Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paraca...Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paracarcinoma and 15 patients with liver cirrhosis were detected by S-P immunohistochemical staining. Results: The expression of E2F in hepatic carcinoma was significantly higher than that in paracarcinoma or liver cirrhosis (P<0.005), the expression of Bc1-2 in hepatic carcinoma was significantly higher than that in paracarcinoma (P<0.005), in which Bc1-2 expression was lower than in liver cirrhosis(P<0.05). The expression of E2F-3 was related with histological grade, tumor size, and the expression of Bc1-2 was related with histological grade, tumor size and tumor number. There was correlation between the expression of E2F-3 and Bc1-2 in hepatic carcinoma. Conclusion: E2F-3 and Bc1-2 expression may play an important role in development, progression and cell apoptosis of tumor.展开更多
文摘目的探讨细胞周期调控因子E2F-3和pRb蛋白在前列腺癌发病机制中的作用及其临床意义。方法应用免疫组化E liV isionTMp lus二步法检测49例前列腺癌(prostate cancer,PCa)标本,20例良性前列腺增生(ben ign prostatichyperp lasia,BPH)及10例正常前列腺组织(norm al prostate,NP)中E2F-3与pRb蛋白的表达。结果E2F-3在PCa、BPH与NP中的阳性表达率为63.27%(31/49),20%(4/20)和10%(1/10),PCa中E2F-3的水平明显高于BPH(P<0.05)及NP(P<0.05),且与PCa病理分级临床分期呈正相关(rs分别为0.487、0.517,P<0.05)。pRb中在PCa,BPH与NP中的阳性表达率为44.90%(22/49),80%(16/20),90%(9/10),PCa中pRb的水平明显低于BPH及NP(P<0.05),且与PCa病理分级、临床分期呈负相关(rs分别为-0.401、-0.468,P<0.05)。E2F-3表达与pRb表达呈负相关(rs=-0.617,P<0.01)。结论E2F-3对PCa的发生发展发挥有重要的作用,且其表达与pRb蛋白的异常表达密切相关。
文摘Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paracarcinoma and 15 patients with liver cirrhosis were detected by S-P immunohistochemical staining. Results: The expression of E2F in hepatic carcinoma was significantly higher than that in paracarcinoma or liver cirrhosis (P<0.005), the expression of Bc1-2 in hepatic carcinoma was significantly higher than that in paracarcinoma (P<0.005), in which Bc1-2 expression was lower than in liver cirrhosis(P<0.05). The expression of E2F-3 was related with histological grade, tumor size, and the expression of Bc1-2 was related with histological grade, tumor size and tumor number. There was correlation between the expression of E2F-3 and Bc1-2 in hepatic carcinoma. Conclusion: E2F-3 and Bc1-2 expression may play an important role in development, progression and cell apoptosis of tumor.
