The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for derepli...The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for dereplication from complex mixture.To solve the inefficient discovery process of natural DAs,a novel dereplication strategy based on pattern recognition of specific heteronuclear multiple bond correlation(HMBC)signals has been established in this study.This strategy utilized the diagnostic signals in the HMBC spectra of C_(19)-DAs,which could indicate the structural categories.The newly designed software MatchNat allowed in silico recognition of C_(19)-DA types.The developed dereplication system was demonstrated to be a practical strategy to dereplicate a variety of natural products from two Delphineae plants,including Aconitum vilmorinianum Kom.and Delphinium pachycentrum Hemsl.Known compounds could be rapidly recognized from the mixture,and novel DAs were found with the aid of MatchNat.Consequently,targeted isolation led to the explosive discovery of a series of novel DAs.This dereplication system has the potential to be adopted for dereplication of any other class of natural products and has shown promising application as a complementary tool in the nuclear magnetic resonance analysis of traditional Chinese medicines.展开更多
Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The ...Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The search for novel natural products requires a quick and efficient approach to distinguish novel compounds from the known ones,a process called dereplication.Dereplication strategy is generic and time-saving,avoids isolation/purification of known compounds,enables an efficient liquid chromatography peak annotation of most of the studied compounds and can be well adapted for plant chemotaxonomy,phytochemical screening and metabolite profiling.In this review,recent developments of hyphenated techniques towards dereplication of active compounds,chemotaxonomy,metabolite profiling and rapid detection of novel compounds in medicinal plant extracts are presented.展开更多
Aims: Twelve crude extracts were prepared from different parts of Pluchea sagittalis, Cecropia palmata and Brachiaria brizantha, and their effect on the light reaction of photosynthesis. Study Design: Use of fluoresce...Aims: Twelve crude extracts were prepared from different parts of Pluchea sagittalis, Cecropia palmata and Brachiaria brizantha, and their effect on the light reaction of photosynthesis. Study Design: Use of fluorescence of ChL a (Chlorophyll a) to detect inhibitors of photosynthesis in plant extracts. Place and Duration of Study: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Ciências Exatas e da Terra, Universidade Federal de Sao Paulo (UNIFESP), between July 2015 and January 2016. Methodology: The samples had their effect on the light reaction of photosynthesis studied by Chlorophyll a (Chl a) fluorescence transient by the use of chloroplasts isolated from spinach leaves. As negative controls we used the same amounts of DMSO that have been employed to solubilize the samples, and as positive control we have used 10 μM of the commercial herbicide DCMU (Diuron). Results: The extracts inhibited the photosynthesis, and their inhibition sites were located in the range of electron flow from OEC complex and between P<sub>680</sub> to Q<sub>A</sub> of PS II, and inhibited the photosystem II (PS II) by inducing the appearance of the transient bands K and J. Conclusion: Our results suggest an indicative that the photochemical apparatus probably is failing at the donor and acceptor sides of PSII, causing damage to electron transport during the photosynthesis phenomenon.展开更多
基金funded by the Science and Technology Development Fund,Macao SAR(File Nos.0023/2019/AKP,0077/2019/A2,0002/2019/APD,and 0040/2021/AGJ)the National Natural Science Foundation of China(No.31860095)。
文摘The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for dereplication from complex mixture.To solve the inefficient discovery process of natural DAs,a novel dereplication strategy based on pattern recognition of specific heteronuclear multiple bond correlation(HMBC)signals has been established in this study.This strategy utilized the diagnostic signals in the HMBC spectra of C_(19)-DAs,which could indicate the structural categories.The newly designed software MatchNat allowed in silico recognition of C_(19)-DA types.The developed dereplication system was demonstrated to be a practical strategy to dereplicate a variety of natural products from two Delphineae plants,including Aconitum vilmorinianum Kom.and Delphinium pachycentrum Hemsl.Known compounds could be rapidly recognized from the mixture,and novel DAs were found with the aid of MatchNat.Consequently,targeted isolation led to the explosive discovery of a series of novel DAs.This dereplication system has the potential to be adopted for dereplication of any other class of natural products and has shown promising application as a complementary tool in the nuclear magnetic resonance analysis of traditional Chinese medicines.
文摘Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The search for novel natural products requires a quick and efficient approach to distinguish novel compounds from the known ones,a process called dereplication.Dereplication strategy is generic and time-saving,avoids isolation/purification of known compounds,enables an efficient liquid chromatography peak annotation of most of the studied compounds and can be well adapted for plant chemotaxonomy,phytochemical screening and metabolite profiling.In this review,recent developments of hyphenated techniques towards dereplication of active compounds,chemotaxonomy,metabolite profiling and rapid detection of novel compounds in medicinal plant extracts are presented.
文摘Aims: Twelve crude extracts were prepared from different parts of Pluchea sagittalis, Cecropia palmata and Brachiaria brizantha, and their effect on the light reaction of photosynthesis. Study Design: Use of fluorescence of ChL a (Chlorophyll a) to detect inhibitors of photosynthesis in plant extracts. Place and Duration of Study: Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Ciências Exatas e da Terra, Universidade Federal de Sao Paulo (UNIFESP), between July 2015 and January 2016. Methodology: The samples had their effect on the light reaction of photosynthesis studied by Chlorophyll a (Chl a) fluorescence transient by the use of chloroplasts isolated from spinach leaves. As negative controls we used the same amounts of DMSO that have been employed to solubilize the samples, and as positive control we have used 10 μM of the commercial herbicide DCMU (Diuron). Results: The extracts inhibited the photosynthesis, and their inhibition sites were located in the range of electron flow from OEC complex and between P<sub>680</sub> to Q<sub>A</sub> of PS II, and inhibited the photosystem II (PS II) by inducing the appearance of the transient bands K and J. Conclusion: Our results suggest an indicative that the photochemical apparatus probably is failing at the donor and acceptor sides of PSII, causing damage to electron transport during the photosynthesis phenomenon.
