This study analyzed data from the DEMETER satellite,collected 10 days before the Wenchuan M_(s)8.0 earthquake on May 12,2008.The time-frequency power spectrum of the data indicated a narrow-band radiative destabilizat...This study analyzed data from the DEMETER satellite,collected 10 days before the Wenchuan M_(s)8.0 earthquake on May 12,2008.The time-frequency power spectrum of the data indicated a narrow-band radiative destabilization(below the cut-off frequency)6 days prior to the earthquake.A significant anomaly in the Ez component was observed 3 days before the event.However,similar phenomena were found over aseismic regions,suggesting a link between ionospheric changes and the earthquake generation process.展开更多
[目的]研究Netrin1调控ELF3对子宫内膜癌HEC-1B细胞转移活性的影响。[方法]将36份组织样本分为2组:子宫内膜癌组、癌旁组,每组18份。蛋白免疫印迹检测癌组织和癌旁组织中Netrin1与ELF3的表达。将子宫内膜癌HEC-1B细胞设置为3组:siRNA N...[目的]研究Netrin1调控ELF3对子宫内膜癌HEC-1B细胞转移活性的影响。[方法]将36份组织样本分为2组:子宫内膜癌组、癌旁组,每组18份。蛋白免疫印迹检测癌组织和癌旁组织中Netrin1与ELF3的表达。将子宫内膜癌HEC-1B细胞设置为3组:siRNA NC组、siRNA Netrin1组和siRNA ELF3组。MTT实验法检测HEC-1B细胞的增殖能力,细胞划痕实验方法分析HEC-1B细胞的迁移能力,TUNEL实验检测HEC-1B细胞的凋亡率,蛋白免疫印迹方法分析HEC-1B细胞中Netrin1与ELF3蛋白的表达。[结果]与癌旁组织比较,子宫内膜癌组织Netrin1与ELF3的表达上调(0.19±0.06 vs 0.73±0.02,P<0.05;0.23±0.03 vs 0.69±0.08,P<0.05)。与siRNA NC组比较,siRNA Netrin1以及siRNA ELF3组的子宫内膜癌HEC-1B细胞增殖能力下降;siRNA Netrin1以及siRNA ELF3组的HEC-1B细胞迁移能力下降;siRNA Netrin1以及siRNA ELF3组的HEC-1B细胞凋亡率增加(1.15%±0.08%vs 10.31%±0.06%vs 9.96%±0.17%,P<0.05);siRNA Netrin1组的HEC-1B细胞Netrin1、ELF3表达降低(0.78±0.06 vs 0.31±0.07 vs 0.75±0.11,P<0.05;0.73±0.08 vs 0.25±0.03 vs 0.29±0.07,P<0.05);siRNA ELF3组的HEC-1B细胞ELF3表达降低。[结论]子宫内膜癌组织中Netrin1与ELF3的表达水平增加。抑制Netrin1表达后,子宫内膜癌HEC-1B细胞的增殖与迁移能力减弱,凋亡率增加,Netrin1对子宫内膜癌的作用与调控ELF3相关。展开更多
基金National Natural Science Foundation of China(No.41274079,No.42304080)Seismic Safety and Health Monitoring of Major Construction Projects-Bridge and Reservoir Seismic Monitoring(No.DZ2024083000001)State Key Research and Development Plan under Grant(No.2018YFE0208300,No.2018YFC0603500).
文摘This study analyzed data from the DEMETER satellite,collected 10 days before the Wenchuan M_(s)8.0 earthquake on May 12,2008.The time-frequency power spectrum of the data indicated a narrow-band radiative destabilization(below the cut-off frequency)6 days prior to the earthquake.A significant anomaly in the Ez component was observed 3 days before the event.However,similar phenomena were found over aseismic regions,suggesting a link between ionospheric changes and the earthquake generation process.
文摘[目的]研究Netrin1调控ELF3对子宫内膜癌HEC-1B细胞转移活性的影响。[方法]将36份组织样本分为2组:子宫内膜癌组、癌旁组,每组18份。蛋白免疫印迹检测癌组织和癌旁组织中Netrin1与ELF3的表达。将子宫内膜癌HEC-1B细胞设置为3组:siRNA NC组、siRNA Netrin1组和siRNA ELF3组。MTT实验法检测HEC-1B细胞的增殖能力,细胞划痕实验方法分析HEC-1B细胞的迁移能力,TUNEL实验检测HEC-1B细胞的凋亡率,蛋白免疫印迹方法分析HEC-1B细胞中Netrin1与ELF3蛋白的表达。[结果]与癌旁组织比较,子宫内膜癌组织Netrin1与ELF3的表达上调(0.19±0.06 vs 0.73±0.02,P<0.05;0.23±0.03 vs 0.69±0.08,P<0.05)。与siRNA NC组比较,siRNA Netrin1以及siRNA ELF3组的子宫内膜癌HEC-1B细胞增殖能力下降;siRNA Netrin1以及siRNA ELF3组的HEC-1B细胞迁移能力下降;siRNA Netrin1以及siRNA ELF3组的HEC-1B细胞凋亡率增加(1.15%±0.08%vs 10.31%±0.06%vs 9.96%±0.17%,P<0.05);siRNA Netrin1组的HEC-1B细胞Netrin1、ELF3表达降低(0.78±0.06 vs 0.31±0.07 vs 0.75±0.11,P<0.05;0.73±0.08 vs 0.25±0.03 vs 0.29±0.07,P<0.05);siRNA ELF3组的HEC-1B细胞ELF3表达降低。[结论]子宫内膜癌组织中Netrin1与ELF3的表达水平增加。抑制Netrin1表达后,子宫内膜癌HEC-1B细胞的增殖与迁移能力减弱,凋亡率增加,Netrin1对子宫内膜癌的作用与调控ELF3相关。
