Lysozyme possesses unique biological properties and finds wide-ranging applications,but the adaptive responses of microorganisms under lysozyme stress remain poorly understood.In this study,we investigated the respons...Lysozyme possesses unique biological properties and finds wide-ranging applications,but the adaptive responses of microorganisms under lysozyme stress remain poorly understood.In this study,we investigated the response mechanisms of Dermacoccus abyssi HZAU 226 and Kocuria rhizophila H9,which with different lysozyme resistance.The results revealed similar regulatory mechanisms in two strains.D.abyssi demonstrated resistance to cationic activity and displayed typical growth curves,membrane permeability was concentration-dependent.Under 5 mg/mL lysozyme stress,the isoelectric point increased and the negative zeta potential decreased.The hydrophobicity and adhesion with a concomitant increase in the surface N/C and P/C element ratios,cells stretched in length and increased adhesion to limit lysozyme diffusion,and peptidoglycan modification and two-component regulatory genes were significantly up-regulated.The findings help to understand the relationship between adaptive stress responses and lysozyme resistance,provided a theoretical basis for the development of new food antibacterial agents.展开更多
基金supported by grants from the Major Science and Technology Fund of Guangxi Province(AB23026035)the Hubei Province Health and Family Planning Scientific Research Project(No.WJ 2023M168).
文摘Lysozyme possesses unique biological properties and finds wide-ranging applications,but the adaptive responses of microorganisms under lysozyme stress remain poorly understood.In this study,we investigated the response mechanisms of Dermacoccus abyssi HZAU 226 and Kocuria rhizophila H9,which with different lysozyme resistance.The results revealed similar regulatory mechanisms in two strains.D.abyssi demonstrated resistance to cationic activity and displayed typical growth curves,membrane permeability was concentration-dependent.Under 5 mg/mL lysozyme stress,the isoelectric point increased and the negative zeta potential decreased.The hydrophobicity and adhesion with a concomitant increase in the surface N/C and P/C element ratios,cells stretched in length and increased adhesion to limit lysozyme diffusion,and peptidoglycan modification and two-component regulatory genes were significantly up-regulated.The findings help to understand the relationship between adaptive stress responses and lysozyme resistance,provided a theoretical basis for the development of new food antibacterial agents.
