Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application p...Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application potential in information encryption,anti-counterfeiting,but remained challenging in improving the security.Herein,we described a self-erased time-resolved information encryption via using photoswitchable dual-color fluorescent polymeric nanoparticles(PDFPNs)containing two fluorescence dyes(blue and red)and photochromic spiroxazine derivatives.In view of the different thermo-induced isomerization rates of photochromic spiroxazine derivatives in different flexible substrates,the decoloration rate of PDFPNs can be programmatically tuned by regulating ratio between rigid polymer and flexible polymer.Therefore,after ultraviolet light(UV)irradiation,correct information could only be recognized in preestablished time during the self-erased process.Our results indicated that PDFPNs exhibited fast photo-responsibility(2 min),high fluorescence contrast,well-pleasing photo-reversibility(>20 times),and programmable thermo-responsiveness(24 s-6 h).We thus demonstrated their application in the selferased time-resolved information encryption and anti-counterfeiting with high security.展开更多
A long-/long-wave dual-color detector with N-M-π-B-π-M-N structure was developed based on a type-ⅡInAs/GaSb superlattice.The saturated responsivity was achieved under low bias voltage for both channels.The device c...A long-/long-wave dual-color detector with N-M-π-B-π-M-N structure was developed based on a type-ⅡInAs/GaSb superlattice.The saturated responsivity was achieved under low bias voltage for both channels.The device could be operated as a single detector for sequential detection and showed high quantum efficiencies.The peak quantum efficiencies of long-wavelength infrared band-1(blue channel)and long-wavelength infrared band-2(red channel)were 44%at 6.3μm under 20 mV and 57%at 9.1μm under-60 mV,respectively.The optical performance for each channel was achieved using a 2μm thickness absorber.Due to the high QE,the specific detectivities of the blue and red channels reached5.0×10^(11)cm·Hz^(1/2)/W at 6.8μm and 3.1×10^(11)cm·Hz1^(1/2)/W at 9.1μm,respectively,at 77 K.展开更多
An ultraviolet-infrared dual-color detector is proposed and realized based on the vertical integration of single-layer graphene and a 4 H-SiC layer by semiconductor micro-fabrication technology. The spectral response ...An ultraviolet-infrared dual-color detector is proposed and realized based on the vertical integration of single-layer graphene and a 4 H-SiC layer by semiconductor micro-fabrication technology. The spectral response characteristics of the detector are analyzed. The ultraviolet response range is 208—356 nm with a responsivity larger than 0.4 mA/W and the infrared response range is 1.016—1.17 μm with a responsivity larger than 0.4 mA/W at room temperature and 5 V bias voltage. The peak responsivity of the graphene in the ultraviolet-C band at 232 nm is 0.73 mA/W and in the near infrared band at 1.148 μm is 0.64 mA/W. The peak responsivity of SiC layer in the ultraviolet-B band at 312 nm is 2.27 mA/W. Besides, the responsivity increases with the bias voltage.展开更多
Dual-color(blue and green) InGaN/GaN nanorod light-emitting diodes(LEDs) with three different nanorod diameters are fabricated. Enhancement of luminescence intensity per area is observed in blue and green wells,to var...Dual-color(blue and green) InGaN/GaN nanorod light-emitting diodes(LEDs) with three different nanorod diameters are fabricated. Enhancement of luminescence intensity per area is observed in blue and green wells,to varying degrees. When the diameter is 40 nm, it sharply decreases, which could be explained by the sidewall nonradiative recombination. Time-resolved photoluminescence is conducted to study the carrier lifetime. High recombination rate is observed in nanorod arrays, and is an order of magnitude less than that of the planar LED.When the diameter is 40 nm, the nonradiative lifetime decreases, and this explains the decrease of intensity. The3 D-FDTD simulations show the enhancement of light extraction out of geometry structure by calculating the transmittance of the nanorod arrays.展开更多
Cascading reactions in fluorophores accompanied by the replacement of different fluorescence wavelengths can be used to develop luminescent materials and reactive fluorescent probes.Based on multiple signal channels,t...Cascading reactions in fluorophores accompanied by the replacement of different fluorescence wavelengths can be used to develop luminescent materials and reactive fluorescent probes.Based on multiple signal channels,the selectivity of probes can be improved and the range of response to vip molecule recognition can be expanded.By regulating the position,number,and activity of active sites in fluorophores,fluorescent probes that successively react with thiol and amino groups in cysteine(Cys),homocysteine(Hcy)have been developed,which can only react with the thiol group of GSH,In this paper,we report the first probe capable of cascading nucleophilic substitution reaction with the thiol group and amino group of GSH at a single reaction site,and showed the dual-color recognition of GSH,which improved the selectivity of GSH also was an extension of GSH probes.The probe Rho-DEA was based on a TICS fluorophore,and the intramolecular cascade nucleophilic substitution reaction occurs with Cys/Hcy.The thiol substitution of the first step reaction with Cys/Hcy was quenched due to intersystem crossing to triplet state,so GSH can be selectively recognized from the fluorescence signal.Rho-DEA has the ability of mitochondrial localization,and finally realized in situ dual-color fluorescence recognition of GSH in mitochondria.展开更多
Clathrin-and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors.However,due to their dimensions are within the diffraction limit,traditional fluorescen...Clathrin-and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors.However,due to their dimensions are within the diffraction limit,traditional fluorescence microscopy cannot distinguish them and little is known about their interactions underneath cell membrane.In this study,we proposed the line-switching scanning imaging mode for dual-color triplet-state relaxation(T-Rex)stimulated emission depletion(STED)super-resolution microscopy.With this line-switching mode,the cross-talk between the two channels,the side effects from pulse picker and image drift in frame scanning mode can be effectively eliminated.The dual-color super-resolution imaging results in mixed fluorescent beads validated the excellent performance.With this super-resolution microscope,not only the ring-shaped structure of clathrin and caveolae endocytic vesicles,but also their semi-fused structures underneath the cell membrane were distinguished clearly.The resultant infor-mation will greatly facilitate the study of clathrin-and caveolae-mediated receptor endocytosis and signaling process and also our home-built dual-color T-Rex STED microscope with this line-switching imaging mode provides a precise and convenient way to study subcellular-scale protein interactions.展开更多
The coherent control of field-free molecular orientation of CO with combined femtosecond single- and dual-color laser pulses has been theoretically studied. The effect of the delay time between the femtosecond single-...The coherent control of field-free molecular orientation of CO with combined femtosecond single- and dual-color laser pulses has been theoretically studied. The effect of the delay time between the femtosecond single- and dual-color laser pulses is discussed, and the physical mechanism of the enhancement of molecular orientation with pre-alignment of the molecule is investigated. It is found that the basic mechanism is based on the creation of a rotational wave packet by the femtosecond single-color laser pulse. Furthermore, we investigate the interference between multiple rotational excitation pathways following pre-alignment with femtosecond single-color laser pulse. It is shown that such interference can lead to an enhancement of the orientation of CO molecule by a factor of 1.6.展开更多
Mn-doped all-inorganic perovskite quantum dots (QDs) provide prominent applications in the fields of low-cost light source or display,because of their remarkable properties including dual-color emission and reduced le...Mn-doped all-inorganic perovskite quantum dots (QDs) provide prominent applications in the fields of low-cost light source or display,because of their remarkable properties including dual-color emission and reduced lead content,as well as high photoluminescence quantum yields (PLQYs) and high stability.However,the existing synthesis approaches usually require hash conditions,such as high temperature and nitrogen protection,which is a major hurdler for the practical manufacturing.In addition,the significantly high Mn substitution ratio in CsPbX3 QDs is still challenging.The real dual-color emission with two strong emission peaks in the Mn-doped all-inorganic perovskite QDs has attracted great interest.Here we present a gram-scale approach to synthesize both CsPbxMn1-xCI3 and CsPb1-xMnxClyBr3-y QDs at 100 ℃ in the air with high Mn substitution ratio,up to 55.64% atomically.The as-prepared CsPb1-xMnxClyBr3-y QDs exhibit high PLQYs of 62.41% and dual-color emission with two strong emission peaks around at 400-450 nm and 600 nm,respectively.The enhanced peak at 400-450 nm is a result of the hybrid halides in CsPbBrxCl3-x host.Furthermore,the unique advantage of the optical emission and high PLQYs properties of the CsPbxMn1-xCl3 QDs has been demonstrated as invisible ink for encryption applications and polymer composites.Our gram-scale synthesis approach for Mn-doped all-inorganic perovskite QDs may boost the future research and practical applications of QDs-based white LED,spintronics,and molecular barcoding.展开更多
A monolithic integrated ultraviolet-infrared(UV-IR) dual-color photodetector based on graphene/GaN heterojunction was fabricated by vertically integrating a GaN nanowire array on a silicon substrate with monolayer gra...A monolithic integrated ultraviolet-infrared(UV-IR) dual-color photodetector based on graphene/GaN heterojunction was fabricated by vertically integrating a GaN nanowire array on a silicon substrate with monolayer graphene. The device detects UV and IR lights by different mechanisms. The UV detection is accomplished by the forbidden band absorption of GaN, and the IR detection is realized by the free electron absorption of graphene. At peak wavelengths of 360 nm and 1540 nm, the detector has responsivities up to 6.93 A/W and 0.11 A/W, detection efficiencies of 1.23 × 1012 cm·Hz1/2·W-1 and 1.88 × 1010 cm·Hz1/2·W-1, respectively,and a short response time of less than 3 ms.展开更多
Herein,we presented a brand-new concept to construct the Forster resonance energy transfer(FRET)based cassette by integrating a vibration-induced emission(VIE)chromophore as the donor.Different from traditional donors...Herein,we presented a brand-new concept to construct the Forster resonance energy transfer(FRET)based cassette by integrating a vibration-induced emission(VIE)chromophore as the donor.Different from traditional donors only with a single emission,the VIE donor possessed well-separated dual emission bands by altering the excited state molecular configuration from the bent state to the planar state.By linking an acceptor such as a cyanine dye(Cy5),a novel VIE-FRET cassette(PPCy5)was prepared.The planar emission profile of the VIE donor moiety could fully cover the absorption of Cy5,and thus the complete FRET process enabled the excellent bimodal spectra difference of 142 nm and ultra-large pseudo-Stokes shift of up to 300 nm.Benefiting from the viscosity-dependent characteristic of the VIE donor,PPCy5 could clearly and intuitively reveal the different viscosity regions in vivo by dual-color and high-resolution imaging.The VIE-FRET paradigm provides an optional platform for developing donor-acceptor-based dual-color fluorescent probes with high-resolution imaging ability.展开更多
To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 19...To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 1992 to 2001 were diagnosed as MDS and acute myelocytic leukemia (AML) according to the French American British (FAB) criteria Chromosomes were prepared using the direct method as well as 24 hou r unstimulated cultures of fresh heparinized bone marrow for each subject, while R banding was used to analyze karyotypes Dual color fluorescence in situ hy bridization (FISH) using SpectrumRed and SpectrumGreen directly labeled chromoso me 1 specific α satellite DNA probe (red) and chromosome 7 specific α sat ellite DNA probe (green) was performed for three cases Results Of the five patients, three had 1;7 translocation due to a long history of expos ure to benzene In three cases, dual color FISH resulted in three red signals and two green ones, in which one red signal adjoining one green signal in 27 6% , 84% and 18 5% metaphases, respectively Conclusions Exposure to benzene may be the cause for Chinese MDS and AML patients with t(1;7 ) translocation The result of dual color FISH convincingly confirmed that the centromere of the derivative chromosome 7p/1q resulting from 1;7 translocation was made up of centromeres from both chromosomes 1 and 7展开更多
Physical unclonable functions(PUFs)offer a promising defensive measure against the escalating challenges posed by the increasingly rampant counterfeit products.Conventional PUF materials with a singular physical prope...Physical unclonable functions(PUFs)offer a promising defensive measure against the escalating challenges posed by the increasingly rampant counterfeit products.Conventional PUF materials with a singular physical property encounter limitations in encoding flexibility and capacity.Here,we propose a dual-color center diamond-based PUF(D-PUF)ink that exploits four diverse optical characteristics of dual-color center in diamond to design a concealable multi-level cryptographic authentication protocol.Through simple writing,stamping,or spraying,intricate covert random patterns can be directly generated on the objects,which are imperceptible under visible light.When challenged by a 532 nm laser,the D-PUF exhibits four distinct optical responses,including Raman,zero phonon line(ZPL)of germanium vacancies(GeV),ZPL of silicon vacancies(SiV),and the intensity ratios of these ZPLs.These responses were harvested simultaneously to construct the four-level separate encodable matrices.Furthermore,M-ary encoding algorithms were implemented to encrypt PUFs with flexibility.The resulting multi-level PUF system attains notable uniqueness,repeatability,extensive encoding capacity(>1048164/(100 pixels)2),and ultra-high information entropy(6 bits/pixel).This study inspires designing new generations of multi-level PUFs with enhanced coding flexibility and holds significant promise for applications in print security.展开更多
基金financially supported by the National Key R&D Program of China(Nos.2023YFB3812400,2023YFB3812403)National Natural Foundation of China(Nos.52273206,52350233)+1 种基金Hunan Provincial Natural Science Foundation(No.2021JJ10029)Huxiang High-level Talent Gathering Project(No.2022RC4039).
文摘Photoswitchable fluorescent polymeric nanoparticles were widely concerned because of their excellent features including the flexible design,easy preparation and functionalization,and thus exhibited great application potential in information encryption,anti-counterfeiting,but remained challenging in improving the security.Herein,we described a self-erased time-resolved information encryption via using photoswitchable dual-color fluorescent polymeric nanoparticles(PDFPNs)containing two fluorescence dyes(blue and red)and photochromic spiroxazine derivatives.In view of the different thermo-induced isomerization rates of photochromic spiroxazine derivatives in different flexible substrates,the decoloration rate of PDFPNs can be programmatically tuned by regulating ratio between rigid polymer and flexible polymer.Therefore,after ultraviolet light(UV)irradiation,correct information could only be recognized in preestablished time during the self-erased process.Our results indicated that PDFPNs exhibited fast photo-responsibility(2 min),high fluorescence contrast,well-pleasing photo-reversibility(>20 times),and programmable thermo-responsiveness(24 s-6 h).We thus demonstrated their application in the selferased time-resolved information encryption and anti-counterfeiting with high security.
基金supported by the National Key Technology R&D Program of China(Grant Nos.2018YFA0209104 and 2016YFB0402403)
文摘A long-/long-wave dual-color detector with N-M-π-B-π-M-N structure was developed based on a type-ⅡInAs/GaSb superlattice.The saturated responsivity was achieved under low bias voltage for both channels.The device could be operated as a single detector for sequential detection and showed high quantum efficiencies.The peak quantum efficiencies of long-wavelength infrared band-1(blue channel)and long-wavelength infrared band-2(red channel)were 44%at 6.3μm under 20 mV and 57%at 9.1μm under-60 mV,respectively.The optical performance for each channel was achieved using a 2μm thickness absorber.Due to the high QE,the specific detectivities of the blue and red channels reached5.0×10^(11)cm·Hz^(1/2)/W at 6.8μm and 3.1×10^(11)cm·Hz1^(1/2)/W at 9.1μm,respectively,at 77 K.
基金supported by the National Natural Science Foundation of China(No.61804166)
文摘An ultraviolet-infrared dual-color detector is proposed and realized based on the vertical integration of single-layer graphene and a 4 H-SiC layer by semiconductor micro-fabrication technology. The spectral response characteristics of the detector are analyzed. The ultraviolet response range is 208—356 nm with a responsivity larger than 0.4 mA/W and the infrared response range is 1.016—1.17 μm with a responsivity larger than 0.4 mA/W at room temperature and 5 V bias voltage. The peak responsivity of the graphene in the ultraviolet-C band at 232 nm is 0.73 mA/W and in the near infrared band at 1.148 μm is 0.64 mA/W. The peak responsivity of SiC layer in the ultraviolet-B band at 312 nm is 2.27 mA/W. Besides, the responsivity increases with the bias voltage.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61505197 and 61334009the National Science and Technology Major Project under Grant No 2017YFB0403803
文摘Dual-color(blue and green) InGaN/GaN nanorod light-emitting diodes(LEDs) with three different nanorod diameters are fabricated. Enhancement of luminescence intensity per area is observed in blue and green wells,to varying degrees. When the diameter is 40 nm, it sharply decreases, which could be explained by the sidewall nonradiative recombination. Time-resolved photoluminescence is conducted to study the carrier lifetime. High recombination rate is observed in nanorod arrays, and is an order of magnitude less than that of the planar LED.When the diameter is 40 nm, the nonradiative lifetime decreases, and this explains the decrease of intensity. The3 D-FDTD simulations show the enhancement of light extraction out of geometry structure by calculating the transmittance of the nanorod arrays.
基金supported by the National Natural Science Foundation of China(Nos.22078314,21878286,21908216)。
文摘Cascading reactions in fluorophores accompanied by the replacement of different fluorescence wavelengths can be used to develop luminescent materials and reactive fluorescent probes.Based on multiple signal channels,the selectivity of probes can be improved and the range of response to vip molecule recognition can be expanded.By regulating the position,number,and activity of active sites in fluorophores,fluorescent probes that successively react with thiol and amino groups in cysteine(Cys),homocysteine(Hcy)have been developed,which can only react with the thiol group of GSH,In this paper,we report the first probe capable of cascading nucleophilic substitution reaction with the thiol group and amino group of GSH at a single reaction site,and showed the dual-color recognition of GSH,which improved the selectivity of GSH also was an extension of GSH probes.The probe Rho-DEA was based on a TICS fluorophore,and the intramolecular cascade nucleophilic substitution reaction occurs with Cys/Hcy.The thiol substitution of the first step reaction with Cys/Hcy was quenched due to intersystem crossing to triplet state,so GSH can be selectively recognized from the fluorescence signal.Rho-DEA has the ability of mitochondrial localization,and finally realized in situ dual-color fluorescence recognition of GSH in mitochondria.
基金This work was supported by the CAS Key Technology Talent Programthe Instrument Incubation Program of Institute of Chemistry,CAS+3 种基金the National Natural Science Foundation of China(21735006,32000873,22077124 and 91939301)Beijing Natural Science Foundation(5184032)the China Postdoctoral Science Foundation(2019M650718)the Chinese Academy of Sciences.
文摘Clathrin-and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors.However,due to their dimensions are within the diffraction limit,traditional fluorescence microscopy cannot distinguish them and little is known about their interactions underneath cell membrane.In this study,we proposed the line-switching scanning imaging mode for dual-color triplet-state relaxation(T-Rex)stimulated emission depletion(STED)super-resolution microscopy.With this line-switching mode,the cross-talk between the two channels,the side effects from pulse picker and image drift in frame scanning mode can be effectively eliminated.The dual-color super-resolution imaging results in mixed fluorescent beads validated the excellent performance.With this super-resolution microscope,not only the ring-shaped structure of clathrin and caveolae endocytic vesicles,but also their semi-fused structures underneath the cell membrane were distinguished clearly.The resultant infor-mation will greatly facilitate the study of clathrin-and caveolae-mediated receptor endocytosis and signaling process and also our home-built dual-color T-Rex STED microscope with this line-switching imaging mode provides a precise and convenient way to study subcellular-scale protein interactions.
基金Project supported by the National Natural Science Foundation of China （Grant Nos. 61077037 and 11274096）, the Doctoral Research Fund of Henan Normal University, China （Grant No. qd 12109）, and the Key Scientific and Basic Technology Research Program of Henan Province, China （Grant Nos. 102102210184 and 122300410109）.
文摘The coherent control of field-free molecular orientation of CO with combined femtosecond single- and dual-color laser pulses has been theoretically studied. The effect of the delay time between the femtosecond single- and dual-color laser pulses is discussed, and the physical mechanism of the enhancement of molecular orientation with pre-alignment of the molecule is investigated. It is found that the basic mechanism is based on the creation of a rotational wave packet by the femtosecond single-color laser pulse. Furthermore, we investigate the interference between multiple rotational excitation pathways following pre-alignment with femtosecond single-color laser pulse. It is shown that such interference can lead to an enhancement of the orientation of CO molecule by a factor of 1.6.
基金supported by the National Key Research and Development Program of China (No.2018YFB1105100)the National Natural Science Foundation of China (Nos.51572096 and 61704061).
文摘Mn-doped all-inorganic perovskite quantum dots (QDs) provide prominent applications in the fields of low-cost light source or display,because of their remarkable properties including dual-color emission and reduced lead content,as well as high photoluminescence quantum yields (PLQYs) and high stability.However,the existing synthesis approaches usually require hash conditions,such as high temperature and nitrogen protection,which is a major hurdler for the practical manufacturing.In addition,the significantly high Mn substitution ratio in CsPbX3 QDs is still challenging.The real dual-color emission with two strong emission peaks in the Mn-doped all-inorganic perovskite QDs has attracted great interest.Here we present a gram-scale approach to synthesize both CsPbxMn1-xCI3 and CsPb1-xMnxClyBr3-y QDs at 100 ℃ in the air with high Mn substitution ratio,up to 55.64% atomically.The as-prepared CsPb1-xMnxClyBr3-y QDs exhibit high PLQYs of 62.41% and dual-color emission with two strong emission peaks around at 400-450 nm and 600 nm,respectively.The enhanced peak at 400-450 nm is a result of the hybrid halides in CsPbBrxCl3-x host.Furthermore,the unique advantage of the optical emission and high PLQYs properties of the CsPbxMn1-xCl3 QDs has been demonstrated as invisible ink for encryption applications and polymer composites.Our gram-scale synthesis approach for Mn-doped all-inorganic perovskite QDs may boost the future research and practical applications of QDs-based white LED,spintronics,and molecular barcoding.
基金supported by the National Natural Science Foundation of China (Nos. 61574161 and 61574130)the Natural Science Foundation of Jiangsu Province,China (No. BK20180252)the Department of Science and Technology of Jilin Province,China(No. 20180520177JH)。
文摘A monolithic integrated ultraviolet-infrared(UV-IR) dual-color photodetector based on graphene/GaN heterojunction was fabricated by vertically integrating a GaN nanowire array on a silicon substrate with monolayer graphene. The device detects UV and IR lights by different mechanisms. The UV detection is accomplished by the forbidden band absorption of GaN, and the IR detection is realized by the free electron absorption of graphene. At peak wavelengths of 360 nm and 1540 nm, the detector has responsivities up to 6.93 A/W and 0.11 A/W, detection efficiencies of 1.23 × 1012 cm·Hz1/2·W-1 and 1.88 × 1010 cm·Hz1/2·W-1, respectively,and a short response time of less than 3 ms.
基金This work was supported by the National Natural Science Foundation of China(21788102,21905090,21790361,22004036,91859205)Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)+2 种基金the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning,Programme of Introducing Talents of Discipline to Universities(B16017)Shanghai Science and Technology Committee(17520750100)Natural Science Foundation of Shanghai(19ZR1412200),and the Fundamental Research Funds for the Central Universities.
文摘Herein,we presented a brand-new concept to construct the Forster resonance energy transfer(FRET)based cassette by integrating a vibration-induced emission(VIE)chromophore as the donor.Different from traditional donors only with a single emission,the VIE donor possessed well-separated dual emission bands by altering the excited state molecular configuration from the bent state to the planar state.By linking an acceptor such as a cyanine dye(Cy5),a novel VIE-FRET cassette(PPCy5)was prepared.The planar emission profile of the VIE donor moiety could fully cover the absorption of Cy5,and thus the complete FRET process enabled the excellent bimodal spectra difference of 142 nm and ultra-large pseudo-Stokes shift of up to 300 nm.Benefiting from the viscosity-dependent characteristic of the VIE donor,PPCy5 could clearly and intuitively reveal the different viscosity regions in vivo by dual-color and high-resolution imaging.The VIE-FRET paradigm provides an optional platform for developing donor-acceptor-based dual-color fluorescent probes with high-resolution imaging ability.
文摘To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 1992 to 2001 were diagnosed as MDS and acute myelocytic leukemia (AML) according to the French American British (FAB) criteria Chromosomes were prepared using the direct method as well as 24 hou r unstimulated cultures of fresh heparinized bone marrow for each subject, while R banding was used to analyze karyotypes Dual color fluorescence in situ hy bridization (FISH) using SpectrumRed and SpectrumGreen directly labeled chromoso me 1 specific α satellite DNA probe (red) and chromosome 7 specific α sat ellite DNA probe (green) was performed for three cases Results Of the five patients, three had 1;7 translocation due to a long history of expos ure to benzene In three cases, dual color FISH resulted in three red signals and two green ones, in which one red signal adjoining one green signal in 27 6% , 84% and 18 5% metaphases, respectively Conclusions Exposure to benzene may be the cause for Chinese MDS and AML patients with t(1;7 ) translocation The result of dual color FISH convincingly confirmed that the centromere of the derivative chromosome 7p/1q resulting from 1;7 translocation was made up of centromeres from both chromosomes 1 and 7
基金the support of the National Key Research and Development Program of China(No.2022YFB3608604)the National Natural Science Foundation of China(Nos.U22A2077 and U21A2070)+5 种基金Shandong Provincial Natural Science Foundation(No.ZR2022MH280)Medical Science and Technology Project of Shandong Province(No.202307020692)Natural Science Foundation of Henan(Nos.212300410020 and 222300420297)Science and Technology Major Project of Henan Province(No.221100230300)the Fundamental Research Fund of Henan Academy of Sciences(No.20250627005)Henan Postdoctoral Foundation(No.202101007).
文摘Physical unclonable functions(PUFs)offer a promising defensive measure against the escalating challenges posed by the increasingly rampant counterfeit products.Conventional PUF materials with a singular physical property encounter limitations in encoding flexibility and capacity.Here,we propose a dual-color center diamond-based PUF(D-PUF)ink that exploits four diverse optical characteristics of dual-color center in diamond to design a concealable multi-level cryptographic authentication protocol.Through simple writing,stamping,or spraying,intricate covert random patterns can be directly generated on the objects,which are imperceptible under visible light.When challenged by a 532 nm laser,the D-PUF exhibits four distinct optical responses,including Raman,zero phonon line(ZPL)of germanium vacancies(GeV),ZPL of silicon vacancies(SiV),and the intensity ratios of these ZPLs.These responses were harvested simultaneously to construct the four-level separate encodable matrices.Furthermore,M-ary encoding algorithms were implemented to encrypt PUFs with flexibility.The resulting multi-level PUF system attains notable uniqueness,repeatability,extensive encoding capacity(>1048164/(100 pixels)2),and ultra-high information entropy(6 bits/pixel).This study inspires designing new generations of multi-level PUFs with enhanced coding flexibility and holds significant promise for applications in print security.
