Echovirus 18(E18), a serotype of Enterovirus B(EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in China's Mainland, but became the predominant pathogen associated with v...Echovirus 18(E18), a serotype of Enterovirus B(EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in China's Mainland, but became the predominant pathogen associated with viral encephalitis(VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in China's Mainland, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease(HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from China's Mainland after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 50-untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiplerecombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics.展开更多
Objective:To identify the circulating serotypes of human echovinis in Malaysia from 2002 in 2013.Methods:A toial of 31 retrospective samples from non-polio acute flacid paralysis,hand-food-and-mouth disease,viral meni...Objective:To identify the circulating serotypes of human echovinis in Malaysia from 2002 in 2013.Methods:A toial of 31 retrospective samples from non-polio acute flacid paralysis,hand-food-and-mouth disease,viral meningitis and enterovirus cases were subjected to amplification of partial VPI gene by RT-PCR.Results:Sequencing and phylogeneiic analysis of the partial sequences identified presence of human echovinis and human coxsackie viruses.It was found that echovinis 11 was the commonly circulating serotype followed by echovinis6.echovinis 7.echovinis 3.echovinis 9.echovinis 30 and echovinis I in decreasing order.Additionally two types of human coxsackie virus isolates were detected which were coxsackie A24 and B3.Condusions:From the findings,there is a possibility that echovinis 11 is the predominant serotype among Malaysian patients with echovinis infection.However,a larger sample size will yield a more confident result to support this evidence.____________________展开更多
The objective of this study was to investigate the outbreak of echovirus 11 (ECHO 11) infection in newborns at a hospital in Guangdong Province, China, and to study the effectiveness of prevention and control measures...The objective of this study was to investigate the outbreak of echovirus 11 (ECHO 11) infection in newborns at a hospital in Guangdong Province, China, and to study the effectiveness of prevention and control measures to infer the epidemiological characteristics of ECHO 11 and explore the effective measures for its prevention and control. We performed retrospective analyses of hospital records and laboratory test data. In this outbreak, ten cases of ECHO 11 infection were identified, of which nine cases were nosocomial infections. Most of the cases (90%) were severe, and three died. The onset time interval of 10 patients was 1-4 days, most of which occur in 1-2 days. There were eight (80%) males and two females (20%). The gestational age of the patients was 31 to 40 weeks (mean, 35+4 weeks;median, 35+4 weeks). The onset time was 3-26 days (average 9 days;median 8 days). The birth weights of the patients ranged from 1,650 g to 3,450 g (mean 2,385 g;median 2,250 g). We concluded that neonatal infection with ECHO 11 will lead to serious symptoms and high mortality, and is prone to outbreaks of nosocomial infection. We speculate that ECHO 11 is most likely to spread via contact transmission;however, we do not rule out the possibility of droplet transmission. Prevention and control measures can effectively prevent and control hospital enteroviral infections.展开更多
In October 2017,a small outbreak of echovirus 30(E30)associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region.That year,we observed a significant increase in E...In October 2017,a small outbreak of echovirus 30(E30)associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region.That year,we observed a significant increase in E30 levels in an acute flaccid paralysis(AFP)case surveillance system.To investigate their phylogenetic relationships,we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases,AFP cases,and healthy children.We found that the E30 strains circulating in Ningxia belong to two lineages(H and J).The strains isolated in 2010,2012,and 2016 belonged to the H lineage.In 2017,a new lineage,J,emerged as the dominant lineage.Phylogenetic trees were constructed based on the whole genome andP1,P2,andP3 regions;clustering with other types of enterovirus species B was found,suggesting that recombination events had occurred.The recombination sites were mainly in regions2B,2C,and3D.This study confirmed that the E30 strains in Ningxia in 2010,2012,and 2016 had different recombination patterns and were recombined with different enteroviruses.The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia.展开更多
Background:Although aseptic meningitis associated with echovirus type 30 has emerged as a global public health concern,no data have been reported on children's immune status against echovirus type 30.The current s...Background:Although aseptic meningitis associated with echovirus type 30 has emerged as a global public health concern,no data have been reported on children's immune status against echovirus type 30.The current study aimed to investigate the seropositivity among Korean children for antibodies against echovirus 30.Methods:Two hundred and fifty residual serum samples were collected at St.Paul's Hospital.Individuals were categorized by age into four groups:group 1 (3 months-2 years),group 2 (3-6 years),group 3 (7-10 years) and group 4 (11-15 years).Neutralizing antibodies against echovirus 30 were measured.Results:Seroprotective neutralizing antibodies against echovirus 30 were detected in 129 (49%) individuals.Seropositivity rates were 23%,48%,55% and 73% in groups 1-4,respectively.For antibody titers,1:256-1:512 was the highest neutralizing antibody titer range in group 2,while 1:1024-1:2048 in group 3 and 4.Among the seropositive individuals in group 3 and 4,6% and 12% had neutralizing antibody titers of 1:2048,respectively.Conclusions:The seropositivity rate increased significantly with age..The distribution of neutralizing antibody titers varied by age group,and higher ranges of neutralizing antibody titers were observed in higher age groups.These findings suggest high susceptibility to echovirus 30 infection in children younger than 2 years old.Echovirus 30 infection in childhood may have contributed to increased neutralizing antibody titers with age.展开更多
基金supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant Nos. 2017ZX10104001-005-010 and 2017ZX10103004-004)Beijing Natural Science Foundation (Grant No. 7184208)+2 种基金Capital Clinical Feature Project of Beijing Technology Program (Grant No. Z151100004015046)Basic and Clinical Research Cooperation Project of Capital Medical University (Grant No. 17JL11)Research Training Fund of Capital Medical University (Grant No. PYZ2017012)
文摘Echovirus 18(E18), a serotype of Enterovirus B(EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in China's Mainland, but became the predominant pathogen associated with viral encephalitis(VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in China's Mainland, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease(HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from China's Mainland after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 50-untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiplerecombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics.
基金supported by Institute for Medical Research Operational Grant(Vol:040300/OS27403)
文摘Objective:To identify the circulating serotypes of human echovinis in Malaysia from 2002 in 2013.Methods:A toial of 31 retrospective samples from non-polio acute flacid paralysis,hand-food-and-mouth disease,viral meningitis and enterovirus cases were subjected to amplification of partial VPI gene by RT-PCR.Results:Sequencing and phylogeneiic analysis of the partial sequences identified presence of human echovinis and human coxsackie viruses.It was found that echovinis 11 was the commonly circulating serotype followed by echovinis6.echovinis 7.echovinis 3.echovinis 9.echovinis 30 and echovinis I in decreasing order.Additionally two types of human coxsackie virus isolates were detected which were coxsackie A24 and B3.Condusions:From the findings,there is a possibility that echovinis 11 is the predominant serotype among Malaysian patients with echovinis infection.However,a larger sample size will yield a more confident result to support this evidence.____________________
基金financially supported by the National Natural Science Foundation of China(No.71704031).
文摘The objective of this study was to investigate the outbreak of echovirus 11 (ECHO 11) infection in newborns at a hospital in Guangdong Province, China, and to study the effectiveness of prevention and control measures to infer the epidemiological characteristics of ECHO 11 and explore the effective measures for its prevention and control. We performed retrospective analyses of hospital records and laboratory test data. In this outbreak, ten cases of ECHO 11 infection were identified, of which nine cases were nosocomial infections. Most of the cases (90%) were severe, and three died. The onset time interval of 10 patients was 1-4 days, most of which occur in 1-2 days. There were eight (80%) males and two females (20%). The gestational age of the patients was 31 to 40 weeks (mean, 35+4 weeks;median, 35+4 weeks). The onset time was 3-26 days (average 9 days;median 8 days). The birth weights of the patients ranged from 1,650 g to 3,450 g (mean 2,385 g;median 2,250 g). We concluded that neonatal infection with ECHO 11 will lead to serious symptoms and high mortality, and is prone to outbreaks of nosocomial infection. We speculate that ECHO 11 is most likely to spread via contact transmission;however, we do not rule out the possibility of droplet transmission. Prevention and control measures can effectively prevent and control hospital enteroviral infections.
基金supported by the Science and Natural Science of Ningxia (Grant No.2021AAC03415)the Science and Natural Science of China (Grant No.81960607)+1 种基金Science and Natural Science of Ningxia (Grant No.2022AAC03708)the National Key Research and Development Program of China (Project No.2021YFC2302003).
文摘In October 2017,a small outbreak of echovirus 30(E30)associated with aseptic meningitis in nine cases occurred at a primary school in the Ningxia Hui Autonomous Region.That year,we observed a significant increase in E30 levels in an acute flaccid paralysis(AFP)case surveillance system.To investigate their phylogenetic relationships,we determined the whole genomic sequences of 12 strains isolated from aseptic meningitis cases,AFP cases,and healthy children.We found that the E30 strains circulating in Ningxia belong to two lineages(H and J).The strains isolated in 2010,2012,and 2016 belonged to the H lineage.In 2017,a new lineage,J,emerged as the dominant lineage.Phylogenetic trees were constructed based on the whole genome andP1,P2,andP3 regions;clustering with other types of enterovirus species B was found,suggesting that recombination events had occurred.The recombination sites were mainly in regions2B,2C,and3D.This study confirmed that the E30 strains in Ningxia in 2010,2012,and 2016 had different recombination patterns and were recombined with different enteroviruses.The 2017 epidemic E30 originated from another new lineage with a complex recombination pattern and formed an independent transmission chain in Ningxia.
文摘Background:Although aseptic meningitis associated with echovirus type 30 has emerged as a global public health concern,no data have been reported on children's immune status against echovirus type 30.The current study aimed to investigate the seropositivity among Korean children for antibodies against echovirus 30.Methods:Two hundred and fifty residual serum samples were collected at St.Paul's Hospital.Individuals were categorized by age into four groups:group 1 (3 months-2 years),group 2 (3-6 years),group 3 (7-10 years) and group 4 (11-15 years).Neutralizing antibodies against echovirus 30 were measured.Results:Seroprotective neutralizing antibodies against echovirus 30 were detected in 129 (49%) individuals.Seropositivity rates were 23%,48%,55% and 73% in groups 1-4,respectively.For antibody titers,1:256-1:512 was the highest neutralizing antibody titer range in group 2,while 1:1024-1:2048 in group 3 and 4.Among the seropositive individuals in group 3 and 4,6% and 12% had neutralizing antibody titers of 1:2048,respectively.Conclusions:The seropositivity rate increased significantly with age..The distribution of neutralizing antibody titers varied by age group,and higher ranges of neutralizing antibody titers were observed in higher age groups.These findings suggest high susceptibility to echovirus 30 infection in children younger than 2 years old.Echovirus 30 infection in childhood may have contributed to increased neutralizing antibody titers with age.
