目的检测鼻腔鼻窦内翻性乳头状瘤中上皮细胞膜蛋白1(EMP1)、环氧化酶2(COX-2)及Stathmin蛋白的表达情况,分析其与上皮化生程度的相关性。方法选取2015年1月~2016年3月在本院就诊的鼻腔鼻窦内翻性乳头状瘤患者28例作为实验组,20例肥厚性...目的检测鼻腔鼻窦内翻性乳头状瘤中上皮细胞膜蛋白1(EMP1)、环氧化酶2(COX-2)及Stathmin蛋白的表达情况,分析其与上皮化生程度的相关性。方法选取2015年1月~2016年3月在本院就诊的鼻腔鼻窦内翻性乳头状瘤患者28例作为实验组,20例肥厚性鼻炎患者的下鼻甲黏膜组织作为对照组,采用免疫组化技术和q RT-PCR检测鼻腔鼻窦内翻性乳头状瘤、下鼻甲黏膜组织EMP1、COX-2以及Stathmin的表达情况,并比较三种因子在不同组织中的表达情况。结果实验组EMP1蛋白表达的阳性率显著低于对照组(P<0.05),COX-2及Stathmin蛋白表达的阳性率显著高于对照组(P<0.05)。实验组EMP1的m RNA水平显著低于对照组(P<0.05),COX-2及Stathmin m RNA水平显著高于对照组(P<0.05)。EMP1蛋白表达水平与上皮化生的严重程度呈负相关,COX-2及Stathmin蛋白表达水平与上皮化生的严重程度呈正相关。结论鼻腔鼻窦内翻性乳头状瘤中EMP1、COX-2及Stathmin蛋白的异常表达与上皮化生程度有关,联合检测EMP1、COX-2及Stathmin蛋白表达可评估鼻腔鼻窦内翻性乳头状瘤上皮异常重塑情况。展开更多
Pancreatic cancer(PC)is a highly malignant neoplasm of the digestive system.The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related...Pancreatic cancer(PC)is a highly malignant neoplasm of the digestive system.The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related factor Epithelial membrane protein 1(EMP1)in PC progression.We established a prognostic model pertinent to the aging process that could be applied in postoperative PC patients.In vitro assays were employed to elucidate the impact of EMP1 on PC cell function.We employed lentiviral vectors for both knockdown and overexpression of EMP1 in Panc02 cells,followed by the establishment of subcutaneous,pulmonary metastasis,and orthotopic pancreatic liver metastasis models in mice.Using tissue microarrays,we evaluated the expression of EMP1 and its downstream entities,and then conducted clinical correlation analysis.A predictive Age-Related Score(ARS)system based on age-associated prognostic genes was developed,offering precise prognostic predictions for postoperative PC patients,which could be applied well at the single-cell level,showing diverse aging,epithelial–mesenchymal transition(EMT),cell migration,cell proliferation,and PI3K/AKT signaling activity in high and low ARS risk cells.EMP1 was identified as a pivotal molecule in the ARS system and is associated with poor prognosis.Besides,EMP1 could enhance the proliferation,migration,and invasion of PC cells both in vitro and in vivo by augmenting the PI3K/AKT signaling cascade.In essence,this research formulated an aging-centric prognostic model for postoperative PC and pinpointed EMP1 as an oncogenic factor facilitating tumor cell EMT during the aging trajectory in resectable PC patients.展开更多
文摘目的检测鼻腔鼻窦内翻性乳头状瘤中上皮细胞膜蛋白1(EMP1)、环氧化酶2(COX-2)及Stathmin蛋白的表达情况,分析其与上皮化生程度的相关性。方法选取2015年1月~2016年3月在本院就诊的鼻腔鼻窦内翻性乳头状瘤患者28例作为实验组,20例肥厚性鼻炎患者的下鼻甲黏膜组织作为对照组,采用免疫组化技术和q RT-PCR检测鼻腔鼻窦内翻性乳头状瘤、下鼻甲黏膜组织EMP1、COX-2以及Stathmin的表达情况,并比较三种因子在不同组织中的表达情况。结果实验组EMP1蛋白表达的阳性率显著低于对照组(P<0.05),COX-2及Stathmin蛋白表达的阳性率显著高于对照组(P<0.05)。实验组EMP1的m RNA水平显著低于对照组(P<0.05),COX-2及Stathmin m RNA水平显著高于对照组(P<0.05)。EMP1蛋白表达水平与上皮化生的严重程度呈负相关,COX-2及Stathmin蛋白表达水平与上皮化生的严重程度呈正相关。结论鼻腔鼻窦内翻性乳头状瘤中EMP1、COX-2及Stathmin蛋白的异常表达与上皮化生程度有关,联合检测EMP1、COX-2及Stathmin蛋白表达可评估鼻腔鼻窦内翻性乳头状瘤上皮异常重塑情况。
基金sponsored by Natural Science Foundation of Chongqing,China(No.CSTB2022NSCQ-MSX1339 to J.F.Z.CSTB2022NSCQ-MSX1273 to J.Y.G.),Foundation of Key Laboratory of Tumor Immunology and Pathology(Army Medical University)+3 种基金Ministry of Education(No.2023jsz910 to J.F.Z.)National Natural Science Foundation of China(No.82203165 to J.Y.G.,82072723,82103249 to H.Z.W.)Chongqing medical scientific research project(Joint project of Chongqing Health Commission and Science and Technology Bureau)(No.2022MSXM031 to J.F.X.)Chongqing Technology Innovation and Application Development Special Key Project(No.CSTB2022TIAD-KPX0170 to H.Z.W.).
文摘Pancreatic cancer(PC)is a highly malignant neoplasm of the digestive system.The primary objective of this investigation is to elucidate the intricate mechanisms underlying the role of the aging process and the related factor Epithelial membrane protein 1(EMP1)in PC progression.We established a prognostic model pertinent to the aging process that could be applied in postoperative PC patients.In vitro assays were employed to elucidate the impact of EMP1 on PC cell function.We employed lentiviral vectors for both knockdown and overexpression of EMP1 in Panc02 cells,followed by the establishment of subcutaneous,pulmonary metastasis,and orthotopic pancreatic liver metastasis models in mice.Using tissue microarrays,we evaluated the expression of EMP1 and its downstream entities,and then conducted clinical correlation analysis.A predictive Age-Related Score(ARS)system based on age-associated prognostic genes was developed,offering precise prognostic predictions for postoperative PC patients,which could be applied well at the single-cell level,showing diverse aging,epithelial–mesenchymal transition(EMT),cell migration,cell proliferation,and PI3K/AKT signaling activity in high and low ARS risk cells.EMP1 was identified as a pivotal molecule in the ARS system and is associated with poor prognosis.Besides,EMP1 could enhance the proliferation,migration,and invasion of PC cells both in vitro and in vivo by augmenting the PI3K/AKT signaling cascade.In essence,this research formulated an aging-centric prognostic model for postoperative PC and pinpointed EMP1 as an oncogenic factor facilitating tumor cell EMT during the aging trajectory in resectable PC patients.
