背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏...背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏性鼻炎相关基因表达变化,分析其与程序性细胞死亡的关联,寻找潜在生物标志物和治疗靶点。方法:①从GSE51392、GSE43523和GSE206149数据集中收集过敏性鼻炎患者和对照组的基因表达数据,筛选差异表达基因并进行WGCNA分析。②于2022年3月至2024年5月从新疆医科大学第五附属医院收集10例进行翼管神经切断术治疗的过敏性鼻炎患者和10例健康对照者,采集患者术前术后血液和鼻腔黏膜组织样本。③构建大鼠过敏性鼻炎模型并敲降EP300,并将大鼠分为对照组、模型组、模型+shEP300-NC组、模型+shEP300组。通过ELISA、苏木精-伊红染色、RT-qPCR和Western blot技术分别检测血清炎症因子水平、鼻黏膜组织病理变化及相关基因和蛋白表达。结果与结论:①过敏性鼻炎和对照之间鉴定出43个交集基因;WGCNA发现Green模块与过敏性鼻炎相关性强,通过与程序性细胞死亡相关基因和共同差异表达基因进行交集分析得到关键基因EP300。②与术前相比,过敏性鼻炎患者术后血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著降低,鼻黏膜组织p62、Bcl2的表达显著升高。③与对照组比较,模型组大鼠血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著升高,鼻黏膜组织p62、Bcl2的表达显著降低;与模型组比较,模型+shEP300组大鼠上述指标均呈现相反变化。④结论:EP300可通过调控炎症、自噬与凋亡参与过敏性鼻炎的发生发展过程。展开更多
详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;...详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;尺寸偏差特别是对管端内径、内径不圆度要求更严;对探伤检验的人员资质、校样要求和探伤方式进行了详细规定。解读GS EP PLR 205标准有利于制造商对标一线要求,寻找差距,改进工艺和控制能力,为进入国际高端市场做准备。展开更多
Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal ...Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal insulation materials are key to achieving successful ITP-Coring.Materials composed of hollow glass microspheres(HGMs)as fillers and epoxy resin(EP)as the matrix are promising thermal insulation materials for application in ITP-Coring to exploit deep resources.The compressive mechanical properties of these materials significantly influence their applicability and reliability.However,few studies have focused on the compressive mechanical behavior of the materials under high-temperature and high-pressure(HTHP)coupled conditions.Therefore,compressive mechanical tests on materials under temperatures and pressures of up to 150℃and 140 MPa were conducted innovatively.The compressive stress-strain curves of the materials were divided into three stages:elastic,yield,and failure,at temperatures ranging from 25℃to 100℃.Increasing temperature and pressure resulted in a decrease in compressive mechanical properties.Notably,high pressure damaged the HGMs,increasing compressive strain as the temperature rose.Additionally,the compressive failure mode shifted from compound failure to shear failure at different thresholds of HTHP conditions.Finally,a constitutive model of compressive mechanics that considered multiple coupled factors was established,demonstrating good agreement with the experimental results.These findings provide both experimental and theoretical support for the optimization and engineering application of HGMs/EP materials.展开更多
文摘背景:E1A结合蛋白300(E1A Binding Protein 300,EP300)作为一种多功能组蛋白乙酰转移酶,广泛参与基因表达调控、细胞生长及分化等生物学过程,并与多种炎症及免疫相关疾病相关,但它在过敏性鼻炎发病中的具体功能尚不明确。目的:探究过敏性鼻炎相关基因表达变化,分析其与程序性细胞死亡的关联,寻找潜在生物标志物和治疗靶点。方法:①从GSE51392、GSE43523和GSE206149数据集中收集过敏性鼻炎患者和对照组的基因表达数据,筛选差异表达基因并进行WGCNA分析。②于2022年3月至2024年5月从新疆医科大学第五附属医院收集10例进行翼管神经切断术治疗的过敏性鼻炎患者和10例健康对照者,采集患者术前术后血液和鼻腔黏膜组织样本。③构建大鼠过敏性鼻炎模型并敲降EP300,并将大鼠分为对照组、模型组、模型+shEP300-NC组、模型+shEP300组。通过ELISA、苏木精-伊红染色、RT-qPCR和Western blot技术分别检测血清炎症因子水平、鼻黏膜组织病理变化及相关基因和蛋白表达。结果与结论:①过敏性鼻炎和对照之间鉴定出43个交集基因;WGCNA发现Green模块与过敏性鼻炎相关性强,通过与程序性细胞死亡相关基因和共同差异表达基因进行交集分析得到关键基因EP300。②与术前相比,过敏性鼻炎患者术后血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著降低,鼻黏膜组织p62、Bcl2的表达显著升高。③与对照组比较,模型组大鼠血清白细胞介素4、白细胞介素5、白细胞介素13水平及鼻黏膜组织EP300、LC3B、Beclin1、cleaved-Caspase的表达显著升高,鼻黏膜组织p62、Bcl2的表达显著降低;与模型组比较,模型+shEP300组大鼠上述指标均呈现相反变化。④结论:EP300可通过调控炎症、自噬与凋亡参与过敏性鼻炎的发生发展过程。
文摘详细解读道达尔GS EP PLR 205标准的主要内容。分析认为,与API Spec 5L标准相比,GS EP PLR205标准对化学成分要求更严,提出了偏析控制的要求;对性能如纵横向拉伸、冲击、硬度、氢致开裂和硫化物应力腐蚀开裂和应变时效等要求做了规定;尺寸偏差特别是对管端内径、内径不圆度要求更严;对探伤检验的人员资质、校样要求和探伤方式进行了详细规定。解读GS EP PLR 205标准有利于制造商对标一线要求,寻找差距,改进工艺和控制能力,为进入国际高端市场做准备。
基金supported by the National Key Research and Development Program of China(Grant No.2023YFB2390200)the National Natural Science Foundation of China(Grant No.52304033)the Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization(Grant No.DESGEEU-2023-10).
文摘Deep oil and gas reservoirs exist under high-temperature conditions.In situ temperature-preserved coring(ITP-Coring)is an innovative and crucial method for evaluating and exploiting deep oil and gas resources.Thermal insulation materials are key to achieving successful ITP-Coring.Materials composed of hollow glass microspheres(HGMs)as fillers and epoxy resin(EP)as the matrix are promising thermal insulation materials for application in ITP-Coring to exploit deep resources.The compressive mechanical properties of these materials significantly influence their applicability and reliability.However,few studies have focused on the compressive mechanical behavior of the materials under high-temperature and high-pressure(HTHP)coupled conditions.Therefore,compressive mechanical tests on materials under temperatures and pressures of up to 150℃and 140 MPa were conducted innovatively.The compressive stress-strain curves of the materials were divided into three stages:elastic,yield,and failure,at temperatures ranging from 25℃to 100℃.Increasing temperature and pressure resulted in a decrease in compressive mechanical properties.Notably,high pressure damaged the HGMs,increasing compressive strain as the temperature rose.Additionally,the compressive failure mode shifted from compound failure to shear failure at different thresholds of HTHP conditions.Finally,a constitutive model of compressive mechanics that considered multiple coupled factors was established,demonstrating good agreement with the experimental results.These findings provide both experimental and theoretical support for the optimization and engineering application of HGMs/EP materials.
