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Development of surface displaying system for heterologous protein expression in Candida tropicalis 被引量:1
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作者 Tingting Wang Haibing Zhang +5 位作者 Mengxuan Li Jiaxi Zheng Yuanyuan Xia Haiquan Yang Wei Shen Xianzhong Chen 《Systems Microbiology and Biomanufacturing》 2023年第2期298-311,共14页
The purpose of this study was to assess the potential application of cell surface display in Candida tropicalis.Surface display gene cassettes were constructed using five anchoring proteins from Saccharomyces cerevisi... The purpose of this study was to assess the potential application of cell surface display in Candida tropicalis.Surface display gene cassettes were constructed using five anchoring proteins from Saccharomyces cerevisiae,three of which[(suppression of exponential defect protein,SED1),(cell wall protein 2,CWP2)and(delayed anaerobic protein 4,DAN4)]were reported to show higher activity of heterologous proteins thanα-agglutinin(AGα1).The performance of yeast-enhanced green fluorescent protein(yeGFP)was evaluated using laser scanning confocal microscopy and flow cytometry.The results showed that the three anchoring regions(SED1,CWP2 and AGα1)successfully displayed yeGFP on the cell wall.To investigate the effect of the three anchoring proteins on the surface display of Rhizopus oryzaeα-amylase(ROA1)and Aspergillus aculeatusβ-glucosidase(BGL1)in C.tropicalis,we constructed surface display gene cassettes for ROA1 and BGL1,respectively.The strains containing the anchoring proteins SED1 and CWP2 showed higher activity of ROA1 and BGL1 than the strains containing the anchoring protein AGα1.The highest ROA1 and BGL1 activities of strains with SED1 were 6.37 U/g CDW and 7.93 U/g CDW,respectively,which were sixfold and eightfold higher than those of strain with AGα1.In addition,we also optimized signal peptides.The results indicated that signal peptides have an impact on enzyme activity. 展开更多
关键词 Yeast surface display Anchoring protein Glycosylphosphatidylinositol(GPI) Α-AMYLASE Β-GLUCOSIDASE Whole cell catalysis
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NOVEL VOLUMETRIC THREE-DIMENSIONAL DISPLAY SYSTEM 被引量:2
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作者 李莉 龚华军 +1 位作者 沈春林 谭皓 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2008年第1期32-36,共5页
A novel volumetric three-dimensional(3-D) display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system de... A novel volumetric three-dimensional(3-D) display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system design is introduced and key components are described. Experimental results show that the 3-D system can guide people freely walk around the display to inspect the true 3-D image without goggles. 展开更多
关键词 display devices model buildings 3-D volumetric display eye persistence
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A robot simulation system with virtual force display and feedback based on master manipulator 被引量:2
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作者 祝学云 吴涓 《Journal of Southeast University(English Edition)》 EI CAS 2003年第1期70-73,共4页
This paper describes a virtual environment, which can present dynamic force transformation during the control of objects. A 5-DOF haptic interface with the capability to generate kinesthetic effect is combined. In thi... This paper describes a virtual environment, which can present dynamic force transformation during the control of objects. A 5-DOF haptic interface with the capability to generate kinesthetic effect is combined. In this system, the operator manipulates an object in a virtual environment by using the 5-DOF master arm. When contacting with the virtual object, the contact force can be calculated and shown in the graphic interface. The contact response and deformation of the virtual object, which are usually called haptic rendering, also can be performed. The study supplies an approach to improve the operator’s immersion and can be used in many tele-robot control fields. 展开更多
关键词 virtual reality haptic display contact detection contact response
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VOLUMETRIC-SWEPT DISPLAY SYSTEM BASED ON HELIX ROTATING SCREEN AND DMD
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作者 岳键 赵晓庚 +2 位作者 邢建芳 潘文平 沈春林 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2011年第4期367-371,共5页
Based on the helix rotating screen and the digital micro-mirror device (DMD), the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an ... Based on the helix rotating screen and the digital micro-mirror device (DMD), the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular (SLM) technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system. 展开更多
关键词 true 3-D display volumetric 3-D display volumetric-swept display helix rotating screen digital micro-mirror device (DMD)
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PHOTO-ELECTRIO MEASURING SYSTEM FOR TOOL EDGE DETECTION BY THE TEOHNOLOGY OF COMPUTER DYNAMIO DISPLAY MOVING IMAGE
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作者 孙旭东 林玉池 张国雄 《Transactions of Tianjin University》 EI CAS 1999年第1期47-51,共5页
This paper describes a technology of dynamic display moving image by computer monitor,which is initially used in the design of tool detection system. The paper presents the hardware and software principie and edge det... This paper describes a technology of dynamic display moving image by computer monitor,which is initially used in the design of tool detection system. The paper presents the hardware and software principie and edge detection process. The way of marking edge point coordinates and stability of moving image also is analyzed. The method reforms the conventional design of the 2-D vision detection system. Moreover,it facilitates the design of the systematic mechanical construction,is convenient to compile instrument systemsoftware,and realizes to detect and track display image simultaneously. By the work,the tool detection system is improved to practical application. 展开更多
关键词 edge detection dynamic display tool detection instrument
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Head-mounted display-based intuitive virtual reality training system for the mining industry 被引量:12
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作者 Zhang Hui 《International Journal of Mining Science and Technology》 SCIE EI CSCD 2017年第4期717-722,共6页
Virtual reality(VR) training technology in the mining industry is a new field of research and utilization.The successful application of VR training system is critical to mine safety and production. Through the statist... Virtual reality(VR) training technology in the mining industry is a new field of research and utilization.The successful application of VR training system is critical to mine safety and production. Through the statistics of the current research and applications of VR training systems in mining industry, all the input/output devices are classified. Based on the classifications of the input/output devices that are used in the VR system, the current VR training systems for the mining industry could be divided into three types: screen-based general type, projector-based customized type, and head-mounted display(HMD)-based intuitive type. By employing a VR headset, a smartphone and a leap motion device, an HMDbased intuitive type VR training system prototype for drilling in underground mines has been developed.Ten trainees tried both the HMD-based intuitive system and the screen-based general control system to compare the experiences and training effects. The results show that the HMD-based system can give a much better user experience and is easy to use. Three of the five components of a VR training system,namely, the user, the tasks, and software and database should be given more attention in future research.With more available technologies of input and output devices, VR engines, and system software, the VR training system will eventually yield much better training results, and will play a more important role in as a training tool for mine safety. 展开更多
关键词 Virtual reality TRAINING Head-mounted display High immersive Intuitive Mining industry
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Floral display and breeding system of Jatropha curcas L. 被引量:6
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作者 Luo Chang-wei Li Kun +1 位作者 Chen You Sun Yong-yu 《Forestry Studies in China》 CAS 2007年第2期114-119,共6页
Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. In this paper, we studied the reproduction characteristics of Jatropha curcas in Yuanjiang County ... Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. In this paper, we studied the reproduction characteristics of Jatropha curcas in Yuanjiang County (23°36'1'4, 101°00'E), Yunnan Province. The plant produces flowers in dichasial inflorescences. Normally, the flowers are unisexual, and male and female flowers are produced in the same inflorescence. Only a few male flowers are produced in an inflorescence, and fruits are produced only through pollination between different flowers from the same or different plants. By the treatments of emasculation, bagging and artificial pollination in this experiment, there were few but same fruit set ratios when the inflorescences were emasculated, bagged, or bagged with net, except artificial pollination treatments, which showed that Jatropha curcas could produce fruit through apomixis but not wind pollination. When the inflorescences were unbagged, unemasculated and with free pollination treatments, or bagged, emasculated and with artificial cross-pollination treatments, or unbagged, emasculated and with free pollination treatments, there were many fruits produced. It showed that Jatropha curcas shows outcrossing, is self-compatible, and demanding for pollinators. Normally, the male flowers open first and a few flowers bloom in one day in a raceme. These flowers last a long time in bloom. However, a large number of female flowers open from the third to the fifth day, with some female flowers opening first in a few raceme. This shows a tendency to promote xenogamy and minimize geitonogamy. 展开更多
关键词 Jatropha curcas floral display breeding system outcrossing index pollen-ovule ratio
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Electronic Chart Display and Information System 被引量:3
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作者 WAN Xiaoxia GAN Chaohua 《Geo-Spatial Information Science》 2002年第1期7-11,21,共6页
This paper introduces the most advanced form of electronic chart display and information systems (ECDIS) which complies with the Performance Standards for ECDIS established by the International Maritime Organization (... This paper introduces the most advanced form of electronic chart display and information systems (ECDIS) which complies with the Performance Standards for ECDIS established by the International Maritime Organization (IMO) and the International Hydrographic Organization (IHO).It also describes the key features and the important functions of ECDIS.Then the future development of ECDIS in marine is discussed. 展开更多
关键词 ECDIS ECS DISPLAY key features performance standards
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SimWall:a practical user-friendly stereo tiled display wall system 被引量:2
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作者 XIE Li-jun ZHENG Yao +2 位作者 YANG Ting-jun GAO Wen-xuan PAN Ning-he 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第4期596-604,共9页
SimWall is a user-friendly, stereo tiled display wall system composed of 18 commodity projectors operated by a Linux graphics cluster. Collaborating together, these projectors work as a single logical display capable ... SimWall is a user-friendly, stereo tiled display wall system composed of 18 commodity projectors operated by a Linux graphics cluster. Collaborating together, these projectors work as a single logical display capable of giving a high-resolution show, large-scale, and passive stereo scene. In order to avoid tedious system setup and maintenance, software-based automatic geometry and photometric calibration are used. The software calibration is integrated to the system seamlessly by an on-card transform method and is transparent to users. To end-users, SimWall works just as a common PC, but provides super computing, rendering and displaying ability. In addition, SimWall has stereoscopic function that gives users a semi-immersive experience in polarized passive way. This paper presents system architecture, implementation, and other technical issues such as hardware constraints, projectors alignment, geometry and photometric calibration, implementation of passive stereo, and development of overall soft- ware environment. 展开更多
关键词 Tiled display wall Stereoscopic display Multi-projectors display Parallel rendering Camera-based geometry and photometric calibration
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Design of a titl angle measurement system based on ADXL345 sensor 被引量:7
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作者 SU Yan-ping GONG Mao-fa AN Bin ZHANG Jian-yu ZHAO Xu-jie 《Journal of Measurement Science and Instrumentation》 CAS 2014年第2期19-22,共4页
The titl angle measurement system based on the ADXL345 of three-axis acceleration sensor is introduced, and the composition and working principle of the system are described. The MSP430F149 microcontroller of ultra-lo... The titl angle measurement system based on the ADXL345 of three-axis acceleration sensor is introduced, and the composition and working principle of the system are described. The MSP430F149 microcontroller of ultra-low power and high performance is adopted. Firstly, the voltage value which is proportional to the acceleration value is output by ADXL345 when the system is powered. Secondly, the voltage value is converted into the corresponding digital signal by AD converter of the sensor. Thirdly, the digital signal is transformed into the titl angle according to the arithmetic processing of the MSP430F149. Finally, the corresponding angle is displayed on the liquid crystal display (LCD) screen. Experimental results show that the design has high sensitivity and response speed compared with the conventional methods. It is suitable for practical engineering application because of simple structure, low cost and low power consumption. 展开更多
关键词 ADXL345 MSP430F149 tilt angle measurement liquid crystal display (LCD)
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Consensus intuitionistic fuzzy group decision-making method for aircraft cockpit display and control system evaluation 被引量:6
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作者 Tao Geng An Zhang Guangshan Lu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2013年第4期634-641,共8页
A novel group decision-making (GDM) method based on intuitionistic fuzzy sets (IFSs) is developed to evaluate the ergonomics of aircraft cockpit display and control system (ACDCS). The GDM process with four step... A novel group decision-making (GDM) method based on intuitionistic fuzzy sets (IFSs) is developed to evaluate the ergonomics of aircraft cockpit display and control system (ACDCS). The GDM process with four steps is discussed. Firstly, approaches are proposed to transform four types of common judgement representations into a unified expression by the form of the IFS, and the features of unifications are analyzed. Then, the aggregation operator called the IFSs weighted averaging (IFSWA) operator is taken to synthesize decision-makers’ (DMs’) preferences by the form of the IFS. In this operator, the DM’s reliability weights factors are determined based on the distance measure between their preferences. Finally, an improved score function is used to rank alternatives and to get the best one. An illustrative example proves the proposed method is effective to valuate the ergonomics of the ACDCS. 展开更多
关键词 aircraft cockpit display and control system (ACDCS) group decision-making (GDM) intuitionistic fuzzy set (IFS) different preference representation weighted averaging (WA) operator.
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Performance Enhancements in the EAST Digital Plasma Control System 被引量:2
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作者 袁旗平 肖炳甲 +1 位作者 B.PENAFLOR 刘连忠 《Plasma Science and Technology》 SCIE EI CAS CSCD 2009年第6期734-738,共5页
The EAST plasma control system (PCS) is in continuous development to satisfy the EAST experimental requirements. In order to realize low latency and distortion-free signal transmission between PCS and servo systems ... The EAST plasma control system (PCS) is in continuous development to satisfy the EAST experimental requirements. In order to realize low latency and distortion-free signal transmission between PCS and servo systems such as the poloidal field power supply, in-vessel coil power supply and real-time scope, reflective memory boards (RFM) were applied. The new hardware layout and enhanced performance are reported. Newly implemented PCS control algorithms for gas control and real-time data display are also presented. 展开更多
关键词 PCS reflective memory board data transmission gas control real-time display
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An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System 被引量:1
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作者 Danlei Liu Haoran Geng +4 位作者 Zilei Zhang Yifan Xing Danlu Yang Zhicheng Liu Dapeng Wang 《Virologica Sinica》 SCIE CAS CSCD 2020年第1期103-109,共7页
Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors... Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens(HBGAs). Previously, the protruding domain protein(P protein) of human norovirus(genotype Ⅱ.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV(G9 P[8]) in this study. In the system, VP8*could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin(PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix. 展开更多
关键词 ROTAVIRUS BACTERIAL SURFACE display system RECEPTORS Histo-blood group antigens(HBGAs) SALIVA
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A robotic tele-drill system over network using predictive display 被引量:2
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作者 谢小辉 Sun Lining Du Zhijiang Cai Hegao 《High Technology Letters》 EI CAS 2006年第1期42-46,共5页
Based on a robotic telesurgery system whose function is to liberate doctor from X-ray radiation, a robotic tele-drill system is constructed. The system is in client/server structure. Client part includes main control ... Based on a robotic telesurgery system whose function is to liberate doctor from X-ray radiation, a robotic tele-drill system is constructed. The system is in client/server structure. Client part includes main control interface, video-audio interface and predictive display interface. Server part includes robot control server and video, audio server. For applying to teleoperation, a virtual reality environment of the system developed by using Java, Java 3D, Pro/E, etc. is established. The geometry and kinematics model of serial robot MOTOMAN sv3x, parallel robot, C-type arm and X-ray machine, surgery bed and its work environment are fulfilled in it. Simulation engine and its simulation syntax are finished, which made the environment controllable. This environment is used as predictive display interface in the telerobotics in order to tackling the problem in visualization feedback as ambiguous or time delay. Experiments that verified feasibility of the system have been done. 展开更多
关键词 robotic teleoperation virtual reality predictive display Java 3D
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Low-Cost Tiled Display System Architecture with Dynamic Web-Based Management 被引量:1
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作者 Daniel Marfil Fernando Boronat +1 位作者 F.Javier Pastor Anna Vidal 《China Communications》 SCIE CSCD 2022年第10期143-168,共26页
In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configur... In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configuration service are proposed. It has been designed to support both stored and live broadcast/broadband content, in mosaic or warp distributions. The displays and devices can be dynamically configured via web in different ways: the displays can create a single display of a larger size;or they can be configured in a customized way in order to playout different media contents in different display combinations. As display renderers, low-cost devices are proposed as the main hardware element to obtain affordable videowall systems. As a proof of concept, two prototypes have been implemented, including an accurate synchronization mechanism based on a Master/Slave control scheme and aggressive and smooth playout adjustment techniques. To evidence the good performance of the prototypes and configuration service, both objective and subjective evaluations have been conducted regarding synchronization accuracy and usability. On the one hand, the mean values of the asynchronies between the video playout processes in each display are kept below 25ms (i.e., frame accuracy). On the other hand, the obtained usability score in the System Usability Scale (SUS) test has been 88.65, which is considered as excellent. 展开更多
关键词 AMP media synchronization MULTI-SCREEN playout adjustment tiled displays videowall
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Natural selection on floral traits of Caltha scaposa (Ranunculaceae), an alpine perennial with generalized pollination system from Northwest Yunnan 被引量:1
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作者 Guopeng Zhang Lihua Meng +4 位作者 Zhikun Wu Zhiqiang Zhang Lingjuan Yin Yongping Yang Yuanwen Duan 《Plant Diversity》 SCIE CAS CSCD 北大核心 2017年第4期202-207,共6页
Floral traits, including those invisible to humans but visible to pollinators, that increase pollination efficiency may be selected by pollinators in plant species with pollen limitation of seed production, but the im... Floral traits, including those invisible to humans but visible to pollinators, that increase pollination efficiency may be selected by pollinators in plant species with pollen limitation of seed production, but the importance of pollinators as selective agents on different floral traits needs to be further quantified experimentally. In the present study, we examined selective strength on flower diameter, flower height,UV bulls-eye size, sepal size and UV proportion via female fitness in Caltha scaposa, based on openpollinated and hand-pollinated flowers, through which pollinator-mediated selection was calculated for each of floral traits. Our results suggest that seed production of C. scaposa is pollen limited in natural conditions. There was directional selection(△β_(pollinator)=-0.12) for larger flowers in open-pollinated flowers, while no significant selection was found in flower height, UV bulls-eye size, sepal size or UV proportion. Statistically significant selection was found in UV bulls-eye size, sepal size and UV proportion in hand-pollinated flowers, but interactions with pollinators contributed only to flower diameter. We conclude that in C. scaposa, floral traits that are subjected to selection might be driven by multiple selective agents, and suggest the importance of investigating floral traits that are invisible to human but visible to pollinators in measuring pollinator-mediated selection via male fitness. 展开更多
关键词 Pollinator-mediated selection Floral display Ultraviolet bulls-eye Female fitness Pollen limitation
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Epidermal visualized health monitoring system based on stretchable and washable TPU hybrid conductive microtextiles 被引量:1
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作者 Jing-Bo Yuan Zhi-Hong Feng +2 位作者 Dong-Chan Li Yang Luo Yun-Lei Zhou 《Rare Metals》 SCIE EI CAS CSCD 2024年第7期3185-3193,共9页
Electronic textiles,an emerging class of electronic technology,offer exciting opportunities for seamless integration with the human body.Numerous applications have been developed based on electronic textiles.However,r... Electronic textiles,an emerging class of electronic technology,offer exciting opportunities for seamless integration with the human body.Numerous applications have been developed based on electronic textiles.However,researches on integrating multiple electronic textilebased devices are still few.In this study,we present a system integrated with an electrocardiogram monitoring sensor and an electroluminescence device based on stretchable and washable conductive micro textiles.The signal is acquired by an electrocardiograph amplifier and displayed by a dual-color electroluminescence device based on the processed results.The integrated electronic device has excellent moisture permeability and comfort for long-term wearing.The system reported in this study opens a new avenue for the application of electronic textiles in health monitoring,robotic prosthetics,and competitive sports. 展开更多
关键词 Conductive microtextile Textile composite Electronic textile Electroluminescent displays
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An analysis and evaluation system of pulsed GMAW process based on LabVIEW 被引量:1
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作者 高莹 李桓 +2 位作者 凌礼恭 许贞龙 王颖 《China Welding》 EI CAS 2011年第3期22-26,共5页
A process evaluation system for pulsed gas metal arc welding (GMAW-P) based on the Lab V1EW platform has been developed. This system is comprised of two modules, a simultaneous display module and a data analysis mod... A process evaluation system for pulsed gas metal arc welding (GMAW-P) based on the Lab V1EW platform has been developed. This system is comprised of two modules, a simultaneous display module and a data analysis module. Using these modules, the system can not only provide a comprehensive direct viewing display of the welding electric signal and high speed camera photo, but also can analyze the characteristic parameters of the welding process. The results show that the system works properly. 展开更多
关键词 PULSE WELDING simultaneous display statistical analysis
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Simplification of integral imaging system by using a lenticular lens array 被引量:1
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作者 张雷 焦小雪 《Optoelectronics Letters》 EI 2014年第6期420-422,共3页
Integral imaging is a 3D display technology without any additional equipment. A new system is proposed in this paper, which uses a lenticular lens array to replace the lens array in the conventional integral imaging s... Integral imaging is a 3D display technology without any additional equipment. A new system is proposed in this paper, which uses a lenticular lens array to replace the lens array in the conventional integral imaging system. The lenticular integral imaging system reduces the complexity and the price of integral imaging system. The positive characteristics of conventional integral imaging system, such as full parallax and quasi-continuous view points, are still kept on the proposed system in horizontal direction. Optical results show that the time for calculating the elemental images is reduced by 25% compared with the conventional one. The resolution of integrated image in vertical direction is 4 times higher than that of conventional system. This proposed system opens a new way on application of integral imaging. 展开更多
关键词 Display devices Geometrical optics
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