Contrary to freezing preservation and formalin embalming,Thiel embalmed cadaver presents soft texture and color very close to that of living organism,and many applications based on Thiel embalmed cadavers have been re...Contrary to freezing preservation and formalin embalming,Thiel embalmed cadaver presents soft texture and color very close to that of living organism,and many applications based on Thiel embalmed cadavers have been reported.However,Thiel embalmed cadavers cannot be used as reliable evaluation model for radiofrequency ablation(RFA)due to dramatic changes of electrical conductivity in the embalmed tissue.To address this issue,we investigated various modifications of the original Thiel embalming solution.By altering the chemicals' species and concentration we figured out a formula that can greatly reduce the embalming fluid's electrical conductivity without significantly compromising the 18-day embalmed kidney samples' suppleness and color.We also investigated a two-stage embalming technique by first submerging the kidney sample into original Thiel's tank fluid for 28 days,then the sample was withdrawn from the tank fluid and placed into modified dilution fluids for additional two weeks.Stiffening and discoloration occurred in these diluted samples implying the reversibility of Thiel-embalmed tissues' suppleness and color with the removal of the strong electrolytes.This study presents a modified embalming method which could be used for RPA evaluation and also helps our understanding of the mechanism of embalmment process.展开更多
Putrefaction and decay of preserved cadaver and body parts is one of the major issues that the faculties of medicines in various third world countries are facing. In this study we focus on the meaning of body preserva...Putrefaction and decay of preserved cadaver and body parts is one of the major issues that the faculties of medicines in various third world countries are facing. In this study we focus on the meaning of body preservation, causes of putrefaction and we highlight our personal experiences at the Faculty of Medicine in Prince Sattam bin Abdulaziz University in KSA, in preserving the body cadavers. We studied different physical and chemical methods of preservation of cadavers and body parts at the faculty and their efficiency in preventing bacteria and fungi that cause putrefaction and decay.展开更多
Introduction:Storage of specimens sampled from human remains for pathological testing,embalming for burial purposes,and for human identification often requires formalin fixation and/or paraffin embedding.Current knowl...Introduction:Storage of specimens sampled from human remains for pathological testing,embalming for burial purposes,and for human identification often requires formalin fixation and/or paraffin embedding.Current knowledge in molecular biology techniques and forensic DNA analysis makes it possible to optimize the extraction of amplifiable DNA from formalin-fixed tissues by improving the pre-treatment,optimizing the digestion condition of proteinase K,simplifying the extraction protocol and purifying the extracted DNA with optimized volumes of alcohol.Aim:This research sought to extract amplifiable DNA from thirteen brain,bone marrow and cartilage samples from four formalin embalmed human cadavers.Materials and Methods:Brain,cartilage and bone marrow samples were taken from four different cadavers at autopsy at the Ghana Police Hospital mortuary in Accra,Ghana sixty-two days after embalming.An optimized preparation and DNA extraction protocol was carried out on all the samples.Brain samples were also taken from a non-formalin treated fifth cadaver of known STR profile,and standard DNA extraction performed to serve as positive control.Results:Our optimized protocol yielded detectable quantities of DNA from the samples when quantified with the 7500 Real-Time PCR equipment.The extracted DNA also yielded full STR profiles with varying peak heights for forensic identification purposes.The measured degradation indexes of the DNA samples were greater than 1.0,with peak heights of generated STR profiles above the limits of detection of the 3500 genetic analyzer.Conclusion:Our current study demonstrated an optimized method of DNA extraction from tissues(brain,cartilage and bone marrow)sampled from formalin embalmed human cadavers.The optimized protocol reduced the concentration of formalin fixation residues in extracted DNA from formalin-fixed tissues,thereby improving the amplification efficiency for STR profiling.Brain,bone marrow and cartilages can be a good source of DNA from embalmed and degraded human remains,though for skeletonized human remains together with teeth and long bones.展开更多
文摘Contrary to freezing preservation and formalin embalming,Thiel embalmed cadaver presents soft texture and color very close to that of living organism,and many applications based on Thiel embalmed cadavers have been reported.However,Thiel embalmed cadavers cannot be used as reliable evaluation model for radiofrequency ablation(RFA)due to dramatic changes of electrical conductivity in the embalmed tissue.To address this issue,we investigated various modifications of the original Thiel embalming solution.By altering the chemicals' species and concentration we figured out a formula that can greatly reduce the embalming fluid's electrical conductivity without significantly compromising the 18-day embalmed kidney samples' suppleness and color.We also investigated a two-stage embalming technique by first submerging the kidney sample into original Thiel's tank fluid for 28 days,then the sample was withdrawn from the tank fluid and placed into modified dilution fluids for additional two weeks.Stiffening and discoloration occurred in these diluted samples implying the reversibility of Thiel-embalmed tissues' suppleness and color with the removal of the strong electrolytes.This study presents a modified embalming method which could be used for RPA evaluation and also helps our understanding of the mechanism of embalmment process.
文摘Putrefaction and decay of preserved cadaver and body parts is one of the major issues that the faculties of medicines in various third world countries are facing. In this study we focus on the meaning of body preservation, causes of putrefaction and we highlight our personal experiences at the Faculty of Medicine in Prince Sattam bin Abdulaziz University in KSA, in preserving the body cadavers. We studied different physical and chemical methods of preservation of cadavers and body parts at the faculty and their efficiency in preventing bacteria and fungi that cause putrefaction and decay.
文摘Introduction:Storage of specimens sampled from human remains for pathological testing,embalming for burial purposes,and for human identification often requires formalin fixation and/or paraffin embedding.Current knowledge in molecular biology techniques and forensic DNA analysis makes it possible to optimize the extraction of amplifiable DNA from formalin-fixed tissues by improving the pre-treatment,optimizing the digestion condition of proteinase K,simplifying the extraction protocol and purifying the extracted DNA with optimized volumes of alcohol.Aim:This research sought to extract amplifiable DNA from thirteen brain,bone marrow and cartilage samples from four formalin embalmed human cadavers.Materials and Methods:Brain,cartilage and bone marrow samples were taken from four different cadavers at autopsy at the Ghana Police Hospital mortuary in Accra,Ghana sixty-two days after embalming.An optimized preparation and DNA extraction protocol was carried out on all the samples.Brain samples were also taken from a non-formalin treated fifth cadaver of known STR profile,and standard DNA extraction performed to serve as positive control.Results:Our optimized protocol yielded detectable quantities of DNA from the samples when quantified with the 7500 Real-Time PCR equipment.The extracted DNA also yielded full STR profiles with varying peak heights for forensic identification purposes.The measured degradation indexes of the DNA samples were greater than 1.0,with peak heights of generated STR profiles above the limits of detection of the 3500 genetic analyzer.Conclusion:Our current study demonstrated an optimized method of DNA extraction from tissues(brain,cartilage and bone marrow)sampled from formalin embalmed human cadavers.The optimized protocol reduced the concentration of formalin fixation residues in extracted DNA from formalin-fixed tissues,thereby improving the amplification efficiency for STR profiling.Brain,bone marrow and cartilages can be a good source of DNA from embalmed and degraded human remains,though for skeletonized human remains together with teeth and long bones.
