Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versat...Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versatile method was developed to fabricate flexible conjugated microporous polymer films for fast detecting the total quantity of developing agents and their oxidation products.Conjugated microporous polymer films were synthesized through in situ one-step approach by Suzuki coupling reaction.With such merits such as intense luminescence,easy synthesis and disposability,the polymer films were employed to detect developers based on fluorescence quenching in the printing wastewater.A linear response to p-benzoquinone,oxidation product of developers,was obtained in the range of 0.05-0.5 lmolL-1,with a detection limit of 0.015 lmolL-1.The strategy for in situ growth of flexible polyphenylene-conjugated microporous polymer(PP-CMP)films seems to be a very portable and general method for meeting different analytic purposes.展开更多
With the rapid advance of academic activities in the field of phar-macology,antiinflammatory and immunopharmacology has become abranch of pharmacology in our country.Under professor Zhou Jin-huang’s charge and concer...With the rapid advance of academic activities in the field of phar-macology,antiinflammatory and immunopharmacology has become abranch of pharmacology in our country.Under professor Zhou Jin-huang’s charge and concerns,the first national conference of anti-inflammatory and immunopharmacology was held at Huang Shan in1982.In following years,the frist training class for antiinflamma-tory and immunopharmacological studies was conducted in Hefei.展开更多
This review starts with a brief history of drug discovery & development, and the place of Asia in this worldwide effort discussed. The conditions and constraints of a successful translational R&D involving aca...This review starts with a brief history of drug discovery & development, and the place of Asia in this worldwide effort discussed. The conditions and constraints of a successful translational R&D involving academic basic research and clinical research are discussed and the Singapore model for pursuit of open R&D described. The importance of well-characterized, validated drug targets for the search for novel targeted anti-cancer agents is emphasized, as well as a structured, high quality translational R&D. Furthermore, the characteristics of an attractive preclinical development drug candidate are discussed laying the foundation of a successful preclinical development. The most frequent sources of failures are described and risk management at every stage is highly recommended. Organizational factors are also considered to play an important role. The factors to consider before starting a new drug discovery & development project are described, and an example is given of a successful clinical project that has had its roots in local universities and was carried through preclinical development into phase I clinical trials.展开更多
[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing d...[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.展开更多
Background:Invaluable forensic evidence can be found in latent fingerprints,which are invisible to the naked eye.The integrity of the evidence may be compromised by conventional fingerprint production techniques that ...Background:Invaluable forensic evidence can be found in latent fingerprints,which are invisible to the naked eye.The integrity of the evidence may be compromised by conventional fingerprint production techniques that use chemicals,powders,and lasers and pose environmental dangers.Pomegranate peel powder,made from the rind of the fruit that is native to the Middle East and SouthAsia,offers a natural alternative.Aims and Objectives:The usefulness of pomegranate peel powder as a non-damaging agent for latent fingerprint development on a variety of non-porous surfaces is investigated in this study.Materials and Methods:The powder is used to develop all three types of fingerprints i.e.sebaceous,eccrine and natural on various non-porous surfaces(steel,glass slide,iron and granite).Results:According to our study,pomegranate peel powder produces remarkable outcomes and highlights its non-toxic properties and ability to preserve the evidence.Conclusion:Pomegranate peel powder can be an effective and environmentally friendly alternative to traditional techniques for fingerprinting in forensic science.展开更多
The integration of Large Language Models(LLMs)into mobile and software development workflows faces a persistent tension among three demands:semantic awareness,developer productivity,and data privacy.Traditional cloud-...The integration of Large Language Models(LLMs)into mobile and software development workflows faces a persistent tension among three demands:semantic awareness,developer productivity,and data privacy.Traditional cloud-based tools offer strong reasoning but risk data exposure and latency,while on-device solutions lack full-context understanding across codebase and developer tooling.We introduce SolidGPT,an open-source,edge-cloud hybrid developer assistant built on GitHub,designed to enhance code and workspace semantic search.SolidGPT enables developers to below.Talk to your codebase:interactively query code and project structure,discovering the right methods and modules without manual searching.Automate software project workflows:generate PRDs,task breakdowns,Kanban boards,and even scaffold web app beginnings,with deep integration via VSCode and Notion.Configure private,extensible agents:onboard private code folders(up to~500 files),connect Notion,customize AI agent personas via embedding and in-context training,and deploy via Docker,CLI,or VSCode extension.In practice,SolidGPT empowers developer productivity through.Semantic-rich code navigation:no more hunting through files-or wondering where a feature lives.Integrated documentation and task management:seamlessly sync generated PRD content and task boards into developer workflows.Privacy-first design:running locally via Docker or VSCode,with full control over code and data,while optionally reaching out to LLM APIs as needed.By combining interactive code querying,automated project scaffolding,and human-AI collaboration,SolidGPT provides a practical,privacy-respecting edge assistant that accelerates real-world development workflows-ideal for intelligent mobile and software engineering contexts.展开更多
Coronaviruses have repeatedly emerged in recent years,causing significant and ongoing threats to global public health.The development of therapeutic agents and strategies capable of responding to future outbreaks caus...Coronaviruses have repeatedly emerged in recent years,causing significant and ongoing threats to global public health.The development of therapeutic agents and strategies capable of responding to future outbreaks caused by emerging coronavirus variants remain an ongoing priority.Here,we engineered a single-stranded DNA aptamer(NApt8-3)that selectively binds to the conserved nucleocapsid(N)protein shared among multiple coronaviruses,including SARS-CoV-2(wild-type,beta,omicron variant),SARS-CoV,MERS-CoV,HCoV-OC43 and HCoV-229E,and strongly inhibits N protein-induced inflammatory cytokine expression.Mechanistically,NApt8-3 effectively binds to the N protein and blocks its interaction with the NLRP3 inflammasome,a key mediator of coronavirus-induced inflammation.To enable intracellular delivery and evaluate its therapeutic potential,we developed a proof-of-concept anti-SARS-CoV-2 agent—circSASON,a circular trivalent aptamer-antisense oligonucleotide(ASO)chimera—combining NApt8-3,an antispike protein aptamer,and an ASO that silences the N gene.In vitro experiments demonstrated that circSASON effectively inhibits SARS-CoV-2 replication and suppresses N protein-induced cytokine expression in host cells.The intranasal administration of circSASON significantly decreased the level of SARS-CoV-2 and alleviated SARS-CoV-2-induced pulmonary inflammation and inflammatory cytokine expression in mice.Therefore,our findings highlight NApt8-3 as a broad-spectrum anti-inflammatory agent that targets the conserved coronavirus N protein.The therapeutic design strategy employed,together with the N aptamer developed in this study,may offer a framework for the rapid development of treatments to combat future pandemics caused by emerging coronavirus variants.展开更多
Forensic science plays a crucial role in criminal investigation by providing scientific evidence from crime scenes,and fingerprints remain is most encountered form of physical evidence.Fingerprints,classified as paten...Forensic science plays a crucial role in criminal investigation by providing scientific evidence from crime scenes,and fingerprints remain is most encountered form of physical evidence.Fingerprints,classified as patent,plastic,or latent print,are unique to individuals and can aid in personal identification.Latent fingerprints,which are often invisible to the naked eye,require specialized development techniques to be visualized.Traditional methods such as powder methods and chemical methods including iodine fuming,silver nitrate,and cyanoacrylate fuming have been useful for the identification of fingerprints,but limitations arise with older or environmentally exposed prints.In recent years,nanotechnology has been recognized as a powerful tool in forensic science for the development of invisible and latent fingerprints.This review explores the use of nanoparticles,such as metal oxide,in enhancing fingerprint detection.Nanoparticles offer increased sensitivity,selectivity,and the ability to visualize prints on complex surfaces.The application of nanomaterials in latent fingerprint analysis provides new possibilities for criminal identification,paving the way for more accurate and efficient forensic investigations.展开更多
The aerial oxidation kinetics of hydroquinone in a freshly prepared developer solution at different temperatures and pHs has been studied. The activation parameters, Ea, ΔG#, ΔS#, ΔH# and enthalpy of formation of a...The aerial oxidation kinetics of hydroquinone in a freshly prepared developer solution at different temperatures and pHs has been studied. The activation parameters, Ea, ΔG#, ΔS#, ΔH# and enthalpy of formation of activated complex, ΔH0f(X# ), are determined. The large negative value of free energy of activation ΔG# proves that hydroquinone extremely tends to be oxidized by air at optimum temperature (20°C and optimum pH (10.5) and converts to the activated complex semiquinone. It was also found that if the pH of the developer solution is increased from 9.3 to 10.5 the reaction rate will increase by a factor of 2.展开更多
基金The work were supported by the National Natural Science Foundation of China(Nos.21575168,21277176,21475153 and 21675178)the Guangdong Provincial Natural Science Foundation of China(No.2015A030311020)+1 种基金the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China(No.2015A030401036)the Guangzhou Science and Technology Program of China(No.201604020165),respectively。
文摘Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versatile method was developed to fabricate flexible conjugated microporous polymer films for fast detecting the total quantity of developing agents and their oxidation products.Conjugated microporous polymer films were synthesized through in situ one-step approach by Suzuki coupling reaction.With such merits such as intense luminescence,easy synthesis and disposability,the polymer films were employed to detect developers based on fluorescence quenching in the printing wastewater.A linear response to p-benzoquinone,oxidation product of developers,was obtained in the range of 0.05-0.5 lmolL-1,with a detection limit of 0.015 lmolL-1.The strategy for in situ growth of flexible polyphenylene-conjugated microporous polymer(PP-CMP)films seems to be a very portable and general method for meeting different analytic purposes.
文摘With the rapid advance of academic activities in the field of phar-macology,antiinflammatory and immunopharmacology has become abranch of pharmacology in our country.Under professor Zhou Jin-huang’s charge and concerns,the first national conference of anti-inflammatory and immunopharmacology was held at Huang Shan in1982.In following years,the frist training class for antiinflamma-tory and immunopharmacological studies was conducted in Hefei.
文摘This review starts with a brief history of drug discovery & development, and the place of Asia in this worldwide effort discussed. The conditions and constraints of a successful translational R&D involving academic basic research and clinical research are discussed and the Singapore model for pursuit of open R&D described. The importance of well-characterized, validated drug targets for the search for novel targeted anti-cancer agents is emphasized, as well as a structured, high quality translational R&D. Furthermore, the characteristics of an attractive preclinical development drug candidate are discussed laying the foundation of a successful preclinical development. The most frequent sources of failures are described and risk management at every stage is highly recommended. Organizational factors are also considered to play an important role. The factors to consider before starting a new drug discovery & development project are described, and an example is given of a successful clinical project that has had its roots in local universities and was carried through preclinical development into phase I clinical trials.
基金Supported by Innovation Project of Guangxi Graduate Education of GXUCM(YCSY2022012)High-level Innovation Team and Outstanding Scholars Program of Universities and Colleges in Guangxi(GJR[2014]07)Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica(20-065-38).
文摘[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.
文摘Background:Invaluable forensic evidence can be found in latent fingerprints,which are invisible to the naked eye.The integrity of the evidence may be compromised by conventional fingerprint production techniques that use chemicals,powders,and lasers and pose environmental dangers.Pomegranate peel powder,made from the rind of the fruit that is native to the Middle East and SouthAsia,offers a natural alternative.Aims and Objectives:The usefulness of pomegranate peel powder as a non-damaging agent for latent fingerprint development on a variety of non-porous surfaces is investigated in this study.Materials and Methods:The powder is used to develop all three types of fingerprints i.e.sebaceous,eccrine and natural on various non-porous surfaces(steel,glass slide,iron and granite).Results:According to our study,pomegranate peel powder produces remarkable outcomes and highlights its non-toxic properties and ability to preserve the evidence.Conclusion:Pomegranate peel powder can be an effective and environmentally friendly alternative to traditional techniques for fingerprinting in forensic science.
文摘The integration of Large Language Models(LLMs)into mobile and software development workflows faces a persistent tension among three demands:semantic awareness,developer productivity,and data privacy.Traditional cloud-based tools offer strong reasoning but risk data exposure and latency,while on-device solutions lack full-context understanding across codebase and developer tooling.We introduce SolidGPT,an open-source,edge-cloud hybrid developer assistant built on GitHub,designed to enhance code and workspace semantic search.SolidGPT enables developers to below.Talk to your codebase:interactively query code and project structure,discovering the right methods and modules without manual searching.Automate software project workflows:generate PRDs,task breakdowns,Kanban boards,and even scaffold web app beginnings,with deep integration via VSCode and Notion.Configure private,extensible agents:onboard private code folders(up to~500 files),connect Notion,customize AI agent personas via embedding and in-context training,and deploy via Docker,CLI,or VSCode extension.In practice,SolidGPT empowers developer productivity through.Semantic-rich code navigation:no more hunting through files-or wondering where a feature lives.Integrated documentation and task management:seamlessly sync generated PRD content and task boards into developer workflows.Privacy-first design:running locally via Docker or VSCode,with full control over code and data,while optionally reaching out to LLM APIs as needed.By combining interactive code querying,automated project scaffolding,and human-AI collaboration,SolidGPT provides a practical,privacy-respecting edge assistant that accelerates real-world development workflows-ideal for intelligent mobile and software engineering contexts.
基金supported by grants from the National Key R&D Program of China(2021YFF0702000)the National Natural Science Foundation of China(32571620,32170577)+2 种基金the Jilin Province Natural Science Foundation(YDZJ202201ZYTS526,20250101044JJ)the Fundamental Research Funds for the Central Universities(2412024QD020)The authors gratefully acknowledge the assistance of ChatGPT(GPT-5)in refining and improving the clarity of the English writing in this manuscript.
文摘Coronaviruses have repeatedly emerged in recent years,causing significant and ongoing threats to global public health.The development of therapeutic agents and strategies capable of responding to future outbreaks caused by emerging coronavirus variants remain an ongoing priority.Here,we engineered a single-stranded DNA aptamer(NApt8-3)that selectively binds to the conserved nucleocapsid(N)protein shared among multiple coronaviruses,including SARS-CoV-2(wild-type,beta,omicron variant),SARS-CoV,MERS-CoV,HCoV-OC43 and HCoV-229E,and strongly inhibits N protein-induced inflammatory cytokine expression.Mechanistically,NApt8-3 effectively binds to the N protein and blocks its interaction with the NLRP3 inflammasome,a key mediator of coronavirus-induced inflammation.To enable intracellular delivery and evaluate its therapeutic potential,we developed a proof-of-concept anti-SARS-CoV-2 agent—circSASON,a circular trivalent aptamer-antisense oligonucleotide(ASO)chimera—combining NApt8-3,an antispike protein aptamer,and an ASO that silences the N gene.In vitro experiments demonstrated that circSASON effectively inhibits SARS-CoV-2 replication and suppresses N protein-induced cytokine expression in host cells.The intranasal administration of circSASON significantly decreased the level of SARS-CoV-2 and alleviated SARS-CoV-2-induced pulmonary inflammation and inflammatory cytokine expression in mice.Therefore,our findings highlight NApt8-3 as a broad-spectrum anti-inflammatory agent that targets the conserved coronavirus N protein.The therapeutic design strategy employed,together with the N aptamer developed in this study,may offer a framework for the rapid development of treatments to combat future pandemics caused by emerging coronavirus variants.
文摘Forensic science plays a crucial role in criminal investigation by providing scientific evidence from crime scenes,and fingerprints remain is most encountered form of physical evidence.Fingerprints,classified as patent,plastic,or latent print,are unique to individuals and can aid in personal identification.Latent fingerprints,which are often invisible to the naked eye,require specialized development techniques to be visualized.Traditional methods such as powder methods and chemical methods including iodine fuming,silver nitrate,and cyanoacrylate fuming have been useful for the identification of fingerprints,but limitations arise with older or environmentally exposed prints.In recent years,nanotechnology has been recognized as a powerful tool in forensic science for the development of invisible and latent fingerprints.This review explores the use of nanoparticles,such as metal oxide,in enhancing fingerprint detection.Nanoparticles offer increased sensitivity,selectivity,and the ability to visualize prints on complex surfaces.The application of nanomaterials in latent fingerprint analysis provides new possibilities for criminal identification,paving the way for more accurate and efficient forensic investigations.
文摘The aerial oxidation kinetics of hydroquinone in a freshly prepared developer solution at different temperatures and pHs has been studied. The activation parameters, Ea, ΔG#, ΔS#, ΔH# and enthalpy of formation of activated complex, ΔH0f(X# ), are determined. The large negative value of free energy of activation ΔG# proves that hydroquinone extremely tends to be oxidized by air at optimum temperature (20°C and optimum pH (10.5) and converts to the activated complex semiquinone. It was also found that if the pH of the developer solution is increased from 9.3 to 10.5 the reaction rate will increase by a factor of 2.
