Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive ...Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive appropriate treatment and the emergence of multidrug-resistant organisms could be prevented simultaneously.In this study,we have developed a microfluidic device named Self Dilution for Faster Antimicrobial Susceptibility Testing(SDFAST).This SlipChip-based device consists of two layers of microchips,allowing injection of bacterial suspension and antibiotics by simply connecting the two chips.By slipping one microchip against another in a single press of the microchip,the antibiotics can be diluted within seconds and be well mixed with bacterial samples.By combining SDFAST with a water-soluble tetrazolium salt-8(WST-8)assay,a range of clinically prevalent bacteria,including Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae,and Staphylococci species,were tested under various antibiotics.Color analysis after 4–6 h of incubation showed an abrupt change in the WST-8 color of certain wells with diluted antibiotics,proving that instrument-free and immediate identification of minimum inhibitory concentration(MIC)could be achieved.The testing on 51 clinical isolates had an agreement of 92%,proving the accuracy of our method.These results validated its advantages of simple operation,rapid testing,and low sample consumption comparing to conventional methods,which require 16–24 h of incubation.Therefore,our method shows great potential to be further developed into a medical instrument for automated medical testing and point-of-care diagnosis.展开更多
Due to the high viscosity of heavy crude oils,production from these reservoirs is a demanding task.To tackle this problem,reducing oil viscosity is a promising approach.There are various methods to reduce viscosity of...Due to the high viscosity of heavy crude oils,production from these reservoirs is a demanding task.To tackle this problem,reducing oil viscosity is a promising approach.There are various methods to reduce viscosity of heavy oil:heating,diluting,emulsification,and core annular flow.In this study,dilution approach was employed,using industrial solvents and gas condensate.The viscosity of two Iranian heavy crude oils was measured by mixing with solvents at different temperatures.Dilution of both oil samples with toluene and heptane,resulted in viscosity reduction.However,their effect became less significant at higher concentrations of diluent.Because of forming hydrogen bonds,adding methanol to heavy crude oil resulted in higher viscosity.By adding condensate,viscosity of each sample reduced.Gas condensate had a greater impact on heavier oil;however,at higher temperatures its effect was reduced.Diluting with naphtha decreased heavy oil viscosity in the same way as n-heptane and toluene.Besides experimental investigation,different viscosity models were evaluated for prediction of heavy oil/solvent viscosity.It was recognized that Lederer'model is the best one.展开更多
Active and poisoning-resistant Ru-based electrocatalysts for the hydrogen oxidation reaction(HOR)are designed and fabricated by integrating Cu/Ru dual single atoms and alloy CuRu nanoparticles(N-(CuRu)_(NP+SA)@NC)thro...Active and poisoning-resistant Ru-based electrocatalysts for the hydrogen oxidation reaction(HOR)are designed and fabricated by integrating Cu/Ru dual single atoms and alloy CuRu nanoparticles(N-(CuRu)_(NP+SA)@NC)through a strategy involving weak chemical reduction and ammonia-assisted gas-phase nitridation.The resultant N-(CuRu)_(NP+SA)@NC electrocatalysts feature nitrogen atoms coordinated to both Cu and Ru metal atoms via strong N-metal interactions.Density functional theory calculations revealed that alloyed CuRu nanoparticles and monodispersed Cu atoms are vital for altering the electronic configuration of the host Ru elements.This finely tuned structure enhanced the adsorption of H and OH and promoted CO oxidation over the N-(CuRu)_(NP+SA)@NC electrocatalyst,resulting in high alkaline HOR activity,as evidenced by the higher exchange current density of 3.74 mA cm^(-2)and high mass activity of 3.28 mAμg_(Ru)^(-1),which are far superior to those of most Ru-based catalysts reported to date.Moreover,the N-(CuRu)_(NP+SA)@NC electrocatalysts are resistant to CO poisoning and can be used at a high concentration of 1000 ppm CO with no distinct decay in the activity,in stark contrast to the commercial Pt/C catalyst under the same conditions.展开更多
In order to solve the problem of limited computational resources of multi-unmanned systems airborne navigation platform,a distributed cooperative positioning method based on confidence evaluation is proposed.Firstly,t...In order to solve the problem of limited computational resources of multi-unmanned systems airborne navigation platform,a distributed cooperative positioning method based on confidence evaluation is proposed.Firstly,the impact of ranging error,priori information,spatial geometric configuration and adjacent nodes count on cooperative positioning performance are analyzed individually.Secondly,a confidence evaluation method for measurement information of adjacent nodes is designed according to the cooperative positioning principle,which comprehensively considers the coupling relationship between influencing factors.Finally,a distributed cooperative navigation filter based on inter-vehicle ranging is designed.Simulation studies show that confidence evaluation method proposed in this paper can effectively characterize the contribution of measurement information to positioning results,and positioning accuracy under the proposed method is improved by more than 15%compared with the traditional screening methods based on optimal geometric configuration and closest distance.展开更多
The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizin...The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizing fertilization are crucial for sustainable agricultural production.A three-year field experiment was conducted to evaluate the effects of planting density on the critical nitrogen concentration dilution curve(CNDC)for spring maize under drip irrigation and fertilization integration,incorporating two planting densities:D1(60,000 plants ha^(-1))and D2(90,000 plants ha^(-1))and six nitrogen levels:no nitrogen(N0),90(N90),180(N180),270(N270),360(N360),and 450(N450)kg ha^(-1).A Bayesian hierarchical model was used to develop CNDC models based on dry matter(DM)and leaf area index(LAI).The results revealed that the critical nitrogen concentration exhibited a power function relationship with both DM and LAI,while planting density had no significant impact on the CNDC parameters.Based on these findings,we propose unified CNDC equations for maize under drip irrigation and fertilization integration:Nc=4.505DM-0.384(based on DM)and Nc=3.793LAI-0.327(based on LAI).Additionally,the nitrogen nutrition index(NNI),derived from the CNDC,increased with higher nitrogen application rates.The nitrogen nutrition index(NNI)approached 1 with a nitrogen application rate of 180 kg ha^(-1)under the D1 planting density,while it reached 1 at 270 kg ha^(-1)under the D2 planting density.The relationship between NNI and relative yield(RY)followed a“linear+plateau”model,with maximum RY observed when the NNI approached 1.Thus,under the condition of drip irrigation and fertilization integration in Northeast China’s spring maize production,the optimal nitrogen application rates for achieving the highest yields were 180 kg ha^(-1)at a planting density of 60,000 plants ha^(-1),and 270 kg ha^(-1)at a density of 90,000 plants ha^(-1).The CNDC and NNI models developed in this study are valuable tools for diagnosing nitrogen nutrition and guiding precise fertilization practices in maize production under integrated drip irrigation and fertilization systems in Northeast China.展开更多
To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperativ...To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperative tracking control method considering the effectiveness of passive detection. First, the system model of passive detection in UAV formation is constructed.Then, the Geometric Dilution of Precision(GDOP) of bearing-only sensor nodes pair on the observation plane is analyzed. Building on this foundation, the pairwise form is expanded to obtain the optimal geometric configuration for the entire network. Subsequently, the Distributed Cubature Information Filtering(DCIF) is integrated with the weighted average consensus protocol to design the distributed cooperative observer suitable for the system model, enabling state estimation of the target. Finally, within the distributed architecture, the Nonlinear Model Predictive Controller(NMPC) is designed. This controller autonomously assembles the UAV formation during the assembly phase and forms an optimal detection array. The UAV formation then tracks the target using the virtual geometric center based on the established rigid geometric configuration. The simulation experiments validate that the proposed model and method can enhance the passive detection effectiveness of the UAV formation, thereby achieving stable and efficient distributed cooperative tracking for the maneuvering target.展开更多
The radar radiation source signals hold extremely high reconnaissance value.Accurately positioning these signals constitutes one of the key technologies in safeguarding the security of the electromagnetic space.The po...The radar radiation source signals hold extremely high reconnaissance value.Accurately positioning these signals constitutes one of the key technologies in safeguarding the security of the electromagnetic space.The positioning error in multi-station scenarios is influenced not only by the accuracy of positioning parameter estimation but also by the geometric configuration of the positioning platform.This paper focuses on the direction of arrival(DOA),frequency difference of arrival(FDOA),and time difference of arrival(TDOA)methods,analyzing the optimal configuration,optimal detection area,and optimal position dilution of precision in both elevation-known and elevation-unknown scenarios.Specifically,the paper constructs a signal receiving model,establishes the corresponding positioning equations,and performs dimensional normalization on these equations to derive measurement values in meters.Through differential processing,the position dilution of precision is obtained,which is then used as the optimization function to determine the optimal configuration,optimal detection area,and optimal position dilution of precision.Simulation results validate the accuracy of the proposed formulas.展开更多
[ Objective] The aim was to study pretreatment of ultrasound enhancing dilute H2SO4 on cellulase activity of corn straw liquid fermentation and explore the pretretment＇ s optimal conditions. [ Method ] By using ortho...[ Objective] The aim was to study pretreatment of ultrasound enhancing dilute H2SO4 on cellulase activity of corn straw liquid fermentation and explore the pretretment＇ s optimal conditions. [ Method ] By using orthogonal test, the pretretment of ultrasound enhancing dilute H2SO4 on corn straw was studied, then straw was fermented as the sole carbon source. Finally, the cellulase activity in extracellular fermentation broth was determined. [Result] The results showed that cellulase activity in extracellular broth was greatest under the conditions of acid bath time 3 h, acid concentration 3.5%, ultrasonic power 150 W, and ultrasonic time 5 h. They were FPA 15.82 U/ml, Cx 39.9 U/ml, 13-Giu 55.94 U/ml respectively. [ Conclusion] Under the above conditions, extracellular cellulase production has a high stability.展开更多
A method for the determination of vitamin A in milk powder was developed by high performance liquid chromatography-isotope dilution mass spectrometry(HPLC-ID MS) and using deuterium-labeled vitamin A as an internal st...A method for the determination of vitamin A in milk powder was developed by high performance liquid chromatography-isotope dilution mass spectrometry(HPLC-ID MS) and using deuterium-labeled vitamin A as an internal standard.RSD of the method was 2.85%.This method was applied in the determination of standard reference material SRM 1849 with satisfactory results.展开更多
The mass of oligonucleotides was quantitated by liquid chromatography-isotope dilution mass spectrometry.The uncertainty was calculated.This could provide information for the further quantitative measurement and utili...The mass of oligonucleotides was quantitated by liquid chromatography-isotope dilution mass spectrometry.The uncertainty was calculated.This could provide information for the further quantitative measurement and utilization of DNA.展开更多
This article concentrates on the method of the determination of diazepam and chlorpromazine residue in meat and kidney,by using HPLC-MS/MS.The D6-chlorpromazine was added to sample as an internal standard.Then extract...This article concentrates on the method of the determination of diazepam and chlorpromazine residue in meat and kidney,by using HPLC-MS/MS.The D6-chlorpromazine was added to sample as an internal standard.Then extracted the sample with acetonitrile,cleaned up the extract through Oasis HLB cartridge.Determination and confirmation is made by means of liquid chromatography-tandem mass spectrometry,using internal standard method.The limit of quantitation of chlorpromazine and diazepam are all 1.0 μg/kg.展开更多
A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop...A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop） and reliability were selected as the optimization indicators to build a multi-objective optimization model to decide the optimum PLs location. A scheme was designed by establishing a four-dimensional model taking azimuth （a）, elevation angle （e） and epoch （t） of satellites as the input independent variables and Pdop as the dependent variable to calculate the optimum PLs location zone considering the real circumstances. And then the ultimate PLs location can be fixed by testing the curves of Pdop along time. A field collected Trimble R8 GPS data set in China University of Mining and Technology （CUMT） campus was used for the model test to show the effectiveness, and the proposed PLs optimum design scheme was used at the west open-pit mine of Fushun mining group Co., Ltd., in China, better Pdop and reliability have been achieved for the integration system. Both experiments show that the proposed scheme is excellent in designing GNSS/PLs system which is helpful for improving the performance of the positioning system and reducing the cost.展开更多
Microzoopiankton community composition in the north of South China Sea was investigated during autumn （between September and October）, 2004. Dilution technique using chlorophyll a （Chl a） was employed to estimate ...Microzoopiankton community composition in the north of South China Sea was investigated during autumn （between September and October）, 2004. Dilution technique using chlorophyll a （Chl a） was employed to estimate grazing rates and grazing pressure. The results showed that Polymenophorea Oligotrichida was the dominant group with 16 species, and there were 4 species in Oligotrichina and 11 species in Tintinnina. The ciliates abundance ranged from 9 to 102 ind/m^3, instantaneous growth rates of phytoplankton （k） varied from 0.03 / d to 2.13 / d. Grazing rates of microzooplankton（g） ranged between 0.01 / d and 1.06 / d. The grazing pressure on initial phytoplankton stock （Pi） and primary production （Pp） was 0.089 % - 65.23 % and 33.63 % - 86.04 %, respectively. The grazing of microzooplankton was mainly limited by ciliates abundance. Results of grazing pressure on primary production indicated that microzooplankton played an improtant role in transmitting primary production in the north of South China Sea.展开更多
[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam expl...[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.展开更多
Magnetism and the stability of (Ga 1-xFe x)As are investigated using the first principles LMTO-ASA band calculation by assuming supercell structures.Four concentrations of the 3d impurities are studied (x=1,1/2,1/...Magnetism and the stability of (Ga 1-xFe x)As are investigated using the first principles LMTO-ASA band calculation by assuming supercell structures.Four concentrations of the 3d impurities are studied (x=1,1/2,1/4,and 1/8).The results show the effect of varying Fe concentration on the magnetic and stable properties.展开更多
Platinum group elements (PGEs) includes platinum, palladium, iridium, osmium, rhodium and ruthenium. It has very high economic and scientific value in the field of geoscience and environmental science. But the analysi...Platinum group elements (PGEs) includes platinum, palladium, iridium, osmium, rhodium and ruthenium. It has very high economic and scientific value in the field of geoscience and environmental science. But the analysis data referred by the different lab are very disperse because of the difficulty of the determination of PGEs. It makes very difficult to fix the value of the PGEs in the standard reference materials. In the article, the values of the PGEs in the standard reference materials of ocean sediment are determined by isotope dilution technique and dependable values of these elements are provided.展开更多
The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs w...The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs was extracted from samples by Soxhlet extraction,concentrated and purified by FMS column chromatograph,carbon column enrichment.Confirmation and quantitative analysis at pg/g level of PCDD/Fs was performed by HRGC/HRMS using multiple ion detection mode(MID).TEQ concentration was calculated by WHO-TEF multiplying by concentration of seventeen PCDD/Fs congener.Median of PCDD/Fs concentration for fish,livestock,poultry,egg,vegetable oil,milk,vegetable totally 100 samples for ten diverse foods didn’t exceed the limit standards by EU.The level of PCDD/Fs for different food in the certain area was lower or comparable to the data reported by developed country in the world.The total weekly intake and monthly intake for local population or national population was 3.44,14.8 WHO-TEQ/kg BW and 1.5,6.42 pg WHO-TEQ/kg BW respectively,the value was lower than the Tolerable Weekly Intake of 14 pg WHO TEQ/kg BW for PCDDs,PCDFs and dioxin-like PCBs established by EU Scientific Committee for Food.The dietary PCDD/Fs intake for local people was higher than national population.And animal food was the dominant contributor to the total dietary intake,which accounted for more than 70 percent.These levels of consumption of diverse food containing typical levels of PCDD/Fs doesn’t present a risk to the health of the local population.But integrative dietary intake could be evaluated including of PCBs intake for population in the future.展开更多
The concept of metrological traceability in laboratory medicine has increasingly received considerable recognition worldwide.The Consultative Committee for Amount of Substance (CCQM) which concerned international comp...The concept of metrological traceability in laboratory medicine has increasingly received considerable recognition worldwide.The Consultative Committee for Amount of Substance (CCQM) which concerned international comparisons and establishment of international equivalence between national laboratories had organized international comparison CCQM-P77a: determination of serum cortisol in 2006.National Institute of Metrology (NIM) took part in the comparison as the representative of China and received good performance according to the results published by CCQM.展开更多
基金supported by the Health@InnoHK program of the Innovation and Technology Commission of the Hong Kong SAR Governmentsupported by The University of Hong Kong(202009185087)+1 种基金Collaborative Research Fund(C7165-20GF)General Research Fund(17307919 and 17303123)of the Research Grants Council of Hong Kong,Hong Kong.
文摘Antimicrobial resistance(AMR)is a global public health issue.Rapid and accurate antimicrobial susceptibility tests(AST)on bacteria isolates would facilitate appropriate choice of antibiotics,in which patients receive appropriate treatment and the emergence of multidrug-resistant organisms could be prevented simultaneously.In this study,we have developed a microfluidic device named Self Dilution for Faster Antimicrobial Susceptibility Testing(SDFAST).This SlipChip-based device consists of two layers of microchips,allowing injection of bacterial suspension and antibiotics by simply connecting the two chips.By slipping one microchip against another in a single press of the microchip,the antibiotics can be diluted within seconds and be well mixed with bacterial samples.By combining SDFAST with a water-soluble tetrazolium salt-8(WST-8)assay,a range of clinically prevalent bacteria,including Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae,and Staphylococci species,were tested under various antibiotics.Color analysis after 4–6 h of incubation showed an abrupt change in the WST-8 color of certain wells with diluted antibiotics,proving that instrument-free and immediate identification of minimum inhibitory concentration(MIC)could be achieved.The testing on 51 clinical isolates had an agreement of 92%,proving the accuracy of our method.These results validated its advantages of simple operation,rapid testing,and low sample consumption comparing to conventional methods,which require 16–24 h of incubation.Therefore,our method shows great potential to be further developed into a medical instrument for automated medical testing and point-of-care diagnosis.
文摘Due to the high viscosity of heavy crude oils,production from these reservoirs is a demanding task.To tackle this problem,reducing oil viscosity is a promising approach.There are various methods to reduce viscosity of heavy oil:heating,diluting,emulsification,and core annular flow.In this study,dilution approach was employed,using industrial solvents and gas condensate.The viscosity of two Iranian heavy crude oils was measured by mixing with solvents at different temperatures.Dilution of both oil samples with toluene and heptane,resulted in viscosity reduction.However,their effect became less significant at higher concentrations of diluent.Because of forming hydrogen bonds,adding methanol to heavy crude oil resulted in higher viscosity.By adding condensate,viscosity of each sample reduced.Gas condensate had a greater impact on heavier oil;however,at higher temperatures its effect was reduced.Diluting with naphtha decreased heavy oil viscosity in the same way as n-heptane and toluene.Besides experimental investigation,different viscosity models were evaluated for prediction of heavy oil/solvent viscosity.It was recognized that Lederer'model is the best one.
文摘Active and poisoning-resistant Ru-based electrocatalysts for the hydrogen oxidation reaction(HOR)are designed and fabricated by integrating Cu/Ru dual single atoms and alloy CuRu nanoparticles(N-(CuRu)_(NP+SA)@NC)through a strategy involving weak chemical reduction and ammonia-assisted gas-phase nitridation.The resultant N-(CuRu)_(NP+SA)@NC electrocatalysts feature nitrogen atoms coordinated to both Cu and Ru metal atoms via strong N-metal interactions.Density functional theory calculations revealed that alloyed CuRu nanoparticles and monodispersed Cu atoms are vital for altering the electronic configuration of the host Ru elements.This finely tuned structure enhanced the adsorption of H and OH and promoted CO oxidation over the N-(CuRu)_(NP+SA)@NC electrocatalyst,resulting in high alkaline HOR activity,as evidenced by the higher exchange current density of 3.74 mA cm^(-2)and high mass activity of 3.28 mAμg_(Ru)^(-1),which are far superior to those of most Ru-based catalysts reported to date.Moreover,the N-(CuRu)_(NP+SA)@NC electrocatalysts are resistant to CO poisoning and can be used at a high concentration of 1000 ppm CO with no distinct decay in the activity,in stark contrast to the commercial Pt/C catalyst under the same conditions.
基金supported in part by National Natural Science Foundation of China(Nos.62073163,62103285,62203228)National Defense Basic Research Program(No.JCKY2020605C009)+1 种基金Aeronautic Science Foundation of China(Nos.ASFC-2020Z071052001,202055052003)Foundation Strengthening Program Technology 173 Field Fund(No.2021-JCJQ-JJ-0308)。
文摘In order to solve the problem of limited computational resources of multi-unmanned systems airborne navigation platform,a distributed cooperative positioning method based on confidence evaluation is proposed.Firstly,the impact of ranging error,priori information,spatial geometric configuration and adjacent nodes count on cooperative positioning performance are analyzed individually.Secondly,a confidence evaluation method for measurement information of adjacent nodes is designed according to the cooperative positioning principle,which comprehensively considers the coupling relationship between influencing factors.Finally,a distributed cooperative navigation filter based on inter-vehicle ranging is designed.Simulation studies show that confidence evaluation method proposed in this paper can effectively characterize the contribution of measurement information to positioning results,and positioning accuracy under the proposed method is improved by more than 15%compared with the traditional screening methods based on optimal geometric configuration and closest distance.
基金supported by the grants from National Key Research and Development Program of China(2023YFD2303300)China Agriculture Research System(CARS-02-15)the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202004).
文摘The unreasonable application of nitrogen fertilizer poses a threat to agricultural productivity and the environment protection in Northeast China.Therefore,accurately assessing crop nitrogen requirements and optimizing fertilization are crucial for sustainable agricultural production.A three-year field experiment was conducted to evaluate the effects of planting density on the critical nitrogen concentration dilution curve(CNDC)for spring maize under drip irrigation and fertilization integration,incorporating two planting densities:D1(60,000 plants ha^(-1))and D2(90,000 plants ha^(-1))and six nitrogen levels:no nitrogen(N0),90(N90),180(N180),270(N270),360(N360),and 450(N450)kg ha^(-1).A Bayesian hierarchical model was used to develop CNDC models based on dry matter(DM)and leaf area index(LAI).The results revealed that the critical nitrogen concentration exhibited a power function relationship with both DM and LAI,while planting density had no significant impact on the CNDC parameters.Based on these findings,we propose unified CNDC equations for maize under drip irrigation and fertilization integration:Nc=4.505DM-0.384(based on DM)and Nc=3.793LAI-0.327(based on LAI).Additionally,the nitrogen nutrition index(NNI),derived from the CNDC,increased with higher nitrogen application rates.The nitrogen nutrition index(NNI)approached 1 with a nitrogen application rate of 180 kg ha^(-1)under the D1 planting density,while it reached 1 at 270 kg ha^(-1)under the D2 planting density.The relationship between NNI and relative yield(RY)followed a“linear+plateau”model,with maximum RY observed when the NNI approached 1.Thus,under the condition of drip irrigation and fertilization integration in Northeast China’s spring maize production,the optimal nitrogen application rates for achieving the highest yields were 180 kg ha^(-1)at a planting density of 60,000 plants ha^(-1),and 270 kg ha^(-1)at a density of 90,000 plants ha^(-1).The CNDC and NNI models developed in this study are valuable tools for diagnosing nitrogen nutrition and guiding precise fertilization practices in maize production under integrated drip irrigation and fertilization systems in Northeast China.
基金supported by the National Natural Science Foundation of China (Nos. 62176214 and 61973253)。
文摘To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperative tracking control method considering the effectiveness of passive detection. First, the system model of passive detection in UAV formation is constructed.Then, the Geometric Dilution of Precision(GDOP) of bearing-only sensor nodes pair on the observation plane is analyzed. Building on this foundation, the pairwise form is expanded to obtain the optimal geometric configuration for the entire network. Subsequently, the Distributed Cubature Information Filtering(DCIF) is integrated with the weighted average consensus protocol to design the distributed cooperative observer suitable for the system model, enabling state estimation of the target. Finally, within the distributed architecture, the Nonlinear Model Predictive Controller(NMPC) is designed. This controller autonomously assembles the UAV formation during the assembly phase and forms an optimal detection array. The UAV formation then tracks the target using the virtual geometric center based on the established rigid geometric configuration. The simulation experiments validate that the proposed model and method can enhance the passive detection effectiveness of the UAV formation, thereby achieving stable and efficient distributed cooperative tracking for the maneuvering target.
基金supported by the National Natural Science Foundation of China(Nos.62027801,62301035).
文摘The radar radiation source signals hold extremely high reconnaissance value.Accurately positioning these signals constitutes one of the key technologies in safeguarding the security of the electromagnetic space.The positioning error in multi-station scenarios is influenced not only by the accuracy of positioning parameter estimation but also by the geometric configuration of the positioning platform.This paper focuses on the direction of arrival(DOA),frequency difference of arrival(FDOA),and time difference of arrival(TDOA)methods,analyzing the optimal configuration,optimal detection area,and optimal position dilution of precision in both elevation-known and elevation-unknown scenarios.Specifically,the paper constructs a signal receiving model,establishes the corresponding positioning equations,and performs dimensional normalization on these equations to derive measurement values in meters.Through differential processing,the position dilution of precision is obtained,which is then used as the optimization function to determine the optimal configuration,optimal detection area,and optimal position dilution of precision.Simulation results validate the accuracy of the proposed formulas.
基金Supported by the National Key Technology R&D Program during the11~(th)five-year Plan(2007BAD34B03)the Important Project of Ministryof Education(107127)Scientific Research Foundation ofHefei University of Technology(113-036404)~~
文摘[ Objective] The aim was to study pretreatment of ultrasound enhancing dilute H2SO4 on cellulase activity of corn straw liquid fermentation and explore the pretretment＇ s optimal conditions. [ Method ] By using orthogonal test, the pretretment of ultrasound enhancing dilute H2SO4 on corn straw was studied, then straw was fermented as the sole carbon source. Finally, the cellulase activity in extracellular fermentation broth was determined. [Result] The results showed that cellulase activity in extracellular broth was greatest under the conditions of acid bath time 3 h, acid concentration 3.5%, ultrasonic power 150 W, and ultrasonic time 5 h. They were FPA 15.82 U/ml, Cx 39.9 U/ml, 13-Giu 55.94 U/ml respectively. [ Conclusion] Under the above conditions, extracellular cellulase production has a high stability.
文摘A method for the determination of vitamin A in milk powder was developed by high performance liquid chromatography-isotope dilution mass spectrometry(HPLC-ID MS) and using deuterium-labeled vitamin A as an internal standard.RSD of the method was 2.85%.This method was applied in the determination of standard reference material SRM 1849 with satisfactory results.
文摘The mass of oligonucleotides was quantitated by liquid chromatography-isotope dilution mass spectrometry.The uncertainty was calculated.This could provide information for the further quantitative measurement and utilization of DNA.
文摘This article concentrates on the method of the determination of diazepam and chlorpromazine residue in meat and kidney,by using HPLC-MS/MS.The D6-chlorpromazine was added to sample as an internal standard.Then extracted the sample with acetonitrile,cleaned up the extract through Oasis HLB cartridge.Determination and confirmation is made by means of liquid chromatography-tandem mass spectrometry,using internal standard method.The limit of quantitation of chlorpromazine and diazepam are all 1.0 μg/kg.
基金Project(2013RC16)supported by the Fundamental Research Funds for the Central Universities,China
文摘A new pseudolites （PLs） structure optimization model of global navigation satellite system （GNSS）/PLs integration positioning system used in deep open-pit mine was presented. Position dilution of precision （Pdop） and reliability were selected as the optimization indicators to build a multi-objective optimization model to decide the optimum PLs location. A scheme was designed by establishing a four-dimensional model taking azimuth （a）, elevation angle （e） and epoch （t） of satellites as the input independent variables and Pdop as the dependent variable to calculate the optimum PLs location zone considering the real circumstances. And then the ultimate PLs location can be fixed by testing the curves of Pdop along time. A field collected Trimble R8 GPS data set in China University of Mining and Technology （CUMT） campus was used for the model test to show the effectiveness, and the proposed PLs optimum design scheme was used at the west open-pit mine of Fushun mining group Co., Ltd., in China, better Pdop and reliability have been achieved for the integration system. Both experiments show that the proposed scheme is excellent in designing GNSS/PLs system which is helpful for improving the performance of the positioning system and reducing the cost.
文摘Microzoopiankton community composition in the north of South China Sea was investigated during autumn （between September and October）, 2004. Dilution technique using chlorophyll a （Chl a） was employed to estimate grazing rates and grazing pressure. The results showed that Polymenophorea Oligotrichida was the dominant group with 16 species, and there were 4 species in Oligotrichina and 11 species in Tintinnina. The ciliates abundance ranged from 9 to 102 ind/m^3, instantaneous growth rates of phytoplankton （k） varied from 0.03 / d to 2.13 / d. Grazing rates of microzooplankton（g） ranged between 0.01 / d and 1.06 / d. The grazing pressure on initial phytoplankton stock （Pi） and primary production （Pp） was 0.089 % - 65.23 % and 33.63 % - 86.04 %, respectively. The grazing of microzooplankton was mainly limited by ciliates abundance. Results of grazing pressure on primary production indicated that microzooplankton played an improtant role in transmitting primary production in the north of South China Sea.
基金Supported by National Basic Research Program of China(2006CB708407 2009CB220005)+2 种基金National Natural Science Foun-dation of China (90610001 20871106)Program of 211 Projectfor Zhengzhou University from Ministry of Education~~
文摘[Objective] This study was to explore the effects of dilute acid hydrolysis on fermentative biohydrogen production capacity of maize stalk. [Method] Using maize stalks subjected to mechanical disintegration,steam explosion and dilute acid hydrolysis as experimental materials,we measured and analyzed the effects of different treatments and particle size of maize stalk were analyzed. [Result] The optimal fermentative biohydrogen production was found under following parameters：pretreatment of 0.8% dilute H2SO4 following steam explosion,particle size of maize stalk of 0.425-0.850 mm,liquid-solid ratio [0.8% H2SO4 （M）：stalk （W）] of 10：1. [Conclusion] Post steam explosion,dilute 0.8% dilute H2SO4 intensified hydrolysis on maize stalk could produce fermentative biohydrogen production capacity.
文摘Magnetism and the stability of (Ga 1-xFe x)As are investigated using the first principles LMTO-ASA band calculation by assuming supercell structures.Four concentrations of the 3d impurities are studied (x=1,1/2,1/4,and 1/8).The results show the effect of varying Fe concentration on the magnetic and stable properties.
文摘Platinum group elements (PGEs) includes platinum, palladium, iridium, osmium, rhodium and ruthenium. It has very high economic and scientific value in the field of geoscience and environmental science. But the analysis data referred by the different lab are very disperse because of the difficulty of the determination of PGEs. It makes very difficult to fix the value of the PGEs in the standard reference materials. In the article, the values of the PGEs in the standard reference materials of ocean sediment are determined by isotope dilution technique and dependable values of these elements are provided.
文摘The status of PCDDs and PCDFs content in retail foods from a certain area by Isotope Dilution HRGC-HRMS was surveyed and the local population PCDD/Fs exposure from diverse foods and health risk was evaluated.PCDD/Fs was extracted from samples by Soxhlet extraction,concentrated and purified by FMS column chromatograph,carbon column enrichment.Confirmation and quantitative analysis at pg/g level of PCDD/Fs was performed by HRGC/HRMS using multiple ion detection mode(MID).TEQ concentration was calculated by WHO-TEF multiplying by concentration of seventeen PCDD/Fs congener.Median of PCDD/Fs concentration for fish,livestock,poultry,egg,vegetable oil,milk,vegetable totally 100 samples for ten diverse foods didn’t exceed the limit standards by EU.The level of PCDD/Fs for different food in the certain area was lower or comparable to the data reported by developed country in the world.The total weekly intake and monthly intake for local population or national population was 3.44,14.8 WHO-TEQ/kg BW and 1.5,6.42 pg WHO-TEQ/kg BW respectively,the value was lower than the Tolerable Weekly Intake of 14 pg WHO TEQ/kg BW for PCDDs,PCDFs and dioxin-like PCBs established by EU Scientific Committee for Food.The dietary PCDD/Fs intake for local people was higher than national population.And animal food was the dominant contributor to the total dietary intake,which accounted for more than 70 percent.These levels of consumption of diverse food containing typical levels of PCDD/Fs doesn’t present a risk to the health of the local population.But integrative dietary intake could be evaluated including of PCBs intake for population in the future.
文摘The concept of metrological traceability in laboratory medicine has increasingly received considerable recognition worldwide.The Consultative Committee for Amount of Substance (CCQM) which concerned international comparisons and establishment of international equivalence between national laboratories had organized international comparison CCQM-P77a: determination of serum cortisol in 2006.National Institute of Metrology (NIM) took part in the comparison as the representative of China and received good performance according to the results published by CCQM.
