Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types...Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.展开更多
Photoresponsive behavior and self assembly properties of three side chain azo polyelectrolytes, poly (2 (4 phenylazophenoxy) ethanol co acrylic acid) (PPAPE), poly (2 (4 (4′ nitrophenylazo) phenoxy) ethanol...Photoresponsive behavior and self assembly properties of three side chain azo polyelectrolytes, poly (2 (4 phenylazophenoxy) ethanol co acrylic acid) (PPAPE), poly (2 (4 (4′ nitrophenylazo) phenoxy) ethanol co acrylic acid) (PNAPE), and poly (2 (4 (4′ ethoxyphenylazo) phenoxy) ethanol co acrylic acid) (PEAPE), were studied. These polyelectrolytes with different degrees of functionalization of azo chromophores were fabricated into nano composite multilayers using two types of dipping solutions through a layer by layer electrostatic self assembling process. Results show that the ratio between tetrahydrofuran (THF) and H 2O significantly influences the photoresponsive behavior of PNAPE in THF H 2O mixture. The THF H 2O dipping solution, used in this work for self assembly of hydrophobic polyelectrolytes, is proved to be as applicable as aqueous dipping solution for normal self assembly of hydrophobic polyelectrolytes. However, significant differences in the multilayer growth between the two systems were also observed, which resulted from the remarkable difference of the existing forms of the polyelectrolytes in these two dipping solutions.展开更多
基金supported by the STI 2030—Major Projects 2021ZD0204000,No.2021ZD0204003 (to XZ)the National Natural Science Foundation of China,Nos.32170973 (to XZ),32071018 (to ZH)。
文摘Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.
基金the National Natural Science Foundationof China(No.5 992 5 30 9)
文摘Photoresponsive behavior and self assembly properties of three side chain azo polyelectrolytes, poly (2 (4 phenylazophenoxy) ethanol co acrylic acid) (PPAPE), poly (2 (4 (4′ nitrophenylazo) phenoxy) ethanol co acrylic acid) (PNAPE), and poly (2 (4 (4′ ethoxyphenylazo) phenoxy) ethanol co acrylic acid) (PEAPE), were studied. These polyelectrolytes with different degrees of functionalization of azo chromophores were fabricated into nano composite multilayers using two types of dipping solutions through a layer by layer electrostatic self assembling process. Results show that the ratio between tetrahydrofuran (THF) and H 2O significantly influences the photoresponsive behavior of PNAPE in THF H 2O mixture. The THF H 2O dipping solution, used in this work for self assembly of hydrophobic polyelectrolytes, is proved to be as applicable as aqueous dipping solution for normal self assembly of hydrophobic polyelectrolytes. However, significant differences in the multilayer growth between the two systems were also observed, which resulted from the remarkable difference of the existing forms of the polyelectrolytes in these two dipping solutions.
