In this review,we propose a comprehensive overview of additive manufacturing(AM)technologies and design possibilities in manufacturing metamaterials for various applications in the biomedical field,of which many are i...In this review,we propose a comprehensive overview of additive manufacturing(AM)technologies and design possibilities in manufacturing metamaterials for various applications in the biomedical field,of which many are inspired by nature itself.It describes how new AM technologies(e.g.continuous liquid interface production and multiphoton polymerization,etc)and recent developments in more mature AM technologies(e.g.powder bed fusion,stereolithography,and extrusion-based bioprinting(EBB),etc)lead to more precise,efficient,and personalized biomedical components.EBB is a revolutionary topic creating intricate models with remarkable mechanical compatibility of metamaterials,for instance,stress elimination for tissue engineering and regenerative medicine,negative or zero Poisson’s ratio.By exploiting the designs of porous structures(e.g.truss,triply periodic minimal surface,plant/animal-inspired,and functionally graded lattices,etc),AM-made bioactive bone implants,artificial tissues,and organs are made for tissue replacement.The material palette of the AM metamaterials has high diversity nowadays,ranging from alloys and metals(e.g.cobalt-chromium alloys and titanium,etc)to polymers(e.g.biodegradable polycaprolactone and polymethyl methacrylate,etc),which could be even integrated within bioactive ceramics.These advancements are driving the progress of the biomedical field,improving human health and quality of life.展开更多
Due to advantages of high power-conversion efficiency(PCE), large power-to-weight ratio(PWR), low cost and solution processibility, flexible perovskite solar cells(f-PSCs) have attracted extensive attention in recent ...Due to advantages of high power-conversion efficiency(PCE), large power-to-weight ratio(PWR), low cost and solution processibility, flexible perovskite solar cells(f-PSCs) have attracted extensive attention in recent years. The PCE of f-PSCs has developed rapidly to over 25%, showing great application prospects in aerospace and wearable electronic devices. This review systematically sorts device structures and compositions of f-PSCs, summarizes various methods to improve its efficiency and stability recent years. In addition, the applications and potentials of f-PSCs in space vehicle and aircraft was discussed. At last, we prospect the key scientific and technological issues that need to be addressed for f-PSCs at current stage.展开更多
The application of digital technology in prosthodontics not only improves the accuracy of restoration and the efficiency of diagnosis and treatment,but also brings more comfortable and convenient diagnosis and treatme...The application of digital technology in prosthodontics not only improves the accuracy of restoration and the efficiency of diagnosis and treatment,but also brings more comfortable and convenient diagnosis and treatment experience to patients.It effectively promotes the rapid development of the prosthodontic aesthetics industry and leads prosthodontic technology to gradually enter a new digital era.Combining the advantages of digital technology in prosthodontics,this paper focuses on the specific applications of digital impression technology,CAD/CAM technology,network-based integrated cloud service technology,and CBCT technology in prosthodontics.On this basis,it prospects the development trend of digital technology in the field of prosthodontics,aiming to provide solid theoretical and practical support for clinical practice of prosthodontics and promote the gradual integration of the prosthodontic industry with the digital era.展开更多
High flux reactors(HFRs)are a special type of research reactor aimed at providing a high neutron flux.Compared with power reactors and other research reactors,HFRs have unique technical features in terms of reactor co...High flux reactors(HFRs)are a special type of research reactor aimed at providing a high neutron flux.Compared with power reactors and other research reactors,HFRs have unique technical features in terms of reactor core design,irradiation capability,and operating characteristics.They can be applied to the irradiation tests of nuclear fuels and materials,radioisotope production,neutron science,and experiments.This paper reviews HFRs,including their development history,technical features,and application areas,as well as trends in the development of new and advanced HFRs.展开更多
In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementi...In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementing robust security measures in modern web applications and the proof of the Methodology applied to Vue.js,Spring Boot,and MySQL architecture.The proposed approach addresses critical security challenges through a multi-layered framework that encompasses essential security dimensions including multi-factor authentication,fine-grained authorization controls,sophisticated session management,data confidentiality and integrity protection,secure logging mechanisms,comprehensive error handling,high availability strategies,advanced input validation,and security headers implementation.Significant contributions are made to the field of web application security.First,a detailed catalogue of security requirements specifically tailored to protect web applications against contemporary threats,backed by rigorous analysis and industry best practices.Second,the methodology is validated through a carefully designed proof-of-concept implementation in a controlled environment,demonstrating the practical effectiveness of the security measures.The validation process employs cutting-edge static and dynamic analysis tools for comprehensive dependency validation and vulnerability detection,ensuring robust security coverage.The validation results confirm the prevention and avoidance of security vulnerabilities of the methodology.A key innovation of this work is the seamless integration of DevSecOps practices throughout the secure Software Development Life Cycle(SSDLC),creating a security-first mindset from initial design to deployment.By combining proactive secure coding practices with defensive security approaches,a framework is established that not only strengthens application security but also fosters a culture of security awareness within development teams.This hybrid approach ensures that security considerations are woven into every aspect of the development process,rather than being treated as an afterthought.展开更多
The research and development of new traditional Chinese medicine(TCM)drugs have progressively established a novel system founded on the integration of TCM theory,human experience,and clinical trials(termed the“Three ...The research and development of new traditional Chinese medicine(TCM)drugs have progressively established a novel system founded on the integration of TCM theory,human experience,and clinical trials(termed the“Three Combinations”).However,considering TCM's distinctive features of“syndrome differentiation and treatment”and“multicomponent formulations and complex mechanisms”,current TCM drug development faces challenges such as insufficient understanding of the material basis and the overall mechanism of action and an incomplete evidence chain system.Moreover,significant obstacles persist in gathering human experience data,evaluating clinical efficacy,and controlling the quality of active ingredients,which impede the innovation process in TCM drug development.Network pharmacology,centered on the“network targets”theory,transcends the limitations of the conventional“single target”reductionist research model.It emphasizes the comprehensive effects of disease or syndrome biological networks as targets to elucidate the overall regulatory mechanism of TCM prescriptions.This approach aligns with the holistic perspective of TCM,offering a novel method consistent with TCM's holistic view for investigating the complex mechanisms of TCM and developing new TCM drugs.It is internationally recognized as a“next-generation drug research model”.To advance the research of new tools,methods,and standards for TCM evaluation and to overcome fundamental,critical,and cutting-edge technical challenges in TCM regulation,this consensus aims to explore the characteristics,progress,challenges,applicable pathways,and specific applications of network pharmacology as a new theory,method,and tool in TCM drug development.The goal is to enhance the quality of TCM drug research and development and accelerate the efficiency of developing new TCM products.展开更多
Fracability evaluation is critical for efficiently extracting deep shale gas using hydraulic fracturing to avoid blind drilling and fracking.However,existing fracability indices often fail to systematically consider t...Fracability evaluation is critical for efficiently extracting deep shale gas using hydraulic fracturing to avoid blind drilling and fracking.However,existing fracability indices often fail to systematically consider the mechanical behavior of rocks at high temperatures and high pressures(HTHP),coupled with geostress distributions and heterogeneous reservoir characteristics.This critical omission limits their effectiveness in accurately identifying the optimal fracability sweet spots within deep reservoirs.In this work,a fracability evaluation model was proposed based on the combined weighting method,integrating the improved brittleness index,rock strength,geostresses and natural weakness characteristics.A fracability grading evaluation was carried out to determine the potential fracture characteristics corresponding to shales with different fracability levels.Additionally,the fracability index was used for field validation and applications.Results show that rock brittleness and fracability are not equivalent for deep reservoirs.The fracability index is closely related to the pay zones and actual gas production,with a correlation as high as 84%,implying that the proposed method has practical significance in both experimental and field applications.The above findings can provide theoretical guidance for the selection of fracturing candidates and the optimal design of fracturing in deep resource development.展开更多
Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation s...Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation strategies are widely recommended to enhance water use efficiency,knowledge remains limited regarding their interactions with soil amendments such as biochar and the consequent impacts on reproductive traits.This study aimed to evaluate the combined effects of deficit irrigation strategies and biochar application on pollen viability and morphology in Capsicum annuum.The experiment was conducted under full,partial,and deficit irrigation regimes with and without biochar treatment,following a randomized block design.The primary parameters examined were pollen viability(viable,semi-viable,and non-viable rates),anther width and length,and pollen width and length.Microscopic measurements and statistical analyses(p≤0.05)revealed significant effects of both irrigation regimes and biochar applications.Under deficit irrigation,viable,semi-viable,and non-viable pollen rates were 29.84%,32.95%,and 37.21%,respectively,whereas the highest viable pollen rate was observed under full irrigation.In partial irrigation,viable pollen accounted for 31.67%,semi-viable for 38.81%,and non-viable for 29.49%.In plots treated with biochar under partial irrigation,anther width(1700.89μm),anther length(3805.34μm),pollen width(26.93μm),and pollen length(37.42μm)reached the highest values,while the lowest values were recorded in deficit irrigation plots without biochar.These findings emphasize the importance of integrating biochar into irrigation management to mitigate the adverse effects of water stress on pollen development.Nevertheless,further research is needed to clarify the long-term implications of these practices for reproductive success and agricultural sustainability.展开更多
Existing creep constitutive models rarely incorporate studies on the coupling mechanism between creep damage and rock strain softening/hardening.This study analyzed the strain softening and hardening behaviors of argi...Existing creep constitutive models rarely incorporate studies on the coupling mechanism between creep damage and rock strain softening/hardening.This study analyzed the strain softening and hardening behaviors of argillaceous sandstone and sandy mudstone during load-induced failure based on the plastic increment theory.These behaviors were then coupled with an improved Burgers creep model to establish a coupled creep-damage and plastic softening/hardening model.Finally,the validity and engineering applicability of the proposed model were verified through FLAC~(3D)numerical simulations.The numerical simulation results of standard cylindrical specimens show that the established coupling model can effectively reflect the unloading creep deformation law and failure characteristics of argillaceous sandstone and sandy mudstone.Taking the diversion tunnel of a hydropower station in Northwest China as an example for engineering application,the coupled creep-damage and plastic softening/hardening model is introduced into FLAC~(3D)to carry out numerical simulation calculation of the tunnel under excavation and unsupported creep conditions.The results show that the uncoordinated deformation of the upper and lower walls of the surrounding rock of the tunnel is more prominent.When the buried depth of the tunnel increases to 80 m,the monitoring point C in the sandy mudstone area of the upper wall shows nonlinear accelerated deformation under unsupported creep conditions,and the maximum displacement in the horizontal direction reaches 44.5 mm,and the maximum displacement in the vertical direction reaches 53.5 mm.The coupled creep-damage and plastic softening/hardening model established in the research results can well describe the whole process of uncoordinated deformation and failure in the unloading creep process of soft-hard interbedded rock mass.展开更多
Affecting about 241.4 million people worldwide,Schistosoma(S.)mansoni,the main etiological agent of intestinal schistosomiasis,is one of the most prevalent diseases in the world remaining a public health issue,especia...Affecting about 241.4 million people worldwide,Schistosoma(S.)mansoni,the main etiological agent of intestinal schistosomiasis,is one of the most prevalent diseases in the world remaining a public health issue,especially in developing countries in South America,Africa and the Caribbean.In Brazil,more than 30 million people are at risk of infection,and nearly 1.5 million are infected by S.mansoni[1].Although the implemented control measures proved to be efficient and contributed to a decrease in morbidity and mortality associated with S.mansoni infections,they led to a new epidemiological scenario characterized by low-intensity infections and areas with low prevalence,presenting a new challenge towards the elimination and control of schistosomiasis[2].展开更多
The actively heated fiber-optic(AHFO)technology has emerged as a frontier and hotspot in soil water content measurement,offering advantages such as easy installation,large-scale distributed measurement capability,and ...The actively heated fiber-optic(AHFO)technology has emerged as a frontier and hotspot in soil water content measurement,offering advantages such as easy installation,large-scale distributed measurement capability,and resistance to electromagnetic interference.However,current AHFO water content sensors fail to simultaneously achieve high precision,applicability for deep soil,and automated real-time monitoring,thereby limiting their development and application.Therefore,this study introduces a novel actively heated fiber Bragg grating(AH-FBG)cable.Laboratory tests were conducted to assess the heating uniformity of the AH-FBG cable and to establish the temperature characteristic value(T_(t))-soil water content(θ)calibration formula for water content measurement.Subsequently,AH-FBG cables were deployed for in situ soil water content monitoring in a test pit on the Loess Plateau.Through two-year monitoring data verified the accuracy of the AH-FBG cable and elucidated the spatiotemporal distribution of in situ loess water content.Laboratory results demonstrated superior heating uniformity of AHFBG cable,with a T_(t) standard deviation of approximately 0.3℃.In the field,the AH-FBG cable exhibited excellent performance in soil water content measurement,achieving a high accuracy of 0.023 cm^(3)/cm^(3).Further analysis revealed that the θ fluctuation predominantly occurred within a 10 m depth from the soil surface,with an overall upward trend over the two-year monitoring period;the response of shallow θ to precipitation was significant but exhibited increasing hysteresis with depth;frequent precipitation significantly enhanced water infiltration depth.This study provides technical guidance for highprecision,quasi-distributed,automated and real-time water content measurement of deep soil.展开更多
Based on the technological demands for significantly enhancing oil recovery and long-term CO_(2)sequestration in the lacustrine oil reservoirs of China,this study systematically reviews the progress and practices of C...Based on the technological demands for significantly enhancing oil recovery and long-term CO_(2)sequestration in the lacustrine oil reservoirs of China,this study systematically reviews the progress and practices of CO_(2)flooding and storage technologies in recent years.It addresses the key technological needs and challenges faced in scaling up the application of CO_(2)flooding and storage to mature,developed oil fields,and analyzes future development directions.During the pilot test phase(2006-2019),continuous development and application practices led to the establishment of the first-generation CO_(2)flooding and storage technology system for lacustrine reservoirs.In the industrialization phase(since 2020),significant advances and insights have been achieved in terms of confined phase behavior,storage mechanisms,reservoir engineering,sweep control,engineering process and storage monitoring,enabling the maturation of the second-generation CO_(2)flooding and storage theories and technologies to effectively support the demonstration projects of Carbon Capture,Utilization and Storage(CCUS).To overcome key technical issues such as low miscibility,difficulty in gas channeling control,high process requirements,limited application scenarios,and coordination challenges in CO_(2)flooding and storage,and to support the large-scale application of CCUS,it is necessary to strengthen research on key technologies for establishing the third-generation CO_(2)flooding and storage technological system incorporating miscibility enhancement and transformation,comprehensive regulation for sweep enhancement,whole-process engineering techniques and equipment,long-term storage monitoring safety,and synergistic optimization of flooding and storage.展开更多
Nitrogen oxides(NO_(x))are crucial in tropospheric photochemical ozone(O_(3))production and oxidation capacity.Currently,the widely used NO_(x)measurement technique is chemiluminescence(CL)(CL-NO_(x)),which tends to o...Nitrogen oxides(NO_(x))are crucial in tropospheric photochemical ozone(O_(3))production and oxidation capacity.Currently,the widely used NO_(x)measurement technique is chemiluminescence(CL)(CL-NO_(x)),which tends to overestimate NO_(2)due to atmospheric oxidation products of NO_(x)(i.e.,NO_(z)).We developed and characterized a NO_(x)measurement system using the cavity attenuated phase shift(CAPS)technique(CAPS-NO_(x)),which is free from interferences with nitrogen-containing species.The NO_(x)measured by the CAPS-NO_(x)and CL-NO_(x)analyzers were compared.Results show that both analyzers showed consistent measurement results for NO,but the NO_(2)measured by the CAPS-NO_(x)analyzer(NO_(2)_CAPS)was mostly lower than that measured by the CL-NO_(x)analyzer(NO_(2)_CL),which led to the deviations in O_(3)formation sensitivity regime and O_(x)(=O_(3)+NO_(2))sources(i.e.,regional background and photochemically produced O_(x))determined by the ozone production efficiencies(OPE)calculated from NO_(2)_CL and NO_(2)_CAPS.Overall,OPE_CL exceeded OPE_CAPS by 18.9%,which shifted 3 out of 13 observation days from the VOCs-limited to the transition regime when judging using OPE_CL,as compared to calculations using OPE_CAPS.During the observation period,days dominated by regional background O_(x)accounted for 46%and 62%when determined using NO_(2)_CL and NO_(2)_CAPS,respectively.These findings suggest that the use of the CL-NO_(x)analyzer tends to underestimate both the VOCs-limited regime and the regional background O_(x)dominated days.The newly built CAPS-NO_(x)analyzer here can promote the accurate measurement of NO_(2),which is meaningful for diagnosing O_(3)formation regimes and O_(x)sources.展开更多
α-Trifluoromethyl ketones are a class of useful compounds with versatile applications.Their synthetic application via the transformation of the C—F bonds is of particular interest by allowing the synthesis of organi...α-Trifluoromethyl ketones are a class of useful compounds with versatile applications.Their synthetic application via the transformation of the C—F bonds is of particular interest by allowing the synthesis of organic compounds with diverse structures.Herein,the advances in the research areas ofα-trifluoromethyl ketone synthesis and their defluorination reactions are reviewed.Discussion on the mechanisms of the typical reactions has also been provided,in hope of affording some guides to the chemistry ofα-trifluoromethyl ketones in the synthetic methods toward themselves and their derivatives.展开更多
Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-li...Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.展开更多
Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehens...Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehensive review of commonly employed coalbed methane extraction technologies.It then delves into several critical issues in the current stage of CBM exploration and development in China,including the compatibility of existing technologies with CBM reservoirs,the characteristics and occurrence states of CBM reservoirs,critical desorption pressure,and gas generation mechanisms.Our research indicates that current CBM exploration and development technologies in China have reached an internationally advanced level,yet the industry is facing unprecedented challenges.Despite progress in low-permeability,high-value coal seams,significant breakthroughs have not been achieved in exploring other types of coal seams.For different coal reservoirs,integrated extraction technologies have been developed,such as surface pre-depressurisation and segmented hydraulic fracturing of coal seam roof strata.Additionally,techniques like large-scale volume fracturing in horizontal wells have been established,significantly enhancing reservoir stimulation effects and coalbed methane recovery rates.However,all of these technologies are fundamentally based on permeation.These technologies lack direct methods aimed at enhancing the diffusion rate of CBM,thereby failing to fully reflect the unique characteristics of CBM.Current CBM exploration and development theories and technologies are not universally applicable to all coal seams.They do not adequately account for the predominantly adsorbed state of CBM,and the complex and variable gas generation mechanisms further constrain CBM development in China.Finally,continuous exploration of new deep CBM exploration technologies is necessary.Integrating more effective reservoir stimulation technologies is essential to enhance technical adaptability concerning CBM reservoir characteristics,gas occurrence states,and gas generation mechanisms,ultimately achieving efficient CBM development.We conclude that while China possesses a substantial foundation of deep fractured CBM resources,industry development is constrained and requires continuous exploration of new CBM exploration and development technologies to utilize these resources effectively.展开更多
Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vi...Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vironment and intensify carbon emissions.However,the use of microbially induced calcium carbonate pre-cipitation(MICP)to obtain bio-cement is a novel technique with the potential to induce soil stability,providing a low-carbon,environment-friendly,and sustainable integrated solution for some geotechnical engineering pro-blems in the environment.This paper presents a comprehensive review of the latest progress in soil improvement based on the MICP strategy.It systematically summarizes and overviews the mineralization mechanism,influ-encing factors,improved methods,engineering characteristics,and current field application status of the MICP.Additionally,it also explores the limitations and correspondingly proposes prospective applications via the MICP approach for soil improvement.This review indicates that the utilization of different environmental calcium-based wastes in MICP and combination of materials and MICP are conducive to meeting engineering and market demand.Furthermore,we recommend and encourage global collaborative study and practice with a view to commercializing MICP technique in the future.The current review purports to provide insights for engineers and interdisciplinary researchers,and guidance for future engineering applications.展开更多
The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established b...The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established by the World Health Organization(WHO). Reform in China has demonstrated the unwavering confidence and utmost determination of the Chinese government and the Chinese transplantation community. The year 2015 marked a historic turning point when voluntary donations from Chinese citizens became the sole legitimate source for organ transplantation. Since 2015, China has gradually established and refined the “Chinese Mode” and “China System” for organ donation and transplantation, fulfilling its political pledge of reform, and has garnered international recognition, and fostered a social culture which promotes organ donation. This article reviewed the history of reform on organ donation and transplantation in China, presented a new pattern of establishment of organ donation system in the new era of the country, and the direction of advances in the future.展开更多
Liquid metals(LMs),because of their ability to remain in a liquid state at room temperature,render them highly versatile for applications in electronics,energy storage,medicine,and robotics.Among various LMs,Ga-based ...Liquid metals(LMs),because of their ability to remain in a liquid state at room temperature,render them highly versatile for applications in electronics,energy storage,medicine,and robotics.Among various LMs,Ga-based LMs exhibit minimal cytotoxicity,low viscosity,high thermal and electrical conductivities,and excellent wettability.Therefore,Ga-based LM composites(LMCs)have emerged as a recent research focus.Recent advancements have focused on novel fabrication techniques and applications spanning energy storage,flexible electronics,and biomedical devices.Particularly noteworthy are the developments in wearable sensors and electronic skins,which hold promise for healthcare monitoring and human-machine interfaces.Despite their potential,challenges,such as oxidative susceptibil-ity and biocompatibility,remain.Creating bio-based LMC materials is a promising approach to address these issues while exploring new avenues to optimize LMC performance and broaden its application domains.This review provides a concise overview of the recent trends in LMC research,highlights their transformative impacts,and outlines key directions for future investigation and development.展开更多
基金sponsored by the Science and Technology Program of Hubei Province,China(2022EHB020,2023BBB096)support provided by Centre of the Excellence in Production Research(XPRES)at KTH。
文摘In this review,we propose a comprehensive overview of additive manufacturing(AM)technologies and design possibilities in manufacturing metamaterials for various applications in the biomedical field,of which many are inspired by nature itself.It describes how new AM technologies(e.g.continuous liquid interface production and multiphoton polymerization,etc)and recent developments in more mature AM technologies(e.g.powder bed fusion,stereolithography,and extrusion-based bioprinting(EBB),etc)lead to more precise,efficient,and personalized biomedical components.EBB is a revolutionary topic creating intricate models with remarkable mechanical compatibility of metamaterials,for instance,stress elimination for tissue engineering and regenerative medicine,negative or zero Poisson’s ratio.By exploiting the designs of porous structures(e.g.truss,triply periodic minimal surface,plant/animal-inspired,and functionally graded lattices,etc),AM-made bioactive bone implants,artificial tissues,and organs are made for tissue replacement.The material palette of the AM metamaterials has high diversity nowadays,ranging from alloys and metals(e.g.cobalt-chromium alloys and titanium,etc)to polymers(e.g.biodegradable polycaprolactone and polymethyl methacrylate,etc),which could be even integrated within bioactive ceramics.These advancements are driving the progress of the biomedical field,improving human health and quality of life.
基金supported by National Natural Science Foundation of China (Grant Nos. 62204104, 42005138, 12274190, 12274189, 62275115)Shandong Province High Education Youth Innovation Team Program (Grant No. 2023KJ210)Science and Technology Program of Yantai (Grant No. 2023JCYJ047)。
文摘Due to advantages of high power-conversion efficiency(PCE), large power-to-weight ratio(PWR), low cost and solution processibility, flexible perovskite solar cells(f-PSCs) have attracted extensive attention in recent years. The PCE of f-PSCs has developed rapidly to over 25%, showing great application prospects in aerospace and wearable electronic devices. This review systematically sorts device structures and compositions of f-PSCs, summarizes various methods to improve its efficiency and stability recent years. In addition, the applications and potentials of f-PSCs in space vehicle and aircraft was discussed. At last, we prospect the key scientific and technological issues that need to be addressed for f-PSCs at current stage.
基金Wuhan University of Arts and Science 2023 University-Level Scientific Research(Project No.:2023xk33)。
文摘The application of digital technology in prosthodontics not only improves the accuracy of restoration and the efficiency of diagnosis and treatment,but also brings more comfortable and convenient diagnosis and treatment experience to patients.It effectively promotes the rapid development of the prosthodontic aesthetics industry and leads prosthodontic technology to gradually enter a new digital era.Combining the advantages of digital technology in prosthodontics,this paper focuses on the specific applications of digital impression technology,CAD/CAM technology,network-based integrated cloud service technology,and CBCT technology in prosthodontics.On this basis,it prospects the development trend of digital technology in the field of prosthodontics,aiming to provide solid theoretical and practical support for clinical practice of prosthodontics and promote the gradual integration of the prosthodontic industry with the digital era.
文摘High flux reactors(HFRs)are a special type of research reactor aimed at providing a high neutron flux.Compared with power reactors and other research reactors,HFRs have unique technical features in terms of reactor core design,irradiation capability,and operating characteristics.They can be applied to the irradiation tests of nuclear fuels and materials,radioisotope production,neutron science,and experiments.This paper reviews HFRs,including their development history,technical features,and application areas,as well as trends in the development of new and advanced HFRs.
文摘In today’s rapidly evolving digital landscape,web application security has become paramount as organizations face increasingly sophisticated cyber threats.This work presents a comprehensive methodology for implementing robust security measures in modern web applications and the proof of the Methodology applied to Vue.js,Spring Boot,and MySQL architecture.The proposed approach addresses critical security challenges through a multi-layered framework that encompasses essential security dimensions including multi-factor authentication,fine-grained authorization controls,sophisticated session management,data confidentiality and integrity protection,secure logging mechanisms,comprehensive error handling,high availability strategies,advanced input validation,and security headers implementation.Significant contributions are made to the field of web application security.First,a detailed catalogue of security requirements specifically tailored to protect web applications against contemporary threats,backed by rigorous analysis and industry best practices.Second,the methodology is validated through a carefully designed proof-of-concept implementation in a controlled environment,demonstrating the practical effectiveness of the security measures.The validation process employs cutting-edge static and dynamic analysis tools for comprehensive dependency validation and vulnerability detection,ensuring robust security coverage.The validation results confirm the prevention and avoidance of security vulnerabilities of the methodology.A key innovation of this work is the seamless integration of DevSecOps practices throughout the secure Software Development Life Cycle(SSDLC),creating a security-first mindset from initial design to deployment.By combining proactive secure coding practices with defensive security approaches,a framework is established that not only strengthens application security but also fosters a culture of security awareness within development teams.This hybrid approach ensures that security considerations are woven into every aspect of the development process,rather than being treated as an afterthought.
基金supported by the National Medical Products Administration Commissioned Research Project (No.20211440216)the National Administration of Traditional Chinese Medicine Science and Technology Project (No.GZY-KJS-2024-03)+3 种基金the State Key Laboratory of Drug Regulatory Science Project (No.2023SKLDRS0104)the Basic Research Program Natural Science Fund-Frontier Leading Technology Basic Research Special Project of Jiangsu Province (No.BK20232014)the Programs Foundation for Leading Talents in National Administration of Traditional Chinese Medicine of China“Qihuang scholars”Projectthe Tianjin Administration for Market Regulation Science and Technology Key Projects (No.2022-W35)。
文摘The research and development of new traditional Chinese medicine(TCM)drugs have progressively established a novel system founded on the integration of TCM theory,human experience,and clinical trials(termed the“Three Combinations”).However,considering TCM's distinctive features of“syndrome differentiation and treatment”and“multicomponent formulations and complex mechanisms”,current TCM drug development faces challenges such as insufficient understanding of the material basis and the overall mechanism of action and an incomplete evidence chain system.Moreover,significant obstacles persist in gathering human experience data,evaluating clinical efficacy,and controlling the quality of active ingredients,which impede the innovation process in TCM drug development.Network pharmacology,centered on the“network targets”theory,transcends the limitations of the conventional“single target”reductionist research model.It emphasizes the comprehensive effects of disease or syndrome biological networks as targets to elucidate the overall regulatory mechanism of TCM prescriptions.This approach aligns with the holistic perspective of TCM,offering a novel method consistent with TCM's holistic view for investigating the complex mechanisms of TCM and developing new TCM drugs.It is internationally recognized as a“next-generation drug research model”.To advance the research of new tools,methods,and standards for TCM evaluation and to overcome fundamental,critical,and cutting-edge technical challenges in TCM regulation,this consensus aims to explore the characteristics,progress,challenges,applicable pathways,and specific applications of network pharmacology as a new theory,method,and tool in TCM drug development.The goal is to enhance the quality of TCM drug research and development and accelerate the efficiency of developing new TCM products.
基金supported by the National Natural Science Foun-dation of China(Nos.U24B2035 and U22A20166)the Postdoctoral Fellowship Program and China Postdoctoral Science Foundation(Nos.BX20250034 and 2024M763503)the Natural Science Foundation of Hubei Province of China(No.2024AFD374).
文摘Fracability evaluation is critical for efficiently extracting deep shale gas using hydraulic fracturing to avoid blind drilling and fracking.However,existing fracability indices often fail to systematically consider the mechanical behavior of rocks at high temperatures and high pressures(HTHP),coupled with geostress distributions and heterogeneous reservoir characteristics.This critical omission limits their effectiveness in accurately identifying the optimal fracability sweet spots within deep reservoirs.In this work,a fracability evaluation model was proposed based on the combined weighting method,integrating the improved brittleness index,rock strength,geostresses and natural weakness characteristics.A fracability grading evaluation was carried out to determine the potential fracture characteristics corresponding to shales with different fracability levels.Additionally,the fracability index was used for field validation and applications.Results show that rock brittleness and fracability are not equivalent for deep reservoirs.The fracability index is closely related to the pay zones and actual gas production,with a correlation as high as 84%,implying that the proposed method has practical significance in both experimental and field applications.The above findings can provide theoretical guidance for the selection of fracturing candidates and the optimal design of fracturing in deep resource development.
文摘Water scarcity is an escalating global challenge that severely threatens productivity and reproductive success in crops,particularly in drought-sensitive species such as Capsicum annuum L.Although deficit irrigation strategies are widely recommended to enhance water use efficiency,knowledge remains limited regarding their interactions with soil amendments such as biochar and the consequent impacts on reproductive traits.This study aimed to evaluate the combined effects of deficit irrigation strategies and biochar application on pollen viability and morphology in Capsicum annuum.The experiment was conducted under full,partial,and deficit irrigation regimes with and without biochar treatment,following a randomized block design.The primary parameters examined were pollen viability(viable,semi-viable,and non-viable rates),anther width and length,and pollen width and length.Microscopic measurements and statistical analyses(p≤0.05)revealed significant effects of both irrigation regimes and biochar applications.Under deficit irrigation,viable,semi-viable,and non-viable pollen rates were 29.84%,32.95%,and 37.21%,respectively,whereas the highest viable pollen rate was observed under full irrigation.In partial irrigation,viable pollen accounted for 31.67%,semi-viable for 38.81%,and non-viable for 29.49%.In plots treated with biochar under partial irrigation,anther width(1700.89μm),anther length(3805.34μm),pollen width(26.93μm),and pollen length(37.42μm)reached the highest values,while the lowest values were recorded in deficit irrigation plots without biochar.These findings emphasize the importance of integrating biochar into irrigation management to mitigate the adverse effects of water stress on pollen development.Nevertheless,further research is needed to clarify the long-term implications of these practices for reproductive success and agricultural sustainability.
基金funded by the Natural Science Foundation of China(Grant No.U22A20600)。
文摘Existing creep constitutive models rarely incorporate studies on the coupling mechanism between creep damage and rock strain softening/hardening.This study analyzed the strain softening and hardening behaviors of argillaceous sandstone and sandy mudstone during load-induced failure based on the plastic increment theory.These behaviors were then coupled with an improved Burgers creep model to establish a coupled creep-damage and plastic softening/hardening model.Finally,the validity and engineering applicability of the proposed model were verified through FLAC~(3D)numerical simulations.The numerical simulation results of standard cylindrical specimens show that the established coupling model can effectively reflect the unloading creep deformation law and failure characteristics of argillaceous sandstone and sandy mudstone.Taking the diversion tunnel of a hydropower station in Northwest China as an example for engineering application,the coupled creep-damage and plastic softening/hardening model is introduced into FLAC~(3D)to carry out numerical simulation calculation of the tunnel under excavation and unsupported creep conditions.The results show that the uncoordinated deformation of the upper and lower walls of the surrounding rock of the tunnel is more prominent.When the buried depth of the tunnel increases to 80 m,the monitoring point C in the sandy mudstone area of the upper wall shows nonlinear accelerated deformation under unsupported creep conditions,and the maximum displacement in the horizontal direction reaches 44.5 mm,and the maximum displacement in the vertical direction reaches 53.5 mm.The coupled creep-damage and plastic softening/hardening model established in the research results can well describe the whole process of uncoordinated deformation and failure in the unloading creep process of soft-hard interbedded rock mass.
基金financed by the National Council for Scientific and Technological Development(CNPq)-Universal Number 18/2021:405731/2021-3.
文摘Affecting about 241.4 million people worldwide,Schistosoma(S.)mansoni,the main etiological agent of intestinal schistosomiasis,is one of the most prevalent diseases in the world remaining a public health issue,especially in developing countries in South America,Africa and the Caribbean.In Brazil,more than 30 million people are at risk of infection,and nearly 1.5 million are infected by S.mansoni[1].Although the implemented control measures proved to be efficient and contributed to a decrease in morbidity and mortality associated with S.mansoni infections,they led to a new epidemiological scenario characterized by low-intensity infections and areas with low prevalence,presenting a new challenge towards the elimination and control of schistosomiasis[2].
基金supported by the National Natural Science Foundation of China(Grant Nos.42307189 and 42030701)the China Postdoctoral Science Foundation(Grant No.2023M740974).
文摘The actively heated fiber-optic(AHFO)technology has emerged as a frontier and hotspot in soil water content measurement,offering advantages such as easy installation,large-scale distributed measurement capability,and resistance to electromagnetic interference.However,current AHFO water content sensors fail to simultaneously achieve high precision,applicability for deep soil,and automated real-time monitoring,thereby limiting their development and application.Therefore,this study introduces a novel actively heated fiber Bragg grating(AH-FBG)cable.Laboratory tests were conducted to assess the heating uniformity of the AH-FBG cable and to establish the temperature characteristic value(T_(t))-soil water content(θ)calibration formula for water content measurement.Subsequently,AH-FBG cables were deployed for in situ soil water content monitoring in a test pit on the Loess Plateau.Through two-year monitoring data verified the accuracy of the AH-FBG cable and elucidated the spatiotemporal distribution of in situ loess water content.Laboratory results demonstrated superior heating uniformity of AHFBG cable,with a T_(t) standard deviation of approximately 0.3℃.In the field,the AH-FBG cable exhibited excellent performance in soil water content measurement,achieving a high accuracy of 0.023 cm^(3)/cm^(3).Further analysis revealed that the θ fluctuation predominantly occurred within a 10 m depth from the soil surface,with an overall upward trend over the two-year monitoring period;the response of shallow θ to precipitation was significant but exhibited increasing hysteresis with depth;frequent precipitation significantly enhanced water infiltration depth.This study provides technical guidance for highprecision,quasi-distributed,automated and real-time water content measurement of deep soil.
基金Supported by the China National Key R&D Program(2023YFF0614100)National Science and Technology Major Project of China(2024ZD14066)+1 种基金Major Project of PetroChina Company Limited(2021ZZ01)Key R&D Project of Xinjiang Uygur Autonomous Region of China(2024B03001).
文摘Based on the technological demands for significantly enhancing oil recovery and long-term CO_(2)sequestration in the lacustrine oil reservoirs of China,this study systematically reviews the progress and practices of CO_(2)flooding and storage technologies in recent years.It addresses the key technological needs and challenges faced in scaling up the application of CO_(2)flooding and storage to mature,developed oil fields,and analyzes future development directions.During the pilot test phase(2006-2019),continuous development and application practices led to the establishment of the first-generation CO_(2)flooding and storage technology system for lacustrine reservoirs.In the industrialization phase(since 2020),significant advances and insights have been achieved in terms of confined phase behavior,storage mechanisms,reservoir engineering,sweep control,engineering process and storage monitoring,enabling the maturation of the second-generation CO_(2)flooding and storage theories and technologies to effectively support the demonstration projects of Carbon Capture,Utilization and Storage(CCUS).To overcome key technical issues such as low miscibility,difficulty in gas channeling control,high process requirements,limited application scenarios,and coordination challenges in CO_(2)flooding and storage,and to support the large-scale application of CCUS,it is necessary to strengthen research on key technologies for establishing the third-generation CO_(2)flooding and storage technological system incorporating miscibility enhancement and transformation,comprehensive regulation for sweep enhancement,whole-process engineering techniques and equipment,long-term storage monitoring safety,and synergistic optimization of flooding and storage.
基金supported by the Natural Science Foundation of Guangdong Province(No.2020A1515110526)the Key-Area Research and Development Programof Guangdong Province(No.2020B1111360003)the National Natural Science Foundation of China(No.42305096).
文摘Nitrogen oxides(NO_(x))are crucial in tropospheric photochemical ozone(O_(3))production and oxidation capacity.Currently,the widely used NO_(x)measurement technique is chemiluminescence(CL)(CL-NO_(x)),which tends to overestimate NO_(2)due to atmospheric oxidation products of NO_(x)(i.e.,NO_(z)).We developed and characterized a NO_(x)measurement system using the cavity attenuated phase shift(CAPS)technique(CAPS-NO_(x)),which is free from interferences with nitrogen-containing species.The NO_(x)measured by the CAPS-NO_(x)and CL-NO_(x)analyzers were compared.Results show that both analyzers showed consistent measurement results for NO,but the NO_(2)measured by the CAPS-NO_(x)analyzer(NO_(2)_CAPS)was mostly lower than that measured by the CL-NO_(x)analyzer(NO_(2)_CL),which led to the deviations in O_(3)formation sensitivity regime and O_(x)(=O_(3)+NO_(2))sources(i.e.,regional background and photochemically produced O_(x))determined by the ozone production efficiencies(OPE)calculated from NO_(2)_CL and NO_(2)_CAPS.Overall,OPE_CL exceeded OPE_CAPS by 18.9%,which shifted 3 out of 13 observation days from the VOCs-limited to the transition regime when judging using OPE_CL,as compared to calculations using OPE_CAPS.During the observation period,days dominated by regional background O_(x)accounted for 46%and 62%when determined using NO_(2)_CL and NO_(2)_CAPS,respectively.These findings suggest that the use of the CL-NO_(x)analyzer tends to underestimate both the VOCs-limited regime and the regional background O_(x)dominated days.The newly built CAPS-NO_(x)analyzer here can promote the accurate measurement of NO_(2),which is meaningful for diagnosing O_(3)formation regimes and O_(x)sources.
文摘α-Trifluoromethyl ketones are a class of useful compounds with versatile applications.Their synthetic application via the transformation of the C—F bonds is of particular interest by allowing the synthesis of organic compounds with diverse structures.Herein,the advances in the research areas ofα-trifluoromethyl ketone synthesis and their defluorination reactions are reviewed.Discussion on the mechanisms of the typical reactions has also been provided,in hope of affording some guides to the chemistry ofα-trifluoromethyl ketones in the synthetic methods toward themselves and their derivatives.
基金supported by the National Key R&D Program of China,No.2019YFA0110300(to ZG)the National Natural Science Foundation of China,Nos.81773302(to YF),32070862(to ZG).
文摘Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.
基金supported by the National Natural Science Foundation of China(52074045,52274074)the Science Fund for Distinguished Young Scholars of Chongqing(CSTB2022NSCQ-JQX0028).
文摘Developing deep fragmented soft coalbed methane(CBM)can significantly enhance domestic natural gas supplies,reduce reliance on imported energy,and bolster national energy security.This manuscript provides a comprehensive review of commonly employed coalbed methane extraction technologies.It then delves into several critical issues in the current stage of CBM exploration and development in China,including the compatibility of existing technologies with CBM reservoirs,the characteristics and occurrence states of CBM reservoirs,critical desorption pressure,and gas generation mechanisms.Our research indicates that current CBM exploration and development technologies in China have reached an internationally advanced level,yet the industry is facing unprecedented challenges.Despite progress in low-permeability,high-value coal seams,significant breakthroughs have not been achieved in exploring other types of coal seams.For different coal reservoirs,integrated extraction technologies have been developed,such as surface pre-depressurisation and segmented hydraulic fracturing of coal seam roof strata.Additionally,techniques like large-scale volume fracturing in horizontal wells have been established,significantly enhancing reservoir stimulation effects and coalbed methane recovery rates.However,all of these technologies are fundamentally based on permeation.These technologies lack direct methods aimed at enhancing the diffusion rate of CBM,thereby failing to fully reflect the unique characteristics of CBM.Current CBM exploration and development theories and technologies are not universally applicable to all coal seams.They do not adequately account for the predominantly adsorbed state of CBM,and the complex and variable gas generation mechanisms further constrain CBM development in China.Finally,continuous exploration of new deep CBM exploration technologies is necessary.Integrating more effective reservoir stimulation technologies is essential to enhance technical adaptability concerning CBM reservoir characteristics,gas occurrence states,and gas generation mechanisms,ultimately achieving efficient CBM development.We conclude that while China possesses a substantial foundation of deep fractured CBM resources,industry development is constrained and requires continuous exploration of new CBM exploration and development technologies to utilize these resources effectively.
基金funded by the National Natural Science Foundation of China(No.41962016)the Natural Science Foundation of NingXia(Nos.2023AAC02023,2023A1218,and 2021AAC02006).
文摘Soil improvement is one of the most important issues in geotechnical engineering practice.The wide application of traditional improvement techniques(cement/chemical materials)are limited due to damage ecological en-vironment and intensify carbon emissions.However,the use of microbially induced calcium carbonate pre-cipitation(MICP)to obtain bio-cement is a novel technique with the potential to induce soil stability,providing a low-carbon,environment-friendly,and sustainable integrated solution for some geotechnical engineering pro-blems in the environment.This paper presents a comprehensive review of the latest progress in soil improvement based on the MICP strategy.It systematically summarizes and overviews the mineralization mechanism,influ-encing factors,improved methods,engineering characteristics,and current field application status of the MICP.Additionally,it also explores the limitations and correspondingly proposes prospective applications via the MICP approach for soil improvement.This review indicates that the utilization of different environmental calcium-based wastes in MICP and combination of materials and MICP are conducive to meeting engineering and market demand.Furthermore,we recommend and encourage global collaborative study and practice with a view to commercializing MICP technique in the future.The current review purports to provide insights for engineers and interdisciplinary researchers,and guidance for future engineering applications.
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
文摘The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established by the World Health Organization(WHO). Reform in China has demonstrated the unwavering confidence and utmost determination of the Chinese government and the Chinese transplantation community. The year 2015 marked a historic turning point when voluntary donations from Chinese citizens became the sole legitimate source for organ transplantation. Since 2015, China has gradually established and refined the “Chinese Mode” and “China System” for organ donation and transplantation, fulfilling its political pledge of reform, and has garnered international recognition, and fostered a social culture which promotes organ donation. This article reviewed the history of reform on organ donation and transplantation in China, presented a new pattern of establishment of organ donation system in the new era of the country, and the direction of advances in the future.
基金supported by the GRDC(Global Research Development Center)Cooperative Hub Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(MSIT)(No.RS-2023-00257595).
文摘Liquid metals(LMs),because of their ability to remain in a liquid state at room temperature,render them highly versatile for applications in electronics,energy storage,medicine,and robotics.Among various LMs,Ga-based LMs exhibit minimal cytotoxicity,low viscosity,high thermal and electrical conductivities,and excellent wettability.Therefore,Ga-based LM composites(LMCs)have emerged as a recent research focus.Recent advancements have focused on novel fabrication techniques and applications spanning energy storage,flexible electronics,and biomedical devices.Particularly noteworthy are the developments in wearable sensors and electronic skins,which hold promise for healthcare monitoring and human-machine interfaces.Despite their potential,challenges,such as oxidative susceptibil-ity and biocompatibility,remain.Creating bio-based LMC materials is a promising approach to address these issues while exploring new avenues to optimize LMC performance and broaden its application domains.This review provides a concise overview of the recent trends in LMC research,highlights their transformative impacts,and outlines key directions for future investigation and development.
