EGL3(Enhancer of Glabra3)蛋白是一种bHLH类蛋白,是一类重要的转录调节因子。为阐释BrEGL3在‘津田’芜菁(Brassica campestris L.ssp.rapifera‘Tsuda’)花青素生物合成中的调控作用,根据拟南芥EGL3基因序列信息设计兼并引物,克隆获得...EGL3(Enhancer of Glabra3)蛋白是一种bHLH类蛋白,是一类重要的转录调节因子。为阐释BrEGL3在‘津田’芜菁(Brassica campestris L.ssp.rapifera‘Tsuda’)花青素生物合成中的调控作用,根据拟南芥EGL3基因序列信息设计兼并引物,克隆获得了'津田'芜菁EGL3基因的全长cDNA序列,命名为BrEGL3,其GenBank登录号为HM208589。序列分析结果显示,BrEGL3全长1794bp,包含编码597个氨基酸的开放阅读框,分子量约为66.8kD。氨基酸结构分析显示该蛋白含有DNA结合域,该结合属于螺旋-环-螺旋家族。氨基酸对比分析表明,BrEGL3与萝卜EGL3蛋白相似性最高,其次为拟南芥中的EGL3。荧光定量PCR检测BrEGL3在芜菁不同组织中的表达结果表明,该基因在有花青素合成的红色芜菁根皮中表达量最高。展开更多
In electroplating lines,many conductor rolls are installed in electroplating bath.Typical electroplatings are tin plating(ETL) and zinc plating(EGL),and from required product qualities,a vertical cell for the former a...In electroplating lines,many conductor rolls are installed in electroplating bath.Typical electroplatings are tin plating(ETL) and zinc plating(EGL),and from required product qualities,a vertical cell for the former and a horizontal cell for the latter is often used.Generally,chrome plating or WC cermet thermal spray coating is applied to stainless steel conductor roll in ETL for prolonging service life by improvement of wear resistance and corrosion resistance.On the other hand,Hastelloy type alloy substrate is used for conductor rolls in EGL due to severe corrosion environment of electro plating solution.Thermal spray coating is effective for reducing wear,but there are many cases where the coating cannot complete expected service life when corrosion becomes an issue.In this study,thermal spray coating for ETL conductor roll and development of the coating for EGL conductor roll to meet expected service life are described.展开更多
文摘目的探讨Egl-9家族缺氧诱导因子1(Egl-9 family hypoxia inducible factor 1,EGLN1)缺失对结直肠癌(colorectal cancer,CRC)细胞增殖、迁移、侵袭及自噬通路的影响,为CRC精准治疗提供潜在靶点。方法利用CRISPR-Cas9技术构建EGLN1敲除的LoVo与RKO细胞株;通过CCK-8法、集落形成、Transwell实验及划痕愈合实验,分别检测细胞的增殖、侵袭及迁移能力。Western blot分析自噬标志物LC3B-Ⅱ/LC3B-Ⅰ值与p62蛋白表达水平,评估自噬通路激活情况。通过建立裸鼠皮下移植瘤模型,评估自噬激活剂雷帕霉素对EGLN1敲除异种移植瘤生长的影响。采用苏木精-伊红(hematoxylin and eosin,HE)染色和Ki-67免疫组化检测异种移植瘤组织细胞增殖情况。结果成功构建稳定的EGLN1敲除细胞系。EGLN1的缺失显著增强LoVo和RKO细胞增殖、迁移和侵袭(均P<0.05);自噬相关蛋白分析结果显示,EGLN1敲除后LC3B-Ⅱ/LC3B-Ⅰ值下降,p62显著积累。采用晚期自噬抑制剂氯喹或早期抑制剂3-甲基腺嘌呤处理进一步加剧自噬抑制。体内实验表明,EGLN1敲除组的肿瘤体积显著大于EGLN1野生型组(P<0.05),而雷帕霉素治疗有效抑制了肿瘤生长。HE染色和Ki-67免疫组化证实,EGLN1敲除促进异种移植瘤中的细胞增殖,而雷帕霉素可逆转该促瘤效应。结论EGLN1通过激活自噬通路抑制CRC细胞的恶性表型,其缺失可能导致自噬抑制,从而促进肿瘤细胞增殖、迁移与侵袭。靶向EGLN1/自噬轴可能是治疗CRC的有效策略。
文摘EGL3(Enhancer of Glabra3)蛋白是一种bHLH类蛋白,是一类重要的转录调节因子。为阐释BrEGL3在‘津田’芜菁(Brassica campestris L.ssp.rapifera‘Tsuda’)花青素生物合成中的调控作用,根据拟南芥EGL3基因序列信息设计兼并引物,克隆获得了'津田'芜菁EGL3基因的全长cDNA序列,命名为BrEGL3,其GenBank登录号为HM208589。序列分析结果显示,BrEGL3全长1794bp,包含编码597个氨基酸的开放阅读框,分子量约为66.8kD。氨基酸结构分析显示该蛋白含有DNA结合域,该结合属于螺旋-环-螺旋家族。氨基酸对比分析表明,BrEGL3与萝卜EGL3蛋白相似性最高,其次为拟南芥中的EGL3。荧光定量PCR检测BrEGL3在芜菁不同组织中的表达结果表明,该基因在有花青素合成的红色芜菁根皮中表达量最高。
文摘In electroplating lines,many conductor rolls are installed in electroplating bath.Typical electroplatings are tin plating(ETL) and zinc plating(EGL),and from required product qualities,a vertical cell for the former and a horizontal cell for the latter is often used.Generally,chrome plating or WC cermet thermal spray coating is applied to stainless steel conductor roll in ETL for prolonging service life by improvement of wear resistance and corrosion resistance.On the other hand,Hastelloy type alloy substrate is used for conductor rolls in EGL due to severe corrosion environment of electro plating solution.Thermal spray coating is effective for reducing wear,but there are many cases where the coating cannot complete expected service life when corrosion becomes an issue.In this study,thermal spray coating for ETL conductor roll and development of the coating for EGL conductor roll to meet expected service life are described.
