Directional three-dimensional carbon-based foams are emerging as highly attractive candidates for promising electromagnetic wave absorbing materials(EWAMs)thanks to their unique architecture,but their construction usu...Directional three-dimensional carbon-based foams are emerging as highly attractive candidates for promising electromagnetic wave absorbing materials(EWAMs)thanks to their unique architecture,but their construction usually involves complex procedures and extremely depends on unidirectional freezing technique.Herein,we propose a groundbreaking approach that leverages the assemblies of salting-out protein induced by ammonium metatungstate(AM)as the precursor,and then acquire directional three-dimensional carbon-based foams through simple pyrolysis.The electrostatic interaction between AM and protein ensures well dispersion of WC_(1−x)nanoparticles on carbon frameworks.The content of WC_(1−x)nanoparticles can be rationally regulated by AM dosage,and it also affects the electromagnetic(EM)properties of final carbon-based foams.The optimized foam exhibits exceptional EM absorption performance,achieving a remarkable minimum reflection loss of−72.0 dB and an effective absorption bandwidth of 6.3 GHz when EM wave propagates parallel to the directional pores.Such performance benefits from the synergistic effects of macroporous architecture and compositional design.Although there is a directional dependence of EM absorption,radar stealth simulation demonstrates that these foams can still promise considerable reduction in radar cross section with the change of incident angle.Moreover,COMSOL simulation further identifies their good performance in preventing EM interference among different electronic components.展开更多
旨在探讨EM微生物菌剂与牛、羊粪配施对高海拔地区燕麦产量、品质及土壤质量的影响。以‘青海甜燕麦’为材料,比较了不同剂量的EM菌剂(EM1、EM2、EM3、EM4、EM5)与牛、羊粪配施效果,并以不施肥作为对照(CK)。在生长季节(5—10月)观察了...旨在探讨EM微生物菌剂与牛、羊粪配施对高海拔地区燕麦产量、品质及土壤质量的影响。以‘青海甜燕麦’为材料,比较了不同剂量的EM菌剂(EM1、EM2、EM3、EM4、EM5)与牛、羊粪配施效果,并以不施肥作为对照(CK)。在生长季节(5—10月)观察了各处理对燕麦生长发育、产量、品质、土壤养分及酶活性的影响。结果表明,施用EM菌剂显著促进了燕麦生长,尤其在株高、根总长、旗叶叶面积和鲜质量上,其中EM4处理的株高最高,较CK增加20.11%。光合作用方面,增施EM菌剂提高了燕麦SPAD值、光合速率、蒸腾速率、气孔导度和胞内CO_(2)浓度,其中EM3和EM4处理的净光合速率均超过13μmol/(m^(2)·s),较CK分别增加34.38%和33.57%。营养品质分析显示,EM3处理的粗蛋白和可溶性糖含量分别为7.13%和10.65%,较CK显著提高38.71%和32.46%,且总可消化养分含量均在60%以上,显著优于CK。在饲草产量方面,EM3处理的鲜草和干草产量最高,分别为28492.00和10928.80 kg/hm^(2),较CK分别增加28.57%和54.36%。土壤质量方面,施用EM菌剂后,土壤有机碳和全氮含量显著增加,EM3处理的全氮含量最高,较CK提高18.23%,同时土壤电导率和pH下降。土壤β-葡萄糖甘梅和N-乙酰-β-D-氨基葡萄糖苷酶活性在EM3处理达到峰值,分别较CK处理显著提高60.31%和70.68%。隶属函数综合评价分析显示,各处理对燕麦生长和土壤质量的改善效果依次为EM3>EM4>EM5>EM2>EM1>CK,表明EM3处理效果最佳。因此,在高海拔地区每公顷施用15.00 kg EM菌剂+18000 kg的牛羊粪组合效果最佳,显著促进了燕麦的生长发育,提高了其营养品质,并有效改善了土壤养分及酶活性。建议在高海拔地区推广该施肥方案,以提升燕麦的产量和土壤健康,促进高海拔地区燕麦饲草的绿色可持续生产。展开更多
EMS诱变育种是作物种质创新的重要手段,利用反向遗传学手段TILLING(targeting induced local lesions in genomes)筛选EMS突变体,是研究基因功能与获得优良种质的有效手段之一。本研究采用基于HRM,即高分辨率熔解曲线分析技术的TILLING...EMS诱变育种是作物种质创新的重要手段,利用反向遗传学手段TILLING(targeting induced local lesions in genomes)筛选EMS突变体,是研究基因功能与获得优良种质的有效手段之一。本研究采用基于HRM,即高分辨率熔解曲线分析技术的TILLING筛选方法(HRM-TILLING)进行突变体筛选技术体系的探索,通过设计不同大小扩增片段引物及Mg^(2+)浓度梯度,比较了不同条件下的HRM筛选效果,结果表明当扩增片段长度为150 bp,Mg^(2+)浓度为3.0 mmol/L时,可以有效区分DNA 16倍混合池中ARF7A基因存在碱基差异的两种茄子(Solanum melongena L.)材料,建立了一套基于HRM的茄子EMS突变体TILLING技术的筛选方法。以含有2000个M2株系的茄子(EP26)EMS突变体库为材料,筛选出1个ARF7A基因和2个Pad-1基因突变体株系。本研究建立的筛选技术体系可以快速、高效地筛选茄子EMS突变体,所筛选的突变体为进一步验证、获取茄子单性结实种质及功能基因组学的研究提供研究材料。展开更多
为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,...为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,建立豌豆表型突变体库,并结合田间表型数据,筛选优异突变体材料。结果表明:(1)通过1%、8 h EMS诱变10000粒豌豆种子,M_(1)群体有4682株成苗,M_(2)群体筛选到342份豌豆突变体。(2)突变体豌豆表型性状突变类型比较丰富,其中单株籽粒干重变异系数最大,达到0.965。(3)通过对田间表型数据进行综合分析,筛选到10份优异的豌豆突变体种质。该研究结果丰富了豌豆种质资源,可为豌豆相关功能基因挖掘和研究及优良品种选育提供参考依据。展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.22475057 and No.52373262).
文摘Directional three-dimensional carbon-based foams are emerging as highly attractive candidates for promising electromagnetic wave absorbing materials(EWAMs)thanks to their unique architecture,but their construction usually involves complex procedures and extremely depends on unidirectional freezing technique.Herein,we propose a groundbreaking approach that leverages the assemblies of salting-out protein induced by ammonium metatungstate(AM)as the precursor,and then acquire directional three-dimensional carbon-based foams through simple pyrolysis.The electrostatic interaction between AM and protein ensures well dispersion of WC_(1−x)nanoparticles on carbon frameworks.The content of WC_(1−x)nanoparticles can be rationally regulated by AM dosage,and it also affects the electromagnetic(EM)properties of final carbon-based foams.The optimized foam exhibits exceptional EM absorption performance,achieving a remarkable minimum reflection loss of−72.0 dB and an effective absorption bandwidth of 6.3 GHz when EM wave propagates parallel to the directional pores.Such performance benefits from the synergistic effects of macroporous architecture and compositional design.Although there is a directional dependence of EM absorption,radar stealth simulation demonstrates that these foams can still promise considerable reduction in radar cross section with the change of incident angle.Moreover,COMSOL simulation further identifies their good performance in preventing EM interference among different electronic components.
文摘旨在探讨EM微生物菌剂与牛、羊粪配施对高海拔地区燕麦产量、品质及土壤质量的影响。以‘青海甜燕麦’为材料,比较了不同剂量的EM菌剂(EM1、EM2、EM3、EM4、EM5)与牛、羊粪配施效果,并以不施肥作为对照(CK)。在生长季节(5—10月)观察了各处理对燕麦生长发育、产量、品质、土壤养分及酶活性的影响。结果表明,施用EM菌剂显著促进了燕麦生长,尤其在株高、根总长、旗叶叶面积和鲜质量上,其中EM4处理的株高最高,较CK增加20.11%。光合作用方面,增施EM菌剂提高了燕麦SPAD值、光合速率、蒸腾速率、气孔导度和胞内CO_(2)浓度,其中EM3和EM4处理的净光合速率均超过13μmol/(m^(2)·s),较CK分别增加34.38%和33.57%。营养品质分析显示,EM3处理的粗蛋白和可溶性糖含量分别为7.13%和10.65%,较CK显著提高38.71%和32.46%,且总可消化养分含量均在60%以上,显著优于CK。在饲草产量方面,EM3处理的鲜草和干草产量最高,分别为28492.00和10928.80 kg/hm^(2),较CK分别增加28.57%和54.36%。土壤质量方面,施用EM菌剂后,土壤有机碳和全氮含量显著增加,EM3处理的全氮含量最高,较CK提高18.23%,同时土壤电导率和pH下降。土壤β-葡萄糖甘梅和N-乙酰-β-D-氨基葡萄糖苷酶活性在EM3处理达到峰值,分别较CK处理显著提高60.31%和70.68%。隶属函数综合评价分析显示,各处理对燕麦生长和土壤质量的改善效果依次为EM3>EM4>EM5>EM2>EM1>CK,表明EM3处理效果最佳。因此,在高海拔地区每公顷施用15.00 kg EM菌剂+18000 kg的牛羊粪组合效果最佳,显著促进了燕麦的生长发育,提高了其营养品质,并有效改善了土壤养分及酶活性。建议在高海拔地区推广该施肥方案,以提升燕麦的产量和土壤健康,促进高海拔地区燕麦饲草的绿色可持续生产。
文摘为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,建立豌豆表型突变体库,并结合田间表型数据,筛选优异突变体材料。结果表明:(1)通过1%、8 h EMS诱变10000粒豌豆种子,M_(1)群体有4682株成苗,M_(2)群体筛选到342份豌豆突变体。(2)突变体豌豆表型性状突变类型比较丰富,其中单株籽粒干重变异系数最大,达到0.965。(3)通过对田间表型数据进行综合分析,筛选到10份优异的豌豆突变体种质。该研究结果丰富了豌豆种质资源,可为豌豆相关功能基因挖掘和研究及优良品种选育提供参考依据。
