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鞘氨醇激酶促进胃癌细胞SGC-7901转移和侵袭 被引量:5
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作者 史福军 黄宗海 +4 位作者 厉周 蔡寨 李秀勤 彭亮 刘灏 《第三军医大学学报》 CAS CSCD 北大核心 2014年第1期25-28,共4页
目的探讨鞘氨醇激酶-1(sphingosine kinase 1,SphK1)促进胃癌细胞转移和侵袭的机制。方法使用转染、RNA干扰及Sphk1过表达载体构建的方法分别实现胃癌细胞SGC-7901中SphK1蛋白的过表达、低表达,通过Transwell实验检测SphK1对胃癌细胞转... 目的探讨鞘氨醇激酶-1(sphingosine kinase 1,SphK1)促进胃癌细胞转移和侵袭的机制。方法使用转染、RNA干扰及Sphk1过表达载体构建的方法分别实现胃癌细胞SGC-7901中SphK1蛋白的过表达、低表达,通过Transwell实验检测SphK1对胃癌细胞转移和侵袭能力的影响,并利用ELISA检测1-磷酸鞘氨醇(sphingosine 1-phosphate,S1P)及其受体水平的变化。结果 SphK1的过表达可显著促进胃癌细胞的转移和侵袭(P<0.05),而SphK1特异性siRNA可轻微降低胃癌细胞的转移和侵袭能力,但与对照相比差异不显著。SphK1转染细胞S1P表达水平(0.32±0.01)高于对照组(0.24±0.02)(P<0.05),SphK1的过表达可提高S1P的水平,而外源性S1P的添加可通过EDG1促进胃癌细胞的转移和侵袭。结论 SphK1/S1P/EDG1途径在胃癌的转移中有重要的作用,为胃癌的诊断及治疗提供了一个可能的新靶点。 展开更多
关键词 鞘氨醇激酶 1-磷酸鞘氨醇 edg1 胃癌细胞
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No replicated association of the c.-312A >G in<i>EDG</i>1 with marbling in Niigata population of Japanese Black beef cattle
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作者 Bin Tong Narumi Fuke +5 位作者 Yui Himizu Hiroyuki Katou Masato Hatano Takeshi Ohta Hiroyuki Kose Takahisa Yamada 《Open Journal of Animal Sciences》 2013年第4期269-272,共4页
Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucle... Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucleotide polymorphism (SNP), namely, c.-312A > G, in the endothelial differentiation sphingolipid G-proteincoupled receptor, 1 (EDG1) gene was associated with the BMS level in the Japanese Black beef cattle population of Oita prefecture, with the G allele being associated with a high level of the BMS. Thus, the c.-312A > G SNP seems to be a candidate marker for marker-assisted selection. In this study, we investigated whether this association could be replicated in the Japanese Black beef cattle population of Niigata prefecture and analyzed the effect of the SNP genotypes on the carcass traits other than the BMS. No significant differences in the BMS level were detected among the genotypes of the c.-312A > G SNP in the Niigata Japanese Black beef cattle population. The SNP genotype had no significant effects on the carcass weight, rib eye area and rib thickness of the cattle population. These findings suggested that the association of the c.-312A > G SNP with the BMS level in the Japanese Black beef cattle population was not replicated in the Niigata population, and revealed no effects of the SNP genotype on the beef productivity in the Niigata population. Thus, we concluded that the c.-312A > G SNP is not useful for effective marker-assisted selection to increase meat quality and, additionally, meat productivity in Japanese Black beef cattle of Niigata prefecture. 展开更多
关键词 ASSOCIATION edg1 Japanese Black BREED MARBLING Replication Study Single NUCLEOTIDE Polymorphism
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Edge Structure of Reynolds Stress and Poloidal Flow on the HL-1M Tokamak 被引量:6
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作者 洪文玉 王恩耀 +2 位作者 曹建勇 李强 刘达致 《Plasma Science and Technology》 SCIE EI CAS CSCD 2001年第3期791-796,共6页
The measurement on radial profile of electrostatic Reynolds stress, plasma poloidal rotations, radial and poloidal electric field have been performed in the plasma boundary region of the HL-IM Tokamak using a multi-ar... The measurement on radial profile of electrostatic Reynolds stress, plasma poloidal rotations, radial and poloidal electric field have been performed in the plasma boundary region of the HL-IM Tokamak using a multi-array of Mach/Langmuir probes. In the experiments of Lower Hybrid Current Drive (LHCD), Supersonic Molecular Beam injection (SMBI), Multi-shot Pellet Injection (MPI) and Neutral Beam injection (NBI), the correlation between the Reynolds stress and poloidal flow in the edge plasma is presented. The results indicate that a sheared poloidal flow can be generated in Tokamak plasma due to radially varying Reynolds stress. 展开更多
关键词 LHCD Edge Structure of Reynolds Stress and Poloidal Flow on the HL-1M Tokamak HL
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Experiments and Studies of Edge Plasma Radial Transport and Confinement Property on the HL-1M Tokamak 被引量:1
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作者 洪文玉 王恩耀 +1 位作者 潘宇东 李强 《Plasma Science and Technology》 SCIE EI CAS CSCD 2000年第4期347-356,共10页
This paper describes a Mach/Langmuir probe array with five pins and six pins, which can measure not only parallel flows and the flow perpendicular to the magnetic field but also the radial and the poloidal electric fi... This paper describes a Mach/Langmuir probe array with five pins and six pins, which can measure not only parallel flows and the flow perpendicular to the magnetic field but also the radial and the poloidal electric field E. arid E as well. Experimental measurements of the edge fluctuations, velocities of the toroidal, the poloidal flow and electric field have been carried out on both of SOL and the boundary region of HL-1M for Ohmic, biased H-mode, Lower Hybrid Current Drive (LHCD), Supersonic Molecular Beam Injection (MBI), Multi-shot Pellet Injection (MPI), Neutral Beam Injection (NBI), Ion Cyclotron Resonance Heating (ICRH) and Electric Cyclotron Resonance Heating (ECRH) discharges. The results show that the suppressions of the fluctuations are related to poloidal rotations produced by different discharge modes in the improved particle confinement property, simultaneously the change of the radial and poloidal electric field is generated and becomes more negative at the Tokamak plasma edge, and the sheared poloidal flow is related to the reduction in fluctuation level, and the poloidal velocity is mainly dominated by the E × B drift. 展开更多
关键词 LHCD Experiments and Studies of Edge Plasma Radial Transport and Confinement Property on the HL-1M Tokamak ICRH ECRH HL
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The Applications of Generalized Computing in Decision-making 被引量:3
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作者 YAO Min, HUANG Yan-jun 1. Dopt. of Computer, Zhejiang University, Hangzhou 3l0028, China Key Laboratory of Education Mnistry for Image Processing and Intelligent Control 2. School of Economics, Zhejianig University, Hangzhou 310028, China 《Journal of Systems Science and Systems Engineering》 SCIE EI CSCD 2001年第4期397-402,共6页
Knowledge is one of the most important elements in production and the first resource in enterprise. By introducing knowledge to decision making, this paper presents a kind of know1edge decision- making model (KDM for ... Knowledge is one of the most important elements in production and the first resource in enterprise. By introducing knowledge to decision making, this paper presents a kind of know1edge decision- making model (KDM for short) based on generalized computing. 展开更多
关键词 know1edge DECISION-MAKING generalized computing
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Edge engineering in chemically active two-dimensional materials
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作者 Lijun Zhou Mengyan Li +3 位作者 Wei Wang Cong Wang Huiping Yang Yang Cao 《Nano Research》 SCIE EI CSCD 2022年第11期9890-9905,共16页
When "cut off" continuous and uniform basal plane of two-dimensional(2D)materials,edges appear at cross-sections.Such edges with unique one-dimensional(1D)structures and bound-states significantly alter mate... When "cut off" continuous and uniform basal plane of two-dimensional(2D)materials,edges appear at cross-sections.Such edges with unique one-dimensional(1D)structures and bound-states significantly alter materials’local chemical activities and have been extensively investigated as model platforms for investigating structure–property–performance relationships for chemistry.Many interesting phenomena have been discovered in the past decades,highlighting the importance of interactions between active species and edge atoms at the atomic level and making 1D edges as emerging catalysts with high efficiency,promising candidates for battery and electrochemical contacts.Here,this review focuses on the recent progress of edge synthesis and structural engineering methods,understanding of edge structure–activity mechanisms,and potential applications using edge sites.Challenges and prospects are also envisioned. 展开更多
关键词 two-dimensional(2D)materials one-dimensional(1D)edges relation of structure-activity
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