Droplet-based microfluidic technology can be utilized as a microreactor to prepare novel functional monodisperse microcapsules.In this study,a droplet-based microfluidic chip with surface modification,which allowed th...Droplet-based microfluidic technology can be utilized as a microreactor to prepare novel functional monodisperse microcapsules.In this study,a droplet-based microfluidic chip with surface modification,which allowed the one-step preparation of double emulsion microcapsules.An O/W/O double emulsion using polyethylene(glycol)diacrylate(PEGDA)solution as the intermediate water phase was prepared by regulating the hydrophilicity and hydrophobicity of the chip surface,with PEGDA microcapsules prepared using UV polymerization.And then anti-tumor drug paclitaxel and neurotoxin 6-OHDA were encapsulated in microcapsules for drug and toxicology evaluation,respectively.Compared to controls,drug-loaded mi-crocapsules caused a significant increase in the death rate of PC12 cells.This indicates that the obtained drug-loaded microcapsules could be used in drug evaluation and potentially in drug screening and deliv-ery.展开更多
The transistor-inspired Droplet-based Electricity Generator(DEG)significantly enhances the energy collection efficiency from single-position droplets.However,the design of the DEG arrays combining high output performa...The transistor-inspired Droplet-based Electricity Generator(DEG)significantly enhances the energy collection efficiency from single-position droplets.However,the design of the DEG arrays combining high output performance and large-scale integration under multi-position droplet impacts remains a challenge.Inspired by the unique structure of the honeycomb,we developed an Independent-Cell Droplet-based Electricity Generator(IC-DEG)array that allows for high-efficiency and stable droplet energy harvesting under multi-position droplet impacts.Each independent cell is a transistor-inspired Tubular Droplet-based Electricity Generator(T-DEG),which ensures the high electrical output of the IC-DEG array.The honeycomb-like arrangement improves the space utilization,accelerates the detachment of droplets,and avoids electrical interference among independent cells,all of which further enhance the IC-DEG array performance.The average peak open-circuit voltage of the IC-DEG array is 265.2 V,and 96.6%of peak voltages exceed 200 V,almost double that of a traditional planar array.Moreover,the average droplet detachment time of the IC-DEG array is 44.8 ms,41.4%shorter than the traditional planar array.The enhanced performance of the IC-DEG array is further demonstrated by the high speed of charging capacitors and the capability of driving electronic devices.This study provides a promising design concept for large-scale droplet energy harvesting devices.展开更多
Microproteomics, the profiling of protein expressions in small cell populations or individual cells, is essential for understanding complex biological systems. However, sample loss and insufficient sensitivity of anal...Microproteomics, the profiling of protein expressions in small cell populations or individual cells, is essential for understanding complex biological systems. However, sample loss and insufficient sensitivity of analytical techniques pose severe challenges to this field. Microfluidics, particularly droplet-based microfluidics, provides an ideal approach by enabling miniaturized and integrated workflows to process samples and offers several advantages, including reduced sample loss, low reagent consumption, faster reaction times, and improved throughput. Droplet-based microfluidics manipulates droplets of fluids to function as discrete reaction units, enabling complex chemical reactions and biological workflows in a miniaturized setting. This article discusses a variety of on-chip functions of droplet-based microfluidics,including cell sorting, cell culture, and sample processing. We then highlight recent advances in the mass spectrometry(MS)-based analysis of single cells using droplet-based microfluidic platforms, including digital microfluidics(DMF). Finally, we review the integrated DMF–MS systems that enable automated and parallel proteomic profiling of single cells with high sensitivity and discuss the applications of the technology and its future perspectives.展开更多
Lead halide perovskite nanocrystals(NCs)exhibit high photoluminescence quantum yield(PLQY),high defect tolerance,narrow half peak width,and wide luminous gamut,making them the ideal optoelectronic materials in numerou...Lead halide perovskite nanocrystals(NCs)exhibit high photoluminescence quantum yield(PLQY),high defect tolerance,narrow half peak width,and wide luminous gamut,making them the ideal optoelectronic materials in numerous fields.Nonetheless,their production still suffers from the limited productivity at the bench level.In this work,we fabricated CsPbX3(X=Cl,Br,I)NCs within droplet-based micro-reactors,where both the nucleation and growth processes could be precisely controlled inside 130-nL microdroplets.This provides a new paradigm for the large-scale synthesis of perovskite NCs with high PLQY.Compared with other synthetic methods,this method can increase the concentration of reactant precursors by 3±116 times,while lowering the ligand to reactant ratio to 2%±50%of the commonly used hot-injection method.By modulating the reaction temperature and residence time,the structure-function relationship between the morphology of NCs and PL properties was extensively investigated.The microfluidic-based process allows the flexible adjustment in the proportion of PbX2 precursors to achieve the fabrication of perovskite NCs whose luminescence range covers the entire visible spectrum(406±677 nm)within one reaction.Finally,perovskite NCs with different halide ions were encapsulated in polymethyl methacrylate to prepare a colored light-emitting diode strip.展开更多
Droplet-based electricity generators (DEGs) leveraging triboelectric effects are simple and high-performance devices for harvesting energy from ubiquitous water droplets. Instantaneous power plays a vital role in wide...Droplet-based electricity generators (DEGs) leveraging triboelectric effects are simple and high-performance devices for harvesting energy from ubiquitous water droplets. Instantaneous power plays a vital role in wide applications of DEGs. However, the governing law of the maximum instantaneous power and matching resistance is lacking and their determination suffers from heavy repetitive experiments, hindering the development of DEGs. Herein, we propose a quick evaluation method for the internal droplet impedance, instantaneous peak power, maximum instantaneous power and matching resistance which exhibits broad universality and excellent accuracy. Moreover, effects of diverse factors pertaining to droplets and devices are fully investigated, highlighting that the maximum instantaneous power and matching resistance can be effectively regulated across multiple orders of magnitudes by controlling the salt concentration. Our findings shed insights into the understanding, evaluation, and regulation of instantaneous power for DEGs, and shall promote the renovation of the DEG technology.展开更多
The contact electrification(CE)between DI water and SiO_(2)or fluorinated polymer has been proven to be mainly due to electron transfer,which is significantly influenced by ions in solution.However,how these ions in w...The contact electrification(CE)between DI water and SiO_(2)or fluorinated polymer has been proven to be mainly due to electron transfer,which is significantly influenced by ions in solution.However,how these ions in water affect the charge transfer at the liquid-solid(L-S)interface is still unresolved,especially for the already charged friction layer.Here,a direct current droplet-based electricity generator(DC-DEG)which is sensitive to the change of charge transfer at the L-S interface is adopted to detect the effects of ions in the neutral salt solution on the charged PTFE surface.The distribution of ions on the charged L-S interface(the change of electric potential on the solid surface)and its effects on the output of DC-DEGs have been studied.The results indicate that the charge transfer of droplets and then the output of DC-DEGs are closely related to the concentrations of salt solutions.Anions can enhance the surface potential of PTFE due to their adsorptions on PFTE while cations can reduce it due to their screen effect.At low ionic concentrations,the surface potential enhancement caused by anion adsorption is larger than that surface potential reduction caused by screen effect from cations.At high ionic concentrations,the electrostatic screen effect of cations increases a lot to weaken the surface potential and reducing the charge separation of droplets induced by electrostatic induction(EI).This work explains the redistribution process of ions at the L-S interface and also provides a clever solution for improving the electrical output performance of DEGs.展开更多
Biomanufacturing of tissues/organs in vitro is our big dream,driven by two needs:organ transplantation and accurate tissue models.Over the last decades,3D bioprinting has been widely applied in the construction of man...Biomanufacturing of tissues/organs in vitro is our big dream,driven by two needs:organ transplantation and accurate tissue models.Over the last decades,3D bioprinting has been widely applied in the construction of many tissues/organs such as skins,vessels,hearts,etc.,which can not only lay a foundation for the grand goal of organ replacement,but also be served as in vitro models committed to pharmacokinetics,drug screening and so on.As organs are so complicated,many bioprinting methods are exploited to figure out the challenges of different applications.So the question is how to choose the suitable bioprinting method?Herein,we systematically review the evolution,process and classification of 3D bioprinting with an emphasis on the fundamental printing principles and commercialized bioprinters.We summarize and classify extrusion-based,dropletbased,and photocuring-based bioprinting methods and give some advices for applications.Among them,coaxial and multi-material bioprinting are highlighted and basic principles of designing bioinks are also discussed.展开更多
The mechanism of microdroplet coalescence is a fundamental issue for droplet-based microfluidics. We developed an asymmetric expansion (a rectangular groove) along one side of a microchannel to achieve multiple-microd...The mechanism of microdroplet coalescence is a fundamental issue for droplet-based microfluidics. We developed an asymmetric expansion (a rectangular groove) along one side of a microchannel to achieve multiple-microdroplet trapping, collision, and coalescence. Compared with reported symmetric expansions, this asymmetric groove could easily trap microdroplets and control two or three microdroplet coalescences precisely without a requirement for temporal and spatial synchronization. To reveal the mechanisms of multiple-droplet coalescences in a groove, we observed five different coalescence patterns under different flow conditions. Moreover, we characterized the flow behavior quantitatively by simulating the velocity vector fields in both the microdroplets and continuous phase, finding good agreement with experiments. Finally, a map of coalescence forms with different capillary numbers () and flow ratios () was obtained. The results could provide a useful guidance for the design and application of droplet-based microfluidic devices.展开更多
Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted reproduction.Recent times have witnessed a...Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted reproduction.Recent times have witnessed an increase in the clinical demand of these applications,requiring urgent improvements in cell cryopreservation.However,cryopreservation technology suff ers from the issues of low cryopreservation effi ciency and cryoprotectant(CPA)toxicity.Application of advanced biotechnology tools can signifi cantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs,thus promoting the development of cryopreservation.Herein,based on the diff erent cryopreservation mechanisms available,we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation,including trehalose delivery,hydrogel-based cell encapsulation technique,droplet-based cell printing,and nanowarming,and also discuss the associated challenges and perspectives for future development.展开更多
The compartimentation of fluids in the microliter, nanoliter and picoliter range leads recently to many applications of microfluidics in material development, diagnostics and biological screenings. Droplet-based micro...The compartimentation of fluids in the microliter, nanoliter and picoliter range leads recently to many applications of microfluidics in material development, diagnostics and biological screenings. Droplet-based microfluidics allows the improvement of nanoparticle homogeneity and the tuning of particle properties. It supports combinatorial synthesis of inorganic as well as organic substances and can be applied for the cultivation and screening of bacteria, eucaryotic cells and fish embryos. The well-ordered handling and the addressing of microfluid segments improves the information transfer between chemical, biological and electronic systems. Despite this remarkable technical progress, there is a particular importance of microfluidics for future nanotechnological solutions. The hierarchical spatial organization of liquids, particles and gels in microfluidics represents a fundamental biomimetic principle which overcomes the limits of planar technology and opens the gate for realizing complex structured threedimensional nanoarchitectures. Recent applications of microstructured fluids in chemistry and biology and concepts for future developments will be discussed.展开更多
Sustainable power sources for outdoor wearable electronics are essential for the continuous operation of wearable devices.However,the current lack of engineering design that can harvest energy regardless of weather co...Sustainable power sources for outdoor wearable electronics are essential for the continuous operation of wearable devices.However,the current lack of engineering design that can harvest energy regardless of weather conditions presents a significant challenge.In this regard,this study introduces a wearable,breathable all-weather usable dual energy harvester(AWuDEH)that can seamlessly generate electrical energy regardless of weather conditions.In this study,the AWuDEH integrated with the thermoelectric generator and the droplet-based electricity generator is demonstrated.The AWuDEH,especially engineered with a bi-functional top substrate for radiative cooling and electrification,achieves sustainable energy harvesting outdoors,thereby addressing the conventional challenge associated with the necessity for separate energy harvesters tailored to outdoor usage contingent on weather conditions.The device reaches a maximum power output of 14.6μW cm^(-2)under simulated sunny conditions and generates a much more enhanced thermoelectric power of 74.78μW cm^(-2)and a droplet-based electric power of 256.25 mW m^(-2)in rainy conditions.As proof,this study developed self-powered wearable electronics capable of acquiring physiological signals in simulated outdoor scenarios.This study presents a promising advancement in wearable technology,offering a potent solution for sustainable energy harvesting independent of weather conditions.展开更多
Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by ma...Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by manual pipetting or liquid handling that compromises organoid-organoid homogeneity and organoid-tissue consistency.Droplet-based microfluidics enables automated organoid production with high organoid-organoid homogeneity,organoid-tissue consistency,and a significantly improved production spectrum.It takes advantage of droplet-encapsulation of defined populations of cells and droplet-rendered microstructures that guide cell self-organization.Herein,we studied the droplet-engineered organoids(DEOs),derived from mouse liver tissues and human liver tumors,by using transcriptional analysis and cellular deconvolution on bulk RNA-seq data.The characteristics of DEOs are compared with the parental liver tissues(or tumors)and NEOs.The DEOs are proven higher reproducibility and consistency with the parental tissues,have a high production spectrum and shortened modeling time,and possess inter-organoid homogeneity and inter-tumor cell heterogeneity.展开更多
基金supported by the National Natural Science Foun-dation of China(Nos.31800848 and 21775101).
文摘Droplet-based microfluidic technology can be utilized as a microreactor to prepare novel functional monodisperse microcapsules.In this study,a droplet-based microfluidic chip with surface modification,which allowed the one-step preparation of double emulsion microcapsules.An O/W/O double emulsion using polyethylene(glycol)diacrylate(PEGDA)solution as the intermediate water phase was prepared by regulating the hydrophilicity and hydrophobicity of the chip surface,with PEGDA microcapsules prepared using UV polymerization.And then anti-tumor drug paclitaxel and neurotoxin 6-OHDA were encapsulated in microcapsules for drug and toxicology evaluation,respectively.Compared to controls,drug-loaded mi-crocapsules caused a significant increase in the death rate of PC12 cells.This indicates that the obtained drug-loaded microcapsules could be used in drug evaluation and potentially in drug screening and deliv-ery.
基金supported by the National Key R&D Program of China(2022YFB4602401)the National Natural Science Foundation of China(52075071).
文摘The transistor-inspired Droplet-based Electricity Generator(DEG)significantly enhances the energy collection efficiency from single-position droplets.However,the design of the DEG arrays combining high output performance and large-scale integration under multi-position droplet impacts remains a challenge.Inspired by the unique structure of the honeycomb,we developed an Independent-Cell Droplet-based Electricity Generator(IC-DEG)array that allows for high-efficiency and stable droplet energy harvesting under multi-position droplet impacts.Each independent cell is a transistor-inspired Tubular Droplet-based Electricity Generator(T-DEG),which ensures the high electrical output of the IC-DEG array.The honeycomb-like arrangement improves the space utilization,accelerates the detachment of droplets,and avoids electrical interference among independent cells,all of which further enhance the IC-DEG array performance.The average peak open-circuit voltage of the IC-DEG array is 265.2 V,and 96.6%of peak voltages exceed 200 V,almost double that of a traditional planar array.Moreover,the average droplet detachment time of the IC-DEG array is 44.8 ms,41.4%shorter than the traditional planar array.The enhanced performance of the IC-DEG array is further demonstrated by the high speed of charging capacitors and the capability of driving electronic devices.This study provides a promising design concept for large-scale droplet energy harvesting devices.
基金supported by National Natural Science Foundation of China (62103050)National Key Research and Development Program of China (2022YFA1207100 and 2023YFE0112400)+3 种基金Beijing Natural Science Foundation (2242018)BIT Research and Innovation Promoting Project (2023CX01002)Open Research Fund of State Key Laboratory of Digital Medical Engineering (2023-K02)Open Research Fund of State Key Laboratory of Optoelectronic Materials and Technologies (Sun Yat-sen University) (OEMT-2022KF-09)。
文摘Microproteomics, the profiling of protein expressions in small cell populations or individual cells, is essential for understanding complex biological systems. However, sample loss and insufficient sensitivity of analytical techniques pose severe challenges to this field. Microfluidics, particularly droplet-based microfluidics, provides an ideal approach by enabling miniaturized and integrated workflows to process samples and offers several advantages, including reduced sample loss, low reagent consumption, faster reaction times, and improved throughput. Droplet-based microfluidics manipulates droplets of fluids to function as discrete reaction units, enabling complex chemical reactions and biological workflows in a miniaturized setting. This article discusses a variety of on-chip functions of droplet-based microfluidics,including cell sorting, cell culture, and sample processing. We then highlight recent advances in the mass spectrometry(MS)-based analysis of single cells using droplet-based microfluidic platforms, including digital microfluidics(DMF). Finally, we review the integrated DMF–MS systems that enable automated and parallel proteomic profiling of single cells with high sensitivity and discuss the applications of the technology and its future perspectives.
基金financially supported by the National Natural Science Foundation of China (22025801, 21991101, and 21736006)。
文摘Lead halide perovskite nanocrystals(NCs)exhibit high photoluminescence quantum yield(PLQY),high defect tolerance,narrow half peak width,and wide luminous gamut,making them the ideal optoelectronic materials in numerous fields.Nonetheless,their production still suffers from the limited productivity at the bench level.In this work,we fabricated CsPbX3(X=Cl,Br,I)NCs within droplet-based micro-reactors,where both the nucleation and growth processes could be precisely controlled inside 130-nL microdroplets.This provides a new paradigm for the large-scale synthesis of perovskite NCs with high PLQY.Compared with other synthetic methods,this method can increase the concentration of reactant precursors by 3±116 times,while lowering the ligand to reactant ratio to 2%±50%of the commonly used hot-injection method.By modulating the reaction temperature and residence time,the structure-function relationship between the morphology of NCs and PL properties was extensively investigated.The microfluidic-based process allows the flexible adjustment in the proportion of PbX2 precursors to achieve the fabrication of perovskite NCs whose luminescence range covers the entire visible spectrum(406±677 nm)within one reaction.Finally,perovskite NCs with different halide ions were encapsulated in polymethyl methacrylate to prepare a colored light-emitting diode strip.
基金financial support from the China Postdoctoral Science Foundation(No.2023TQ0210)the Postdoctoral Fellowship Program of CPSF(No.GZB20230403)+1 种基金the Fundamental Research Funds for the Central Universities(Shanghai Jiao Tong University)the Innovative Research Groups of the National Natural Science Foundation of China(No.51521004)。
文摘Droplet-based electricity generators (DEGs) leveraging triboelectric effects are simple and high-performance devices for harvesting energy from ubiquitous water droplets. Instantaneous power plays a vital role in wide applications of DEGs. However, the governing law of the maximum instantaneous power and matching resistance is lacking and their determination suffers from heavy repetitive experiments, hindering the development of DEGs. Herein, we propose a quick evaluation method for the internal droplet impedance, instantaneous peak power, maximum instantaneous power and matching resistance which exhibits broad universality and excellent accuracy. Moreover, effects of diverse factors pertaining to droplets and devices are fully investigated, highlighting that the maximum instantaneous power and matching resistance can be effectively regulated across multiple orders of magnitudes by controlling the salt concentration. Our findings shed insights into the understanding, evaluation, and regulation of instantaneous power for DEGs, and shall promote the renovation of the DEG technology.
基金supported by the National Natural Science Foundation of China(Grant No.12074321).
文摘The contact electrification(CE)between DI water and SiO_(2)or fluorinated polymer has been proven to be mainly due to electron transfer,which is significantly influenced by ions in solution.However,how these ions in water affect the charge transfer at the liquid-solid(L-S)interface is still unresolved,especially for the already charged friction layer.Here,a direct current droplet-based electricity generator(DC-DEG)which is sensitive to the change of charge transfer at the L-S interface is adopted to detect the effects of ions in the neutral salt solution on the charged PTFE surface.The distribution of ions on the charged L-S interface(the change of electric potential on the solid surface)and its effects on the output of DC-DEGs have been studied.The results indicate that the charge transfer of droplets and then the output of DC-DEGs are closely related to the concentrations of salt solutions.Anions can enhance the surface potential of PTFE due to their adsorptions on PFTE while cations can reduce it due to their screen effect.At low ionic concentrations,the surface potential enhancement caused by anion adsorption is larger than that surface potential reduction caused by screen effect from cations.At high ionic concentrations,the electrostatic screen effect of cations increases a lot to weaken the surface potential and reducing the charge separation of droplets induced by electrostatic induction(EI).This work explains the redistribution process of ions at the L-S interface and also provides a clever solution for improving the electrical output performance of DEGs.
基金sponsored by the National Natural Science Foundation of China(No.U1609207)。
文摘Biomanufacturing of tissues/organs in vitro is our big dream,driven by two needs:organ transplantation and accurate tissue models.Over the last decades,3D bioprinting has been widely applied in the construction of many tissues/organs such as skins,vessels,hearts,etc.,which can not only lay a foundation for the grand goal of organ replacement,but also be served as in vitro models committed to pharmacokinetics,drug screening and so on.As organs are so complicated,many bioprinting methods are exploited to figure out the challenges of different applications.So the question is how to choose the suitable bioprinting method?Herein,we systematically review the evolution,process and classification of 3D bioprinting with an emphasis on the fundamental printing principles and commercialized bioprinters.We summarize and classify extrusion-based,dropletbased,and photocuring-based bioprinting methods and give some advices for applications.Among them,coaxial and multi-material bioprinting are highlighted and basic principles of designing bioinks are also discussed.
基金supported by the Beijing Municipal Natural Science Foundation (Grant 7152012)the General Program of Science and Technology Development Project of the Beijing Municipal Education Commission (Grant KM201610005002)+2 种基金the National Natural Science Foundation of China (Grant 11572013)the China Scholarship Council (Grant 201406545031)the Training Plan of New Talent of Beijing University of Technology (Grant 2015-RX-L02)
文摘The mechanism of microdroplet coalescence is a fundamental issue for droplet-based microfluidics. We developed an asymmetric expansion (a rectangular groove) along one side of a microchannel to achieve multiple-microdroplet trapping, collision, and coalescence. Compared with reported symmetric expansions, this asymmetric groove could easily trap microdroplets and control two or three microdroplet coalescences precisely without a requirement for temporal and spatial synchronization. To reveal the mechanisms of multiple-droplet coalescences in a groove, we observed five different coalescence patterns under different flow conditions. Moreover, we characterized the flow behavior quantitatively by simulating the velocity vector fields in both the microdroplets and continuous phase, finding good agreement with experiments. Finally, a map of coalescence forms with different capillary numbers () and flow ratios () was obtained. The results could provide a useful guidance for the design and application of droplet-based microfluidic devices.
文摘Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted reproduction.Recent times have witnessed an increase in the clinical demand of these applications,requiring urgent improvements in cell cryopreservation.However,cryopreservation technology suff ers from the issues of low cryopreservation effi ciency and cryoprotectant(CPA)toxicity.Application of advanced biotechnology tools can signifi cantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs,thus promoting the development of cryopreservation.Herein,based on the diff erent cryopreservation mechanisms available,we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation,including trehalose delivery,hydrogel-based cell encapsulation technique,droplet-based cell printing,and nanowarming,and also discuss the associated challenges and perspectives for future development.
文摘The compartimentation of fluids in the microliter, nanoliter and picoliter range leads recently to many applications of microfluidics in material development, diagnostics and biological screenings. Droplet-based microfluidics allows the improvement of nanoparticle homogeneity and the tuning of particle properties. It supports combinatorial synthesis of inorganic as well as organic substances and can be applied for the cultivation and screening of bacteria, eucaryotic cells and fish embryos. The well-ordered handling and the addressing of microfluid segments improves the information transfer between chemical, biological and electronic systems. Despite this remarkable technical progress, there is a particular importance of microfluidics for future nanotechnological solutions. The hierarchical spatial organization of liquids, particles and gels in microfluidics represents a fundamental biomimetic principle which overcomes the limits of planar technology and opens the gate for realizing complex structured threedimensional nanoarchitectures. Recent applications of microstructured fluids in chemistry and biology and concepts for future developments will be discussed.
基金supported by the National Research Foundation of Korea(NRF)(RS-2024-00343512,RS-2024-00416938).
文摘Sustainable power sources for outdoor wearable electronics are essential for the continuous operation of wearable devices.However,the current lack of engineering design that can harvest energy regardless of weather conditions presents a significant challenge.In this regard,this study introduces a wearable,breathable all-weather usable dual energy harvester(AWuDEH)that can seamlessly generate electrical energy regardless of weather conditions.In this study,the AWuDEH integrated with the thermoelectric generator and the droplet-based electricity generator is demonstrated.The AWuDEH,especially engineered with a bi-functional top substrate for radiative cooling and electrification,achieves sustainable energy harvesting outdoors,thereby addressing the conventional challenge associated with the necessity for separate energy harvesters tailored to outdoor usage contingent on weather conditions.The device reaches a maximum power output of 14.6μW cm^(-2)under simulated sunny conditions and generates a much more enhanced thermoelectric power of 74.78μW cm^(-2)and a droplet-based electric power of 256.25 mW m^(-2)in rainy conditions.As proof,this study developed self-powered wearable electronics capable of acquiring physiological signals in simulated outdoor scenarios.This study presents a promising advancement in wearable technology,offering a potent solution for sustainable energy harvesting independent of weather conditions.
基金supported by the National Natural Science Foundation of China(61971255 and 82111530212)the Natural Science Foundation of Guangdong Province(2021B1515020092)+1 种基金the Shenzhen Science and Technology Innovation Commission(RCYX20200714114736146,WDZC20200821141349001,KCXFZ20201221173207022,KCXFZ20200201101050887)the Shenzhen Bay Laboratory Fund(SZBL2020090501014).
文摘Organoids are expected to function as effective human organ models for precision cancer studies and drug de-velopment.Currently,primary tissue-derived organoids,termed non-engineered organoids(NEOs),are produced by manual pipetting or liquid handling that compromises organoid-organoid homogeneity and organoid-tissue consistency.Droplet-based microfluidics enables automated organoid production with high organoid-organoid homogeneity,organoid-tissue consistency,and a significantly improved production spectrum.It takes advantage of droplet-encapsulation of defined populations of cells and droplet-rendered microstructures that guide cell self-organization.Herein,we studied the droplet-engineered organoids(DEOs),derived from mouse liver tissues and human liver tumors,by using transcriptional analysis and cellular deconvolution on bulk RNA-seq data.The characteristics of DEOs are compared with the parental liver tissues(or tumors)and NEOs.The DEOs are proven higher reproducibility and consistency with the parental tissues,have a high production spectrum and shortened modeling time,and possess inter-organoid homogeneity and inter-tumor cell heterogeneity.
