulating Nlp expression through specifically binding ERa to the proximal region and the Estrogen Responsive Elements （ERE） enhancer in the distal region of NLP gene. Reporter assays demonstrated that estrogen directly activated Nlp promoter. ChIP assay results showed that E2-ERa directly bound to the EREs of Nip. Therefore, overexpression of Nip in breast cancer exhibits a hormone-dependent pattern, and estrogen participates in the regulation of genome instability and cell cycle in breast cancer cells partially through transcriptional activation of NLP gene. Overexpression of Nlp enhances the malignant progression of ERa-positive breast cancer cells in vitro, whereas knockdown of Nip suppresses this biological effects in ERa-positive breast cancer ceils. ERa/NIp axis may serve as a promising target against breast cancer.
