Naturally degradable capsule provides a platform for sustained fragrance release.However,practical challenges such as low encapsulation efficiency and difficulty in sustained release are still limited in using fragran...Naturally degradable capsule provides a platform for sustained fragrance release.However,practical challenges such as low encapsulation efficiency and difficulty in sustained release are still limited in using fragranceloaded capsules.In this work,the natural materials sodium alginate and gelatine are dissolved and act as the aqueous phase,lavender is dissolved in caprylic/capric triglyceride(GTCC)as the oil phase,and SiO_(2) nanoparticles with neutralwettability as a solid emulsifier to form O/W Pickering emulsions simultaneously.Finally,multi-core capsules are prepared using the drop injection method with emulsions as templates.The results show that the capsules have been successfully prepared with a spherical morphology and multi-core structure,and the encapsulation rate of multi-core capsules can reach up to 99.6%.In addition,the multi-core capsules possess desirable sustained release performance,the cumulative sustained release rate of fragrance at 25℃over 49 days is only 32.5%.It is attributed to the significant protection of multi-core structure,Pickering emulsion nanoparticle membranes,and hydrogel network shell for encapsulated fragrance.This study is designed to deliver a new strategy for using sustained-release technology with fragrance in food,cosmetics,textiles,and other fields.展开更多
Emulsions are widely used across various industries but often require surfactants for stabilization,leading to complex product formulations and environmental concerns.Continuous preparation of stable emulsions without...Emulsions are widely used across various industries but often require surfactants for stabilization,leading to complex product formulations and environmental concerns.Continuous preparation of stable emulsions without surfactants is still a significant challenge.Herein,a secondary membrane emulsification system was developed to produce stable oil-in-water emulsions under surfactant-free conditions,where the first membrane module employed the cross-flow membrane emulsification to generate the primary emulsion,while the second membrane module employed the premix membrane emulsification to further reduce the droplet size,thereby improving the emulsion stability.The morphology and droplet size of the secondary emulsion significantly depend on the membrane pore size,membrane surface wettability,primary emulsion flow,and primary emulsion quality.The secondary membrane emulsification system demonstrates excellent operational stability over 12 h,with no obvious changes in the emulsion characteristics.The prepared secondary emulsion remains relatively stable up to 10 min without surfactants,significantly superior to the primary emulsion.Furthermore,the developed system successfully produces the Janus emulsion with homogeneous internal phase morphology.This research provides an effective approach for the continuous preparation of stable emulsions without surfactants.展开更多
Herein,a recrystallization approach was used to produce anhydrous sodium sulfate(ASS)microparticles,which are highly efficient and reusable for separating surfactant-stabilized water from water-in-oil emulsions.The AS...Herein,a recrystallization approach was used to produce anhydrous sodium sulfate(ASS)microparticles,which are highly efficient and reusable for separating surfactant-stabilized water from water-in-oil emulsions.The ASS microparticles exhibit distinct morphologies and crystal structures.Remarkably,0.1 g of ASS170 enables the separation of 10 m L of emulsion(water content:0.1 g)with a high separation efficiency of 98.63%.A stepwise separation mechanism,including demulsification and water immobilization in the crystal lattice of ASS,is proposed.The superhydrophilicity of ASS particles enables tiny water droplets to aggregate and merge into larger droplets on their surfaces.This process facilitates the phase transition from ASS to sodium sulfate decahydrate(SSD),during which water molecules are immobilized in the expanded crystal lattice of ASS.SSD particles can be collected to regenerate ASS,retaining the high performance of the original ASS.This unique renewable feature reduces the cost of utilizing ASS and simultaneously prevents secondary pollution.Further economic evaluation reveals that it only costs 66.51USD/m3to purify emulsion with a water content of 10 g/L,significantly lower than previously reported materials.Coupled with a facile and environmentally friendly preparation strategy,this method shows great application potential for water-in-oil emulsion separation and oil purification.展开更多
Rationale:Recurrent pregnancy loss and recurrent implantation failure are frequently linked to immune dysregulation,particularly heightened natural killer(NK)cell activity,cytokine imbalance,and autoantibody presence....Rationale:Recurrent pregnancy loss and recurrent implantation failure are frequently linked to immune dysregulation,particularly heightened natural killer(NK)cell activity,cytokine imbalance,and autoantibody presence.Lipid emulsion therapy,originally designed for parenteral nutrition,is increasingly recognized for its immunomodulatory potential in reproductive medicine.Patient concerns:A 34-year-old woman with five years of infertility,four first-trimester miscarriages,and three failed in vitro fertilization(IVF)cycles despite transfer of morphologically optimal embryos presented for evaluation.Diagnosis:Routine genetic,endocrine,metabolic,thrombophilia,and anatomical investigations were unremarkable.Immunological testing revealed elevated antiphospholipid and anti-thyroid peroxidase(anti-TPO)antibodies,increased NK cell activity,an imbalanced Th1/Th2 cytokine ratio,and raised tumor necrosis factor-alpha(TNF-α),suggesting immune-mediated reproductive dysfunction.Interventions:The patient underwent IVF with transfer of a single euploid blastocyst following intravenous lipid emulsion therapy(20%,100 mL),administered before transfer,on transfer day,and biweekly until 12 weeks.Associated therapies included aspirin,enoxaparin,progesterone,levothyroxine,and supplementation.Outcomes:Serum beta-human chorionic gonadotropin(β-hCG)and ultrasound confirmed pregnancy with subsequent NK cell normalization.The pregnancy was uncomplicated,resulting in spontaneous vaginal delivery of a healthy male infant.Lessons:Lipid emulsion therapy may improve implantation and pregnancy outcomes in immune-mediated recurrent pregnancy loss and recurrent implantation failure,but larger trials are required to validate efficacy and optimize protocols.展开更多
背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏...背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏性鼻炎相关基因表达变化,分析其与程序性细胞死亡的关联,寻找潜在生物标志物和治疗靶点。方法:①从GSE51392、GSE43523和GSE206149数据集中收集过敏性鼻炎患者和对照组的基因表达数据,筛选差异表达基因并进行WGCNA分析。②于2022年3月至2024年5月从新疆医科大学第五附属医院收集10例进行翼管神经切断术治疗的过敏性鼻炎患者和10例健康对照者,采集患者术前术后血液和鼻腔黏膜组织样本。③构建大鼠过敏性鼻炎模型并敲降EP300,并将大鼠分为对照组、模型组、模型+shEP300-NC组、模型+shEP300组。通过ELISA、苏木精-伊红染色、RT-qPCR和Western blot技术分别检测血清炎症因子水平、鼻黏膜组织病理变化及相关基因和蛋白表达。结果与结论:①过敏性鼻炎和对照之间鉴定出43个交集基因;WGCNA发现Green模块与过敏性鼻炎相关性强,通过与程序性细胞死亡相关基因和共同差异表达基因进行交集分析得到关键基因EP300。②与术前相比,过敏性鼻炎患者术后血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著降低,鼻黏膜组织p62、Bcl2的表达显著升高。③与对照组比较,模型组大鼠血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著升高,鼻黏膜组织p62、Bcl2的表达显著降低;与模型组比较,模型+shEP300组大鼠上述指标均呈现相反变化。④结论:EP300可通过调控炎症、自噬与凋亡参与过敏性鼻炎的发生发展过程。展开更多
Eucalyptus staigeriana essential oil(EsEO)has well-known anthelmintic activity in small ruminants.However,its volatility limits its therapeutic action.The aim of this study was to develop a water-in-oil sodium alginat...Eucalyptus staigeriana essential oil(EsEO)has well-known anthelmintic activity in small ruminants.However,its volatility limits its therapeutic action.The aim of this study was to develop a water-in-oil sodium alginatebased nanoemulsion with an effective in vitro effect on the eggs and larvae of Haemonchus contortus,a gastrointestinal parasite of sheep and goats.Four oil-in-water sodium alginate-based emulsions were prepared using a high-energy method with different proportions of Tween 80,EsEO,and sodium alginate(ALG)4%.The physical-chemical characterization included stability,particle size,zeta potential and infrared spectra.The effects of the emulsions were evaluated against H.contortus via the egg hatching test(EHT)and larval development test(LDT).The results showed that the emulsions were stable over 7 days,nanometer scale particles(218.8 to 371.5 nm)predominating and with negative zeta potentials(−28.9 to−46.9 mV).All four emulsions were more effective than EsEO in the EHT,with 50%effective concentrations(EC50)of 0.088 to 0.15 mg/mL for the emulsions and 0.308 mg/mL for EsEO.However,in the LDT,the emulsions and essential oil had similar effects,with EC50 values of 3.91 to 4.60 mg/mL for the emulsions and 4.17 mg/mL for EsEO.Emulsion 2,with low Tween 80/EsEO and ALG/EsEO ratios,was considered better overall in terms of physical,chemical and anthelmintic assessment and is a promising candidate for further in vivo assays against adult H.contortus.展开更多
针对现有桥梁护栏存在的防护性能不足、大规模改造需求、复杂施工条件等挑战,提出了“强-弱结合、刚-柔协同”的功能梯度型超高性能混凝土-发泡聚苯乙烯(Ultra high performance concrete-Expandable polystyrene,UHPC-EPS)夹芯护栏体系...针对现有桥梁护栏存在的防护性能不足、大规模改造需求、复杂施工条件等挑战,提出了“强-弱结合、刚-柔协同”的功能梯度型超高性能混凝土-发泡聚苯乙烯(Ultra high performance concrete-Expandable polystyrene,UHPC-EPS)夹芯护栏体系,通过LS-Dyna数值仿真技术,模拟了该护栏在小型客车、大型客车、大型货车碰撞场景下的轨迹偏移规律与损伤演化过程,并与现浇混凝土护栏开展了大型货车冲击工况下的力学性能对比研究。结果表明:功能梯度型UHPC-EPS夹芯护栏兼具优异的车辆轨迹导向能力和运行稳定性调控性能,同时具有强抗穿透特性与便捷的后期修复优势;在大型货车冲击工况下,UHPC-EPS夹芯护栏的峰值撞击力较现浇混凝土护栏降低了约12%,峰值剪力降低了约35%;配套研发的标准化施工工艺采用工厂预制、现场装配、一次成型的快速部署,具有显著的工程适配性与产业化推广价值。展开更多
文摘Naturally degradable capsule provides a platform for sustained fragrance release.However,practical challenges such as low encapsulation efficiency and difficulty in sustained release are still limited in using fragranceloaded capsules.In this work,the natural materials sodium alginate and gelatine are dissolved and act as the aqueous phase,lavender is dissolved in caprylic/capric triglyceride(GTCC)as the oil phase,and SiO_(2) nanoparticles with neutralwettability as a solid emulsifier to form O/W Pickering emulsions simultaneously.Finally,multi-core capsules are prepared using the drop injection method with emulsions as templates.The results show that the capsules have been successfully prepared with a spherical morphology and multi-core structure,and the encapsulation rate of multi-core capsules can reach up to 99.6%.In addition,the multi-core capsules possess desirable sustained release performance,the cumulative sustained release rate of fragrance at 25℃over 49 days is only 32.5%.It is attributed to the significant protection of multi-core structure,Pickering emulsion nanoparticle membranes,and hydrogel network shell for encapsulated fragrance.This study is designed to deliver a new strategy for using sustained-release technology with fragrance in food,cosmetics,textiles,and other fields.
基金Financial support from the National Key Research and Development Program (2024YFA1510003)the National Natural Science Foundation (U24B20197,22278209,U23A20117,22178165,22208149)+1 种基金the Jiangsu Province Natural Science Foundation(BK20220354)the State Key Laboratory of Materials-Oriented Chemical Engineering (SKL-MCE-23A11) of China are gratefully acknowledged。
文摘Emulsions are widely used across various industries but often require surfactants for stabilization,leading to complex product formulations and environmental concerns.Continuous preparation of stable emulsions without surfactants is still a significant challenge.Herein,a secondary membrane emulsification system was developed to produce stable oil-in-water emulsions under surfactant-free conditions,where the first membrane module employed the cross-flow membrane emulsification to generate the primary emulsion,while the second membrane module employed the premix membrane emulsification to further reduce the droplet size,thereby improving the emulsion stability.The morphology and droplet size of the secondary emulsion significantly depend on the membrane pore size,membrane surface wettability,primary emulsion flow,and primary emulsion quality.The secondary membrane emulsification system demonstrates excellent operational stability over 12 h,with no obvious changes in the emulsion characteristics.The prepared secondary emulsion remains relatively stable up to 10 min without surfactants,significantly superior to the primary emulsion.Furthermore,the developed system successfully produces the Janus emulsion with homogeneous internal phase morphology.This research provides an effective approach for the continuous preparation of stable emulsions without surfactants.
基金financially supported by the High-level talent research start-up project of Chongqing Technology and Business University(No.2356007)the Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJQN202400809)Special Project for Performance Incentive and Guidance of Research Institutions in Chongqing(No.CSTB2023JXJL-YFX0030)。
文摘Herein,a recrystallization approach was used to produce anhydrous sodium sulfate(ASS)microparticles,which are highly efficient and reusable for separating surfactant-stabilized water from water-in-oil emulsions.The ASS microparticles exhibit distinct morphologies and crystal structures.Remarkably,0.1 g of ASS170 enables the separation of 10 m L of emulsion(water content:0.1 g)with a high separation efficiency of 98.63%.A stepwise separation mechanism,including demulsification and water immobilization in the crystal lattice of ASS,is proposed.The superhydrophilicity of ASS particles enables tiny water droplets to aggregate and merge into larger droplets on their surfaces.This process facilitates the phase transition from ASS to sodium sulfate decahydrate(SSD),during which water molecules are immobilized in the expanded crystal lattice of ASS.SSD particles can be collected to regenerate ASS,retaining the high performance of the original ASS.This unique renewable feature reduces the cost of utilizing ASS and simultaneously prevents secondary pollution.Further economic evaluation reveals that it only costs 66.51USD/m3to purify emulsion with a water content of 10 g/L,significantly lower than previously reported materials.Coupled with a facile and environmentally friendly preparation strategy,this method shows great application potential for water-in-oil emulsion separation and oil purification.
文摘Rationale:Recurrent pregnancy loss and recurrent implantation failure are frequently linked to immune dysregulation,particularly heightened natural killer(NK)cell activity,cytokine imbalance,and autoantibody presence.Lipid emulsion therapy,originally designed for parenteral nutrition,is increasingly recognized for its immunomodulatory potential in reproductive medicine.Patient concerns:A 34-year-old woman with five years of infertility,four first-trimester miscarriages,and three failed in vitro fertilization(IVF)cycles despite transfer of morphologically optimal embryos presented for evaluation.Diagnosis:Routine genetic,endocrine,metabolic,thrombophilia,and anatomical investigations were unremarkable.Immunological testing revealed elevated antiphospholipid and anti-thyroid peroxidase(anti-TPO)antibodies,increased NK cell activity,an imbalanced Th1/Th2 cytokine ratio,and raised tumor necrosis factor-alpha(TNF-α),suggesting immune-mediated reproductive dysfunction.Interventions:The patient underwent IVF with transfer of a single euploid blastocyst following intravenous lipid emulsion therapy(20%,100 mL),administered before transfer,on transfer day,and biweekly until 12 weeks.Associated therapies included aspirin,enoxaparin,progesterone,levothyroxine,and supplementation.Outcomes:Serum beta-human chorionic gonadotropin(β-hCG)and ultrasound confirmed pregnancy with subsequent NK cell normalization.The pregnancy was uncomplicated,resulting in spontaneous vaginal delivery of a healthy male infant.Lessons:Lipid emulsion therapy may improve implantation and pregnancy outcomes in immune-mediated recurrent pregnancy loss and recurrent implantation failure,but larger trials are required to validate efficacy and optimize protocols.
文摘背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏性鼻炎相关基因表达变化,分析其与程序性细胞死亡的关联,寻找潜在生物标志物和治疗靶点。方法:①从GSE51392、GSE43523和GSE206149数据集中收集过敏性鼻炎患者和对照组的基因表达数据,筛选差异表达基因并进行WGCNA分析。②于2022年3月至2024年5月从新疆医科大学第五附属医院收集10例进行翼管神经切断术治疗的过敏性鼻炎患者和10例健康对照者,采集患者术前术后血液和鼻腔黏膜组织样本。③构建大鼠过敏性鼻炎模型并敲降EP300,并将大鼠分为对照组、模型组、模型+shEP300-NC组、模型+shEP300组。通过ELISA、苏木精-伊红染色、RT-qPCR和Western blot技术分别检测血清炎症因子水平、鼻黏膜组织病理变化及相关基因和蛋白表达。结果与结论:①过敏性鼻炎和对照之间鉴定出43个交集基因;WGCNA发现Green模块与过敏性鼻炎相关性强,通过与程序性细胞死亡相关基因和共同差异表达基因进行交集分析得到关键基因EP300。②与术前相比,过敏性鼻炎患者术后血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著降低,鼻黏膜组织p62、Bcl2的表达显著升高。③与对照组比较,模型组大鼠血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著升高,鼻黏膜组织p62、Bcl2的表达显著降低;与模型组比较,模型+shEP300组大鼠上述指标均呈现相反变化。④结论:EP300可通过调控炎症、自噬与凋亡参与过敏性鼻炎的发生发展过程。
基金supported by Conselho Nacional de Desenvolvimento Cientifico eTecnologico(CNPq)(grant No.142165/2018-2)Mr.Araujo-Filho had a doctoral scholarship and Dr.Bevilaqua has a research fellowship(grant No.305911/2019-8)from CNPq.
文摘Eucalyptus staigeriana essential oil(EsEO)has well-known anthelmintic activity in small ruminants.However,its volatility limits its therapeutic action.The aim of this study was to develop a water-in-oil sodium alginatebased nanoemulsion with an effective in vitro effect on the eggs and larvae of Haemonchus contortus,a gastrointestinal parasite of sheep and goats.Four oil-in-water sodium alginate-based emulsions were prepared using a high-energy method with different proportions of Tween 80,EsEO,and sodium alginate(ALG)4%.The physical-chemical characterization included stability,particle size,zeta potential and infrared spectra.The effects of the emulsions were evaluated against H.contortus via the egg hatching test(EHT)and larval development test(LDT).The results showed that the emulsions were stable over 7 days,nanometer scale particles(218.8 to 371.5 nm)predominating and with negative zeta potentials(−28.9 to−46.9 mV).All four emulsions were more effective than EsEO in the EHT,with 50%effective concentrations(EC50)of 0.088 to 0.15 mg/mL for the emulsions and 0.308 mg/mL for EsEO.However,in the LDT,the emulsions and essential oil had similar effects,with EC50 values of 3.91 to 4.60 mg/mL for the emulsions and 4.17 mg/mL for EsEO.Emulsion 2,with low Tween 80/EsEO and ALG/EsEO ratios,was considered better overall in terms of physical,chemical and anthelmintic assessment and is a promising candidate for further in vivo assays against adult H.contortus.
文摘针对现有桥梁护栏存在的防护性能不足、大规模改造需求、复杂施工条件等挑战,提出了“强-弱结合、刚-柔协同”的功能梯度型超高性能混凝土-发泡聚苯乙烯(Ultra high performance concrete-Expandable polystyrene,UHPC-EPS)夹芯护栏体系,通过LS-Dyna数值仿真技术,模拟了该护栏在小型客车、大型客车、大型货车碰撞场景下的轨迹偏移规律与损伤演化过程,并与现浇混凝土护栏开展了大型货车冲击工况下的力学性能对比研究。结果表明:功能梯度型UHPC-EPS夹芯护栏兼具优异的车辆轨迹导向能力和运行稳定性调控性能,同时具有强抗穿透特性与便捷的后期修复优势;在大型货车冲击工况下,UHPC-EPS夹芯护栏的峰值撞击力较现浇混凝土护栏降低了约12%,峰值剪力降低了约35%;配套研发的标准化施工工艺采用工厂预制、现场装配、一次成型的快速部署,具有显著的工程适配性与产业化推广价值。
