Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n...Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n situ pistil delivery （ISPDy method with no tissue culture and adaptive to three Brassica species. Briefly this method can be stated as placement of a liquid drop containing binary vector DNA onto the cut surface of emasculated and artificial pollinated Brassica stigma. Transformation rate （Basta-resistant seedlings per 100 pistils） of different species ranged from 0.4% to 16.3%. Factors affecting transforma-tion success included tinning and buffer type. PCR and Southern blot confirmed transgenic events. Reciprocal cross showed reporter gene steadily inherited in nuclear transformed pattern. GUS histochemical assay of transgenic immature seeds showed stained embroys and endosperm in the same seed, indicating DNA delivered through style and integrated into Brassica seed/genome after double fertilization.展开更多
Objective: This study focuses on the clinical observation of the impact of different treatment methods for gestational heart failure on delivery outcomes. Method: A total of 160 pregnant women with heart failure admit...Objective: This study focuses on the clinical observation of the impact of different treatment methods for gestational heart failure on delivery outcomes. Method: A total of 160 pregnant women with heart failure admitted to our hospital between October 2020 and October 2021 were selected as the study subjects. They were categorized based on delivery mode, delivery timing, heart failure control time, and cardiac function status. The delivery outcomes of the different groups were then compared. Result: In terms of delivery methods, the rate of neonatal asphyxia was higher following vaginal delivery than cesarean section. Regarding delivery timing, the neonatal mortality rate was lower for cesarean sections performed at 32-36 + 6 weeks compared to those conducted at 37-39 + 6 weeks. With respect to heart failure control time, the rates of neonatal asphyxia and pulmonary hyaline membrane disease were lower in the ≤ 48- hour group than in the > 48-hour group. From the perspective of cardiac function status, patients with cardiac function I- II exhibited relatively lower rates of neonatal asphyxia and perinatal mortality compared to those with cardiac function III-IV. The observed differences were statistically significant (P < 0.05). Conclusion: For patients with gestational heart failure, cesarean section is the recommended mode of delivery, with the optimal timing being between 32 and 36+6 weeks of pregnancy. During cesarean section, the timing of delivery should be carefully selected based on the mother’s cardiac function status.展开更多
RNAi trigged by dsRNA not only facilitates the development of molecular biology, but also initiates a new way for pest control by silence of fatal genes. However, one of the key limitations in pest control is lack of ...RNAi trigged by dsRNA not only facilitates the development of molecular biology, but also initiates a new way for pest control by silence of fatal genes. However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one- time injection of larvae with dsRNA could reduce the pupation rate by 43.0% and enhance larva mortality by 11.7%. One- time ingestion of dsRNA did not result in any significant effect on phenotype. Continuous ingestion of in vitro synthesized dsRNA by refreshing the bait diet every day caused 40.4% decrease in successful pupation and 10.0% increase in larval mortality, which was similar as one-time injection. The most efficient method was found to be the continuous ingestion of the bacteria containing dsRNA expressed, which reduced the rate of pupation by 68.7% and enhanced the larval mortality by 34.1%. Further analysis found that dsRNA was degraded faster in midgut juice than in hemolymph. However, the cell of bacteria could protect dsRNA and delay the degradation in the midgut juice of H. armigera. These results throw light on the application of dsRNA in pest management with proper ways.展开更多
The selection of the project delivery method(PDM)for any project is critical—it establishes the communication,coordination,and contractual interfaces between the owner,contractor,and designer.With an increase in the ...The selection of the project delivery method(PDM)for any project is critical—it establishes the communication,coordination,and contractual interfaces between the owner,contractor,and designer.With an increase in the number of green design projects,understanding the relationship between the PDM and green design is paramount to project and contract management.While it is reasonable to assume that a relationship between green design and design-build(DB)exists since both theoretically are intended to foster an integrated,holistic,team-based collaborative project;it is also appropriate to understand the relationship between green design and other PDMs.This preliminary research examined possible relationships between design-bid-build(DBB),construction management(CM),and DB PDMs and green design with the goal of beginning to identify potential positive correspondence between them.To develop an initial understanding,two main tasks were completed.First,existing published research was evaluated to reveal aspects of projects and PDMs independent of green design.Second,the research collected primarily qualitative information by conducting structured interviews with approximately twenty-five individuals,including owners,contractors,and designers involved in completed green design projects,mainly in the public sector.Upon completion of the structured interviews,responses from the interviews were reviewed for green design project characteristics.Additionally,the interviews provided insight on the initial understanding of the current state of knowledge and experience in green design and PDMs.After the two main tasks were completed,both were evaluated for commonalities.Preliminary results found that seven green design project characteristics emerged that in some cases related to a specific PDM,but in other cases were independent of a PDM.Future research will quantitatively investigate the relationship between green design projects,PDMs,and success factors.展开更多
Diabetic patients often suffer higher orthopedic implant failure rates due to high blood sugar causing inflammation and oxidative stress.While molecular hydrogen(H_(2))shows anti-inflammatory and antioxidant promise,c...Diabetic patients often suffer higher orthopedic implant failure rates due to high blood sugar causing inflammation and oxidative stress.While molecular hydrogen(H_(2))shows anti-inflammatory and antioxidant promise,current delivery methods lack precision for sustained implantsite treatment.A collaborative team led by Prof.展开更多
Spinal cord injuries have overwhelming physical and occupational implications for patients.Moreover,the extensive and long-term medical care required for spinal cord injury significantly increases healthcare costs and...Spinal cord injuries have overwhelming physical and occupational implications for patients.Moreover,the extensive and long-term medical care required for spinal cord injury significantly increases healthcare costs and resources,adding a substantial burden to the healthcare system and patients'families.In this context,chondroitinase ABC,a bacterial enzyme isolated from Proteus vulgaris that is modified to facilitate expression and secretion in mammals,has emerged as a promising therapeutic agent.It works by degrading chondroitin sulfate proteoglycans,cleaving the glycosaminoglycanchains of chondroitin sulfate proteoglycans into soluble disaccharides or tetrasaccharides.Chondroitin sulfate proteoglycans are potent axon growth inhibitors and principal constituents of the extracellular matrix surrounding glial and neuronal cells attached to glycosaminoglycan chains.Chondroitinase ABC has been shown to play an effective role in promoting recovery from acute and chronic spinal cord injury by improving axonal regeneration and sprouting,enhancing the plasticity of perineuronal nets,inhibiting neuronal apoptosis,and modulating immune responses in various animal models.In this review,we introduce the classification and pathological mechanisms of spinal cord injury and discuss the pathophysiological role of chondroitin sulfate proteoglycans in spinal cord injury.We also highlight research advancements in spinal cord injury treatment strategies,with a focus on chondroitinase ABC,and illustrate how improvements in chondroitinase ABC stability,enzymatic activity,and delivery methods have enhanced injured spinal cord repair.Furthermore,we emphasize that combination treatment with chondroitinase ABC further enhances therapeutic efficacy.This review aimed to provide a comprehensive understanding of the current trends and future directions of chondroitinase ABC-based spinal cord injury therapies,with an emphasis on how modern technologies are accelerating the optimization of chondroitinase ABC development.展开更多
BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores ...BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores the potential of retroauricular subperiosteal injection as a localized drug delivery method for a more effective and safe treatment.AIM To compare the efficacy of retroauricular subperiosteal injection vs systemic intravenous glucocorticoid(GC)administration for SD in patients with DM and assess the effects on blood glucose levels.METHODS A total of 128 cases of type 2 DM(T2DM)with SD diagnosed and treated in Zibo Central Hospital from February 2021 to July 2023 were divided into two groups:An observation group(66 cases receiving retroauricular subperiosteal injection of methylprednisolone)and a control group(62 cases receiving systemic intravenous administration of methylprednisolone).The two groups were compared in terms of therapeutic efficacy,hearing recovery,blood glucose level changes,and incidence of adverse reactions.Binary logistic regression was used to analyze the factors affecting therapeutic efficacy.RESULTS The observation group showed a significantly higher total effective rate(90.91%)compared with the control group(75.81%,P<0.05).Additionally,pure-tone hearing threshold,fasting plasma glucose,and 2-hour postprandial blood glucose were significantly lower in the observation group compared with the control group(P<0.05).The incidence of adverse reactions was also lower in the observation group than in the control group(7.58%vs 22.58%,P<0.05).A T2DM course longer than 5 years and systemic intravenous GC administration were identified as independent risk factors for treatment inefficacy(P<0.05).INTRODUCTION Sudden deafness(SD)is a clinical emergency characterized by rapid-onset hearing loss that is often accompanied by clinical symptoms such as tinnitus and vertigo[1].Although its pathogenesis remains unclear,it is supposedly associated with factors,including inner ear microcirculation disorders,autoimmune diseases,and viral infections[2,3].Patients with diabetes are particularly susceptible to microvascular complications due to poor long-term glycemic control,affecting the ear’s microcirculation,subsequently increasing the risk of SD[4].Type 2 diabetes mellitus(T2DM),a chronic metabolic disease,causes multiple microvascular damages throughout the body,complicating SD treatment in patients with diabetes[5,6].Inner ear microcirculation disturbances in patients with diabetes may exacerbate the risk of SD,and its pathological process may be related to vascular endothelial dysfunction,inflammatory reactions,and hemorrhological changes caused by diabetes mellitus(DM)[7].Current SD treatments include glucocorticoids(GCs),vasodilators,and hyperbaric oxygen therapy[8].GCs are widely used due to their anti-inflammatory and immunosuppressive effects[9].However,systemic GCs may cause blood glucose(BG)fluctuations and even increase the risk of complications in patients with DM,limiting their clinical use in this population[10].Therefore,there is a compelling and immediate need for a local alternative solution that minimally affects the metabolic mechanisms.In recent years,retroauricular subperiosteal injection has emerged as a localized administration modality for treating SD[11].This method allows drugs to directly act on the inner ear,avoiding the side effects of systemic administration and having a minor impact on BG levels,providing a potentially effective treatment for patients with diabetes[12].However,there is limited clinical evidence to compare the efficacy and glycaemic effects of retroauricular subperiosteal injection vs systemic intravenous GC administration in patients with SD and diabetes.This study aimed to explore the efficacy of retroauricular subperiosteal injection and systemic intravenous GC administration for treating patients with SD and DM and their effects on BG,providing a safer and more effective clinical treatment approach.展开更多
Study objective： to verify if the PAA （angle of the pubic arch） is a predictive data for the mode of delivery, the duration of labour and the expulsion period. Methods： we chose to measure the PAA in 100 women rec...Study objective： to verify if the PAA （angle of the pubic arch） is a predictive data for the mode of delivery, the duration of labour and the expulsion period. Methods： we chose to measure the PAA in 100 women recruited, chosen from the 38th to the 42th week of gestation. The method involves the use of two-dimensional transperineal ultrasound. Data collection took place over a period of time from May to September 2015. The ante-partum data taken into consideration were the patient’s name and surname, PAA, parity, age, height; those acquired in the post-partum provide for the calculation in minutes of the active phase of labor, the duration of the expulsion period, the execution of the amniorrhexis and the use of the synthetic oxytocin, the fetal cranial circumference, the fetal weight and the position of the presented part. Results： results discovered ultrasoundly 100 PAA, with an average value of 115.5° and we proceeded by evaluating 90 vaginal births. Infants at birth had a CC with an average value of 34.5 mm. By relating the two variables PAA and CC for the dependent variable “period expulsion in minutes”, confirming that the expulsive period depends on the relationship between PAA and CC. The average of the minutes of the expulsion period is 50＇ in women with a PAA 〉 111.5° and a CC 〉 34.5 mm while in women with an PAA 〉 111.5° the media time is 30＇ regardless of the CC. It has been shown that oxytocin and amniorrhexis reduce the minutes of the expulsion period only in a case of PAA 〉 111.5° and a CC 〉 34.5 mm bringing them from 60＇ to 23＇. Conclusion： the duration of the active phase of labor is not influenced by the PAA but it depends on external factors. In the case of the expulsion period, a predictability of the PAA on its duration is shown if the CC is taken into consideration; as regards the influence of the PAA on the method of delivery, it was not possible to carry out any analysis since all the useful cases had vaginal delivery.展开更多
Objective: to explore the influence of unprotected perineal delivery in delivery room nursing. Methods: the selection time was from January 2020 to January 2021, and the subjects were parturients who delivered natural...Objective: to explore the influence of unprotected perineal delivery in delivery room nursing. Methods: the selection time was from January 2020 to January 2021, and the subjects were parturients who delivered naturally through vagina. The above 100 parturients were divided into control group and experimental group by drawing lots, with 50 cases in each group. The control group was given the traditional delivery method, and the experimental group was given the unprotected perineal delivery method. The degree of perineal laceration, the time of the second stage of labor, the total amount of bleeding and the quality of life three months after delivery were compared between the two groups. Results: the degree of perineal laceration in the experimental group was lower than that in the control group (P < 0.05), and the data comparison was statistically significant. The duration of the second stage of labor and the amount of blood loss in the experimental group were less than those in the control group (P < 0.05), and the difference was statistically significant. 3months after delivery, two kinds of postpartum quality of life were investigated. The score of the experimental group was higher than that of the control group (P < 0.05), and the data difference is of comparative significance. Conclusion: unprotected perineal delivery method has less perineal damage to pregnant women, and is helpful to improve the quality of life of pregnant women after delivery. It is worthy of popularization and application.展开更多
This research was aim to develop novel cyclodextrin/chitosan （CD/CS） nanocarriers for insoluble drug delivery through the mild ionic gelation method previously developed by our lab. A series of different β- cyclode...This research was aim to develop novel cyclodextrin/chitosan （CD/CS） nanocarriers for insoluble drug delivery through the mild ionic gelation method previously developed by our lab. A series of different β- cyclodextrin （β-CD） derivatives were incorporated into CS nanoparticles including hydroxypropyl-β- cyclodextrin （HP-β-CD）, sulphobutylether-β-cyclodextrin （SB-β-CD）, and 2,6-di-O-methy-βcyclodex- trin （DM-β-CD）. Various process parameters for nanoparticle preparation and their effects on physicochemical properties of CD/CS nanoparticles were investigated, such as the type of CD derivatives, CD and CS concentrations, the mass ratio of CS to TPP （CS/TRP）, and pH values. In the optimal condition, CDICS nanoparticles were obtained in the size range of 215-276 nm and with the zeta potential from 30.22 mV to 35.79 mY. Moreover, the stability study showed that the incorporation of CD rendered the CD/CS nanocarriers more stable than CS nanoparticles in PBS buffer at pH 6.8. For their easy preparation and adjustable parameters in nanoparticle formation as well as the diversified hydrophobic core of CD derivatives, the novel CD/CS nanoparticles developed herein might represent an interesting and versatile drug delivery platform for a variety of poorly water-soluble drugs with different physicochemical properties.展开更多
Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,convention...Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,conventional transgene-mediated CRISPR/Cas delivery methods require a lengthy outcrossing process to eliminate CRISPR/Cas-associated sequences and produce transgene-free lines,circumventing regulatory restrictions related to transgenesis.RNA viral vectors offer a promising alternative for delivering genome engineering reagents,bypassing the need for tissue culture and avoiding transgenesis.Here,we discuss the bottlenecks in virus-mediated gene editing in plants,including virus exclusion from meristems,the requirement for a Cas-expressing plant,and concerns about virus host range and seed transmission.展开更多
Sustainability principles have become an integral part of the design and construc-tion process for many new construction projects. The selection of the project delivery method (PDM) is extremely important in the effec...Sustainability principles have become an integral part of the design and construc-tion process for many new construction projects. The selection of the project delivery method (PDM) is extremely important in the effective execution of the project, and plays a critical role in establishing communication and coordination between the key entities: owner, architect, and contractor. The goal of this paper is to hopefully serve as a starting point for further discussion to improve on the AEC industry’s current integration of sustainability principles in PDMs. The first step consists of an assessment of current project delivery systems from a sustainable design perspective. This is followed by a determination of the current limitations, and examination of the various disrup-tions in the industry. Various literature sources are analyzed to form a framework to discuss improvements and optimization strategies beyond the current system. Thereafter, proposed solutions are introduced at both stakeholders, as well as PDM scales. In this paper, the focus for the conducted analysis and proposed method-ologies is predicated on new construction projects instead of retrofits due to the resources available. However, the principles can similarly be applied to retro-fit scenarios as well, depending on the specific requirements of the individual project at play.展开更多
文摘Agrobacterium-mediated gene transformation in Brassica crops is a time-con-suming and labor-cost method （tissue culture and plant regeneration） in a genotype-depen-dent way. Here we described a new method 7n situ pistil delivery （ISPDy method with no tissue culture and adaptive to three Brassica species. Briefly this method can be stated as placement of a liquid drop containing binary vector DNA onto the cut surface of emasculated and artificial pollinated Brassica stigma. Transformation rate （Basta-resistant seedlings per 100 pistils） of different species ranged from 0.4% to 16.3%. Factors affecting transforma-tion success included tinning and buffer type. PCR and Southern blot confirmed transgenic events. Reciprocal cross showed reporter gene steadily inherited in nuclear transformed pattern. GUS histochemical assay of transgenic immature seeds showed stained embroys and endosperm in the same seed, indicating DNA delivered through style and integrated into Brassica seed/genome after double fertilization.
文摘Objective: This study focuses on the clinical observation of the impact of different treatment methods for gestational heart failure on delivery outcomes. Method: A total of 160 pregnant women with heart failure admitted to our hospital between October 2020 and October 2021 were selected as the study subjects. They were categorized based on delivery mode, delivery timing, heart failure control time, and cardiac function status. The delivery outcomes of the different groups were then compared. Result: In terms of delivery methods, the rate of neonatal asphyxia was higher following vaginal delivery than cesarean section. Regarding delivery timing, the neonatal mortality rate was lower for cesarean sections performed at 32-36 + 6 weeks compared to those conducted at 37-39 + 6 weeks. With respect to heart failure control time, the rates of neonatal asphyxia and pulmonary hyaline membrane disease were lower in the ≤ 48- hour group than in the > 48-hour group. From the perspective of cardiac function status, patients with cardiac function I- II exhibited relatively lower rates of neonatal asphyxia and perinatal mortality compared to those with cardiac function III-IV. The observed differences were statistically significant (P < 0.05). Conclusion: For patients with gestational heart failure, cesarean section is the recommended mode of delivery, with the optimal timing being between 32 and 36+6 weeks of pregnancy. During cesarean section, the timing of delivery should be carefully selected based on the mother’s cardiac function status.
基金supported by the National Natural Science Foundation of China(30771428)
文摘RNAi trigged by dsRNA not only facilitates the development of molecular biology, but also initiates a new way for pest control by silence of fatal genes. However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one- time injection of larvae with dsRNA could reduce the pupation rate by 43.0% and enhance larva mortality by 11.7%. One- time ingestion of dsRNA did not result in any significant effect on phenotype. Continuous ingestion of in vitro synthesized dsRNA by refreshing the bait diet every day caused 40.4% decrease in successful pupation and 10.0% increase in larval mortality, which was similar as one-time injection. The most efficient method was found to be the continuous ingestion of the bacteria containing dsRNA expressed, which reduced the rate of pupation by 68.7% and enhanced the larval mortality by 34.1%. Further analysis found that dsRNA was degraded faster in midgut juice than in hemolymph. However, the cell of bacteria could protect dsRNA and delay the degradation in the midgut juice of H. armigera. These results throw light on the application of dsRNA in pest management with proper ways.
文摘The selection of the project delivery method(PDM)for any project is critical—it establishes the communication,coordination,and contractual interfaces between the owner,contractor,and designer.With an increase in the number of green design projects,understanding the relationship between the PDM and green design is paramount to project and contract management.While it is reasonable to assume that a relationship between green design and design-build(DB)exists since both theoretically are intended to foster an integrated,holistic,team-based collaborative project;it is also appropriate to understand the relationship between green design and other PDMs.This preliminary research examined possible relationships between design-bid-build(DBB),construction management(CM),and DB PDMs and green design with the goal of beginning to identify potential positive correspondence between them.To develop an initial understanding,two main tasks were completed.First,existing published research was evaluated to reveal aspects of projects and PDMs independent of green design.Second,the research collected primarily qualitative information by conducting structured interviews with approximately twenty-five individuals,including owners,contractors,and designers involved in completed green design projects,mainly in the public sector.Upon completion of the structured interviews,responses from the interviews were reviewed for green design project characteristics.Additionally,the interviews provided insight on the initial understanding of the current state of knowledge and experience in green design and PDMs.After the two main tasks were completed,both were evaluated for commonalities.Preliminary results found that seven green design project characteristics emerged that in some cases related to a specific PDM,but in other cases were independent of a PDM.Future research will quantitatively investigate the relationship between green design projects,PDMs,and success factors.
文摘Diabetic patients often suffer higher orthopedic implant failure rates due to high blood sugar causing inflammation and oxidative stress.While molecular hydrogen(H_(2))shows anti-inflammatory and antioxidant promise,current delivery methods lack precision for sustained implantsite treatment.A collaborative team led by Prof.
基金supported by the National Natural Science Foundation of China,No.82002645China Postdoctoral Science Foundation,No.2022M722321Jiangsu Funding Program for Excellent Postdoctoral Talent,No.2022ZB552(all to YH)。
文摘Spinal cord injuries have overwhelming physical and occupational implications for patients.Moreover,the extensive and long-term medical care required for spinal cord injury significantly increases healthcare costs and resources,adding a substantial burden to the healthcare system and patients'families.In this context,chondroitinase ABC,a bacterial enzyme isolated from Proteus vulgaris that is modified to facilitate expression and secretion in mammals,has emerged as a promising therapeutic agent.It works by degrading chondroitin sulfate proteoglycans,cleaving the glycosaminoglycanchains of chondroitin sulfate proteoglycans into soluble disaccharides or tetrasaccharides.Chondroitin sulfate proteoglycans are potent axon growth inhibitors and principal constituents of the extracellular matrix surrounding glial and neuronal cells attached to glycosaminoglycan chains.Chondroitinase ABC has been shown to play an effective role in promoting recovery from acute and chronic spinal cord injury by improving axonal regeneration and sprouting,enhancing the plasticity of perineuronal nets,inhibiting neuronal apoptosis,and modulating immune responses in various animal models.In this review,we introduce the classification and pathological mechanisms of spinal cord injury and discuss the pathophysiological role of chondroitin sulfate proteoglycans in spinal cord injury.We also highlight research advancements in spinal cord injury treatment strategies,with a focus on chondroitinase ABC,and illustrate how improvements in chondroitinase ABC stability,enzymatic activity,and delivery methods have enhanced injured spinal cord repair.Furthermore,we emphasize that combination treatment with chondroitinase ABC further enhances therapeutic efficacy.This review aimed to provide a comprehensive understanding of the current trends and future directions of chondroitinase ABC-based spinal cord injury therapies,with an emphasis on how modern technologies are accelerating the optimization of chondroitinase ABC development.
文摘BACKGROUND Managing sudden deafness(SD)in patients with diabetes mellitus(DM)is partic-ularly challenging due to the heightened risk of adverse effects associated with systemic drug administration.This study explores the potential of retroauricular subperiosteal injection as a localized drug delivery method for a more effective and safe treatment.AIM To compare the efficacy of retroauricular subperiosteal injection vs systemic intravenous glucocorticoid(GC)administration for SD in patients with DM and assess the effects on blood glucose levels.METHODS A total of 128 cases of type 2 DM(T2DM)with SD diagnosed and treated in Zibo Central Hospital from February 2021 to July 2023 were divided into two groups:An observation group(66 cases receiving retroauricular subperiosteal injection of methylprednisolone)and a control group(62 cases receiving systemic intravenous administration of methylprednisolone).The two groups were compared in terms of therapeutic efficacy,hearing recovery,blood glucose level changes,and incidence of adverse reactions.Binary logistic regression was used to analyze the factors affecting therapeutic efficacy.RESULTS The observation group showed a significantly higher total effective rate(90.91%)compared with the control group(75.81%,P<0.05).Additionally,pure-tone hearing threshold,fasting plasma glucose,and 2-hour postprandial blood glucose were significantly lower in the observation group compared with the control group(P<0.05).The incidence of adverse reactions was also lower in the observation group than in the control group(7.58%vs 22.58%,P<0.05).A T2DM course longer than 5 years and systemic intravenous GC administration were identified as independent risk factors for treatment inefficacy(P<0.05).INTRODUCTION Sudden deafness(SD)is a clinical emergency characterized by rapid-onset hearing loss that is often accompanied by clinical symptoms such as tinnitus and vertigo[1].Although its pathogenesis remains unclear,it is supposedly associated with factors,including inner ear microcirculation disorders,autoimmune diseases,and viral infections[2,3].Patients with diabetes are particularly susceptible to microvascular complications due to poor long-term glycemic control,affecting the ear’s microcirculation,subsequently increasing the risk of SD[4].Type 2 diabetes mellitus(T2DM),a chronic metabolic disease,causes multiple microvascular damages throughout the body,complicating SD treatment in patients with diabetes[5,6].Inner ear microcirculation disturbances in patients with diabetes may exacerbate the risk of SD,and its pathological process may be related to vascular endothelial dysfunction,inflammatory reactions,and hemorrhological changes caused by diabetes mellitus(DM)[7].Current SD treatments include glucocorticoids(GCs),vasodilators,and hyperbaric oxygen therapy[8].GCs are widely used due to their anti-inflammatory and immunosuppressive effects[9].However,systemic GCs may cause blood glucose(BG)fluctuations and even increase the risk of complications in patients with DM,limiting their clinical use in this population[10].Therefore,there is a compelling and immediate need for a local alternative solution that minimally affects the metabolic mechanisms.In recent years,retroauricular subperiosteal injection has emerged as a localized administration modality for treating SD[11].This method allows drugs to directly act on the inner ear,avoiding the side effects of systemic administration and having a minor impact on BG levels,providing a potentially effective treatment for patients with diabetes[12].However,there is limited clinical evidence to compare the efficacy and glycaemic effects of retroauricular subperiosteal injection vs systemic intravenous GC administration in patients with SD and diabetes.This study aimed to explore the efficacy of retroauricular subperiosteal injection and systemic intravenous GC administration for treating patients with SD and DM and their effects on BG,providing a safer and more effective clinical treatment approach.
文摘Study objective： to verify if the PAA （angle of the pubic arch） is a predictive data for the mode of delivery, the duration of labour and the expulsion period. Methods： we chose to measure the PAA in 100 women recruited, chosen from the 38th to the 42th week of gestation. The method involves the use of two-dimensional transperineal ultrasound. Data collection took place over a period of time from May to September 2015. The ante-partum data taken into consideration were the patient’s name and surname, PAA, parity, age, height; those acquired in the post-partum provide for the calculation in minutes of the active phase of labor, the duration of the expulsion period, the execution of the amniorrhexis and the use of the synthetic oxytocin, the fetal cranial circumference, the fetal weight and the position of the presented part. Results： results discovered ultrasoundly 100 PAA, with an average value of 115.5° and we proceeded by evaluating 90 vaginal births. Infants at birth had a CC with an average value of 34.5 mm. By relating the two variables PAA and CC for the dependent variable “period expulsion in minutes”, confirming that the expulsive period depends on the relationship between PAA and CC. The average of the minutes of the expulsion period is 50＇ in women with a PAA 〉 111.5° and a CC 〉 34.5 mm while in women with an PAA 〉 111.5° the media time is 30＇ regardless of the CC. It has been shown that oxytocin and amniorrhexis reduce the minutes of the expulsion period only in a case of PAA 〉 111.5° and a CC 〉 34.5 mm bringing them from 60＇ to 23＇. Conclusion： the duration of the active phase of labor is not influenced by the PAA but it depends on external factors. In the case of the expulsion period, a predictability of the PAA on its duration is shown if the CC is taken into consideration; as regards the influence of the PAA on the method of delivery, it was not possible to carry out any analysis since all the useful cases had vaginal delivery.
文摘Objective: to explore the influence of unprotected perineal delivery in delivery room nursing. Methods: the selection time was from January 2020 to January 2021, and the subjects were parturients who delivered naturally through vagina. The above 100 parturients were divided into control group and experimental group by drawing lots, with 50 cases in each group. The control group was given the traditional delivery method, and the experimental group was given the unprotected perineal delivery method. The degree of perineal laceration, the time of the second stage of labor, the total amount of bleeding and the quality of life three months after delivery were compared between the two groups. Results: the degree of perineal laceration in the experimental group was lower than that in the control group (P < 0.05), and the data comparison was statistically significant. The duration of the second stage of labor and the amount of blood loss in the experimental group were less than those in the control group (P < 0.05), and the difference was statistically significant. 3months after delivery, two kinds of postpartum quality of life were investigated. The score of the experimental group was higher than that of the control group (P < 0.05), and the data difference is of comparative significance. Conclusion: unprotected perineal delivery method has less perineal damage to pregnant women, and is helpful to improve the quality of life of pregnant women after delivery. It is worthy of popularization and application.
基金financially supported by Postdoctoral Science Foundation of China (No. 2014M550222)Shanghai Postdoctoral Sustentation Fund (No. 14R21410500)+2 种基金the support from School of Pharmacy, Fudan University & the Open Project Program of Key Lab of Smart Drug Delivery (Fudan University), Ministry of Education (No. SDD2014-2)State Key Laboratory of Molecular Engineering of Polymers (Fudan University, No. K2015-15)the Fundamental Research Funds for the Central Universities (Nos. 22A201514055 and WY1213013 ECUST)
文摘This research was aim to develop novel cyclodextrin/chitosan （CD/CS） nanocarriers for insoluble drug delivery through the mild ionic gelation method previously developed by our lab. A series of different β- cyclodextrin （β-CD） derivatives were incorporated into CS nanoparticles including hydroxypropyl-β- cyclodextrin （HP-β-CD）, sulphobutylether-β-cyclodextrin （SB-β-CD）, and 2,6-di-O-methy-βcyclodex- trin （DM-β-CD）. Various process parameters for nanoparticle preparation and their effects on physicochemical properties of CD/CS nanoparticles were investigated, such as the type of CD derivatives, CD and CS concentrations, the mass ratio of CS to TPP （CS/TRP）, and pH values. In the optimal condition, CDICS nanoparticles were obtained in the size range of 215-276 nm and with the zeta potential from 30.22 mV to 35.79 mY. Moreover, the stability study showed that the incorporation of CD rendered the CD/CS nanocarriers more stable than CS nanoparticles in PBS buffer at pH 6.8. For their easy preparation and adjustable parameters in nanoparticle formation as well as the diversified hydrophobic core of CD derivatives, the novel CD/CS nanoparticles developed herein might represent an interesting and versatile drug delivery platform for a variety of poorly water-soluble drugs with different physicochemical properties.
基金supported by the Governor's University Research Initiative programfrom the state of Texas.
文摘Unlocking the full potential of genome editing for crop improvement has been hindered by inefficient reagent delivery methods and the reliance on tissue culture for generating gene-edited plants.In addition,conventional transgene-mediated CRISPR/Cas delivery methods require a lengthy outcrossing process to eliminate CRISPR/Cas-associated sequences and produce transgene-free lines,circumventing regulatory restrictions related to transgenesis.RNA viral vectors offer a promising alternative for delivering genome engineering reagents,bypassing the need for tissue culture and avoiding transgenesis.Here,we discuss the bottlenecks in virus-mediated gene editing in plants,including virus exclusion from meristems,the requirement for a Cas-expressing plant,and concerns about virus host range and seed transmission.
文摘Sustainability principles have become an integral part of the design and construc-tion process for many new construction projects. The selection of the project delivery method (PDM) is extremely important in the effective execution of the project, and plays a critical role in establishing communication and coordination between the key entities: owner, architect, and contractor. The goal of this paper is to hopefully serve as a starting point for further discussion to improve on the AEC industry’s current integration of sustainability principles in PDMs. The first step consists of an assessment of current project delivery systems from a sustainable design perspective. This is followed by a determination of the current limitations, and examination of the various disrup-tions in the industry. Various literature sources are analyzed to form a framework to discuss improvements and optimization strategies beyond the current system. Thereafter, proposed solutions are introduced at both stakeholders, as well as PDM scales. In this paper, the focus for the conducted analysis and proposed method-ologies is predicated on new construction projects instead of retrofits due to the resources available. However, the principles can similarly be applied to retro-fit scenarios as well, depending on the specific requirements of the individual project at play.
