In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the i...In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.展开更多
[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in...[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in the production, Yangdao 6 （an indica） and Yangjing 4038 （a japonica）, were field grown using a direct-seeding method, and four treatments, wheat straw incorporation into soil and traditional flooding （SlF）, non-flooding and wheat straw mulching （NSM）, non-flooding and wheat straw incorporation into soil （NSl） and traditional flooding （no straw returned, Control, TF）, were imposed after sowing to maturity. Effects of direct-seeding with non-flooding and wheat residue returning patterns on CH4, N20 and CO2 emissions were investigated by using the method of static chamber-gas chromatographic tech- niques. [Result] Grain yield showed no significant difference between non-flooding and flooding treatments, but was significantly higher under the SlF than under any other treatments. The emission flux of CH4 and CO2 under TF and SlF exhibited a single peak curve, while changed little under the NSl and NSM The emission flux of N2Oshowed multiple perk curves for all the treatments. Compared with TF, SlF significantly increased mean emission flux of CH4 or N2O, decreased emission of N20, while NSl and NSM significantly decreased the mean emission flux of OH4, and increased emission flux of N2O and CO2. SIF also increased Green Warm Potential （GWP） of CH4, N2O and CO2 and the GWP per unit grain yield by 47.3%- 53.7% and 32.2%-39.4%, respectively. Both NSl and NSM decreased GWP by 24.2%-29.6% and 30.1%-35.5%, and the GWP per unit grain yield was decreased by 21.7-27.2% and 25.6%-31.1%, respectively. [Conelusion] both NSl and NSM could significantly reduce greenhouse effect of CH4, N2O and CO2 meanwhile maintain a high grain yield.展开更多
Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation.The phytohormone gibberellin(GA)has positive effects on mesocotyl elongation in rice.However,the ...Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation.The phytohormone gibberellin(GA)has positive effects on mesocotyl elongation in rice.However,the physiological and molecular basis underlying the regulation of mesocotyl elongation mediated by GA priming under deep-sowing conditions remains largely unclear.In the present study,we performed a physiological and comprehensive transcriptomic analysis of the function of GA priming in mesocotyl elongation and seedling emergence using a direct-seeding japonica rice cultivar ZH10 at a5-cm sowing depth.Physiological experiments indicated that GA priming significantly improved rice seedling emergence by increasing the activity of starch-metabolizing enzymes and compatible solute content to supply the energy essential for subsequent development.Transcriptomic analysis revealed 7074 differentially expressed genes(false discovery rate of<0.05,|log2(fold change)|of≥1)after GA priming.Furthermore,gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses revealed that genes associated with transcriptional regulation,plant hormone biosynthesis or signaling,and starch and sucrose metabolism were critical for GA-mediated promotion of rice mesocotyl elongation.Further analyses showed that the expression of the transcription factor(TF)genes(v-myb avian myeloblastosis viral oncogene homolog(MYB)alternative splicing 1(MYBAS1),phytochrome-interacting factors 1(PIF1),Oryza sativa teosinte branched 1/cycloidea/proliferating cell factor 5(Os TCP5),slender 1(SLN1),and mini zinc finger 1(MIF1)),plant hormone biosynthesis or signaling genes(brassinazole-resistant 1(BZR1),ent-kaurenoic acid oxidase-like(KAO),GRETCHEN HAGEN 3.2(GH3.2),and small auxin up RNA 36(SAUR36)),and starch and sucrose metabolism genes(α-amylases(AMY2 A and AMY1.4))was highly correlated with the mesocotyl elongation and deep-sowing tolerance response.These results enhance our understanding of how nutrient metabolism-related substances and genes regulate rice mesocotyl elongation.This may facilitate future studies on related genes and the development of novel rice varieties tolerant to deep sowing.展开更多
The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing y...The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing yield.This study aimed to examine the effects of paclobutrazol seed soaking(PSS)on non-structural carbohydrate accumulation and grain enrichment in DSR,potentially providing a theoretical foundation for achieving high-yield DSR cultivation.The experiment utilized two rice varieties,Jiyujing(JYJ)and Jijing305(JJ305),with seeds soaked in paclobutrazol concentrations of 0 mg L^(−1) and 100 mg L^(−1).PSS demonstrated increased chlorophyll content,net photosynthetic rate,and leaf area,as well as an extended photosynthetic function period during the filling stage.It also elevated soluble sugar and starch contents in the flag leaf(during the filling stage)and stem sheath(after heading),decreased starch content in the top panicle while increasing it in the middle and lower panicle during the filling stage,and enhanced spikelet per unit area and seed setting rate,thereby improving DSR yield.In conclusion,PSS enhanced the photosynthetic capacity of DSR during the filling stage,coordinated the filling process of superior and inferior grains,maintained source-sink balance,and facilitated stable and orderly filling,ultimately resulting in improved yield.展开更多
The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses ...The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses substantial challenges.Issues such as water scarcity,energy crises,escalating greenhouse gas emissions,and diminishing farm profitability threaten longterm agricultural sustainability.In response,we applied a holistic food–carbon–nitrogen–water–energy–profit (FCNWEP)nexus framework to comprehensively assess the sustainability of distinct crop management strategies across three subsites in Central China.Field experiments were conducted in Hubei and Hunan provinces from 2017 to 2021 using a widely adopted elite super hybrid rice cultivar (Y-liangyou 900).Four crop management treatments were implemented:a control(CK,0 kg N ha^(-1)),conventional crop management (CCM,210–250 kg N ha^(-1),7:3 basal:mid-tiller fertilizer ratio),and two integrated crop management (ICM) treatments (ICM1,180–210 kg N ha^(-1),5:2:3 basal:mid-tiller:panicle initiation fertilizer ratio;ICM2,240–270 kg N ha^(-1),5:2:2:1 basal:mid-tiller:panicle initiation:flowering fertilizer ratio).Variables assessed included grain yield,carbon footprint,nitrogen footprint,water footprint,energy footprint,nitrogen use efficiency,and economic benefits.Our results showed significant yield variations,with ICM2 consistently outperforming CCM and ICM1across all three sites.In Jingzhou,Suizhou,and Changsha,ICM2’s grain yield was 30.2,24.7,and 13.3%higher than CCM,respectively.Net profits under ICM2 exceeded those of CCM and ICM1 by 31.8 and 115.2%in Jingzhou,32.2 and 109.9%in Suizhou,and 15.4 and 34.0%in Changsha,respectively.Integrated crop management,particularly ICM2,demonstrated improved nitrogen and energy use efficiency,leading to reduced carbon,nitrogen,water,and energy footprints.Overall,composite sustainability scores derived from the FCNWEP framework indicated that both ICM2 and ICM1 exhibited higher sustainability levels compared to CCM.This study provides valuable insights into practical management methodologies and offers recommendations for enhancing agricultural sustainability.展开更多
To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil ...To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil and plant samples were collected from seven newly reconstructed fields in Japanese Andosols in Tochigi,Japan.Samples were obtained from both the former low-and high-elevation sides within each field plot.During harvest season,nine rice plants were randomly selected from each plot(0.675 m^(2),comprising 3 rows and 3 hills per row),collected from a 3-m stretch along both the east(former low side)and west(former high side)ridges.Soil cores were collected from identical plots at two depths(0–15 and 15–30 cm)and combined into one composite sample per layer.Rice plant samples were air-dried for two weeks until reaching constant moisture content,after which stems and ears were separated and weighed to determine biomass,yield,yield components,and nitrogen uptake.This indicated that land reconstruction significantly affected rice yield and its components between the two sides of all field plots.SOC,TN,and their decomposition following land reconstruction showed notable changes,especially in the 15–30 cm subsurface soil layer.Additionally,grain weight demonstrated significant correlation with SOC,TN,and carbon decomposition in both the 0–15 and 15–30 cm layers,indicating that soil fertility to a depth of 30 cm was crucial for rice productivity after land reconstruction.展开更多
Spikelet filling characteristics in early-season rice in southern China may be distinctive due to its exposure to high temperatures during the ripening period.However,limited information is currently available on thes...Spikelet filling characteristics in early-season rice in southern China may be distinctive due to its exposure to high temperatures during the ripening period.However,limited information is currently available on these characteristics.This study aimed to characterize spikelet filling in early-season rice and identify the key factors contributing to its improvement.Field experiments were conducted over two years(2021 and 2022)to mainly investigate the proportions of fully-filled,partially-filled,and empty spikelets,along with the biomass-fertilized spikelet ratio and harvest index,in 11 early-season rice varieties.The results revealed significant varietal variation in spikelet filling,with the proportion of fully-filled spikelets ranging from 60.6%to 81.1%in 2021 and from 66.3%to 79.2%in 2022.Among the 11 varieties,Liangyou 42,Lingliangyou 942,and Liangyou 287 exhibited relatively superior performance in spikelet filling.Linear regression revealed that,although a significant negative relationship existed between the proportion of fully-filled spikelets and both partially-filled and empty spikelets,the relationship with partially-filled spikelets was stronger.Additionally,the proportion of fully-filled spikelets showed a significant positive relationship with the harvest index but not with the biomass-fertilized spikelet ratio.These findings indicate that increasing the harvest index and reducing the occurrence of partially-filled grains are essential strategies for improving spikelet filling in early-season rice.展开更多
Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population in...Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.展开更多
Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding ...Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding stress,exploring both the methodology and physiological mechanisms involved.The optimal seed soaking concentration was determined through a gradient experiment,followed by a multi-cultivar validation test.The physiological mechanism of wood vinegar soaking on seedling emergence was analyzed by measuring the electrical conductivity of the flooding water,the changes in starch and soluble sugar contents in the grains and sprouts,and the dynamics ofα-amylase activity and antioxidant-related enzyme activities in the sprouts.The results showed that soaking rice seeds in a wood vinegar solution at a low concentration significantly enhanced the emergence of rice seedlings under flooding conditions,with a 100-fold dilution having the most pronounced effect,increasing seedling rates by 50.6%-60.0%.Further analysis indicated that wood vinegar treatment enhanced seedling establishment by inducing a significant increase inα-amylase activity,leading to a 74.9%-213.6%increase in soluble sugar content in the sprouts during 2-8 d after flooding stress compared with the control.Additionally,the treatment increased superoxide dismutase and peroxidase activities in the sprouts,mitigating lipid peroxidation of the cell membranes,and notably lower water electrical conductivity was observed in wood vinegar-treated seeds compared with the control.In conclusion,soaking rice seeds in a 100-fold diluted wood vinegar solution improves rice seedling rates under flooding stress by mitigating oxidative damage and maintaining energy supply.This approach is valuable for developing cost-effective seed treatment technologies and offering novel strategies to improve seedling rates and uniformity of direct-seeded rice under flooding conditions.展开更多
Rice bran is the outer layer of the rice grain and a by-product of milling rice kernels,possessing high nutritional and therapeutic value.It is abundant in dietary fibers,vitamins,minerals,polyphenols,and various anti...Rice bran is the outer layer of the rice grain and a by-product of milling rice kernels,possessing high nutritional and therapeutic value.It is abundant in dietary fibers,vitamins,minerals,polyphenols,and various antioxidant molecules.The health-promoting effects of rice bran and its biomolecules have been documented in several studies.In this review,we evaluated the different nutritive and health-promoting effects of rice bran,particularly its impact on gut health and other chronic conditions associated with gut health.The biomolecules present in rice bran and their potential therapeutic effects were also summarized.This paper recapitulated the potential therapeutic and preventive efficacy of rice bran against various ailments,along with their mechanisms of action.Rice bran is an important source of nutritive substances.In addition to their nutritive value,rice bran is rich in diverse biomolecules such as anthocyanins,flavonoids,phenolics,γ-oryzanol,phytosterols and derivatives,saturated fatty acids,monounsaturated fatty acids,and polyunsaturated fatty acids.The dietary fibers in rice bran play a key role in modulating gut microbiota,reducing inflammation,and maintaining gut health.Rice bran and its components have been found to exhibit therapeutic benefits against inflammation,diabetes,cancer,liver disorders,cardiac issues,and neurological disorders through various mechanisms.Different clinical investigations have also confirmed the potential beneficial effects of rice bran and rice bran oil in various metabolic and cardiac disease conditions.Modulating gut microbiota is an important mechanism of the beneficial effects exerted by rice bran.This comprehensive review underscores the nutritional and therapeutic value of rice bran,emphasizing its potential for wider adoption to address nutrient deficiencies and improve human health.展开更多
In order to realize the comprehensive utilization of industrial solid waste rice husk ash and heavy metal cadmium contaminated soil,rice husk ash-based geopolymer prepared by alkaline activator was used to modify cadm...In order to realize the comprehensive utilization of industrial solid waste rice husk ash and heavy metal cadmium contaminated soil,rice husk ash-based geopolymer prepared by alkaline activator was used to modify cadmium contaminated soil.The main physical and chemical properties of rice husk ash were clarified by SEM,XRF and X-ray diffraction.The unconfined compressive strength test and toxicity leaching test were carried out on the modified soil.Combined with FTIR and TG micro-level,the solidification mechanism of rice husk ash-based geopolymer solidified cadmium contaminated soil was discussed.The results show that the strength of geopolymer modified soil is significantly higher than that of plain soil,and the unconfined compressive strength at 7 d age is 4.2 times that of plain soil.The strength of modified soil with different dosage of geopolymer at 28 d age is about 36% to 40% higher than that of modified soil at 7 d age.Geopolymer has a significant effect on the leaching of heavy metals in contaminated soil.When the cadmium content is 100 mg/kg,it meets the standard limit.In the process of complex depolymerization-condensation reaction,on the one hand,geopolymers are cemented and agglomerated to form a complex spatial structure,which affects the macro and micro characteristics of soil.On the other hand,it has significant adsorption,precipitation and replacement effects on heavy metal ions in soil,showing good strength and low heavy metal leaching toxicity.展开更多
As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy...As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture.展开更多
The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza s...The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.展开更多
A green pregelatinized glutinous rice flour biological admixture was developed in this paper.The cement hydration process,hydration products,pore structure,and strength of mortar with different quantities of glutinous...A green pregelatinized glutinous rice flour biological admixture was developed in this paper.The cement hydration process,hydration products,pore structure,and strength of mortar with different quantities of glutinous rice flour(GRF),and the macroscopic changes in concrete cracking resistance testing were investigated.Simultaneously,a fast cracking resistance evaluation method based on graphic recognition was proposed.The results indicated that pregelatinized glutinous rice flour(T-GRF)delayed the dissolution rate of anhydrous cement during the induction period,shifting the main exothermic peak of hydration backward.The compressive strength developed slowly in 7-28 d age and returned to normal in 28-56 d.The compressive strength of T-GRF-0.6% modified mortar at 56 d age is less than 10% different from that of control group.The 3.0%T-GRF decreased the total porosity by 3%,and the average pore size decreased from 31.2 to 21.3 nm measured by MIP,indicating that T-GRF could inhibit harmful pores and densify concrete.The crack resistance coefficient of T-GRF modified concrete was obtained by image recognition method,and the GRF could decrease the length,width,and damaged area of cracks in the early age of concrete.展开更多
[Objectives]This study was conducted to achieve rapid and accurate detection of protein content in rice with a particle size of 1.0 mm.[Methods]A multi-model fusion strategy was proposed on the basis of Stacking ensem...[Objectives]This study was conducted to achieve rapid and accurate detection of protein content in rice with a particle size of 1.0 mm.[Methods]A multi-model fusion strategy was proposed on the basis of Stacking ensemble learning.A base learner pool was constructed,containing Partial Least Squares(PLS),Support Vector Machine(SVM),Deep Extreme Learning Machine(DELM),Random Forest(RF),Gradient Boosting Decision Tree(GBDT),and Multilayer Perceptron(MLP).PLS,DELM,and Linear Regression(LR)were used as meta-learner candidates.Employing integer coding technology,systematic dynamic combinations of base learners and meta-learners were generated,resulting in a total of 40 non-repetitive fusion models.The optimal combination was selected through a comprehensive evaluation based on multiple assessment indicators.[Results]The combination"PLS-DELM-MLP-LR"(code 1367)achieved coefficients of determination of 0.9732 and 0.9780 on the validation set and independent test set,respectively,with relative root mean square errors of 2.35%and 2.36%,and residual predictive deviations of 6.1075 and 6.7479,respectively.[Conclusions]The Stacking fusion model significantly enhances the predictive accuracy and robustness of spectral quantitative analysis,providing an efficient and feasible solution for modeling complex agricultural product spectral data.展开更多
As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a ...As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a crucial role in rice growth and development,stress response,and hormone signal transduction.This review discusses the role of WRKY53 in stress response,focusing on its functions in cold tolerance,salt tolerance,disease resistance,and pest defense,and explores its role in regulating rice leaf senescence and seed germination.This article also proposes future research directions,including functional genomics studies,protein interaction network analyses,hormone signal transduction pathways,genetic improvement strategies,applications of gene editing technologies,molecular basis of stress responses,cross-species functional conservation,and bioinformatics and comparative genomics research.This review highlights the importance of WRKY53 in rice biology and provides new perspectives and strategies for future research and genetic improvement of rice.展开更多
Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural...Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural management and achieve crop yield potential.Compared with traditional parameters,canopy occupation volume(COV)offers an integrative parameter on canopy architecture related to canopy photosynthetic rates.In this study,we developed a high-throughput method to derive COV for different rice varieties.We first used multi-perspective two-dimensional imaging to reconstruct three-dimensional point clouds of rice plants and developed a suite of pipelines to calculate plant height,leaf number,tiller number,and biomass,with R^(2) values of 91.8%,95.9%,82.3%,and 94.3%,respectively.We further employed point cloud data to reconstruct the surfaces of rice plants and construct a virtual canopy model of the rice population.Light distribution was simulated using a ray-tracing algorithm and canopy photosynthetic rates were simulated via photosynthetic rate-incident light intensity curve fitting.Furthermore,we systematically explored the relationships between canopy phenotypes and photosynthetic rates,and found that COV was the most effective predictor of canopy photosynthesis,achieving an R^(2) value of 92.1%.Adjustment in atmospheric transmittance showed that COV strongly correlated with canopy photosynthesis under different light conditions,with higher accuracy observed under diffuse light.Variations in planting density confirmed that this correlation remained strong at the community level.In summary,this study demonstrates that COV is closely linked to simulated canopy photosynthesis and the developed pipeline can support future agronomic and breeding research.展开更多
Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to h...Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to humans,and the International Agency for Research on Cancer has classified it as a Group I carcinogen.Cadmium undergoes minimal metabolism in the human body;consequently,prolonged Cd^(2+)exposure can cause severe damage to multiple organs including the liver,kidneys,lungs,bones,and immune system(Shao et al.2024).Rice,one of the three global staple crops,and Cd exposure in humans primarily occurs the consumption of contaminated rice grains.The contribution of rice to the total dietary Cd intake is over 50% for non-smoking Asian populations(Chen et al.2018;Shi et al.2020).展开更多
基金supported by the General Project of Humanities and Social Sciences Research of the Ministry of Education of China(24YJA790085)the Science and Technology Innovation Program of Beijing Institute of Technology,China(2024CX01020)。
文摘In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.
基金Supported by National Natural Science Foundation of China(31371562,31301276)Special Fund for Fundamental Scientific Research Business of Central Public Research Institutes(Agriculture)(201103003+2 种基金201203079)Key Projects in the National Science&Technology Pillar Program during the 12thFive-year Plan Period(2012BAD04B08)Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(14)5021)~~
文摘[Objective] This study aimed to investigate the effect of direct-seeding with non-flooding and wheat residue returning patterns on greenhouse gas emission from rice paddy. [Method] Two rice cultivars currently used in the production, Yangdao 6 （an indica） and Yangjing 4038 （a japonica）, were field grown using a direct-seeding method, and four treatments, wheat straw incorporation into soil and traditional flooding （SlF）, non-flooding and wheat straw mulching （NSM）, non-flooding and wheat straw incorporation into soil （NSl） and traditional flooding （no straw returned, Control, TF）, were imposed after sowing to maturity. Effects of direct-seeding with non-flooding and wheat residue returning patterns on CH4, N20 and CO2 emissions were investigated by using the method of static chamber-gas chromatographic tech- niques. [Result] Grain yield showed no significant difference between non-flooding and flooding treatments, but was significantly higher under the SlF than under any other treatments. The emission flux of CH4 and CO2 under TF and SlF exhibited a single peak curve, while changed little under the NSl and NSM The emission flux of N2Oshowed multiple perk curves for all the treatments. Compared with TF, SlF significantly increased mean emission flux of CH4 or N2O, decreased emission of N20, while NSl and NSM significantly decreased the mean emission flux of OH4, and increased emission flux of N2O and CO2. SIF also increased Green Warm Potential （GWP） of CH4, N2O and CO2 and the GWP per unit grain yield by 47.3%- 53.7% and 32.2%-39.4%, respectively. Both NSl and NSM decreased GWP by 24.2%-29.6% and 30.1%-35.5%, and the GWP per unit grain yield was decreased by 21.7-27.2% and 25.6%-31.1%, respectively. [Conelusion] both NSl and NSM could significantly reduce greenhouse effect of CH4, N2O and CO2 meanwhile maintain a high grain yield.
基金supported by the National Key Research and Development Program of China(No.2016YFD0100101-19)the Rice Industry Technology System of Henan Province(No.S2012-04)the Independent Innovation Fund Program of Henan Academy of Agricultural Sciences(No.2020ZC07)。
文摘Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation.The phytohormone gibberellin(GA)has positive effects on mesocotyl elongation in rice.However,the physiological and molecular basis underlying the regulation of mesocotyl elongation mediated by GA priming under deep-sowing conditions remains largely unclear.In the present study,we performed a physiological and comprehensive transcriptomic analysis of the function of GA priming in mesocotyl elongation and seedling emergence using a direct-seeding japonica rice cultivar ZH10 at a5-cm sowing depth.Physiological experiments indicated that GA priming significantly improved rice seedling emergence by increasing the activity of starch-metabolizing enzymes and compatible solute content to supply the energy essential for subsequent development.Transcriptomic analysis revealed 7074 differentially expressed genes(false discovery rate of<0.05,|log2(fold change)|of≥1)after GA priming.Furthermore,gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses revealed that genes associated with transcriptional regulation,plant hormone biosynthesis or signaling,and starch and sucrose metabolism were critical for GA-mediated promotion of rice mesocotyl elongation.Further analyses showed that the expression of the transcription factor(TF)genes(v-myb avian myeloblastosis viral oncogene homolog(MYB)alternative splicing 1(MYBAS1),phytochrome-interacting factors 1(PIF1),Oryza sativa teosinte branched 1/cycloidea/proliferating cell factor 5(Os TCP5),slender 1(SLN1),and mini zinc finger 1(MIF1)),plant hormone biosynthesis or signaling genes(brassinazole-resistant 1(BZR1),ent-kaurenoic acid oxidase-like(KAO),GRETCHEN HAGEN 3.2(GH3.2),and small auxin up RNA 36(SAUR36)),and starch and sucrose metabolism genes(α-amylases(AMY2 A and AMY1.4))was highly correlated with the mesocotyl elongation and deep-sowing tolerance response.These results enhance our understanding of how nutrient metabolism-related substances and genes regulate rice mesocotyl elongation.This may facilitate future studies on related genes and the development of novel rice varieties tolerant to deep sowing.
基金National Key Research and Development Project of China(2022yfd1500501)Jilin Province Science and Technology Department Outstanding Young Talent Fund Project(20230508001RC).
文摘The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing yield.This study aimed to examine the effects of paclobutrazol seed soaking(PSS)on non-structural carbohydrate accumulation and grain enrichment in DSR,potentially providing a theoretical foundation for achieving high-yield DSR cultivation.The experiment utilized two rice varieties,Jiyujing(JYJ)and Jijing305(JJ305),with seeds soaked in paclobutrazol concentrations of 0 mg L^(−1) and 100 mg L^(−1).PSS demonstrated increased chlorophyll content,net photosynthetic rate,and leaf area,as well as an extended photosynthetic function period during the filling stage.It also elevated soluble sugar and starch contents in the flag leaf(during the filling stage)and stem sheath(after heading),decreased starch content in the top panicle while increasing it in the middle and lower panicle during the filling stage,and enhanced spikelet per unit area and seed setting rate,thereby improving DSR yield.In conclusion,PSS enhanced the photosynthetic capacity of DSR during the filling stage,coordinated the filling process of superior and inferior grains,maintained source-sink balance,and facilitated stable and orderly filling,ultimately resulting in improved yield.
基金funded by the National Natural Science Foundation of China (32172108 and 32301940)the Young Elite Scientists Sponsorship Program by the China Association for Science and Technology (2023QNRC001)+2 种基金the China Postdoctoral Science Foundation (2022M710489)the Chinese Scholarship Council (202310930003)the National Key Research and Development Program of China (2022YFD2301004)。
文摘The breakthrough in super hybrid rice yield has significantly contributed to China’s and global food security.However,the inherent conflict between high productivity and environmentally sustainable agriculture poses substantial challenges.Issues such as water scarcity,energy crises,escalating greenhouse gas emissions,and diminishing farm profitability threaten longterm agricultural sustainability.In response,we applied a holistic food–carbon–nitrogen–water–energy–profit (FCNWEP)nexus framework to comprehensively assess the sustainability of distinct crop management strategies across three subsites in Central China.Field experiments were conducted in Hubei and Hunan provinces from 2017 to 2021 using a widely adopted elite super hybrid rice cultivar (Y-liangyou 900).Four crop management treatments were implemented:a control(CK,0 kg N ha^(-1)),conventional crop management (CCM,210–250 kg N ha^(-1),7:3 basal:mid-tiller fertilizer ratio),and two integrated crop management (ICM) treatments (ICM1,180–210 kg N ha^(-1),5:2:3 basal:mid-tiller:panicle initiation fertilizer ratio;ICM2,240–270 kg N ha^(-1),5:2:2:1 basal:mid-tiller:panicle initiation:flowering fertilizer ratio).Variables assessed included grain yield,carbon footprint,nitrogen footprint,water footprint,energy footprint,nitrogen use efficiency,and economic benefits.Our results showed significant yield variations,with ICM2 consistently outperforming CCM and ICM1across all three sites.In Jingzhou,Suizhou,and Changsha,ICM2’s grain yield was 30.2,24.7,and 13.3%higher than CCM,respectively.Net profits under ICM2 exceeded those of CCM and ICM1 by 31.8 and 115.2%in Jingzhou,32.2 and 109.9%in Suizhou,and 15.4 and 34.0%in Changsha,respectively.Integrated crop management,particularly ICM2,demonstrated improved nitrogen and energy use efficiency,leading to reduced carbon,nitrogen,water,and energy footprints.Overall,composite sustainability scores derived from the FCNWEP framework indicated that both ICM2 and ICM1 exhibited higher sustainability levels compared to CCM.This study provides valuable insights into practical management methodologies and offers recommendations for enhancing agricultural sustainability.
基金support of the Japanese Government(Monbukagakusho)Scholarship for his studies in Japansupported by the Yamagata University YU-COE(S)program and by the Advanced Agri-food System Research Center of Yamagata University,Japan+2 种基金financially supported by a Japan Society for the Promotion of Science(JSPS)Grant-in-Aid for Scientific Research(26310304)Yamagata University YU-COE(S)programby the Advanced Agri-food System Research Center of Yamagata University,Japan。
文摘To examine the impact of anthropogenic land reconstruction,particularly the consolidation of small terraces into larger fields,on soil organic carbon(SOC),total nitrogen(TN)dynamics,rice yield,and its components,soil and plant samples were collected from seven newly reconstructed fields in Japanese Andosols in Tochigi,Japan.Samples were obtained from both the former low-and high-elevation sides within each field plot.During harvest season,nine rice plants were randomly selected from each plot(0.675 m^(2),comprising 3 rows and 3 hills per row),collected from a 3-m stretch along both the east(former low side)and west(former high side)ridges.Soil cores were collected from identical plots at two depths(0–15 and 15–30 cm)and combined into one composite sample per layer.Rice plant samples were air-dried for two weeks until reaching constant moisture content,after which stems and ears were separated and weighed to determine biomass,yield,yield components,and nitrogen uptake.This indicated that land reconstruction significantly affected rice yield and its components between the two sides of all field plots.SOC,TN,and their decomposition following land reconstruction showed notable changes,especially in the 15–30 cm subsurface soil layer.Additionally,grain weight demonstrated significant correlation with SOC,TN,and carbon decomposition in both the 0–15 and 15–30 cm layers,indicating that soil fertility to a depth of 30 cm was crucial for rice productivity after land reconstruction.
基金funded by the Earmarked Fund for China Agriculture Research System,grant number CARS-01-33.
文摘Spikelet filling characteristics in early-season rice in southern China may be distinctive due to its exposure to high temperatures during the ripening period.However,limited information is currently available on these characteristics.This study aimed to characterize spikelet filling in early-season rice and identify the key factors contributing to its improvement.Field experiments were conducted over two years(2021 and 2022)to mainly investigate the proportions of fully-filled,partially-filled,and empty spikelets,along with the biomass-fertilized spikelet ratio and harvest index,in 11 early-season rice varieties.The results revealed significant varietal variation in spikelet filling,with the proportion of fully-filled spikelets ranging from 60.6%to 81.1%in 2021 and from 66.3%to 79.2%in 2022.Among the 11 varieties,Liangyou 42,Lingliangyou 942,and Liangyou 287 exhibited relatively superior performance in spikelet filling.Linear regression revealed that,although a significant negative relationship existed between the proportion of fully-filled spikelets and both partially-filled and empty spikelets,the relationship with partially-filled spikelets was stronger.Additionally,the proportion of fully-filled spikelets showed a significant positive relationship with the harvest index but not with the biomass-fertilized spikelet ratio.These findings indicate that increasing the harvest index and reducing the occurrence of partially-filled grains are essential strategies for improving spikelet filling in early-season rice.
基金funded by the National Key R&D Project‘Innovation and Integration of Key Technologies for Integration of Agricultural Machinery and Agronomy in Weak Links of Hybrid Mid-season Rice in Hilly Areas of Southwest China’(2023YFD2301901).
文摘Chilo suppressalis(Walker)is one of the most important rice pests worldwide,posing a significant challenge to effective control.To develop a precision-timed,eco-friendly management strategy,overwintering population investigation and dynamic monitoring of C.suppressalis populations were conducted in the Meishan region of Sichuan,China,from 2023 to 2024.The optimal timing for insecticide application was estimated,followed by field trials evaluating the efficacy of different insecticides.Results demonstrated that the peak emergence of first-generation adults typically occurred in early July(under the environmental conditions of the Meishan region),with the ambient humidity below 75%and temperature around 29◦C.Pesticide efficacy trials show that insecticide combinations exhibited superior control.Notably,a combined treatment of emamectin benzoate⋅methoxyfenozide+chlorantraniliprole achieved the highest control efficacy(90.05%)and a corresponding yield of 12,491.55 kg/ha.All tested treatments were determined to be safe for rice growth.Furthermore,this optimized strategy resulted in notable economic benefits,including a 50%reduction in pesticide usage and cost savings of 4796.15 CNY compared to conventional practices.This study provides valuable insights into sustainable rice production and pest management and,for the first time,proposes a precision application time window based on intelligent monitoring.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD2301300)the National Rice Industry Technology System,China(Grant No.CARS-01).
文摘Flooding stress is a major adverse condition during the emergence period of direct-seeded rice.This study investigated the use of wood vinegar as a seed soaking treatment to enhance rice seedling rates under flooding stress,exploring both the methodology and physiological mechanisms involved.The optimal seed soaking concentration was determined through a gradient experiment,followed by a multi-cultivar validation test.The physiological mechanism of wood vinegar soaking on seedling emergence was analyzed by measuring the electrical conductivity of the flooding water,the changes in starch and soluble sugar contents in the grains and sprouts,and the dynamics ofα-amylase activity and antioxidant-related enzyme activities in the sprouts.The results showed that soaking rice seeds in a wood vinegar solution at a low concentration significantly enhanced the emergence of rice seedlings under flooding conditions,with a 100-fold dilution having the most pronounced effect,increasing seedling rates by 50.6%-60.0%.Further analysis indicated that wood vinegar treatment enhanced seedling establishment by inducing a significant increase inα-amylase activity,leading to a 74.9%-213.6%increase in soluble sugar content in the sprouts during 2-8 d after flooding stress compared with the control.Additionally,the treatment increased superoxide dismutase and peroxidase activities in the sprouts,mitigating lipid peroxidation of the cell membranes,and notably lower water electrical conductivity was observed in wood vinegar-treated seeds compared with the control.In conclusion,soaking rice seeds in a 100-fold diluted wood vinegar solution improves rice seedling rates under flooding stress by mitigating oxidative damage and maintaining energy supply.This approach is valuable for developing cost-effective seed treatment technologies and offering novel strategies to improve seedling rates and uniformity of direct-seeded rice under flooding conditions.
文摘Rice bran is the outer layer of the rice grain and a by-product of milling rice kernels,possessing high nutritional and therapeutic value.It is abundant in dietary fibers,vitamins,minerals,polyphenols,and various antioxidant molecules.The health-promoting effects of rice bran and its biomolecules have been documented in several studies.In this review,we evaluated the different nutritive and health-promoting effects of rice bran,particularly its impact on gut health and other chronic conditions associated with gut health.The biomolecules present in rice bran and their potential therapeutic effects were also summarized.This paper recapitulated the potential therapeutic and preventive efficacy of rice bran against various ailments,along with their mechanisms of action.Rice bran is an important source of nutritive substances.In addition to their nutritive value,rice bran is rich in diverse biomolecules such as anthocyanins,flavonoids,phenolics,γ-oryzanol,phytosterols and derivatives,saturated fatty acids,monounsaturated fatty acids,and polyunsaturated fatty acids.The dietary fibers in rice bran play a key role in modulating gut microbiota,reducing inflammation,and maintaining gut health.Rice bran and its components have been found to exhibit therapeutic benefits against inflammation,diabetes,cancer,liver disorders,cardiac issues,and neurological disorders through various mechanisms.Different clinical investigations have also confirmed the potential beneficial effects of rice bran and rice bran oil in various metabolic and cardiac disease conditions.Modulating gut microbiota is an important mechanism of the beneficial effects exerted by rice bran.This comprehensive review underscores the nutritional and therapeutic value of rice bran,emphasizing its potential for wider adoption to address nutrient deficiencies and improve human health.
基金Funded by Central Guiding Local Science and Technology Development Special Fund Project(No.ZYYD2023B02)Innovation and Entrepreneurship Training Program for College Students in Xinjiang Uygur Autonomous Region(No.S202410994015)+2 种基金China University of Mining and Technology Coal Fine Exploration and Intelligent Development National Key Laboratory Xinjiang Engineering College Joint Fund(No.SKLCRSM-XJIE24KF001)Basic Research Funds for Autonomous Region Universities(No.XJEDU2024P082)National Natural Science Foundation of China(No.41662017)。
文摘In order to realize the comprehensive utilization of industrial solid waste rice husk ash and heavy metal cadmium contaminated soil,rice husk ash-based geopolymer prepared by alkaline activator was used to modify cadmium contaminated soil.The main physical and chemical properties of rice husk ash were clarified by SEM,XRF and X-ray diffraction.The unconfined compressive strength test and toxicity leaching test were carried out on the modified soil.Combined with FTIR and TG micro-level,the solidification mechanism of rice husk ash-based geopolymer solidified cadmium contaminated soil was discussed.The results show that the strength of geopolymer modified soil is significantly higher than that of plain soil,and the unconfined compressive strength at 7 d age is 4.2 times that of plain soil.The strength of modified soil with different dosage of geopolymer at 28 d age is about 36% to 40% higher than that of modified soil at 7 d age.Geopolymer has a significant effect on the leaching of heavy metals in contaminated soil.When the cadmium content is 100 mg/kg,it meets the standard limit.In the process of complex depolymerization-condensation reaction,on the one hand,geopolymers are cemented and agglomerated to form a complex spatial structure,which affects the macro and micro characteristics of soil.On the other hand,it has significant adsorption,precipitation and replacement effects on heavy metal ions in soil,showing good strength and low heavy metal leaching toxicity.
基金Supported by Natural Science Foundation General Project of Heilongjiang Province(C2018050).
文摘As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture.
基金supported by the National Key R&D Program,China(Grant No.2022YFD1201505)the Key Laboratory of Sichuan Province Open Project,China(Grant No.2023LYKF02)+1 种基金the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202306)the Sichuan Provincial Financial Independent Innovation Project,China(Grant No.2022ZZCX001).
文摘The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.
基金Funded by Hainan Provincial Natural Science Foundation(No.522QN279)State Key Laboratory of High Performance Civil Engineering Materials(No.2023CEM004)。
文摘A green pregelatinized glutinous rice flour biological admixture was developed in this paper.The cement hydration process,hydration products,pore structure,and strength of mortar with different quantities of glutinous rice flour(GRF),and the macroscopic changes in concrete cracking resistance testing were investigated.Simultaneously,a fast cracking resistance evaluation method based on graphic recognition was proposed.The results indicated that pregelatinized glutinous rice flour(T-GRF)delayed the dissolution rate of anhydrous cement during the induction period,shifting the main exothermic peak of hydration backward.The compressive strength developed slowly in 7-28 d age and returned to normal in 28-56 d.The compressive strength of T-GRF-0.6% modified mortar at 56 d age is less than 10% different from that of control group.The 3.0%T-GRF decreased the total porosity by 3%,and the average pore size decreased from 31.2 to 21.3 nm measured by MIP,indicating that T-GRF could inhibit harmful pores and densify concrete.The crack resistance coefficient of T-GRF modified concrete was obtained by image recognition method,and the GRF could decrease the length,width,and damaged area of cracks in the early age of concrete.
文摘[Objectives]This study was conducted to achieve rapid and accurate detection of protein content in rice with a particle size of 1.0 mm.[Methods]A multi-model fusion strategy was proposed on the basis of Stacking ensemble learning.A base learner pool was constructed,containing Partial Least Squares(PLS),Support Vector Machine(SVM),Deep Extreme Learning Machine(DELM),Random Forest(RF),Gradient Boosting Decision Tree(GBDT),and Multilayer Perceptron(MLP).PLS,DELM,and Linear Regression(LR)were used as meta-learner candidates.Employing integer coding technology,systematic dynamic combinations of base learners and meta-learners were generated,resulting in a total of 40 non-repetitive fusion models.The optimal combination was selected through a comprehensive evaluation based on multiple assessment indicators.[Results]The combination"PLS-DELM-MLP-LR"(code 1367)achieved coefficients of determination of 0.9732 and 0.9780 on the validation set and independent test set,respectively,with relative root mean square errors of 2.35%and 2.36%,and residual predictive deviations of 6.1075 and 6.7479,respectively.[Conclusions]The Stacking fusion model significantly enhances the predictive accuracy and robustness of spectral quantitative analysis,providing an efficient and feasible solution for modeling complex agricultural product spectral data.
基金supported by the Hubei Provincial Natural Science Foundation,China(Grant No.2024AFB917).
文摘As sessile organisms,plants must adapt various stresses.Accordingly,they have evolved several plant-specific growth and developmental processes.WRKY53 is a member of the WRKY transcription factor family,which plays a crucial role in rice growth and development,stress response,and hormone signal transduction.This review discusses the role of WRKY53 in stress response,focusing on its functions in cold tolerance,salt tolerance,disease resistance,and pest defense,and explores its role in regulating rice leaf senescence and seed germination.This article also proposes future research directions,including functional genomics studies,protein interaction network analyses,hormone signal transduction pathways,genetic improvement strategies,applications of gene editing technologies,molecular basis of stress responses,cross-species functional conservation,and bioinformatics and comparative genomics research.This review highlights the importance of WRKY53 in rice biology and provides new perspectives and strategies for future research and genetic improvement of rice.
基金supported by the National Natural Science Foundation of China(Grant Nos.32201654 and U22A20464)National Key Research and Development Program from the Ministry of Science and Technology of China(Grant No.2020YFA0907600)the 2115 Talent Development Program of China Agricultural University.
文摘Canopy photosynthesis,rather than leaf photosynthesis,is highly related to plant biomass and yield formation.Studying canopy photosynthesis and identifying the parameters that control it can help optimize agricultural management and achieve crop yield potential.Compared with traditional parameters,canopy occupation volume(COV)offers an integrative parameter on canopy architecture related to canopy photosynthetic rates.In this study,we developed a high-throughput method to derive COV for different rice varieties.We first used multi-perspective two-dimensional imaging to reconstruct three-dimensional point clouds of rice plants and developed a suite of pipelines to calculate plant height,leaf number,tiller number,and biomass,with R^(2) values of 91.8%,95.9%,82.3%,and 94.3%,respectively.We further employed point cloud data to reconstruct the surfaces of rice plants and construct a virtual canopy model of the rice population.Light distribution was simulated using a ray-tracing algorithm and canopy photosynthetic rates were simulated via photosynthetic rate-incident light intensity curve fitting.Furthermore,we systematically explored the relationships between canopy phenotypes and photosynthetic rates,and found that COV was the most effective predictor of canopy photosynthesis,achieving an R^(2) value of 92.1%.Adjustment in atmospheric transmittance showed that COV strongly correlated with canopy photosynthesis under different light conditions,with higher accuracy observed under diffuse light.Variations in planting density confirmed that this correlation remained strong at the community level.In summary,this study demonstrates that COV is closely linked to simulated canopy photosynthesis and the developed pipeline can support future agronomic and breeding research.
基金financially supported by the National Key R&D Program of China(2024YFD1200800)the Guangdong Basic and Applied Basic Research Foundation,China(2024A1515030094)。
文摘Highlights OsCAX2 is localized to tonoplast,and cadmium induces its expression.OsCAX2 overexpression reduces cadmium concentration in indica rice grains by 49.1%.Cadmium(Cd)exposure poses significant health risks to humans,and the International Agency for Research on Cancer has classified it as a Group I carcinogen.Cadmium undergoes minimal metabolism in the human body;consequently,prolonged Cd^(2+)exposure can cause severe damage to multiple organs including the liver,kidneys,lungs,bones,and immune system(Shao et al.2024).Rice,one of the three global staple crops,and Cd exposure in humans primarily occurs the consumption of contaminated rice grains.The contribution of rice to the total dietary Cd intake is over 50% for non-smoking Asian populations(Chen et al.2018;Shi et al.2020).
