EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreas...EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development.展开更多
据外媒报道，瑞士国家联邦实验室（Empa）能源转换材料系（MateriaIs for Energy Conversion Department）的两位研究人员RUben·Simon Kohnel与Darid Reber研发了新方案，旨在解决以下难题：确保含电解质的盐类为液态形式，同时确...据外媒报道，瑞士国家联邦实验室（Empa）能源转换材料系（MateriaIs for Energy Conversion Department）的两位研究人员RUben·Simon Kohnel与Darid Reber研发了新方案，旨在解决以下难题：确保含电解质的盐类为液态形式，同时确保其达到高浓度，且不合“多余的”水分。展开更多
基金This project was funded by the 973 Program (2006CB101803)the National Natural Sciences Foundation of China (30871935)
文摘EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development.
文摘据外媒报道，瑞士国家联邦实验室（Empa）能源转换材料系（MateriaIs for Energy Conversion Department）的两位研究人员RUben·Simon Kohnel与Darid Reber研发了新方案，旨在解决以下难题：确保含电解质的盐类为液态形式，同时确保其达到高浓度，且不合“多余的”水分。
