At ITU Telecom Africa 2004,Telecom Egyptofficially announced that it had selected ZTECorporation,the leading telecom solutionsprovider in the world,to be its global strategicpartner,and that ZTE would undertake thecon...At ITU Telecom Africa 2004,Telecom Egyptofficially announced that it had selected ZTECorporation,the leading telecom solutionsprovider in the world,to be its global strategicpartner,and that ZTE would undertake theconstruction of its CDMA WLL network anddeliver full services to Telecom Egypt.Akil Beshir,Chairman of Telecom展开更多
China’s next-generation geostationary meteorological satellite FY-4 obtained the first images and data on February 9 and20 after two-months of in-orbit commissioning.The first FY-4satellite was developed by SAST and ...China’s next-generation geostationary meteorological satellite FY-4 obtained the first images and data on February 9 and20 after two-months of in-orbit commissioning.The first FY-4satellite was developed by SAST and was launched into space atop a LM-3B launch vehicle on December 11,2016 and positioned at 99.5°E over the equator on December 17.The satellite,designated as FY-4A officially,carries payloads展开更多
Dr. Craig Mello, the Nobel Prize Winner of 2006 in Physiology or Medicine, delivered a speech entitled Return to The RNA World： Rethinking Gene Expression, Evolution, and Medicine at Tsinghua on March 26, 2007.
In May 2017,China hosted the First Belt and Road Forum for International Cooperation(BRF).The Second BRF,to be held in Beijing in late April under the theme Belt and Road Cooperation:Ihaping a Brighter Shared Future,i...In May 2017,China hosted the First Belt and Road Forum for International Cooperation(BRF).The Second BRF,to be held in Beijing in late April under the theme Belt and Road Cooperation:Ihaping a Brighter Shared Future,is expected to send a message of further strengthening international economic cooperation,promoting global growth and building an open global economy.Ahead of the eve nt,Beijing Review prese nts a cover story package,including experts'observations as well as reports from companies on the ground,to demonstrate how this initiative has gained traction over the years.展开更多
University of Southern California （USC） Professor Kai Hwang delivered the Tsinghua Global Vision Lecture “Global Advances in Computer and Information Technology： Can China Take the Lead？” in the FIT Lecture Hall...University of Southern California （USC） Professor Kai Hwang delivered the Tsinghua Global Vision Lecture “Global Advances in Computer and Information Technology： Can China Take the Lead？” in the FIT Lecture Hall on January 6, 2009. During his lecture, Professor Hwang shared his ideas about several global advances in computer, networking, and information technology and discussed the great challenges yet to be confronted in information and technology in academia and in industry and the building of world-class universities. In his address,展开更多
On New Year’s Eve,Chinese President Xi Jinping delivered his 2025 New Year message through China Media Group and the Internet.Edited excerpts of the message follow:Nurtured by our 5,000-plus years of continuous civil...On New Year’s Eve,Chinese President Xi Jinping delivered his 2025 New Year message through China Media Group and the Internet.Edited excerpts of the message follow:Nurtured by our 5,000-plus years of continuous civilisation,our country,China,is engraved not only on the bottom of the ancient bronze ritual wine vessel of He Zun,but also in the heart of every Chinese.展开更多
Autophagy is well-known for delivering cargo materials to lysosomes for proteolytic digestion.Recently,autophagy has emerged as a key mechanism in unconventional protein secretion(UPS).This perspective introduces unco...Autophagy is well-known for delivering cargo materials to lysosomes for proteolytic digestion.Recently,autophagy has emerged as a key mechanism in unconventional protein secretion(UPS).This perspective introduces unconventional secretion pathways,focusing on secretory autophagy and its role in secreting protein aggregates associated with neurodegenerative disorders.We also explore additional neuronal functions of secretory autophagy beyond the release of protein aggregates.We propose autophagosomes as transport organelles that deliver cargo material directly from the endoplasmatic reticulum(ER)to the plasma membrane rather than solely to lysosomes.展开更多
This study presents a comparative analysis of optimisation strategies for designing hull shapes of Autonomous Underwater Vehicles(AUVs),paying special attention to drag,lift-to-drag ratio,and delivered power.A fully i...This study presents a comparative analysis of optimisation strategies for designing hull shapes of Autonomous Underwater Vehicles(AUVs),paying special attention to drag,lift-to-drag ratio,and delivered power.A fully integrated optimisation framework is developed accordingly,combining a single-objective Genetic Algorithm(GA)for design parameter generation,Computer-Aided Geometric Design(CAGD)for the creation of hull geometries and associated fluid domains,and a Reynolds-Averaged Navier-Stokes(RANS)solver for evaluating hydrodynamic performance metrics.This unified approach eliminates manual intervention,enabling automated determination of optimal hull configurations.Three distinct optimisation problems are addressed using the proposed methodology.First,the drag minimisation of a reference afterbody geometry(A1)at zero angle of attack is performed under constraints of fixed length and internal volume for various flow velocities spanning the range from 0.5 to 15 m/s.Second,the lift-to-drag ratio of A1 is maximised at a 6°angle of attack,maintaining constant total length and internal volume.Third,delivered power is minimised for A1 at a 0°angle of attack.The comparative analysis of results from all three optimisation cases reveals hull shapes with practical design significance.Notably,the shape optimised for minimum delivered power outperforms the other two across a range of velocities.Specifically,it achieves reductions in required power by 7.6%,7.8%,10.2%,and 13.04%at velocities of 0.5,1.0,1.5,and 2.152 m/s,respectively.展开更多
Companies can add Gen2 support on existing Zebra RFID printer/encoders while continuing to use Gen 1 and other protocols: no new hardware required. Vernon Hills,Ⅲ., December 14,2005-Zebra Technologies (Nasdaq:ZBRA),a...Companies can add Gen2 support on existing Zebra RFID printer/encoders while continuing to use Gen 1 and other protocols: no new hardware required. Vernon Hills,Ⅲ., December 14,2005-Zebra Technologies (Nasdaq:ZBRA),a leading manufacturer of radio frequency identification (RFID) printer/encoders worldwide,展开更多
On Sept 20,the 2022 World Manufacturing Convention successfully opened in Hefei,Anhui province.Zheng Shanjie,secretary of the Communist Party of China’s Anhui provincial committee;Wang Jiangping,a member of the Party...On Sept 20,the 2022 World Manufacturing Convention successfully opened in Hefei,Anhui province.Zheng Shanjie,secretary of the Communist Party of China’s Anhui provincial committee;Wang Jiangping,a member of the Party group and vice-minister of the Ministry of Industry and Information Technology;and Zhang Wei,a member of the CPC Shanghai Municipal Committee’s Standing Committee and vice-mayor of Shanghai,delivered speeches at the opening ceremony.展开更多
Ticona Engineering Polymers will show-case its local manufacturing capability,de-sign and application development support,and comprehensive technical and processing knowledge at Chinaplas 2008 in the Shanghai New Inte...Ticona Engineering Polymers will show-case its local manufacturing capability,de-sign and application development support,and comprehensive technical and processing knowledge at Chinaplas 2008 in the Shanghai New International Expo Centre here April 17 to 20."In China and the rest of Asia,Ticona is strategically positioning itself to deliver theright material to meet our customer appli-cation requirements and the right people to deliver the best solutions fast,"said Sandra Beach Lin,president of Ticona."The key is our people,who not only are the best technical minds,but also the lead-展开更多
On July 4,2024,Chinese President Xi Jinping attends the“Shanghai Cooperation Organization Plus”meeting in Astana,Kazakhstan,and delivers a speech titled“Joining Hands to Build a More Beautiful Home of the Shanghai ...On July 4,2024,Chinese President Xi Jinping attends the“Shanghai Cooperation Organization Plus”meeting in Astana,Kazakhstan,and delivers a speech titled“Joining Hands to Build a More Beautiful Home of the Shanghai Cooperation Organization”.展开更多
FOCAC sends the powerful message that China and Africa are pursuing joint development,modernisation and growth.The recently held 2024 Summit of the Forum on China-Africa Cooperation(FOCAC)in Beijing under the theme Jo...FOCAC sends the powerful message that China and Africa are pursuing joint development,modernisation and growth.The recently held 2024 Summit of the Forum on China-Africa Cooperation(FOCAC)in Beijing under the theme Joining Hands to Advance Modernisation and Build a High-Level China-Africa Community with a Shared Future unequivocally demonstrated that FOCAC remains the most important flagship collaborative platform between China and Africa and continues to deliver wide-ranging.展开更多
China’s achievements in high-quality development,including poverty alleviation,decarbonisation,clean energy,and digitalisation,together with progress in other areas,have significantly contributed to the global sustai...China’s achievements in high-quality development,including poverty alleviation,decarbonisation,clean energy,and digitalisation,together with progress in other areas,have significantly contributed to the global sustainable development agenda.The concept of“high-quality development”was first introduced in the report delivered by Chinese President Xi Jinping at the 19th National Congress of the Communist Party of China in 2017.He emphasised that“China’s economy has been transitioning from a phase of rapid growth to a stage of high-quality development.”展开更多
Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases...Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases.Impact Statement:This study develops a new technology that can accurately visualize the thrombus and deliver drugs with controllable properties to diagnose and treat thrombotic diseases.Introduction:Thrombotic diseases are a serious threat to human life and health.The diagnosis and treatment of thrombotic diseases have always been a challenge.In recent years,nanomedicine has brought new ideas and new methods for the theranostics of thrombotic diseases.However,there are also many problems need to be solved,such as biosafety and stability of nanocarriers,early diagnosis,and timely treatment of thrombotic diseases,difficulty in clinical translation.Methods:The S1P@CD-PLGA-rtPA nanobubbles(NBs)were prepared by integrating sulfur hexafluoride(SF6)-loaded poly(D,L-lactide-co-glycolide)(PLGA)NBs,cyclodextrin(CD),sphingosine-1-phosphate(S1P),and recombinant tissue plasminogen activator(rtPA).Results:S1P@CD-PLGA-rtPA NBs had rapid and excellent thrombosis targeting imaging performance based on the specific interaction of S1P-S1PR1(sphingosine-1-phosphate receptor 1).Furthermore,S1P@CD-PLGA-rtPA NBs that specifically targeting to the thrombosis regions could also respond to external ultrasound to achieve accurate and efficient delivery of rtPA to enhance the thrombolysis effectiveness and efficiency.Conclusion:This study proposes a new idea and strategy of targeting thrombus in rats via the specific interaction of S1P-S1PR1.On this basis,the acoustic response properties of bubble carriers could be fully utilized by combining thrombus-specific targeted imaging and ultrasound-mediated drug delivery for effective thrombolysis,which is expected to be applied in targeted diagnosis and treatment of thrombotic diseases in the future.展开更多
Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stituti...Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.展开更多
BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of ...BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector's function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system.展开更多
Mr.Chairman,Ladies and Gentlemen,It is a great honour and pleasure for me tohave the opportunity to deliver this lecture on“How Acupuncture-Moxibustion Should Go For-ward into the 21st Century”.In 1978,the Internati...Mr.Chairman,Ladies and Gentlemen,It is a great honour and pleasure for me tohave the opportunity to deliver this lecture on“How Acupuncture-Moxibustion Should Go For-ward into the 21st Century”.In 1978,the International Conference onPrimary Health Care held in Alma-Ata recog-nized the potential of traditional medicine in ex-tending primary health care to underserved pop-ulations.In many developing countries,in fact,people have always used both traditional展开更多
BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Pati...BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Patients with AKI requiring CRRT in an ICU were randomly divided into two groups for crossover trial,i.e.,groups A and B.Patients in the group A were firstly treated with continuous veno-venous hemofiltration(CVVH),followed by continuous veno-venous hemodiafiltration(CVVHDF).Patients in the group B were firstly treated with CVVHDF followed by CVVH.Delivered doses of solutes with different molecular weights at the indicated time points between groups were compared.A correlation analysis between the delivered dose and pre-filter pressure(P_(PRE))and transmembrane pressure(P_(TM))was performed.Receiver operating characteristic(ROC)curves were constructed to evaluate the accuracy of P_(TM) as an indicator for filter replacement.RESULTS:A total of 50 cases were analyzed,27 in the group A and 23 in the group B.Delivered doses of different molecular-weight solutes significantly decreased before changing the filter in both modalities,compared with those at the initiation of treatment(all P<0.05).In the late stage of CRRT,the possible rebound of serum medium-molecular-weight solute concentration was observed.P_(TM) was negatively correlated with the delivered dose of medium-molecular-weight solute in both modalities.The threshold for predicting the rebound of serum concentration of medium-molecularweight solute by P_(TM) was 146.5 mm Hg(1 mm Hg=0.133 k Pa).CONCLUSIONS:The filter can be used as long as possible within the manufacturer’s safe use time limits to remove small-molecular-weight solutes.P_(TM) of 146.5 mm Hg may be an optimal indicator for changing the filter in CRRT therapies to remove medium-molecular-weight solutes.展开更多
文摘At ITU Telecom Africa 2004,Telecom Egyptofficially announced that it had selected ZTECorporation,the leading telecom solutionsprovider in the world,to be its global strategicpartner,and that ZTE would undertake theconstruction of its CDMA WLL network anddeliver full services to Telecom Egypt.Akil Beshir,Chairman of Telecom
文摘China’s next-generation geostationary meteorological satellite FY-4 obtained the first images and data on February 9 and20 after two-months of in-orbit commissioning.The first FY-4satellite was developed by SAST and was launched into space atop a LM-3B launch vehicle on December 11,2016 and positioned at 99.5°E over the equator on December 17.The satellite,designated as FY-4A officially,carries payloads
文摘Dr. Craig Mello, the Nobel Prize Winner of 2006 in Physiology or Medicine, delivered a speech entitled Return to The RNA World： Rethinking Gene Expression, Evolution, and Medicine at Tsinghua on March 26, 2007.
文摘In May 2017,China hosted the First Belt and Road Forum for International Cooperation(BRF).The Second BRF,to be held in Beijing in late April under the theme Belt and Road Cooperation:Ihaping a Brighter Shared Future,is expected to send a message of further strengthening international economic cooperation,promoting global growth and building an open global economy.Ahead of the eve nt,Beijing Review prese nts a cover story package,including experts'observations as well as reports from companies on the ground,to demonstrate how this initiative has gained traction over the years.
文摘University of Southern California （USC） Professor Kai Hwang delivered the Tsinghua Global Vision Lecture “Global Advances in Computer and Information Technology： Can China Take the Lead？” in the FIT Lecture Hall on January 6, 2009. During his lecture, Professor Hwang shared his ideas about several global advances in computer, networking, and information technology and discussed the great challenges yet to be confronted in information and technology in academia and in industry and the building of world-class universities. In his address,
文摘On New Year’s Eve,Chinese President Xi Jinping delivered his 2025 New Year message through China Media Group and the Internet.Edited excerpts of the message follow:Nurtured by our 5,000-plus years of continuous civilisation,our country,China,is engraved not only on the bottom of the ancient bronze ritual wine vessel of He Zun,but also in the heart of every Chinese.
基金supported by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)grant LU 2347/3-1(to PL).
文摘Autophagy is well-known for delivering cargo materials to lysosomes for proteolytic digestion.Recently,autophagy has emerged as a key mechanism in unconventional protein secretion(UPS).This perspective introduces unconventional secretion pathways,focusing on secretory autophagy and its role in secreting protein aggregates associated with neurodegenerative disorders.We also explore additional neuronal functions of secretory autophagy beyond the release of protein aggregates.We propose autophagosomes as transport organelles that deliver cargo material directly from the endoplasmatic reticulum(ER)to the plasma membrane rather than solely to lysosomes.
文摘This study presents a comparative analysis of optimisation strategies for designing hull shapes of Autonomous Underwater Vehicles(AUVs),paying special attention to drag,lift-to-drag ratio,and delivered power.A fully integrated optimisation framework is developed accordingly,combining a single-objective Genetic Algorithm(GA)for design parameter generation,Computer-Aided Geometric Design(CAGD)for the creation of hull geometries and associated fluid domains,and a Reynolds-Averaged Navier-Stokes(RANS)solver for evaluating hydrodynamic performance metrics.This unified approach eliminates manual intervention,enabling automated determination of optimal hull configurations.Three distinct optimisation problems are addressed using the proposed methodology.First,the drag minimisation of a reference afterbody geometry(A1)at zero angle of attack is performed under constraints of fixed length and internal volume for various flow velocities spanning the range from 0.5 to 15 m/s.Second,the lift-to-drag ratio of A1 is maximised at a 6°angle of attack,maintaining constant total length and internal volume.Third,delivered power is minimised for A1 at a 0°angle of attack.The comparative analysis of results from all three optimisation cases reveals hull shapes with practical design significance.Notably,the shape optimised for minimum delivered power outperforms the other two across a range of velocities.Specifically,it achieves reductions in required power by 7.6%,7.8%,10.2%,and 13.04%at velocities of 0.5,1.0,1.5,and 2.152 m/s,respectively.
文摘Companies can add Gen2 support on existing Zebra RFID printer/encoders while continuing to use Gen 1 and other protocols: no new hardware required. Vernon Hills,Ⅲ., December 14,2005-Zebra Technologies (Nasdaq:ZBRA),a leading manufacturer of radio frequency identification (RFID) printer/encoders worldwide,
文摘On Sept 20,the 2022 World Manufacturing Convention successfully opened in Hefei,Anhui province.Zheng Shanjie,secretary of the Communist Party of China’s Anhui provincial committee;Wang Jiangping,a member of the Party group and vice-minister of the Ministry of Industry and Information Technology;and Zhang Wei,a member of the CPC Shanghai Municipal Committee’s Standing Committee and vice-mayor of Shanghai,delivered speeches at the opening ceremony.
文摘Ticona Engineering Polymers will show-case its local manufacturing capability,de-sign and application development support,and comprehensive technical and processing knowledge at Chinaplas 2008 in the Shanghai New International Expo Centre here April 17 to 20."In China and the rest of Asia,Ticona is strategically positioning itself to deliver theright material to meet our customer appli-cation requirements and the right people to deliver the best solutions fast,"said Sandra Beach Lin,president of Ticona."The key is our people,who not only are the best technical minds,but also the lead-
文摘On July 4,2024,Chinese President Xi Jinping attends the“Shanghai Cooperation Organization Plus”meeting in Astana,Kazakhstan,and delivers a speech titled“Joining Hands to Build a More Beautiful Home of the Shanghai Cooperation Organization”.
文摘FOCAC sends the powerful message that China and Africa are pursuing joint development,modernisation and growth.The recently held 2024 Summit of the Forum on China-Africa Cooperation(FOCAC)in Beijing under the theme Joining Hands to Advance Modernisation and Build a High-Level China-Africa Community with a Shared Future unequivocally demonstrated that FOCAC remains the most important flagship collaborative platform between China and Africa and continues to deliver wide-ranging.
文摘China’s achievements in high-quality development,including poverty alleviation,decarbonisation,clean energy,and digitalisation,together with progress in other areas,have significantly contributed to the global sustainable development agenda.The concept of“high-quality development”was first introduced in the report delivered by Chinese President Xi Jinping at the 19th National Congress of the Communist Party of China in 2017.He emphasised that“China’s economy has been transitioning from a phase of rapid growth to a stage of high-quality development.”
基金supported by the project of National Natural Science Foundation of China(81971750)Natural Science Foundation of Jiangsu Province(Grant No.BK20222002)+1 种基金National Key Research and Development Program of China(2018YFA0704103)Project 333 of Jiangsu Province.
文摘Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases.Impact Statement:This study develops a new technology that can accurately visualize the thrombus and deliver drugs with controllable properties to diagnose and treat thrombotic diseases.Introduction:Thrombotic diseases are a serious threat to human life and health.The diagnosis and treatment of thrombotic diseases have always been a challenge.In recent years,nanomedicine has brought new ideas and new methods for the theranostics of thrombotic diseases.However,there are also many problems need to be solved,such as biosafety and stability of nanocarriers,early diagnosis,and timely treatment of thrombotic diseases,difficulty in clinical translation.Methods:The S1P@CD-PLGA-rtPA nanobubbles(NBs)were prepared by integrating sulfur hexafluoride(SF6)-loaded poly(D,L-lactide-co-glycolide)(PLGA)NBs,cyclodextrin(CD),sphingosine-1-phosphate(S1P),and recombinant tissue plasminogen activator(rtPA).Results:S1P@CD-PLGA-rtPA NBs had rapid and excellent thrombosis targeting imaging performance based on the specific interaction of S1P-S1PR1(sphingosine-1-phosphate receptor 1).Furthermore,S1P@CD-PLGA-rtPA NBs that specifically targeting to the thrombosis regions could also respond to external ultrasound to achieve accurate and efficient delivery of rtPA to enhance the thrombolysis effectiveness and efficiency.Conclusion:This study proposes a new idea and strategy of targeting thrombus in rats via the specific interaction of S1P-S1PR1.On this basis,the acoustic response properties of bubble carriers could be fully utilized by combining thrombus-specific targeted imaging and ultrasound-mediated drug delivery for effective thrombolysis,which is expected to be applied in targeted diagnosis and treatment of thrombotic diseases in the future.
基金The National Basic Research Program of China (973 Program) under contract No. 2012CB114403General Program of National Natural Science Foundation of China to Dr. Fuhua Li under contract No. 31072203+1 种基金National Hightech Research and Development Program under contract Nos 2012AA10A404 and 2012AA092205China Agriculture Research System under contract No. CARS-47
文摘Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
基金This study was supported by grants from the National Natural Science Foun-dation of China( No:30472251 )and the Shanxi Youth Science Fund ( No.020011028).
文摘BACKGROUND: The development of a harmless and effi- cient nonviral gene delivery system that can facilitate the penetration of nucleic acids through the plasma membrane is a key to successful gene therapy. The aim of this study was to test a nonviral gene transferring vector's function of delivering DNA into liver cells to provide an important clue for gene transfer in liver gene therapy. METHODS: The complex of DNA and DNA delivering protein was injected into mice through their tail veins. Then the mice were killed and their liver tissue was sec- tioned. The gene transferring results were detected using a confocal laser scanning microscope. RESULTS: Fluorescence analysis indicated that both DNA- membrane penetrating peptide (MPP) complex and DNA- hepatocyte specific receptor binding domain ( HSRBD) - MPP complex could go into liver cells. The fluorescence value of liver cells in the DNA-HSRBD-MPP group was higher than that in the DNA-MPP group. CONCLUSIONS; MPP can successfully deliver DNA and protein into cells, and MPP with a HSRBD can specifically deliver DNA into liver cells. These have laid a foundation for further study on the nonviral liver cell gene delivering system.
文摘Mr.Chairman,Ladies and Gentlemen,It is a great honour and pleasure for me tohave the opportunity to deliver this lecture on“How Acupuncture-Moxibustion Should Go For-ward into the 21st Century”.In 1978,the International Conference onPrimary Health Care held in Alma-Ata recog-nized the potential of traditional medicine in ex-tending primary health care to underserved pop-ulations.In many developing countries,in fact,people have always used both traditional
基金supported by Kunshan Science and Technology Special Fund(Social Development Category,KS18040)。
文摘BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Patients with AKI requiring CRRT in an ICU were randomly divided into two groups for crossover trial,i.e.,groups A and B.Patients in the group A were firstly treated with continuous veno-venous hemofiltration(CVVH),followed by continuous veno-venous hemodiafiltration(CVVHDF).Patients in the group B were firstly treated with CVVHDF followed by CVVH.Delivered doses of solutes with different molecular weights at the indicated time points between groups were compared.A correlation analysis between the delivered dose and pre-filter pressure(P_(PRE))and transmembrane pressure(P_(TM))was performed.Receiver operating characteristic(ROC)curves were constructed to evaluate the accuracy of P_(TM) as an indicator for filter replacement.RESULTS:A total of 50 cases were analyzed,27 in the group A and 23 in the group B.Delivered doses of different molecular-weight solutes significantly decreased before changing the filter in both modalities,compared with those at the initiation of treatment(all P<0.05).In the late stage of CRRT,the possible rebound of serum medium-molecular-weight solute concentration was observed.P_(TM) was negatively correlated with the delivered dose of medium-molecular-weight solute in both modalities.The threshold for predicting the rebound of serum concentration of medium-molecularweight solute by P_(TM) was 146.5 mm Hg(1 mm Hg=0.133 k Pa).CONCLUSIONS:The filter can be used as long as possible within the manufacturer’s safe use time limits to remove small-molecular-weight solutes.P_(TM) of 146.5 mm Hg may be an optimal indicator for changing the filter in CRRT therapies to remove medium-molecular-weight solutes.
