期刊文献+
共找到9,367篇文章
< 1 2 250 >
每页显示 20 50 100
DREAM基因在小鼠睾丸不同发育阶段的表达及定位
1
作者 岳续朋 杨瑞 李婷 《黑龙江动物繁殖》 2025年第3期45-49,共5页
为了检测小鼠不同发育阶段睾丸组织中的DREAM基因与蛋白质表达水平及分布,探索其可能参与睾丸的发育和精子发生过程,试验采用Real-time PCR方法检测DREAM基因的表达,利用免疫组织化学法和Western-Blot法检测DREAM蛋白在小鼠不同发育阶... 为了检测小鼠不同发育阶段睾丸组织中的DREAM基因与蛋白质表达水平及分布,探索其可能参与睾丸的发育和精子发生过程,试验采用Real-time PCR方法检测DREAM基因的表达,利用免疫组织化学法和Western-Blot法检测DREAM蛋白在小鼠不同发育阶段睾丸组织内的表达和定位。结果显示:DREAM基因在小鼠睾丸不同发育阶段均有表达;DREAM蛋白主要在精母细胞、间质细胞和精子头部包浆中表达,在精原细胞和圆形精子细胞中少量表达,在基底细胞中不表达;DREAM蛋白在小鼠睾丸发育与精子生成过程中阶段性表达,并且主要定位在精母细胞、间质细胞。 展开更多
关键词 小鼠 睾丸 dream基因 睾丸发育 精子生成
在线阅读 下载PDF
p53S通过DREAM/MMB通路调控Werner综合征细胞端粒DNA复制及延伸
2
作者 杨俊 王倩倩 +1 位作者 姜良霞 罗瑛 《贵州医科大学学报》 2025年第6期781-791,共11页
目的研究抑癌基因TP53(tumor protein 53,TP53)突变后编码的蛋白(p53N236S,p53S)通过下游调节元件拮抗调节剂/髓细胞组织增生病毒癌基因同源物-多细胞B类复合物(DP,RB-like,E2F,and MuvB complex/Myb-MuvB complex,DREAM/MMB)通路对Wer... 目的研究抑癌基因TP53(tumor protein 53,TP53)突变后编码的蛋白(p53N236S,p53S)通过下游调节元件拮抗调节剂/髓细胞组织增生病毒癌基因同源物-多细胞B类复合物(DP,RB-like,E2F,and MuvB complex/Myb-MuvB complex,DREAM/MMB)通路对Werner综合征(werner syndrome,WS)细胞端粒DNA复制的调控作用及其机制。方法以引入p53S突变的第五代WS小鼠胚胎成纤维细胞(G5TM)为研究对象,以野生型(WT)、第五代WS小鼠(G5DM)及敲除p53的第五代WS小鼠(G5TKO)胚胎成纤维细胞为对照,蛋白印记法(Western blot)检测各组细胞中DREAM/MMB相关蛋白和细胞周期相关蛋白的表达,染色质免疫沉淀法(Co-IP)检测各组细胞中DREAM/MMB通路调控关键蛋白与其相互作用蛋白的结合;染色质免疫沉淀法(ChIP)检测三种基因型细胞中(WT、G5DM、G5TM)p53与DAERM/MMB通路基因启动子的结合情况,免疫荧光-荧光原位杂交技术法(immunofluorescence-fluorescence in situ hybridization,IF-FISH)检测各组细胞中DNA解旋酶和端粒保护蛋白以及DNA损伤标志蛋白同端粒信号的共定位情况,端粒DNA实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qPCR)检测各组细胞端粒的相对长度。结果Western blot结果表明,在G5TM细胞中p53S蛋白表达显著上调,下游靶基因p21在G5TM及G5KO细胞中表达被抑制(P<0.05);通过对比WT、G5DM及G5TKO细胞,发现p53S特异性激活DREAM/MMB复合体通路,关键组分B-Myb、FoxM1、p130、p-Rb、Lin54、Lin9和E2f1的表达均被上调(P<0.05);Co-IP实验揭示,p53S通过增强Lin9与p130及B-Myb的相互作用,促进DREAM/MMB复合体的功能重构;ChIP分析证实,p53能直接结合Lin54、Lin9、Mybl2和Rbl2的启动子区域,其结合强度高于WT及G5DM,提示其通过转录调控激活这些基因(P<0.05),同时p53S上调Cdc25c及CDK1/2/4/6等蛋白的表达,协同推动细胞周期进程(P<0.05);IF-FISH结果显示,p53S促进Recql4、Blm、Terf1和Pot1a与端粒DNA的共定位,并增强端粒信号强度(P<0.05),而G5DM及G5TKO细胞中ATR在端粒区异常聚集,表明p53S通过招募保护因子维持端粒完整性;端粒qPCR证实p53S可恢复端粒复制能力(P<0.05),提示其通过激活增殖通路与端粒保护机制协同促进细胞端粒的延伸。结论p53S的引入可以上调DNA解旋酶、端粒保护蛋白并招募到端粒DNA,缓解WS所造成的端粒复制压力,有利于端粒的延伸。 展开更多
关键词 WERNER综合征 p53S突变 dream/MMB DNA解旋酶 端粒
暂未订购
My Hobbies and Dream Job
3
作者 熊安琪 鲍倩晴(指导) 《疯狂英语(双语世界)》 2025年第1期75-75,共1页
Hello!My name is Angel.I am an active girl.I am ten years old.I am in Class 1,Grade 5.I want to share my hobbies.First,I like reading books.Books are the ladder of human progress.Reading not only broadens my horizons ... Hello!My name is Angel.I am an active girl.I am ten years old.I am in Class 1,Grade 5.I want to share my hobbies.First,I like reading books.Books are the ladder of human progress.Reading not only broadens my horizons but also fills my heart with wonder and inspires me to dream big. 展开更多
关键词 BOOKS hobbies reading dreamS INSPIRATION HORIZONS
在线阅读 下载PDF
My Dream
4
作者 刘晶晶 曹娟(指导) 《中学生英语》 2025年第9期7-7,共1页
One day,my friend asks me what my dream is.I think about it for some days.Then I tell my friend I have many dreams.I want to be a teacher,so I can teach my students all I know like my teachers.I like drawing and if I ... One day,my friend asks me what my dream is.I think about it for some days.Then I tell my friend I have many dreams.I want to be a teacher,so I can teach my students all I know like my teachers.I like drawing and if I can draw what I see,that will be great.I want to travel around the world and see different places.Maybe I can be a writer and write down all the stories on the trip.When I grow up,I go to college and I can be a scientist.I will work hard and make the world better. 展开更多
关键词 DRAWING teaching TRAVELING dreamS COLLEGE WRITING science world improvement
在线阅读 下载PDF
My dream city
5
作者 王歆源 于晓月(指导) 《疯狂英语(初中天地)》 2025年第4期71-71,共1页
Everyone certainly has a dream city in their heart,whether it is a romantic city like Paris or a fast-paced city like Shanghai.However,my dream city is a place where modernization coexists(共存)with history and nature... Everyone certainly has a dream city in their heart,whether it is a romantic city like Paris or a fast-paced city like Shanghai.However,my dream city is a place where modernization coexists(共存)with history and nature harmoniously.It’s a place that resembles a highly motivated(激励)youth with courage,strong willpower and ambition.Now let me introduce. 展开更多
关键词 history motivation dream city WILLPOWER COURAGE AMBITION modernization HARMONY
在线阅读 下载PDF
Maya's mountain dream
6
作者 粟榜华 《疯狂英语(新读写)》 2025年第2期50-51,76,77,共4页
In a small village nestled at the foot of a towering mountain lived a young woman named Maya.She had always been fascinated by the mountains majestic beauty and often dreamed of reaching its summit.Every day,she would... In a small village nestled at the foot of a towering mountain lived a young woman named Maya.She had always been fascinated by the mountains majestic beauty and often dreamed of reaching its summit.Every day,she would look up at the peak,imagining what it would be like to stand on top of the mountain,and see the world from that height. 展开更多
关键词 MOUNTAIN dream SUMMIT HEIGHT VILLAGE
在线阅读 下载PDF
Chasing Dreams Under the Guidance of the Big Dipper
7
作者 Wang Xi 《China's Tibet》 2025年第4期70-70,共1页
"President Xi sent a reply letter to Galai Village!I am so moved and overjoyed for knowing his ongoing concern for us!All our residents of Galai Village are heartfully grateful to him,"said villager Dawa Gya... "President Xi sent a reply letter to Galai Village!I am so moved and overjoyed for knowing his ongoing concern for us!All our residents of Galai Village are heartfully grateful to him,"said villager Dawa Gyaltsen. 展开更多
关键词 Galai Village Letter GRATITUDE Xi Beidou Star dream Chasing
在线阅读 下载PDF
FROM GYANTSE TO THE WORLD CROCHETING COLORFUL DREAM WITH HANDS
8
作者 Wu Shanyan 《China's Tibet》 2025年第1期20-23,共4页
In Jangra Township,Gyantse County,Xigaze City,Xizang,there is a Tibetan girl called Tsering Phentok.Although she cannot walk due to a congenital deformity,she has never given up her love for and pursuit of life.She le... In Jangra Township,Gyantse County,Xigaze City,Xizang,there is a Tibetan girl called Tsering Phentok.Although she cannot walk due to a congenital deformity,she has never given up her love for and pursuit of life.She learned Mandarin and crochet skills by herself through the Internet. 展开更多
关键词 dream PURSUIT TIBETAN
在线阅读 下载PDF
Chase the Dream of Coral
9
作者 孙朝岚 《疯狂英语(新读写)》 2025年第8期18-20,76,共4页
Chase the Dream of Coral is an inspiring book that shines a light on the brave scientists dedicated to saving our coral reefs.Coral reefs are often called the rainforests of the sea because they support a vast number ... Chase the Dream of Coral is an inspiring book that shines a light on the brave scientists dedicated to saving our coral reefs.Coral reefs are often called the rainforests of the sea because they support a vast number of marine species.However,they are facing serious threats from climate change,pollution and overfishing.This book highlights the urgent need to protect these beautiful and vital ecosystems. 展开更多
关键词 ecosystem protection coral reefs climate change chase dream coral OVERFISHING POLLUTION
在线阅读 下载PDF
Bringing Dreams to Life A Chinese entrepreneur helps young people to realise their dreams in Zimbabwe
10
作者 XIA YUANYUAN 《ChinAfrica》 2025年第3期56-57,共2页
During Harare’s jacaranda blooming season,the streets are filled with a rich floral fragrance.It was this captivating aroma that first caught the attention of Zhao Ke,then a 28-year-old man,as he paused to take it in... During Harare’s jacaranda blooming season,the streets are filled with a rich floral fragrance.It was this captivating aroma that first caught the attention of Zhao Ke,then a 28-year-old man,as he paused to take it in.Little did he know that this moment would mark the beginning of a nearly three-decade-long connection with Zimbabwe. 展开更多
关键词 dreamS FILLED SEASON
原文传递
My Dream Home
11
作者 吴启豪 《中学生英语》 2025年第21期6-6,共1页
Hi, everyone! I'd like to tell you something about my dream home. It is in a quiet place far away from the city.It's a big house with two floors. On the ground floor, there is a bright kitchen and a dining roo... Hi, everyone! I'd like to tell you something about my dream home. It is in a quiet place far away from the city.It's a big house with two floors. On the ground floor, there is a bright kitchen and a dining room. We usually have nice meal(1) there. On the first floor, there are three bedrooms and a study. It's really nice to have a bedroom of my own. 展开更多
关键词 quiet place BEDROOMS dining room big house KITCHEN dream home first floor ground floor
在线阅读 下载PDF
基于DREAM算法的DSGE模型参数估计精度研究
12
作者 罗琦 赵胜民 《统计与决策》 CSSCI 北大核心 2024年第2期46-51,共6页
文章将人工智能算法中的DREAM算法首次应用到动态随机一般均衡模型的参数估计中,并以动态随机一般均衡模型LS(2005)为例对该算法的估计精度进行了系统分析,研究结果表明:根据待估参数随机抽样序列的箱线图来看,由DREAM算法产生的待估参... 文章将人工智能算法中的DREAM算法首次应用到动态随机一般均衡模型的参数估计中,并以动态随机一般均衡模型LS(2005)为例对该算法的估计精度进行了系统分析,研究结果表明:根据待估参数随机抽样序列的箱线图来看,由DREAM算法产生的待估参数随机抽样序列的箱体长度均比RWMH和IMH算法产生的箱体长度要长,说明由DREAM算法产生的参数估计序列的分散程度比RWMH和IMH算法要大,表明了DREAM算法遍历参数空间范围更为广泛,算法逃逸局部最优值的能力更强。另外,从箱线图中的中位数数值来看,除了5个参数以外,由DREAM算法产生的参数估计序列的中位数相比RWMH和IMH算法,与真实数据生成过程更为接近,说明由DREAM算法产生的参数估计值大部分都集中在参数的真值附近。由于DREAM算法依据IQR统计方法除去无用链,故由DREAM算法产生的参数估计序列的异常值也明显降低,而RWMH算法产生的参数估计序列的异常值尤其多。从待估参数的90%最大后验密度可信区间来看,DREAM算法产生的待估参数90%最大后验密度可信区间除了3个参数以外,其余全部包含了真值,而传统的RWMH和IMH算法分别只有7个和1个区间包含了真值,表明DREAM算法的估计不确定性远小于传统的RWMH和IMH算法。最后,根据待估参数的无效因子来看,DREAM算法产生的待估参数序列与传统的RWMH和IMH算法相比,其相关性更弱,即无效因子数值更小,这一点进一步验证了DREAM算法遍历整个参数空间的能力更强。 展开更多
关键词 dream算法 DSGE模型 估计精度
在线阅读 下载PDF
Dreaming across Languages and Cultures
13
作者 ZHOU LIN 《China Today》 2024年第11期64-66,共3页
From a Ph.D.student to a budding sinologist,a young Spanish woman explains her vision for more cultural exchanges between China and her country.DREAM of the Red Chamber,Cao Xueqin’s classic about the rise and decline... From a Ph.D.student to a budding sinologist,a young Spanish woman explains her vision for more cultural exchanges between China and her country.DREAM of the Red Chamber,Cao Xueqin’s classic about the rise and decline of a family that is also a portrayal of its contemporary Qing Dynasty(1644-1911),continues to inspire and influence long after it was published in the 18th century.Patricia Amate Núñez is a current acolyte. 展开更多
关键词 dream CONTINUE EXCHANGES
在线阅读 下载PDF
The Application of Embodied Cognitive Linguistics in the English Translation of Two-Part Allegorical Saying—An Analysis of Examples From A Dream of Red Mansions
14
作者 HE Jiamei ZOU Jianling 《Sino-US English Teaching》 2024年第7期337-342,共6页
As a kind of widely circulated folk language,two-part allegorical saying(“xiehouyu”)carries the wisdom of the Chinese people and is characterized with humor and vivid imagery.Taking the two English translation versi... As a kind of widely circulated folk language,two-part allegorical saying(“xiehouyu”)carries the wisdom of the Chinese people and is characterized with humor and vivid imagery.Taking the two English translation versions of A Dream of Red Mansions as examples,this paper analyzes the English translation of two-part allegorical saying based on the core principle of Embodied Cognitive Linguistics(ECL),“Reality-Cognition-Language”,and explores how the English translation of two-part allegorical saying reflects the three dimensions of ECL. 展开更多
关键词 two-part allegorical saying A dream in Red Mansions Embodied Cognitive Linguistics TRANSLATION
在线阅读 下载PDF
Translation Studies from a Cross-Cultural Perspective:Reinventing Subjectivity-A Case Study of English Translation of A Dream of Red Mansions
15
作者 YANG Yin-chuan YANG Zhan 《Journal of Literature and Art Studies》 2024年第4期279-283,共5页
The integration of cross-cultural inquiries into the realm of translation has given rise to a distinct subfield known as cross-cultural translation studies.This approach emphasizes the importance of delving into the c... The integration of cross-cultural inquiries into the realm of translation has given rise to a distinct subfield known as cross-cultural translation studies.This approach emphasizes the importance of delving into the cultural subtleties and the awareness of cultural subjectivity within the translation process.Central to this framework is the concept of reshaping cultural nuances and the subjectivity of culture.This paper introduces three principal translation strategies that emerge from a cross-cultural standpoint:the alienation translation strategy,the‘cannibalism’translation strategy,and the destructive translation strategy.Utilizing the English translation of“Dream of the Red Chamber”as a case study,the paper examines how cultural nuances and cultural subjectivity influence the selection of translation strategies and demonstrates the practical application of cross-cultural research within the translation sphere.Hence,the significance of cross-cultural translation studies lies in its capacity to offer a novel lens through which to view translation studies.It encourages a reevaluation of cultural nuances and subjectivity in translation,which is of profound practical importance for advancing the field of translation studies,enhancing cross-cultural communication,and facilitating the spread of Chinese discourse on the global stage. 展开更多
关键词 CROSS-CULTURAL translation strategies A dream of Red Mansions
在线阅读 下载PDF
My Dream of Becoming a Good Teacher
16
作者 任怡敏 陈需亮(指导) 《中学生英语》 2024年第42期7-7,共1页
I want to be a teacher because I love students very much.And I am eager to make my students learn more knowledge.In my opinion,the teacher is the greatest job.I can teach students how to study,how to be a person who p... I want to be a teacher because I love students very much.And I am eager to make my students learn more knowledge.In my opinion,the teacher is the greatest job.I can teach students how to study,how to be a person who possesses the good manners and so on. 展开更多
关键词 And dream KNOWLEDGE
在线阅读 下载PDF
Living the Mechanical Dream
17
作者 KEDIR ABDUREZAK NURIYE 《ChinAfrica》 2024年第3期56-56,共1页
Studying in China is not just about academics,it is also about connecting with communities and learning local culture.As a child,I was captivated by machines and their inner workings.I yearned to design contraptions t... Studying in China is not just about academics,it is also about connecting with communities and learning local culture.As a child,I was captivated by machines and their inner workings.I yearned to design contraptions that could solve real-world problems and improve people’s lives.So,when it came time to choose a university,I knew that mechanical engineering was the path for me. 展开更多
关键词 earned connecting dream
原文传递
Fascination For Planes Young Ghanaian pursues her aviation dream in China
18
作者 GE LIJUN 《ChinAfrica》 2024年第5期54-55,共2页
Every morning at 7 o’clock,Rachael Amoah,a young woman from Ghana,prepares for a new day of challenges and innovations at a leading aerospace company in Shanghai’s Pudong New Area.In March last year,she completed he... Every morning at 7 o’clock,Rachael Amoah,a young woman from Ghana,prepares for a new day of challenges and innovations at a leading aerospace company in Shanghai’s Pudong New Area.In March last year,she completed her studies in the School of Aeronautics and Astronautics at Shanghai Jiao Tong University(SJTU),and fulfilled her ambition to work in this megalopolis. 展开更多
关键词 AVIATION dream pursue
原文传递
Dreaming Back to Xi’an
19
作者 黄祺钦 金华(指导) 《疯狂英语(双语世界)》 2024年第4期74-75,共2页
It was a sunny day in August when my parents asked my brother and me where we would like to visit.After a lively discussion,we all agreed on Xi’an,one of the most famous ancient cities in China.
关键词 SUNNY dream China.
在线阅读 下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部