Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by ma...Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by manual pipetting or liquid handling that compromises organoid-organoid homogeneity and organoid-tissue consistency.Droplet-based microfluidics enables automated organoid production with high organoid-organoid homogeneity,organoid-tissue consistency,and a significantly improved production spectrum.It takes advantage of droplet-encapsulation of defined populations of cells and droplet-rendered microstructures that guide cell self-organization.Herein,we studied the droplet-engineered organoids(DEOs),derived from mouse liver tissues and human liver tumors,by using transcriptional analysis and cellular deconvolution on bulk RNA-seq data.The characteristics of DEOs are compared with the parental liver tissues(or tumors)and NEOs.The DEOs are proven higher reproducibility and consistency with the parental tissues,have a high production spectrum and shortened modeling time,and possess inter-organoid homogeneity and inter-tumor cell heterogeneity.展开更多
基金supported by the National Natural Science Foundation of China(61971255 and 82111530212)the Natural Science Foundation of Guangdong Province(2021B1515020092)+1 种基金the Shenzhen Science and Technology Innovation Commission(RCYX20200714114736146,WDZC20200821141349001,KCXFZ20201221173207022,KCXFZ20200201101050887)the Shenzhen Bay Laboratory Fund(SZBL2020090501014).
文摘Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by manual pipetting or liquid handling that compromises organoid-organoid homogeneity and organoid-tissue consistency.Droplet-based microfluidics enables automated organoid production with high organoid-organoid homogeneity,organoid-tissue consistency,and a significantly improved production spectrum.It takes advantage of droplet-encapsulation of defined populations of cells and droplet-rendered microstructures that guide cell self-organization.Herein,we studied the droplet-engineered organoids(DEOs),derived from mouse liver tissues and human liver tumors,by using transcriptional analysis and cellular deconvolution on bulk RNA-seq data.The characteristics of DEOs are compared with the parental liver tissues(or tumors)and NEOs.The DEOs are proven higher reproducibility and consistency with the parental tissues,have a high production spectrum and shortened modeling time,and possess inter-organoid homogeneity and inter-tumor cell heterogeneity.
