A soft X-ray energy materials research beamline(BL20U2),a branch of energy materials beamline(E-line),has been constructed in the Shanghai Synchrotron Radiation Facility(SSRF)Phase-Ⅱ project.It is now operational for...A soft X-ray energy materials research beamline(BL20U2),a branch of energy materials beamline(E-line),has been constructed in the Shanghai Synchrotron Radiation Facility(SSRF)Phase-Ⅱ project.It is now operational for soft X-ray resonant emission spectroscopy(RXES)and soft X-ray resonant elastic scattering(REXS)investigations.Optical optimization was implemented for high performance,e.g.,photon flux,energy-resolving power,and focus spot size.RXES experiments show that the energy range extends from 150 to 1500 eV.The elastic peak measured near titanium absorption edge(@445 eV)indicates an energy resolution of the RXES spectrometer of 65 meV.The measured photon flux is 3×10^(12)photons/s at 244 eV at the RXES sample position for an SSRF electron energy of 3.5 GeV and a projected ring current as 300 mA.The spot size at the RXES sample position is 23μm in the horizontal direction and 7.9μm in the vertical direction,respectively.Moreover,the angular resolution of elastic REXS scatterometer reaches 0.005°through measurement of X-ray reflection from the single-crystal silicon wafers.A sample of the REXS scatterometer is vibrationally decoupled from its chamber and cooled using copper braids connected from an open cycle liquid helium cryo reservoir,whereas the minimum sample temperature is below 15 K.展开更多
We report the design of a wide-range energy material beamline(E-line) with multiple experimental techniques at the Shanghai Synchrotron Radiation Facility.The undulators consisted of an elliptically polarizing undulat...We report the design of a wide-range energy material beamline(E-line) with multiple experimental techniques at the Shanghai Synchrotron Radiation Facility.The undulators consisted of an elliptically polarizing undulator and in-vacuum undulator that generate the soft and hard X-rays, respectively. The beamline covered a wide energy range from 130 to 18 ke V with both a high photon flux([ 10^(12) phs/s with exit silt 30 lm in soft X-ray and [ 5 9 10^(12) phs/s in hard X-ray within 0.1%BW bandwidth) and promising resolving power(maximum E/DE [ 15,000 in soft X-ray with exit silt 30 lm and [6000 in hard X-ray). Moreover, the beam spots from the soft and hard X-rays were focused to the same sample position with a high overlap ratio, so that the surfaces, interfaces, and bulk properties were characterized in situ by changing the probing depth.展开更多
目的探讨上皮性细胞粘附分子(E-cadherin,E-cad)在人卵巢浆液性囊腺癌高低转移细胞株HO-8910PM及HO-8910中的表达及生物学行为的差异。方法采用免疫荧光法及W estern b lotting检测细胞中E-cad蛋白表达的差异,用粘附实验检测细胞与细胞...目的探讨上皮性细胞粘附分子(E-cadherin,E-cad)在人卵巢浆液性囊腺癌高低转移细胞株HO-8910PM及HO-8910中的表达及生物学行为的差异。方法采用免疫荧光法及W estern b lotting检测细胞中E-cad蛋白表达的差异,用粘附实验检测细胞与细胞外基质的粘附能力,用Transwell小室法检测细胞侵袭能力及迁移能力。结果E-cad在HO-8910细胞中为高表达,在HO-8910PM细胞中为低表达,HO-8910PM细胞与细胞外基质的粘附能力、侵袭能力及迁移能力均明显高于HO-8910细胞(P<0.05,P<0.01)。结论人卵巢浆液性曩腺癌HO-8910PM细胞的浸润、转移等恶性行为可能与E-cad的表达下降有关。展开更多
文摘A soft X-ray energy materials research beamline(BL20U2),a branch of energy materials beamline(E-line),has been constructed in the Shanghai Synchrotron Radiation Facility(SSRF)Phase-Ⅱ project.It is now operational for soft X-ray resonant emission spectroscopy(RXES)and soft X-ray resonant elastic scattering(REXS)investigations.Optical optimization was implemented for high performance,e.g.,photon flux,energy-resolving power,and focus spot size.RXES experiments show that the energy range extends from 150 to 1500 eV.The elastic peak measured near titanium absorption edge(@445 eV)indicates an energy resolution of the RXES spectrometer of 65 meV.The measured photon flux is 3×10^(12)photons/s at 244 eV at the RXES sample position for an SSRF electron energy of 3.5 GeV and a projected ring current as 300 mA.The spot size at the RXES sample position is 23μm in the horizontal direction and 7.9μm in the vertical direction,respectively.Moreover,the angular resolution of elastic REXS scatterometer reaches 0.005°through measurement of X-ray reflection from the single-crystal silicon wafers.A sample of the REXS scatterometer is vibrationally decoupled from its chamber and cooled using copper braids connected from an open cycle liquid helium cryo reservoir,whereas the minimum sample temperature is below 15 K.
基金supported by the National Development and Reform Commission(NDRC) of Chinathe National Natural Science Foundation of China(No.11505280)+1 种基金the Shanghai Youth Foundation(No.14YF1407500)the National Science Foundation of China(Nos.11475251,11225527)
文摘We report the design of a wide-range energy material beamline(E-line) with multiple experimental techniques at the Shanghai Synchrotron Radiation Facility.The undulators consisted of an elliptically polarizing undulator and in-vacuum undulator that generate the soft and hard X-rays, respectively. The beamline covered a wide energy range from 130 to 18 ke V with both a high photon flux([ 10^(12) phs/s with exit silt 30 lm in soft X-ray and [ 5 9 10^(12) phs/s in hard X-ray within 0.1%BW bandwidth) and promising resolving power(maximum E/DE [ 15,000 in soft X-ray with exit silt 30 lm and [6000 in hard X-ray). Moreover, the beam spots from the soft and hard X-rays were focused to the same sample position with a high overlap ratio, so that the surfaces, interfaces, and bulk properties were characterized in situ by changing the probing depth.
文摘目的探讨上皮性细胞粘附分子(E-cadherin,E-cad)在人卵巢浆液性囊腺癌高低转移细胞株HO-8910PM及HO-8910中的表达及生物学行为的差异。方法采用免疫荧光法及W estern b lotting检测细胞中E-cad蛋白表达的差异,用粘附实验检测细胞与细胞外基质的粘附能力,用Transwell小室法检测细胞侵袭能力及迁移能力。结果E-cad在HO-8910细胞中为高表达,在HO-8910PM细胞中为低表达,HO-8910PM细胞与细胞外基质的粘附能力、侵袭能力及迁移能力均明显高于HO-8910细胞(P<0.05,P<0.01)。结论人卵巢浆液性曩腺癌HO-8910PM细胞的浸润、转移等恶性行为可能与E-cad的表达下降有关。
