Hello!My name is Angel.I am an active girl.I am ten years old.I am in Class 1,Grade 5.I want to share my hobbies.First,I like reading books.Books are the ladder of human progress.Reading not only broadens my horizons ...Hello!My name is Angel.I am an active girl.I am ten years old.I am in Class 1,Grade 5.I want to share my hobbies.First,I like reading books.Books are the ladder of human progress.Reading not only broadens my horizons but also fills my heart with wonder and inspires me to dream big.展开更多
One day,my friend asks me what my dream is.I think about it for some days.Then I tell my friend I have many dreams.I want to be a teacher,so I can teach my students all I know like my teachers.I like drawing and if I ...One day,my friend asks me what my dream is.I think about it for some days.Then I tell my friend I have many dreams.I want to be a teacher,so I can teach my students all I know like my teachers.I like drawing and if I can draw what I see,that will be great.I want to travel around the world and see different places.Maybe I can be a writer and write down all the stories on the trip.When I grow up,I go to college and I can be a scientist.I will work hard and make the world better.展开更多
Everyone certainly has a dream city in their heart,whether it is a romantic city like Paris or a fast-paced city like Shanghai.However,my dream city is a place where modernization coexists(共存)with history and nature...Everyone certainly has a dream city in their heart,whether it is a romantic city like Paris or a fast-paced city like Shanghai.However,my dream city is a place where modernization coexists(共存)with history and nature harmoniously.It’s a place that resembles a highly motivated(激励)youth with courage,strong willpower and ambition.Now let me introduce.展开更多
In a small village nestled at the foot of a towering mountain lived a young woman named Maya.She had always been fascinated by the mountains majestic beauty and often dreamed of reaching its summit.Every day,she would...In a small village nestled at the foot of a towering mountain lived a young woman named Maya.She had always been fascinated by the mountains majestic beauty and often dreamed of reaching its summit.Every day,she would look up at the peak,imagining what it would be like to stand on top of the mountain,and see the world from that height.展开更多
"President Xi sent a reply letter to Galai Village!I am so moved and overjoyed for knowing his ongoing concern for us!All our residents of Galai Village are heartfully grateful to him,"said villager Dawa Gya..."President Xi sent a reply letter to Galai Village!I am so moved and overjoyed for knowing his ongoing concern for us!All our residents of Galai Village are heartfully grateful to him,"said villager Dawa Gyaltsen.展开更多
In Jangra Township,Gyantse County,Xigaze City,Xizang,there is a Tibetan girl called Tsering Phentok.Although she cannot walk due to a congenital deformity,she has never given up her love for and pursuit of life.She le...In Jangra Township,Gyantse County,Xigaze City,Xizang,there is a Tibetan girl called Tsering Phentok.Although she cannot walk due to a congenital deformity,she has never given up her love for and pursuit of life.She learned Mandarin and crochet skills by herself through the Internet.展开更多
Chase the Dream of Coral is an inspiring book that shines a light on the brave scientists dedicated to saving our coral reefs.Coral reefs are often called the rainforests of the sea because they support a vast number ...Chase the Dream of Coral is an inspiring book that shines a light on the brave scientists dedicated to saving our coral reefs.Coral reefs are often called the rainforests of the sea because they support a vast number of marine species.However,they are facing serious threats from climate change,pollution and overfishing.This book highlights the urgent need to protect these beautiful and vital ecosystems.展开更多
During Harare’s jacaranda blooming season,the streets are filled with a rich floral fragrance.It was this captivating aroma that first caught the attention of Zhao Ke,then a 28-year-old man,as he paused to take it in...During Harare’s jacaranda blooming season,the streets are filled with a rich floral fragrance.It was this captivating aroma that first caught the attention of Zhao Ke,then a 28-year-old man,as he paused to take it in.Little did he know that this moment would mark the beginning of a nearly three-decade-long connection with Zimbabwe.展开更多
Hi, everyone! I'd like to tell you something about my dream home. It is in a quiet place far away from the city.It's a big house with two floors. On the ground floor, there is a bright kitchen and a dining roo...Hi, everyone! I'd like to tell you something about my dream home. It is in a quiet place far away from the city.It's a big house with two floors. On the ground floor, there is a bright kitchen and a dining room. We usually have nice meal(1) there. On the first floor, there are three bedrooms and a study. It's really nice to have a bedroom of my own.展开更多
目的:构建含下游调控元件的拮抗分子(downstream regulatory element antagonistic modulator,DREAM)基因有效短发夹RNA(shRNA)的慢病毒载体,利用RNA干扰技术抑制DREAM基因表达,开展对神经病理性疼痛基因治疗的实验研究。方法:将靶向大...目的:构建含下游调控元件的拮抗分子(downstream regulatory element antagonistic modulator,DREAM)基因有效短发夹RNA(shRNA)的慢病毒载体,利用RNA干扰技术抑制DREAM基因表达,开展对神经病理性疼痛基因治疗的实验研究。方法:将靶向大鼠DREAM基因的shRNA重组到慢病毒穿梭质粒中,将重组质粒pKCSHR-puro/GFP-DREAM和慢病毒包装系统共转染293T细胞包装成慢病毒,收集病毒上清并进行载体病毒滴度的测定。将纯种健康清洁级成年雄性SD大鼠36只随机分为6组:正常对照组(N)、假手术组(S),根据干预手段的不同将大鼠坐骨神经缩窄损伤(chronic constriction injury of sci-atic nerve,CCI)模型组分为4组:CCI(C0)组,CCI疼痛模型建立后不给任何干预措施,生理盐水对照(C1)组,空白载体对照(C2)组,LV-shRNADREAM慢病毒载体治疗(C3)组。该3组大鼠于CCI后第7天分别于蛛网膜下腔注射生理盐水、空白载体、LV-shRNADREAM慢病毒载体。观察各组大鼠术后3,7,10,14,21 d的疼痛行为学的改变。实验结束取腰段脊髓荧光显微镜检测绿色荧光蛋白(GFP)的表达,Realtime-PCR测定DREAM基因的mRNA含量、Western印迹测定DREAM蛋白含量。结果:测序分析证实载体构建成功,成功进行慢病毒包装,得到病毒液滴度为1.0×108IFU/mL慢病毒载体。鞘内注射LV-shRNADREAM慢病毒载体后,CCI大鼠热痛阈和机械痛阈的异常明显得到改善(P<0.01),腰段脊髓神经细胞DREAM基因mRNA和DREAM蛋白的表达均显著降低(P<0.01)。结论:成功构建了携带大鼠DREAM基因有效shRNA的慢病毒载体,慢病毒携带的短发夹干扰RNA能干扰脊髓中DREAM的表达,为神经病理性疼痛的治疗提供了实验基础。展开更多
目的甘丙肽受体1(galanin receptor 1,GALR1)信号通路在痛觉调节中发挥重要作用,该研究利用坐骨神经慢性压迫(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,探索慢病毒介导的DREAM沉默治疗在CCI模型大鼠疼痛中的作...目的甘丙肽受体1(galanin receptor 1,GALR1)信号通路在痛觉调节中发挥重要作用,该研究利用坐骨神经慢性压迫(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,探索慢病毒介导的DREAM沉默治疗在CCI模型大鼠疼痛中的作用及其对GALR1的表达调控。方法选择健康雄性SD大鼠24只,随机分为4组(n=6):即RNA干扰组、空白载体组、单纯CCI组和正常对照组。鞘内置管前后分别测定基础痛阈,RNA干扰组和空白载体组于CCI后经微导管给予pKCSHR-Puro/GFP-DREAM慢病毒和空白载体,并测定腰段脊髓内下游调控元件拮抗分子(downstream regulatory element antagonist,DREAM)和GALR1的蛋白表达。结果大鼠痛阈的变化:手术同侧热痛阈和机械痛阈测定结果表明,CCI处理后,RNA干扰组、空白载体组及单纯CCI组在各个时间点热痛阈和机械痛阈较正常对照组均显著降低(P<0.01);RNA干扰组鞘内注射后较注射前痛阈显著升高,治疗后相同时间点RNA干扰组较空白载体组及单纯CCI组痛阈亦显著升高。Western blotting结果表明,与其他实验组对比,RNA干扰组的DREAM蛋白表达水平显著下调,而GALR1蛋白表达水平较空白载体组、单纯CCI组亦有显著下调。结论 DREAM可以调控GALR1的表达,提示甘丙肽受体1信号通路参与DREAM基因调节大鼠神经病理性疼痛。展开更多
文摘Hello!My name is Angel.I am an active girl.I am ten years old.I am in Class 1,Grade 5.I want to share my hobbies.First,I like reading books.Books are the ladder of human progress.Reading not only broadens my horizons but also fills my heart with wonder and inspires me to dream big.
文摘One day,my friend asks me what my dream is.I think about it for some days.Then I tell my friend I have many dreams.I want to be a teacher,so I can teach my students all I know like my teachers.I like drawing and if I can draw what I see,that will be great.I want to travel around the world and see different places.Maybe I can be a writer and write down all the stories on the trip.When I grow up,I go to college and I can be a scientist.I will work hard and make the world better.
文摘Everyone certainly has a dream city in their heart,whether it is a romantic city like Paris or a fast-paced city like Shanghai.However,my dream city is a place where modernization coexists(共存)with history and nature harmoniously.It’s a place that resembles a highly motivated(激励)youth with courage,strong willpower and ambition.Now let me introduce.
文摘In a small village nestled at the foot of a towering mountain lived a young woman named Maya.She had always been fascinated by the mountains majestic beauty and often dreamed of reaching its summit.Every day,she would look up at the peak,imagining what it would be like to stand on top of the mountain,and see the world from that height.
文摘"President Xi sent a reply letter to Galai Village!I am so moved and overjoyed for knowing his ongoing concern for us!All our residents of Galai Village are heartfully grateful to him,"said villager Dawa Gyaltsen.
文摘In Jangra Township,Gyantse County,Xigaze City,Xizang,there is a Tibetan girl called Tsering Phentok.Although she cannot walk due to a congenital deformity,she has never given up her love for and pursuit of life.She learned Mandarin and crochet skills by herself through the Internet.
文摘Chase the Dream of Coral is an inspiring book that shines a light on the brave scientists dedicated to saving our coral reefs.Coral reefs are often called the rainforests of the sea because they support a vast number of marine species.However,they are facing serious threats from climate change,pollution and overfishing.This book highlights the urgent need to protect these beautiful and vital ecosystems.
文摘During Harare’s jacaranda blooming season,the streets are filled with a rich floral fragrance.It was this captivating aroma that first caught the attention of Zhao Ke,then a 28-year-old man,as he paused to take it in.Little did he know that this moment would mark the beginning of a nearly three-decade-long connection with Zimbabwe.
文摘Hi, everyone! I'd like to tell you something about my dream home. It is in a quiet place far away from the city.It's a big house with two floors. On the ground floor, there is a bright kitchen and a dining room. We usually have nice meal(1) there. On the first floor, there are three bedrooms and a study. It's really nice to have a bedroom of my own.
文摘目的甘丙肽受体1(galanin receptor 1,GALR1)信号通路在痛觉调节中发挥重要作用,该研究利用坐骨神经慢性压迫(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,探索慢病毒介导的DREAM沉默治疗在CCI模型大鼠疼痛中的作用及其对GALR1的表达调控。方法选择健康雄性SD大鼠24只,随机分为4组(n=6):即RNA干扰组、空白载体组、单纯CCI组和正常对照组。鞘内置管前后分别测定基础痛阈,RNA干扰组和空白载体组于CCI后经微导管给予pKCSHR-Puro/GFP-DREAM慢病毒和空白载体,并测定腰段脊髓内下游调控元件拮抗分子(downstream regulatory element antagonist,DREAM)和GALR1的蛋白表达。结果大鼠痛阈的变化:手术同侧热痛阈和机械痛阈测定结果表明,CCI处理后,RNA干扰组、空白载体组及单纯CCI组在各个时间点热痛阈和机械痛阈较正常对照组均显著降低(P<0.01);RNA干扰组鞘内注射后较注射前痛阈显著升高,治疗后相同时间点RNA干扰组较空白载体组及单纯CCI组痛阈亦显著升高。Western blotting结果表明,与其他实验组对比,RNA干扰组的DREAM蛋白表达水平显著下调,而GALR1蛋白表达水平较空白载体组、单纯CCI组亦有显著下调。结论 DREAM可以调控GALR1的表达,提示甘丙肽受体1信号通路参与DREAM基因调节大鼠神经病理性疼痛。
