[Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D. Antiqua. [Method] Full-length cDNA of ADH gene in D. antiqua was cloned by using RACE technology （GenBank access number： JQ66600...[Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D. Antiqua. [Method] Full-length cDNA of ADH gene in D. antiqua was cloned by using RACE technology （GenBank access number： JQ666006）. Analysis of the homology, characteristics and functional domains of ADH sequence and the phy- Iogenetic relationship to other dipteran ADH were conducted. [Result] The full length of ADH cDNA is 1 088 bp containing a 771 bp of ORF, encoding 256 amino acids, with a calculated relative molecular weight of 30.80 kDa and a theoretical isoelectric point of 8.22. The deduced amino acid sequence shares the highest homology with Glossina morsitans morsitans based on homological analysis and phylogenetic analysis. [Conclusion] This study provides basis for further research of ADH gene.展开更多
In the present study, we report the first complete mitochondrial genome （mitogenome） of the Painted Jezebel, Delias hyparete. The mitogenome ofDelias hyparete is 15 186 bp in length, and has typical sets of 37 genes...In the present study, we report the first complete mitochondrial genome （mitogenome） of the Painted Jezebel, Delias hyparete. The mitogenome ofDelias hyparete is 15 186 bp in length, and has typical sets of 37 genes： 13 protein-coding genes （PCGs）, 2 ribosomal RNAs, 22 transfer RNAs and a non-coding A＋T-rich region. All protein-coding genes are initiated by ATN codons, except for C01, which is tentatively designated by the CGA codon, as observed in other butterfly species. A total of 10 PCGs harbored the complete termination codon TAA or TAG; while the C01, C011and ND5 genes ended at a single T residue. All 22 tRNA genes show typical clover structures, with the exception of the tRNAser（AGn） which lacks the dihydrouridine （DHU） stem and is instead replaced by a simple loop. Thirteen intergenic spacers totaling 153 bp, and 13 overlapping regions totaling 46 bp are scattered throughout the whole genome. The 377 bp long olD. hyparete A＋T-rich region is not comprised of large repetitive sequences, but harbors several features characteristic of the lepidopteran insects, including the motif ATAGA followed by an 18 bp poly-T stretch, a microsatellite-like （AT）s element preceded by the ATTTA motif, an 10 bp polyA-like stretch （AAAAATAAAA） present immediately upstream tRNAMet.展开更多
In the present study,we report the first complete mitochondrial genome(mitogenome)of the Painted Jezebel,Delias hyparete.The mitogenome of Delias hyparete is 15186 bp in length,and has typical sets of 37 genes:13 prot...In the present study,we report the first complete mitochondrial genome(mitogenome)of the Painted Jezebel,Delias hyparete.The mitogenome of Delias hyparete is 15186 bp in length,and has typical sets of 37 genes:13 protein-coding genes(PCGs),2 ribosomal RNAs,22 transfer RNAs and a non-coding A+T-rich region.All protein-coding genes are initiated by ATN codons,except for COI,which is tentatively designated by the CGA codon,as observed in other butterfly species.A total of 10 PCGs harbored the complete termination codon TAA or TAG,while the COI,COII and ND5 genes ended at a single T residue.All 22 tRNA genes show typical clover structures,with the exception of the tRNASer(AGN)which lacks the dihydrouridine(DHU)stem and is instead replaced by a simple loop.Thirteen intergenic spacers totaling 153 bp,and 13 overlapping regions totaling 46 bp are scattered throughout the whole genome.The 377 bp long of D.hyparete A+T-rich region is not comprised of large repetitive sequences,but harbors several features characteristic of the lepidopteran insects,including the motif ATAGA followed by an 18 bp poly-T stretch,a microsatellite-like(AT)5 element preceded by the ATTTA motif,an 10 bp polyA-like stretch(AAAAATAAAA)present immediately upstream tRNAMet.展开更多
[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among...[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among deduced amino acid sequence of Dorsal cDNA and the Dorsals of other 14 insect species were compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-, summer- and non-diapausing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp, was obtained with ORF 1974 bp, which coded 657 amino acids with predicted Mw 72.9 kDa and PI 8.5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non- diapansing pupae, especially at the late diapanse, which might imply its relationship to D. antiqua diapause and development. [ Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.展开更多
【Aim】Insect diapause is a critical eco-physiological adaptation for seasonal survival,but little is known about the molecular mechanisms of summer and winter diapause of Delia antiqua.Here,our main goal is to identi...【Aim】Insect diapause is a critical eco-physiological adaptation for seasonal survival,but little is known about the molecular mechanisms of summer and winter diapause of Delia antiqua.Here,our main goal is to identify candidate genes linked to pupal diapause of D.antiqua.【Methods】A straightforward in silico approach was performed to predict the secreted diapause-related proteins based on the RNA-seq and digital gene expression datasets.Amino acid sequence similarity and basic physiochemical characteristics analysis were conducted.Gene expression levels of 12 selected genes from RNA-seq data were further validated in non-diapause pupae,and different developmental stages of summer and winter diapause pupae by qRT-PCR.【Results】A total of 38 putative secreted proteins were differentially regulated between the summer and winter diapause pupae of D.antiqua.Gene Ontology analysis revealed differentially expressed genes to be associated with odorant binding,chitin and lipid metabolic processes,innate immunity and development,etc.Among them,regulation of lipid mobilization and chitin-related metabolism was found to be important during diapause development.qRT-PCR analyses showed that the expression profiles of the 12 selected genes were in good agreement with results from RNA-seq(R=0.862,P=0.001).【Conclusion】This study establishes a robust pipeline for the discovery of diapause-related genes by secretome mining based on the high-throughput sequencing.展开更多
The full-length cDNA encoding Larval serum protein 2 (LSp-2) in the onion maggot,Delia antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends methods. The result showed that the cDNA was 2203 bp long and...The full-length cDNA encoding Larval serum protein 2 (LSp-2) in the onion maggot,Delia antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends methods. The result showed that the cDNA was 2203 bp long and the open reading frame (ORF) of 2106 bp encoded 701 amino acid with a calculated molecular weight of 80.5 kDa and an isoelectric point of 5.87. The onion maggot LSp-2 shows highest homology (83%) to that ofCalliphora vicinaat amino acid level. Its signal peptides, domains and structures were predicted and analyzed by using bioinformatic methods. The amino acid sequence of LSP-2 suggests that it would be a typical hexamerin.展开更多
The present paper deals with three new species of genus Delia from Gansu province, China, namely Delia ancylosurstyla sp. nov., D. apicifloralis sp. nov. and D. longiarista sp. nov.. All the type spec...The present paper deals with three new species of genus Delia from Gansu province, China, namely Delia ancylosurstyla sp. nov., D. apicifloralis sp. nov. and D. longiarista sp. nov.. All the type specimens are deposited in the Institute of Zoology, Chinese Academy of Sciences, Beijing , China.展开更多
The onion fly,Delia antiqua,is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons.However,little is known about its molecular regulation due to the lack of genomic res...The onion fly,Delia antiqua,is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons.However,little is known about its molecular regulation due to the lack of genomic resources.To gain insight into the possible mechanism of summer diapause(SD),high-throughput RNA-Seq data were generated from non-diapause(ND)and SD(initial,maintenance and quiescence phase)pupae.Three pair-wise comparisons were performed and identified,1380,1471 and 435,and were significantly regulated transcripts.Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation,cell cycle,carbon hydrate and lipid metabolism,innate immune and stress responses,various signalling transductions,ubiquitin-dependent proteosome,and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD.Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD.Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.展开更多
The present paper deals with two new species of the family Anthomyiidae from Yunnan Province,China,namely Delia nigriabdominis sp.nov.and Chirosia fortipispatula sp.nov.All the type specimens are deposited in Institut...The present paper deals with two new species of the family Anthomyiidae from Yunnan Province,China,namely Delia nigriabdominis sp.nov.and Chirosia fortipispatula sp.nov.All the type specimens are deposited in Institute of Entomology,Shenyang Normal University,Shenyang.展开更多
【目的】低分子量（12~43 k Da）热激蛋白（s HSPs）具有抗逆应答的功能,滞育是昆虫抵抗不良环境的特殊发育形式,但s HSPs在昆虫滞育发育过程中的作用仍不清楚。本研究克隆和特征化葱蝇Delia antiqua s HSP基因,并研究它在夏滞育和冬滞...【目的】低分子量（12~43 k Da）热激蛋白（s HSPs）具有抗逆应答的功能,滞育是昆虫抵抗不良环境的特殊发育形式,但s HSPs在昆虫滞育发育过程中的作用仍不清楚。本研究克隆和特征化葱蝇Delia antiqua s HSP基因,并研究它在夏滞育和冬滞育发育过程中的表达模式,为阐明s HSPs在滞育发育上的功能奠定基础。【方法】通过RACE-PCR方法克隆了葱蝇HSP23基因,通过相似性比较分析了其特征、结构域及与双翅目代表性同源基因的系统发育关系;采用实时荧光定量PCR研究了该基因在葱蝇冬滞育蛹和夏滞育蛹发育过程中的表达情况,通过表达的差异比较揭示了该基因与滞育发育的关系。【结果】克隆出了葱蝇HSP23基因,命名为Da HSP23（Gen Bank登录号：HQ392521.1）,其c DNA全长序列为904 bp,编码186个氨基酸,推测蛋白分子量为20.9 k Da,等电点为6.42。该基因的编码蛋白与其他双翅目昆虫的s HSPs有超过66%的氨基酸序列一致性,与已报道的其他双翅目昆虫的滞育相关HSP23基因同源。基因组测序显示该基因无内含子。Da HSP23基因在葱蝇非滞育蛹的发育过程中一直保持在较低的水平,各发育阶段间的表达量不存在显著差异。但在冬滞育和夏滞育蛹中,该基因从滞育起始期开始逐渐显著升高表达,到滞育维持期的中后期达到峰值,在滞育终止期逐渐降到较低的水平。【结论】Da HSP23基因在葱蝇冬滞育和夏滞育发育过程中明显上调表达,但存在差异,它在滞育期的调控可能是种专化的。Da HSP23可能在葱蝇两种类型的滞育上起重要作用。展开更多
基金Supported by National Natural Science Foundation of China (30870340,31071968)Scientific and Technological Research Project of Chongqing Municipal Education Commission (KJ100620)Key Project of Chongqing Normal University (2011XLZ12)~~
文摘[Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D. Antiqua. [Method] Full-length cDNA of ADH gene in D. antiqua was cloned by using RACE technology （GenBank access number： JQ666006）. Analysis of the homology, characteristics and functional domains of ADH sequence and the phy- Iogenetic relationship to other dipteran ADH were conducted. [Result] The full length of ADH cDNA is 1 088 bp containing a 771 bp of ORF, encoding 256 amino acids, with a calculated relative molecular weight of 30.80 kDa and a theoretical isoelectric point of 8.22. The deduced amino acid sequence shares the highest homology with Glossina morsitans morsitans based on homological analysis and phylogenetic analysis. [Conclusion] This study provides basis for further research of ADH gene.
基金Foundation items： This work was supported by the National Science Foundation of China （Grant No.41172004）, the CAS/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences （Grant No. KZCX22YW2JC104）, and the Opening Funds from the State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences （Grant No. 104143）
文摘In the present study, we report the first complete mitochondrial genome （mitogenome） of the Painted Jezebel, Delias hyparete. The mitogenome ofDelias hyparete is 15 186 bp in length, and has typical sets of 37 genes： 13 protein-coding genes （PCGs）, 2 ribosomal RNAs, 22 transfer RNAs and a non-coding A＋T-rich region. All protein-coding genes are initiated by ATN codons, except for C01, which is tentatively designated by the CGA codon, as observed in other butterfly species. A total of 10 PCGs harbored the complete termination codon TAA or TAG; while the C01, C011and ND5 genes ended at a single T residue. All 22 tRNA genes show typical clover structures, with the exception of the tRNAser（AGn） which lacks the dihydrouridine （DHU） stem and is instead replaced by a simple loop. Thirteen intergenic spacers totaling 153 bp, and 13 overlapping regions totaling 46 bp are scattered throughout the whole genome. The 377 bp long olD. hyparete A＋T-rich region is not comprised of large repetitive sequences, but harbors several features characteristic of the lepidopteran insects, including the motif ATAGA followed by an 18 bp poly-T stretch, a microsatellite-like （AT）s element preceded by the ATTTA motif, an 10 bp polyA-like stretch （AAAAATAAAA） present immediately upstream tRNAMet.
基金the National Science Foundation of China(Grant No.41172004)the CAS/SAFEA International Partnership Program for Creative Research Teams,Chinese Academy of Sciences(Grant No.KZCX22YW2JC104)the Opening Funds from the State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,Chinese Academy of Sciences(Grant No.104143)。
文摘In the present study,we report the first complete mitochondrial genome(mitogenome)of the Painted Jezebel,Delias hyparete.The mitogenome of Delias hyparete is 15186 bp in length,and has typical sets of 37 genes:13 protein-coding genes(PCGs),2 ribosomal RNAs,22 transfer RNAs and a non-coding A+T-rich region.All protein-coding genes are initiated by ATN codons,except for COI,which is tentatively designated by the CGA codon,as observed in other butterfly species.A total of 10 PCGs harbored the complete termination codon TAA or TAG,while the COI,COII and ND5 genes ended at a single T residue.All 22 tRNA genes show typical clover structures,with the exception of the tRNASer(AGN)which lacks the dihydrouridine(DHU)stem and is instead replaced by a simple loop.Thirteen intergenic spacers totaling 153 bp,and 13 overlapping regions totaling 46 bp are scattered throughout the whole genome.The 377 bp long of D.hyparete A+T-rich region is not comprised of large repetitive sequences,but harbors several features characteristic of the lepidopteran insects,including the motif ATAGA followed by an 18 bp poly-T stretch,a microsatellite-like(AT)5 element preceded by the ATTTA motif,an 10 bp polyA-like stretch(AAAAATAAAA)present immediately upstream tRNAMet.
基金Supported by National Natural Science Foundation of China (30870340,31071968)Science and Technology Research Projects of Chongqing Education Commission (KJ100620 )Key Project of School Fund of Chongqing Normal University (2011XLZ12)
文摘[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among deduced amino acid sequence of Dorsal cDNA and the Dorsals of other 14 insect species were compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-, summer- and non-diapausing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp, was obtained with ORF 1974 bp, which coded 657 amino acids with predicted Mw 72.9 kDa and PI 8.5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non- diapansing pupae, especially at the late diapanse, which might imply its relationship to D. antiqua diapause and development. [ Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.
文摘【Aim】Insect diapause is a critical eco-physiological adaptation for seasonal survival,but little is known about the molecular mechanisms of summer and winter diapause of Delia antiqua.Here,our main goal is to identify candidate genes linked to pupal diapause of D.antiqua.【Methods】A straightforward in silico approach was performed to predict the secreted diapause-related proteins based on the RNA-seq and digital gene expression datasets.Amino acid sequence similarity and basic physiochemical characteristics analysis were conducted.Gene expression levels of 12 selected genes from RNA-seq data were further validated in non-diapause pupae,and different developmental stages of summer and winter diapause pupae by qRT-PCR.【Results】A total of 38 putative secreted proteins were differentially regulated between the summer and winter diapause pupae of D.antiqua.Gene Ontology analysis revealed differentially expressed genes to be associated with odorant binding,chitin and lipid metabolic processes,innate immunity and development,etc.Among them,regulation of lipid mobilization and chitin-related metabolism was found to be important during diapause development.qRT-PCR analyses showed that the expression profiles of the 12 selected genes were in good agreement with results from RNA-seq(R=0.862,P=0.001).【Conclusion】This study establishes a robust pipeline for the discovery of diapause-related genes by secretome mining based on the high-throughput sequencing.
文摘The full-length cDNA encoding Larval serum protein 2 (LSp-2) in the onion maggot,Delia antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends methods. The result showed that the cDNA was 2203 bp long and the open reading frame (ORF) of 2106 bp encoded 701 amino acid with a calculated molecular weight of 80.5 kDa and an isoelectric point of 5.87. The onion maggot LSp-2 shows highest homology (83%) to that ofCalliphora vicinaat amino acid level. Its signal peptides, domains and structures were predicted and analyzed by using bioinformatic methods. The amino acid sequence of LSP-2 suggests that it would be a typical hexamerin.
文摘The present paper deals with three new species of genus Delia from Gansu province, China, namely Delia ancylosurstyla sp. nov., D. apicifloralis sp. nov. and D. longiarista sp. nov.. All the type specimens are deposited in the Institute of Zoology, Chinese Academy of Sciences, Beijing , China.
基金supported by Par-Eu Scholars Program,and The National Natural Science Foundation of China(31372265)Coordinated Research Project of the International Atomic Energy Agency(18268/R0)+1 种基金National Key Program of Science and Technology Foun-dation Work of China(2015FY210300).Conceived and designed the research:BC.Performed the experiments:FLS,DYE ZBHAnalyzed the data and wrote the paper:YJH,BC,YJZ.
文摘The onion fly,Delia antiqua,is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons.However,little is known about its molecular regulation due to the lack of genomic resources.To gain insight into the possible mechanism of summer diapause(SD),high-throughput RNA-Seq data were generated from non-diapause(ND)and SD(initial,maintenance and quiescence phase)pupae.Three pair-wise comparisons were performed and identified,1380,1471 and 435,and were significantly regulated transcripts.Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation,cell cycle,carbon hydrate and lipid metabolism,innate immune and stress responses,various signalling transductions,ubiquitin-dependent proteosome,and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD.Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD.Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.
文摘The present paper deals with two new species of the family Anthomyiidae from Yunnan Province,China,namely Delia nigriabdominis sp.nov.and Chirosia fortipispatula sp.nov.All the type specimens are deposited in Institute of Entomology,Shenyang Normal University,Shenyang.
文摘【目的】低分子量（12~43 k Da）热激蛋白（s HSPs）具有抗逆应答的功能,滞育是昆虫抵抗不良环境的特殊发育形式,但s HSPs在昆虫滞育发育过程中的作用仍不清楚。本研究克隆和特征化葱蝇Delia antiqua s HSP基因,并研究它在夏滞育和冬滞育发育过程中的表达模式,为阐明s HSPs在滞育发育上的功能奠定基础。【方法】通过RACE-PCR方法克隆了葱蝇HSP23基因,通过相似性比较分析了其特征、结构域及与双翅目代表性同源基因的系统发育关系;采用实时荧光定量PCR研究了该基因在葱蝇冬滞育蛹和夏滞育蛹发育过程中的表达情况,通过表达的差异比较揭示了该基因与滞育发育的关系。【结果】克隆出了葱蝇HSP23基因,命名为Da HSP23（Gen Bank登录号：HQ392521.1）,其c DNA全长序列为904 bp,编码186个氨基酸,推测蛋白分子量为20.9 k Da,等电点为6.42。该基因的编码蛋白与其他双翅目昆虫的s HSPs有超过66%的氨基酸序列一致性,与已报道的其他双翅目昆虫的滞育相关HSP23基因同源。基因组测序显示该基因无内含子。Da HSP23基因在葱蝇非滞育蛹的发育过程中一直保持在较低的水平,各发育阶段间的表达量不存在显著差异。但在冬滞育和夏滞育蛹中,该基因从滞育起始期开始逐渐显著升高表达,到滞育维持期的中后期达到峰值,在滞育终止期逐渐降到较低的水平。【结论】Da HSP23基因在葱蝇冬滞育和夏滞育发育过程中明显上调表达,但存在差异,它在滞育期的调控可能是种专化的。Da HSP23可能在葱蝇两种类型的滞育上起重要作用。
