This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation system.The calluses induced from buds of A.albus were disinfec...This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation system.The calluses induced from buds of A.albus were disinfected with benzalkonium bromide,mercury bichloride,sodium hypochlorite,and ethanol,respectively,for different time periods.The growth of calluses was compared between different treatments and observed in detail by dissection.The results showed that the disinfection with 0.1% mercury bichloride for 5 min and 75% ethanol for 5 s had mild effect on the callus,with the lowest browning rates of 16.32% and 15.46% and the lowest vitrification rates of 3.24% and 9.72%,respectively,after 20 d.The disinfection with benzalkonium bromide and sodium hypochlorite had strong effect on the callus cells,with the lowest browning rate of 50.69% and 25.12% and the lowest vitrification rates of 28.47% and 28.43%,respectively.The cytological observation result showed that the calluses disinfected with ethanol and mercury bichloride showcased compact structures,while those disinfected with sodium hypochlorite and benzalkonium bromide exhibited loose structures.To sum up,it is recommended that 75% ethanol or 0.1% mercury bichloride can be used for disinfection in the tissue culture and rapid propagation of A.albus,while the use of benzalkonium bromide and sodium hypochlorite should be avoided as much as possible.展开更多
As further promotion of the rural drinking water safety project in whole country, small villages in vast countryside have been or will be equipped with safety drinking water project. We analyzed necessity of the rural...As further promotion of the rural drinking water safety project in whole country, small villages in vast countryside have been or will be equipped with safety drinking water project. We analyzed necessity of the rural drinking water disinfection, discussed disinfection method suitable for rural drinking water characteristics, and put forward disinfection schemes for different water supply sources.展开更多
In recent years, China has given high attention and support to the animal husbandry industry. Among them, in the pig husbandry industry, the government has stepped up the management of the prevention and control of sw...In recent years, China has given high attention and support to the animal husbandry industry. Among them, in the pig husbandry industry, the government has stepped up the management of the prevention and control of swine epidemic disease. The first is to ensure the safety of pork and ancillary products flowing into the market, and the second is to ensure and improve the economic benefits of pig farmers. Based on this, pig farmers should earnestly do a good job in the prevention and control of swine epidemic disease, especially in the disinfection link in the daily feeding process of pigs, and should formulate reasonable and scientific disinfection strategies in combination with the specific conditions of actual breeding, and continuously upgrade and improve the prevention and disinfection methods of swine epidemic disease, so as to create a healthy environment for pig growth and achieve the purpose of economic income generation. The following article explores the importance of disinfection in the prevention and treatment of swine epidemic disease, and summarizes some commonly used and effective disinfection methods, which are only for the reference of farmers.展开更多
文摘This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation system.The calluses induced from buds of A.albus were disinfected with benzalkonium bromide,mercury bichloride,sodium hypochlorite,and ethanol,respectively,for different time periods.The growth of calluses was compared between different treatments and observed in detail by dissection.The results showed that the disinfection with 0.1% mercury bichloride for 5 min and 75% ethanol for 5 s had mild effect on the callus,with the lowest browning rates of 16.32% and 15.46% and the lowest vitrification rates of 3.24% and 9.72%,respectively,after 20 d.The disinfection with benzalkonium bromide and sodium hypochlorite had strong effect on the callus cells,with the lowest browning rate of 50.69% and 25.12% and the lowest vitrification rates of 28.47% and 28.43%,respectively.The cytological observation result showed that the calluses disinfected with ethanol and mercury bichloride showcased compact structures,while those disinfected with sodium hypochlorite and benzalkonium bromide exhibited loose structures.To sum up,it is recommended that 75% ethanol or 0.1% mercury bichloride can be used for disinfection in the tissue culture and rapid propagation of A.albus,while the use of benzalkonium bromide and sodium hypochlorite should be avoided as much as possible.
基金Supported by Science and Technology Plan Project in Nanning City,China(20131064)
文摘As further promotion of the rural drinking water safety project in whole country, small villages in vast countryside have been or will be equipped with safety drinking water project. We analyzed necessity of the rural drinking water disinfection, discussed disinfection method suitable for rural drinking water characteristics, and put forward disinfection schemes for different water supply sources.
文摘In recent years, China has given high attention and support to the animal husbandry industry. Among them, in the pig husbandry industry, the government has stepped up the management of the prevention and control of swine epidemic disease. The first is to ensure the safety of pork and ancillary products flowing into the market, and the second is to ensure and improve the economic benefits of pig farmers. Based on this, pig farmers should earnestly do a good job in the prevention and control of swine epidemic disease, especially in the disinfection link in the daily feeding process of pigs, and should formulate reasonable and scientific disinfection strategies in combination with the specific conditions of actual breeding, and continuously upgrade and improve the prevention and disinfection methods of swine epidemic disease, so as to create a healthy environment for pig growth and achieve the purpose of economic income generation. The following article explores the importance of disinfection in the prevention and treatment of swine epidemic disease, and summarizes some commonly used and effective disinfection methods, which are only for the reference of farmers.
