Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of th...Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of the elastic and collagen networks. In a model of collagen and elastin destruction, we showed that the gingival fibroblast was able to preserve these macromolecules by inhibiting the overproduction of metalloproteinases by overproduction of TIMP-1 and modulation of the inflammatory cytokines activity. The objective of this study is to evaluate the effect of the gingival fibroblasts on human skin. The results in vitro and ex vivo show that the gingival fibroblast protects the skin collagen and elastic network by the inhibition of MMPs which leads to an overproduction of the TIMP-1. Moreover, the gingival fibroblast modulates the activity of some enzymes responsible for the inflammation;they inhibit the IL-1β and stimulate the production of TGF-β1. In vivo studies with a duration of six months and 50 women with pronounced wrinkles show that the culture supernatant of gingival fibroblasts diluted to 5% leads to a statistically significant decrease in the number and length of wrinkles.展开更多
Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP ...Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.展开更多
Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics...Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics,however,is poorly understood.In this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left ventricles.We demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber architecture.When compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface mechanics.The myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte bundles.This collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during systole.Moreover,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in vivo.In short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.展开更多
The bifurcation regions of intracranial and extracranial arteries are common sites for atherosclerosis,predisposing to ischaemic stroke.1 Previous studies have shown that the unique haemodynamics at the bifurca-tion m...The bifurcation regions of intracranial and extracranial arteries are common sites for atherosclerosis,predisposing to ischaemic stroke.1 Previous studies have shown that the unique haemodynamics at the bifurca-tion may lead to alterations in genes and proteins in this region.2 Elastin is closely associated with the progression of athero-sclerosis.3 However,under physiological conditions,the expression and distribu-tion of elastin in bifurcation regions have not yet been elucidated.Mice are the most frequently used animal model for studying atherosclerosis.This study focuses on carotid bifurcation,optimising the iDISCO(immunolabeling-enabled three-dimensional imaging of solvent-cleared organs)technique for whole tissue clearing and staining of the carotid artery in mice.4 These techniques,along with fluorescence micro-optical sectioning tomography(FMOST)technology,have also been used in studies on ischaemic stroke and kidney diseases,4 highlighting their potential for broader applications due to their high precision and three-dimensional imaging capabilities.5 Using FMOST technology in vitro,we have made a detailed visualisation of a U-shaped expression pattern of elastin at bifurcation regions for the first time.Specifically,elastin expression is found to be lowest at the bifurcation itself compared with the regions adjacent to or proximal to the bifurcation(figures 1A,B).Further-more,we observed disorganised arrange-ment of elastic fibres within the bifurcation zone(figures 1C,D).These findings provide important evidence linking elastic fibres to the pathogenesis of atherosclerosis at bifurcations and suggest the potential for more precise local therapies for athero-sclerosis,which could significantly advance precision medicine and reduce the poten-tial side effects on normal tissues.展开更多
Despite recent advances in extrusion bioprinting of cell-laden hydrogels,using nat-urally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical...Despite recent advances in extrusion bioprinting of cell-laden hydrogels,using nat-urally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge.Here,we address this challenge with precisely designed biological tough hydrogel bioinks featuring a double-network structure.The tough hydrogels con-sisted of energy-dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid(o-nitrobenzyl-grafted hyaluronic acid)and elastin through Schiff’s base reac-tion,and free-radically polymerized gelatin methacryloyl.The incorporation of elastin further improved the elasticity,stretchability(∼170%strain),and tough-ness(∼45 kJ m-3)of the hydrogels due to the random coiling structure.We used this novel class of hydrogel bioinks to bioprint several complex elastic tissues with good shape retention.Furthermore,in vitro and in vivo experiments also demon-strated that the existence of elastin in the biocompatible bioinks facilitated improved cell behaviors and biological functions of bioprinted tissues,such as cell spreading and phenotype maintenance as well as tissue regeneration.The results confirmed the potential of the elastin-containing tough hydrogel bioinks for bioprinting of 3D complex elastic tissues with biological functionalities,which mayfind widespread applications in elastic tissue regeneration.展开更多
Background Elastin derived peptides can regulate melanocyte precursor development. Ultraviolet irradiation, infrared radiation and heat can increase the synthesis of tropoelastin in human skin epidermis. The aim of th...Background Elastin derived peptides can regulate melanocyte precursor development. Ultraviolet irradiation, infrared radiation and heat can increase the synthesis of tropoelastin in human skin epidermis. The aim of this study was to investigate whether the over expressed tropoelastin in epidermis has some role in melanogenesis of melanocytes. Methods A375 human melanoma cells were treated with different concentrations of kappa elastin for 24 hours. A375 human melanoma cells were randomly assigned to control, kappa elastin, and lactose pre-incubated groups. The cell viabilities were detected by the methyl thiazoleterazolium assay. Melanin content and tyrosinase activity in A375 melanoma cells were measured. The expressions of endothelin B receptor （ETBR） mRNA and c-kit mRNA in A375 melanoma cells were measured by quantative reverse transcription polymerase chain reaction. Results Fifty pg/ml of kappa elastin significantly increased the melanin content by 56.64% compared with the control （P 〈0.05）. Kappa elastin increased cellular tyrosinase activity by 46.73% compared with the control at 24 hours （P 〈0.05）. Kappa elastin increased the expressions of ETBR and c-kit mRNA levels by 2.13-fold and 2.47-fold compared with the controls, respectively. When pre-incubating cells with a lactose solution （10 mmol/L）, the inhibition on melanin production was 34.96% compared with the kappa elastin group （P 〈0.05）, tyrosinase activity was inhibited by 29.93% compared with kappa elastin group （P 〈0.05）, and the expressions of ETBR mRNA and c-kit mRNA were decreased by 1.56-fold and 0.82-fold compared with kappa elastin group, respectively. Conclusion Kappa elastin increased the melanogenesis in A375 melanoma cells via the stimulation of tyrosinase activity and the expression of ETBR and c-kit. The over expressed tropoelastin produced by keratinocytes might play a role in melanogenesis of epidermal melanocytes.展开更多
Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-e...Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.展开更多
目的研究肝细胞癌(hepatocellular carcinoma,HCC)组织中趋化因子受激活调节正常T细胞表达和分泌因子(regulated-upon activation normal T expressed and secreted,RANTES)和弹性蛋白(elastin)的表达水平,并探讨其临床病理意义。方法选...目的研究肝细胞癌(hepatocellular carcinoma,HCC)组织中趋化因子受激活调节正常T细胞表达和分泌因子(regulated-upon activation normal T expressed and secreted,RANTES)和弹性蛋白(elastin)的表达水平,并探讨其临床病理意义。方法选择42例手术切除的HCC癌组织和癌旁组织标本,常规石蜡包埋切片后ABC免疫组化法检测RAN-TES和elastin的表达情况。结果 42例HCC癌组织中RANTES和elastin的表达阳性率和表达评分均显著高于癌旁组织:RANTES5,4.8%vs 19.0%(P<0.01)1,.88±1.78 vs 0.69±1.28(P<0.01);elastin,45.2%vs 19.0%(P<0.05),1.64±1.85 vs 0.66±1.28(P<0.01);RANTES和elastin的表达情况与HCC的主要临床病理特征无明显关系;相关性分析显示HCC癌组织中RANTES和elastin的表达评分呈正相关(r=0.445,P<0.05)。结论 RANTES和elastin的表达与HCC的发生、发展可能关系密切;elastin与趋化因子RANTES密切相关。展开更多
文摘Skin aging shows an imbalance between synthesis and degradation of the extracellular matrix. The overproduction of degradative enzymes (MMPs) during the chronology- and photo-induced aging leads to a degradation of the elastic and collagen networks. In a model of collagen and elastin destruction, we showed that the gingival fibroblast was able to preserve these macromolecules by inhibiting the overproduction of metalloproteinases by overproduction of TIMP-1 and modulation of the inflammatory cytokines activity. The objective of this study is to evaluate the effect of the gingival fibroblasts on human skin. The results in vitro and ex vivo show that the gingival fibroblast protects the skin collagen and elastic network by the inhibition of MMPs which leads to an overproduction of the TIMP-1. Moreover, the gingival fibroblast modulates the activity of some enzymes responsible for the inflammation;they inhibit the IL-1β and stimulate the production of TGF-β1. In vivo studies with a duration of six months and 50 women with pronounced wrinkles show that the culture supernatant of gingival fibroblasts diluted to 5% leads to a statistically significant decrease in the number and length of wrinkles.
文摘Background:Collagen peptides(CP),including tripeptides and elastin peptides(EP),are known for their in vitro and in vivo anti-skin aging effects.Despite positive results in animal models,the combination effects of CP and EP and the bioavailability of CP in human studies,particularly in young and middle-aged women,remain underexplored.Objective:To evaluate the effects of an orally administered collagen drink combining CP and EP on the skin health of young and middle-aged women.Materials and Methods:A single-center,randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLABR fish collagen tripeptide beverage.Participants consumed the drink over an 8-week period.Results:Compared to the placebo group,the collagen drink group showed significant improvements in skin hydration(39.19%increase),transepidermal water loss(33.45%decrease),skin elasticity(25.37%increase),dermal collagen content(21.64%increase),pore size(7.94%decrease),wrinkle length(18.09%decrease),skin smoothness(2.85%improvement),and skin roughness(15.32%decrease).Overall pore volume decreased by 60%,and visual assessments indicated a decrease in skin luminosity by 15.20%and smoothness index by 22.55%.Mass spectrometry demonstrated a significant increase in collagen efficacy components,including blood pH and GPH levels(P<0.05).Conclusion:The study confirmed the combination nourishing and anti-skin aging effects of EP and CP on the skin of young and middle-aged women,demonstrating significant improvements in various skin parameters and good bioavailability of collagen peptides.
基金support:R01EB022018,R15HL140503,T32 HL134613 from NIH,UT STARS.
文摘Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac biomechanics.Previous studies have mainly focused on collagen,the major structural ECM in heart wall.The role of elastin in cardiac mechanics,however,is poorly understood.In this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left ventricles.We demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber architecture.When compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface mechanics.The myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte bundles.This collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during systole.Moreover,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in vivo.In short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.
基金supported by the National Natural Science Foundation of China grant number 81530037 to Yuming Xu,82401556 to Shen Li and 82301320 to Peipei Li.
文摘The bifurcation regions of intracranial and extracranial arteries are common sites for atherosclerosis,predisposing to ischaemic stroke.1 Previous studies have shown that the unique haemodynamics at the bifurca-tion may lead to alterations in genes and proteins in this region.2 Elastin is closely associated with the progression of athero-sclerosis.3 However,under physiological conditions,the expression and distribu-tion of elastin in bifurcation regions have not yet been elucidated.Mice are the most frequently used animal model for studying atherosclerosis.This study focuses on carotid bifurcation,optimising the iDISCO(immunolabeling-enabled three-dimensional imaging of solvent-cleared organs)technique for whole tissue clearing and staining of the carotid artery in mice.4 These techniques,along with fluorescence micro-optical sectioning tomography(FMOST)technology,have also been used in studies on ischaemic stroke and kidney diseases,4 highlighting their potential for broader applications due to their high precision and three-dimensional imaging capabilities.5 Using FMOST technology in vitro,we have made a detailed visualisation of a U-shaped expression pattern of elastin at bifurcation regions for the first time.Specifically,elastin expression is found to be lowest at the bifurcation itself compared with the regions adjacent to or proximal to the bifurcation(figures 1A,B).Further-more,we observed disorganised arrange-ment of elastic fibres within the bifurcation zone(figures 1C,D).These findings provide important evidence linking elastic fibres to the pathogenesis of atherosclerosis at bifurcations and suggest the potential for more precise local therapies for athero-sclerosis,which could significantly advance precision medicine and reduce the poten-tial side effects on normal tissues.
基金Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences,Grant/Award Numbers:2021-I2M-1-052,2017-I2M-1-007National Multidisciplinary Cooperative Diagnosis and Treatment Capacity Building Project for Major Diseases,Grant/Award Number:21025National Natural Science Foundation of China,Grant/Award Numbers:82371796,81871575。
文摘Despite recent advances in extrusion bioprinting of cell-laden hydrogels,using nat-urally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge.Here,we address this challenge with precisely designed biological tough hydrogel bioinks featuring a double-network structure.The tough hydrogels con-sisted of energy-dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid(o-nitrobenzyl-grafted hyaluronic acid)and elastin through Schiff’s base reac-tion,and free-radically polymerized gelatin methacryloyl.The incorporation of elastin further improved the elasticity,stretchability(∼170%strain),and tough-ness(∼45 kJ m-3)of the hydrogels due to the random coiling structure.We used this novel class of hydrogel bioinks to bioprint several complex elastic tissues with good shape retention.Furthermore,in vitro and in vivo experiments also demon-strated that the existence of elastin in the biocompatible bioinks facilitated improved cell behaviors and biological functions of bioprinted tissues,such as cell spreading and phenotype maintenance as well as tissue regeneration.The results confirmed the potential of the elastin-containing tough hydrogel bioinks for bioprinting of 3D complex elastic tissues with biological functionalities,which mayfind widespread applications in elastic tissue regeneration.
基金This study was supported by a grant from the National Natural Science Foundation of China
文摘Background Elastin derived peptides can regulate melanocyte precursor development. Ultraviolet irradiation, infrared radiation and heat can increase the synthesis of tropoelastin in human skin epidermis. The aim of this study was to investigate whether the over expressed tropoelastin in epidermis has some role in melanogenesis of melanocytes. Methods A375 human melanoma cells were treated with different concentrations of kappa elastin for 24 hours. A375 human melanoma cells were randomly assigned to control, kappa elastin, and lactose pre-incubated groups. The cell viabilities were detected by the methyl thiazoleterazolium assay. Melanin content and tyrosinase activity in A375 melanoma cells were measured. The expressions of endothelin B receptor （ETBR） mRNA and c-kit mRNA in A375 melanoma cells were measured by quantative reverse transcription polymerase chain reaction. Results Fifty pg/ml of kappa elastin significantly increased the melanin content by 56.64% compared with the control （P 〈0.05）. Kappa elastin increased cellular tyrosinase activity by 46.73% compared with the control at 24 hours （P 〈0.05）. Kappa elastin increased the expressions of ETBR and c-kit mRNA levels by 2.13-fold and 2.47-fold compared with the controls, respectively. When pre-incubating cells with a lactose solution （10 mmol/L）, the inhibition on melanin production was 34.96% compared with the kappa elastin group （P 〈0.05）, tyrosinase activity was inhibited by 29.93% compared with kappa elastin group （P 〈0.05）, and the expressions of ETBR mRNA and c-kit mRNA were decreased by 1.56-fold and 0.82-fold compared with kappa elastin group, respectively. Conclusion Kappa elastin increased the melanogenesis in A375 melanoma cells via the stimulation of tyrosinase activity and the expression of ETBR and c-kit. The over expressed tropoelastin produced by keratinocytes might play a role in melanogenesis of epidermal melanocytes.
基金sponsored by the National Key R&D Program of China(2018YFD0901102)the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(ZS20190105)+2 种基金the Natural Foundation of Zhejiang Province(LQ22D060002)the Cooperative Project of Vigour Health Products Co.,Ltd,Shenzhenthe K.C.Wong Magna Fund of Ningbo University.
文摘Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.
文摘目的研究肝细胞癌(hepatocellular carcinoma,HCC)组织中趋化因子受激活调节正常T细胞表达和分泌因子(regulated-upon activation normal T expressed and secreted,RANTES)和弹性蛋白(elastin)的表达水平,并探讨其临床病理意义。方法选择42例手术切除的HCC癌组织和癌旁组织标本,常规石蜡包埋切片后ABC免疫组化法检测RAN-TES和elastin的表达情况。结果 42例HCC癌组织中RANTES和elastin的表达阳性率和表达评分均显著高于癌旁组织:RANTES5,4.8%vs 19.0%(P<0.01)1,.88±1.78 vs 0.69±1.28(P<0.01);elastin,45.2%vs 19.0%(P<0.05),1.64±1.85 vs 0.66±1.28(P<0.01);RANTES和elastin的表达情况与HCC的主要临床病理特征无明显关系;相关性分析显示HCC癌组织中RANTES和elastin的表达评分呈正相关(r=0.445,P<0.05)。结论 RANTES和elastin的表达与HCC的发生、发展可能关系密切;elastin与趋化因子RANTES密切相关。
