Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corres...Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corresponding gene targets of each drug in Gan Dou Ling were obtained by using TCMSP database.The database of gene mutation and disease related gene was searched through the GeneCards database,DrugBank database,PharmGKB database and the DisGeNET database.After the intersection of drug and disease target genes.The STRING website was used to analyze the protein-protein interaction degree of target genes,and import the data to Cytoscape software 38.2 to analyze protein interaction network.The GO databases and KEGG databases were obtained in R language for enrichment analysis.On this basis,Masson staining were used to observe the degree of liver fibrosis in copper loaded mouse model,and the results of network pharmacological analysis were verified by Western Blot(WB).Results:A total of 108 drug disease intersection genes were analyzed by network pharmacology.Through PPI network analysis,JUN was found to be the key genes.The enrichment analysis of KEGG pathway showed that MAPK signal pathway was the important potential target pathways.Animal experiments showed that Gan Dou Ling could reduce liver fibrosis and inhibit the phosphorylation of P38,JNK and C-JUN in copper loaded mice.Conclusion:Gan Dou Ling may achieve the effect of treating WD liver fibrosis by inhibiting P38/JNK signaling pathway.展开更多
THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food ...THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food made from fermented and salted black beans,which is commonly used as a condiment or seasoning for a variety of dishes.The history of Huangyao’s Douchi processing techniques can be traced back to the late Yuan Dynasty(1271-1368)and the early Ming Dynasty(1368-1644).According to a historical record of the Qing Dynasty(1644-1911),“Huangyao is famous for its Douchi and has no rivals.”展开更多
At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper...At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper stage and the satellites.The launch opens up a new era for the global networking of the Bei Dou Navigation Satellite system(Bei Dou system),which is the final step of the"three-step development strategy"of the system.展开更多
基金supported by the National Natural Science Foundation of China(No.81973825)the Natural Science Research Foundation of Anhui Province Universities(No.KJ2020A0436)。
文摘Objective:To explore and verify the mechanism of Gan Dou Ling in improving liver fibrosis in Wilson disease(WD)by network pharmacology and copper loaded mice experiments.Methods:The main chemical components and corresponding gene targets of each drug in Gan Dou Ling were obtained by using TCMSP database.The database of gene mutation and disease related gene was searched through the GeneCards database,DrugBank database,PharmGKB database and the DisGeNET database.After the intersection of drug and disease target genes.The STRING website was used to analyze the protein-protein interaction degree of target genes,and import the data to Cytoscape software 38.2 to analyze protein interaction network.The GO databases and KEGG databases were obtained in R language for enrichment analysis.On this basis,Masson staining were used to observe the degree of liver fibrosis in copper loaded mouse model,and the results of network pharmacological analysis were verified by Western Blot(WB).Results:A total of 108 drug disease intersection genes were analyzed by network pharmacology.Through PPI network analysis,JUN was found to be the key genes.The enrichment analysis of KEGG pathway showed that MAPK signal pathway was the important potential target pathways.Animal experiments showed that Gan Dou Ling could reduce liver fibrosis and inhibit the phosphorylation of P38,JNK and C-JUN in copper loaded mice.Conclusion:Gan Dou Ling may achieve the effect of treating WD liver fibrosis by inhibiting P38/JNK signaling pathway.
文摘THE ancient town of Huangyao located in Zhaoping County,Hezhou City,south China’s Guangxi Zhuang Autonomous Region,is renown for its time-honored Douchi processing techniques.Douchi is a kind of popular Chinese food made from fermented and salted black beans,which is commonly used as a condiment or seasoning for a variety of dishes.The history of Huangyao’s Douchi processing techniques can be traced back to the late Yuan Dynasty(1271-1368)and the early Ming Dynasty(1368-1644).According to a historical record of the Qing Dynasty(1644-1911),“Huangyao is famous for its Douchi and has no rivals.”
文摘At 19:45 Beijing time on November 5,a LM-3B/Yuanzheng 1 launch vehicle lifted off from the Xichang Satellite Launch Center,putting the first two Bei Dou 3 satellites into orbit after the separation of the rocket upper stage and the satellites.The launch opens up a new era for the global networking of the Bei Dou Navigation Satellite system(Bei Dou system),which is the final step of the"three-step development strategy"of the system.
