Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects ...Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.展开更多
Background: The present study has evaluated the effects of different inclusion levels of a partially defatted black soldier fly(Hermetia illucens L.; HI) larva meal on the growth performance, blood parameters and gut ...Background: The present study has evaluated the effects of different inclusion levels of a partially defatted black soldier fly(Hermetia illucens L.; HI) larva meal on the growth performance, blood parameters and gut morphology of broiler chickens. A total of 256 male broiler chickens(Ross 308) were reared from d 1 to d 35 and assigned to4 dietary treatments(8 replicates/treatment and 8 birds/replicate). HI larva meal was included at increasing levels(0, 5%, 10% and 15%; HI0, HI5, HI10 and HI15, respectively) in isonitrogenous and isoenergetic diets formulated for 3 feeding phases: starter(1–10 d), growing(10–24 d) and finisher(24–35 d). Two birds per pen were slaughtered at d 35 and morphometric investigations and histopathological alterations were performed.Results: The live weight(LW) showed linear and quadratic responses to increasing HI larva meal(maximum for HI10 group). Average daily gain(ADG) showed a linear and quadratic responses to HI meal(maximum for HI10 group) during starter and growing periods. A linear decrease was observed for ADG during the finisher period. The daily feed intake(DFI) showed a linear and quadratic effect during the starter period(maximum for HI10 group).Linear and quadratic responses were observed for the feed conversion ratio(FCR) in the growing period and for the whole period of the experiment. The FCR showed a linear response in the finisher period(maximum for HI15).No significant effects were observed for the blood and serum parameters, except for the phosphorus concentration,which showed linear and quadratic responses as well as glutathione peroxidase(GPx) activity, the latter of which showed a linear response. The HI15 birds showed a lower villus height, a higher crypt depth and a lower villus height-to-crypt depth ratio than the other groups.Conclusions: Increasing levels of dietary HI meal inclusion in male broiler chickens may improve the LW and DFI during the starter period, but may also negatively affect the FCR and gut morphology, thus suggesting that low levels may be more suitable. However, no significant effects on the haematochemical parameters or histological findings were observed in relation to HI meal utilization.展开更多
Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain p...Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.展开更多
Defatted rice bran dietary fiber (DRBDF) was modified by micronization, ultrasound, microwave and extrusion cooking. We investigated the impacts of these physical treatments on the fermentation ability and bile salts ...Defatted rice bran dietary fiber (DRBDF) was modified by micronization, ultrasound, microwave and extrusion cooking. We investigated the impacts of these physical treatments on the fermentation ability and bile salts binding capacity of DRBDF. In-vitro fermentation by human fecal bacteria of modified fibers showed that the major fermentation products were propionic, acetate and butyrate acid. Fermentation of extruded fiber gave the highest amounts of propionic and acetic acid 135.76 and 25.45 mmol/L respectively, while, the fermented product with microwaved fiber had the highest butyric acid content (10.75 mmol/L). The amount of short-chain fatty acid increased from 12 h to 24 h and propionic acid was the predominant. On the other hand,in-vitrobile salts binding showed that extruded fiber had higher affinity with sodium deoxycholate and sodium chenodeoxycholate (66.14% and 30.25% respectively) while microwaved fiber exhibited the highest affinity with sodium taurocholate (14.38%). In the light of obtained results we can affirmed that these physical treatments significantly improved the fermentation products and bile salts binding capacity of DRBDF. Extrusion compared to the other physical treatment methods used in this study has greatly and positively influenced the fermentation and bile binding capacity of DRBDF.展开更多
The aim of this study was to optimize the conditions for the extraction of low-abundance proteins（LAPs） and the removal of abundant proteins（APs; β-conglycinin and glycinin） from soybean meal.Single factor and or...The aim of this study was to optimize the conditions for the extraction of low-abundance proteins（LAPs） and the removal of abundant proteins（APs; β-conglycinin and glycinin） from soybean meal.Single factor and orthogonal experiments were designed to determine the effects of four factors（isopropanol concentration, total extraction time, ultrasonic power, and ultrasonic time） on protein concentration in isopropanol extracts.Proteins in the isopropanol supernatant and the cold acetone precipitate of isopropanol were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） and matrix-assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF-MS）.The results showed that the optimal conditions were 50% isopropanol, ultrasonic pretreatment for 15 min at 350 W, and a total extraction time of 1 h.Under these conditions, the protein concentration in the isopropanol extracts reached 0.8081 g/L.Many LAPs were detected, including β-amylase, soybean agglutinin, soybean trypsin inhibitor, fumarylacetoacetase-like, phospholipase D alpha 1-like, oleosin, and even some unknown soybean proteins.The soybean APs（β-conglycinin and glycinin） were not found.The method may be useful for discovering new soybean proteins and extracting enough LAPs of soybean to allow further studies of their physiological effects on animals without the influence of APs.展开更多
Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked a...Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked and hydrolyzed media and growth turbidity of tested bacteria were determined.Results:Two percent defatted flour was found to be suitable concentration for the growth of pathogenic bacteria:Escherichia coli(ATCC 25922)(E,coli),Pseudomonas aeruginosa(ATCC27853),Salmonella(NCTC 8385)and Shigella flexneri(ATCC 12022)(S.flexneri),while 3%defatted flour was suitable for Staphylococcus aureus(ATCC 25923)(S.aureus).E.coli(93±1)and S.flexneri(524±1)colony count were significantly(P≤0.05)greater in defatted flour without supplement than in supplemented medium.E.coli[(3.72×10~9±2)CFU/mL],S.aureus[(7.4×10~9±2)CFU/mL],S.flexneri[(4.03×10~9±2)CFU/mL]and Salmonella[(2.37×10~9±1)CFU/mL]in non-hydrolyzed sample were statistically(P≤0.05)greater than hydrolyzed one and commercial peptone agar.Colony count of Salmonella[(4.55≤10~9±3)CFU/mL],S.flexneri[(5.40≤10~9±3)CFU/mL]and Lyesria moncytogenes(ATCC 19116)[(5.4×10~9±3)CFU/mL]on raw defatted flour agar was significantly(P≤0.05)greater than cooked defatted flour and commercial peptone agar.Biomass of E.coli,S.aureus.Salmonella and Enterococcus faecalis in non-hydrolyzed defatted flour is highly increased over hydrolyzed defatted flour and commercial peptone broth.Conclusions:The defatted flour agar was found to be better microbial media or comparable with peptone agar.The substances in it can serve as sources of carbon,nitrogen,vitamins and minerals that are essential to support the growth of microorganisms without any supplements.Currently,all supplements of peptone agar are very expensive in the market.展开更多
Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. ...Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield and content, such as extracting temperature, extracting time, the ratio of water to defatted kernel and concentration of ethanol were analyzed under specific condition. The optimal extracting parameters for ultrasound-associated extraction were determined as the ultrasonic temperature 70℃, the ratio of defatted kernel to water 1：20, the extracting time 40 min, and ethanol concentration 80%. Under such extraction conditions, the yield of water soluble polysaccharide was 3.65% and the average content of polysaccharide was 45.38% in the raw polysaccharides which gained in the experiment. Both extraction yield and content of polysaccharides extracted with ultrasound-associated extraction were higher than that with hot water extraction. The study demonstrates that ultrasound is a reliable and extremely effective tool for the fast extraction of water soluble polysaccharide of Korean pine kernel.展开更多
Background： This study was to determine if feeding laying hens with defatted diatom microalgal biomass （DFA） from biofuel production affected their egg production and health status. Methods： Five replicates of 5 i...Background： This study was to determine if feeding laying hens with defatted diatom microalgal biomass （DFA） from biofuel production affected their egg production and health status. Methods： Five replicates of 5 individually caged ISA Babcock White leghorn hens were fed 4 diets, including a corn-soybean meal control diet, a diet containing 7.5% DFA substituting for soybean meal, and diets containing 7.5% or 15% DFA substituting for corn and soybean meal. Body weights, feed intake, feed conversion ratio （FCR）, rate of egg production, egg size, egg mass, and several characteristics of eggs were determined at 4 and 8 wk. Venous blood was sampled at 4 and 8 wk for measurement of 5 biomarkers of health. Results： The15% DFA diet decreased （P 〈 0.05） feed intake, egg production, and plasma uric acid concentrations as compared with the control diet, but increased （P 〈 0.05） egg albumen weight and height compared with the 7.5% DFA diets. The two levels of DFA produced dose-dependent （P 〈 0.05） changes in three color measures of egg yolk, without affecting four hen plasma biochemical indicators of health. Conclusions： Feeding laying hens with 7.5% DFA in the corn-soybean meal diet for 8 wk had no adverse effect on their health, egg production, or egg quality, but 15% inclusion reduced feed intake, egg production, and efficiency of feed utilization.展开更多
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-...Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction.Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer(MCF-7 and MDA-MB-231), colon cancer(HT29), lung cancer(A549), cervical cancer(He La) and normal mouse fibroblast(3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope.Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction(P < 0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was(780.00 ± 28.28)and(895.00 ± 7.07) mg/m L, respectively. Cell shrinkage, membrane blebbing and formation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction.Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells.展开更多
In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational condit...In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational conditions using CO2 under pressurized conditions as solvent. Oil extractions were carried out in a laboratorial scale unit at temperatures ranging from 310 to 330 K, pressure from 15 to 25 MPa and solvent flow set in 3 mL min^-1. The experiment was conducted in a germination chamber （25℃, 12：12 h light：dark photoperiod）, and the germination rate and germination speed index （GSI） were evaluated for seven days. The results showed that the oil extraction with supercritical CO2 concentrates the allelochemical L-3,4-dihydroxyphenylalanine （L-DOPA） in bran with levels from 1.86% to 4.65%. Subsequent experiments revealed that the rate of soybean seed germination was not affected by defatted bran whereas the GSI increased significantly after treatment. In brief, we concluded that the extraction process with supercritical CO2 is efficient to obtain L-DOPA of Mucuna, which influences the soybean seed germination.展开更多
Cucumeropsis mannii, an underutilized oil seed was processed into raw full fat and defatted seed flours and its chemical, functional properties and anti-nutritional factors were determined using standard techniques. T...Cucumeropsis mannii, an underutilized oil seed was processed into raw full fat and defatted seed flours and its chemical, functional properties and anti-nutritional factors were determined using standard techniques. The effects of sample concentration and pH on the foaming properties of the seed flours were determined. The results showed that the full fat and defatted seed flours contained the following in g/100 g sample; 5.0 and 5.1; 45.8 and 1.0; 39.4 and 78.7; 3.45 and 4.40; 1.50 and 3.05; 4.85 and 7.75 for moisture, crude fat, protein, ash, crude fibre and carbohydrate, respectively. The most abundant mineral elements in the seed flour （mg/100 g） are potassium （198.5）, followed by nickel （30.0） and magnesium （28.4）. The water absorption capacity, oil absorption capacity, foaming capacity and stability, least gelation concentration, emulsion capacity and bulk density are 55.5% and 125.0%; 128.8% and 184.0%; 10.5% and 17.0%; 3.0% and 1.5%; 16.0% and 10.0 g/mL; 85.0 and 115.0 mL/g; 0.42 and 0.25 g/mL, respectively. Defatting influenced the functional properties. The foaming capacity is dependent on sample concentration and pH. The protein solubility of the full fat and defatted seed flours was minimum between pH 3 and 4 and maximum at pH 11. The anti-nutritional composition of the seed flour revealed the following： tannic acid, 1.54 mg/100 g; phytin phosphorus, 0.70 rag/g; phytic acid, 2.48 mg/g; oxalate, 1.85 mg/g; alkaloids 1.97% and saponin 0.50%. The seed flours （full fat and defatted） have potential as nutrient supplement, thickeners and emulsifiers in food system.展开更多
Glutamine peptides were obtained from defatted soybean meal by enzymatic hydrolysis using a combination of Protamex~? and trypsinase. The results showed that the extent of hydrolysis and the concentration of Gln pepti...Glutamine peptides were obtained from defatted soybean meal by enzymatic hydrolysis using a combination of Protamex~? and trypsinase. The results showed that the extent of hydrolysis and the concentration of Gln peptides in the hydrolysate were 22.02%and 6.05 mmol/L, respectively. The hydrolysates were fractionated by size-exclusion chromatography on a Sephadex G-15 into five major fractions(GelF1–GelF5). The peptide(GelF1) fraction with the highest glutamine peptide content(51.8%) was further evaluated to determine its molecular weight distribution. Most(92.37%) peptides were less than 1 000 Da. Glutamic acid and glutamine were the most abundant amino acids, accounting for up to 12.98% of the total amino acid content. In addition, the total amino acid content in GelF1 was higher than that in GelF2 and GelF3.展开更多
Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aime...Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aimed to clarify the profiles of phenolic compounds in the extracts from these by-products and their contribution to the anti-inflammatory activity.Twenty-one phenolic compounds were identified,belonging mainly to salicinoid analogues and p-coumaroyl derivatives.Idescarpin was the predominant phenolics in all extracts,especially in seed extract.Nearly half the p-coumaroyl derivatives presented relatively high content in the extracts of pulp and whole fruit.All extracts inhibited the production of nitric oxide(NO)and the mRNA expressions of inducible nitric oxide synthase(iNOS),tumor necrosis factor(TNF-α),and interleukin(IL)-6;and suppressed the accumulation of reactive oxygen species(ROS)along with the restoration of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.Seed extract exerted better potential than the pulp and whole fruit extracts.Furthermore,correlation analysis demonstrated that idescarpin and idesin were the crucial phenolics against inflammation,whereas some p-coumaroyl derivatives contributed to regulating oxidative stress.These findings suggested that phenolic extracts from defatted I.Polycarpa fruit and its parts,especially seed,could be utilized as inflammation inhibitors in the functional food and cosmetics industries.展开更多
Defatted rice bran(DRB)is a sustainable source of arabinoxylan(AX),a hemicellulose with significant prebiotic potential.However,the high viscosity and poor solubility of native AX often limit its industrial applicatio...Defatted rice bran(DRB)is a sustainable source of arabinoxylan(AX),a hemicellulose with significant prebiotic potential.However,the high viscosity and poor solubility of native AX often limit its industrial application.This study investigated the enzymatic conversion of DRB-AX into defatted rice bran arabinoxylan oligosaccharides(DRB-(A)XOS)using commercial endo-1,4-β-xylanase(Shearzyme®500L).Optimization of hydrolysis conditions revealed that 50 U/g substrate for 6 h achieved a maximal oligosaccharide yield of 92.13%,predominantly comprising xylobiose,xylotriose,and 32-α-L-arabinofuranosyl-xylobiose.In vitro digestion models simulating the upper gastrointestinal tract demonstrated that DRB-AX and DRB-(A)XOS are highly resistant to enzymatic hy-drolysis,with 77.80%and 74.72%of carbohydrates remaining undigested,respectively.Furthermore,in vitro fermentation using a human gut microbiome model highlighted the prebiotic efficacy of DRB-(A)XOS.While commercial xylooligosaccharides reduced microbial alpha-diversity,DRB-(A)XOS maintained community bal-ance while selectively enriching beneficial families,specifically Bacteroidaceae and Ruminococcaceae.Impor-tantly,the treatment significantly reduced the relative abundance of Coriobacteriaceae,a taxon associated with inflammation.Metabolic profiling revealed that DRB-(A)XOS fermentation significantly elevated total shortchain fatty acid(SCFA)production to 141.24μmol/mL,with acetate being the dominant metabolite(73.94%of total SCFAs).These results suggest that DRB-(A)XOS serves as a potent,digestion-resistant prebiotic ingredient that modulates specific gut taxa and enhances SCFA production,supporting its potential valorization in the functional food industry.展开更多
This study investigates the potential of defatted raspberry seed cake as a functional food ingredient and evaluates the polyphenol release using in vitro gastrointestinal digestion(GI)model.Techno-functional propertie...This study investigates the potential of defatted raspberry seed cake as a functional food ingredient and evaluates the polyphenol release using in vitro gastrointestinal digestion(GI)model.Techno-functional properties of the defatted raspberry seed cake included moisture content(9.55%),water holding capacity(1.78 g/g),oil holding capacity(1.22 g/g),swelling capacity(4.00 mL/g),and solvent retention capacity(162.6%,163.5%,198.2%and 189.1%for distilled water,5%lactic acid,5% sodium carbonate,and 50%sucrose,respectively).Polyphenol release was assessed by enzyme-based sequential digestion method and was highest in the oral and gastric phases,with flavan-3-ols(catechin and epicatechin)and hydroxybenzoic acids(gallic and ellagic acids)being predominant.Molecular docking studies showed a strong binding affinity of these polyphenols toα-amylase,suggesting potential effects on carbohydrate metabolism.Although our findings highlight favorable technofunctional and antioxidant properties,suggesting the potential of defatted raspberry seed cake in food applications,studies are needed to further evaluate its impact on the overall metabolic health.展开更多
Defatted egg yolk is a by-product of phospholipid extraction from egg yolk.The hydrolysate of defatted egg yolk(EYH)obtained in this study showed multi-functions such as osteogenic,metal-chelating,antioxidant and ACEi...Defatted egg yolk is a by-product of phospholipid extraction from egg yolk.The hydrolysate of defatted egg yolk(EYH)obtained in this study showed multi-functions such as osteogenic,metal-chelating,antioxidant and ACEinhibitory activities.This study aimed to provide a scalable method for simultaneously enriching and separating different bioactive peptides from EYH using macroporous resins.Static adsorption and desorption assays indi-cated that DA201-C as the optimal resin.The loading sample pH value adjusted the enrichment effects,and then different bioactive peptides were separated in different ethanol gradient elution.Loading sample pH 7.5 showed the best effects with a total recovery of 87.65%(w/w,based on EYH).W-1 fraction enriched osteogenic peptides with promoted MC3T3-E1 proliferation and differentiation,and metal-chelating activities,Ethl-20%fraction enriched antioxidant peptides(TEAC=675.63μmol TE/g),Ethl-40%and Ethl-80%fractions enriched ACEinhibitory peptides(IC_(50)=0.26 mg/mL).More than twenty phosphopeptides were identified in W-1 by LC-MS/MS,which contributed to osteogenic activity.This research offered a simple and effective technique for large-scale enriching and separating osteogenic,metal-chelating,antioxidant,and ACE-inhibitory peptides from EYH,which is beneficial for the full utilization of defatted egg yolk powder.展开更多
In this study,the supercritical carbon dioxide(SC-CO_(2))defatted spirulina(SC-D-Sp)(25.0 MPa,50.0℃,16.0 mL/min,and 60.0 min)was used as a substrate to promote lactic acid bacteria(LAB)growth(5%Lactobacillus plantaru...In this study,the supercritical carbon dioxide(SC-CO_(2))defatted spirulina(SC-D-Sp)(25.0 MPa,50.0℃,16.0 mL/min,and 60.0 min)was used as a substrate to promote lactic acid bacteria(LAB)growth(5%Lactobacillus plantarum(LP)or 5%Lactobacillus casei(LC))as the sole carbon source.Moreover,pulsed electric field(PEF)treatment(3 kV/cm,44 pulses,and 99 kJ/kg)was used to promote LAB fermentation.The fermented solids and supernatants were then collected by centrifugation(1409 g force in 30 min),and ultrafiltration(membrane size of 10 kDa)was applied to obtain the liquid fraction(<10 kDa and>10 kDa).The higher protein content was found in solid matrices compared to the liquid fraction.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)allowed the identification of 7 types of bioactive peptide sequences(antioxidant,antithrombotic,hypolipidemic,anticancer,anti-inflammatory,calcium-binding,and zinc-binding).As for inductively coupled plasma-mass spectrometry(ICP-MS)of mineral determination,the liquid fraction>10 kDa presented the highest values for all the Mg,P,K,Ca,and Fe.Moreover,the liquid fraction>10 kDa contained higher total antioxidant capacity(TAC).In conclusion,applying SC-D-Sp via PEF-assisted and ultrafiltration could promote the LAB fermentation,and more bioactive sequences with various activities,various mineral distribution,and higher antioxidant compounds.It could lay the groundwork for future work on how these steps affect the in vitro digestion and the health benefits of the resulting bioactive compounds.展开更多
Based on preparation of co-fermented defatted rice bran(DFRB)using Bacillus subtilis,Saccharomyces cerevisiae,Lactobacillus plantarum and phytase,the present study aimed to evaluate the effects of cofermented DFRB on ...Based on preparation of co-fermented defatted rice bran(DFRB)using Bacillus subtilis,Saccharomyces cerevisiae,Lactobacillus plantarum and phytase,the present study aimed to evaluate the effects of cofermented DFRB on growth performance,antioxidant capacity,immune status,gut microbiota and permeability in finishing pigs.Ninety finishing pigs(85.30±0.97 kg)were randomly assigned to 3treatments(3 replicates/treatment)with a basal diet(Ctrl),a basal diet supplemented with 10% unfermented DFRB(UFR),and a basal diet supplemented with 10% fermented DFRB(FR)for 30 d.Results revealed that the diet supplemented with FR notably(P<0.05)improved the average daily gain(ADG),gain to feed ratio(G:F)and the digestibility of crude protein,amino acids and dietary fiber of finishing pigs compared with UFR.Additionally,FR supplementation significantly(P<0.05)increased total antioxidant capacity,the activities of superoxide dismutase and catalase,and decreased the content of malonaldehyde in serum.Furthermore,FR remarkably(P<0.05)increased serum levels of Ig G,antiinflammatory cytokines(IL-22 and IL-23)and reduced pro-inflammatory cytokines(TNF-α,IL-1β and INF-γ).The decrease of serum diamine oxidase activity and serum D-lactate content in the FR group(P<0.05)suggested an improvement in intestinal permeability.Supplementation of FR also elevated the content of acetate and butyrate in feces(P<0.05).Moreover,FR enhanced gut microbial richness and the abundance of fiber-degrading bacteria such as Clostridium butyricum and Lactobacillus amylovorus.Correlation analyses indicated dietary fiber in FR was associated with improvements in immune status,intestinal permeability and the level of butyrate-producing microbe C.butyricum,which was also verified by the in vitro fermentation analysis.These findings provided an experimental and theoretical basis for the application of fermented DFRB in finishing pigs.展开更多
Defatted chickpea flour(DCF),which is a by-product of chickpea oil extraction industry,is rich in nutrients that are beneficial to human health.In this study,the effects of temperature and DCF variation on the rheolog...Defatted chickpea flour(DCF),which is a by-product of chickpea oil extraction industry,is rich in nutrients that are beneficial to human health.In this study,the effects of temperature and DCF variation on the rheological properties,water holding capacity,freeze-thaw stability and microstructure of DCF heat induced gels were investigated.The results showed that the viscoelasticity,frequency dependence,and resistance strength of heat induced gels increased significantly with the increase of temperature and DCF variation.The degree of denaturation and water retention of heat induced gels increased significantly with increased variables within the temperature and variation windows of 75℃ to 95℃ and 13%to 21%.The CLSM results revealed that variations of both temperature and DCF variation could cause the proteins in the heat induced gels to aggregate gradually and to form protein aggregations.When temperature or variation exceeded certain value(85℃ or 17%),the protein aggregations broke up and the protein clusters became smaller and more homogeneous.Therefore,the heat induced gels presented better water holding capacity,viscoelasticity,structural stability and gel property at a temperature of 95℃ or a DCF variation of 21%within the present experimental range.展开更多
Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibil...Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibility and fatty acid(FA)biohydrogenation is scant.This trial aims to investigate the effects(i)of drying temperature of fullfat Hermetia illucens(HI)and Tenebrio molitor(TM)meals,and(ii)of residual ether extract(EE)content of defatted HI and TM meals,on their fermentation characteristics and FA of rumen digesta after 24-h in vitro rumen incubation.Methods The tested full-fat meals included four HI and four TM meals obtained applying drying temperatures ranging from 30℃ to 70℃,while the tested defatted meals consisted of five HI and two TM meals containing a residual EE content ranging from 4.7 to 19.7 g EE/100 g dry matter(DM).The applied statistical models(GLM ANOVA)tested the effects of insect species,drying temperature(full-fat meals)or EE content(defatted meals),and their interaction.Results Drying temperature had minor effects on in vitro ruminal digestibility and FA profile of rumen digesta.Irrespective of insect species,increasing the drying temperature led to a reduction of in vitro degradation of proteins from insect meals,as outlined by the significant decrease in ammonia production(-0.009 mmol/g DM and-0.126 g/100 g total N for each additional 1℃).Irrespective of insect species,defatting increased total gas,volatile fatty acids(VFA)and CH_(4) productions,and the proportions of total saturated and branched-chain FA in rumen digesta(+0.038 mmol/g DM,+0.063 mmol/g DM,+12.9μmol/g DM,+0.18 g/100 g FA,and+0.19 g/100 g FA for each reduced 1 g EE/100 g DM,respectively),and reduced the proportion of total PUFA(-0.12 g/100 g FA).Conclusions The applied drying temperatures of full-fat insect meals are too low to exert impactful effects on rumen digestibility and FA biohydrogenation.Fat lowered fermentation activity,probably because of an inhibitory effect on rumen microbiota.The increased ruminal digestibility of defatted insect meals suggests that they can be more suitable to be used in ruminant nutrition than full-fat ones.展开更多
基金supported by grants from the National Key R&D Program(2023YFD1301303)National Natural Science Foundation of China(32472950,U21A20249)+1 种基金China Agriculture Research System of MOF and MARA(CARS-35)National Center of Technology Innovation for Pigs,Zhejiang Agricultural Talents,Taishan Industrial Leading Talents Project.
文摘Emerging evidence of the beneficial effects of defatted rice bran(DFRB)on gut health has advanced the development of fermented defatted rice bran as a potential functional food.However,less is known about its effects and underlying mechanisms on gut health.In this study,a mouse model together with fecal microbiota transplantation(FMT)was utilized to study the effects and mechanisms of fermented DFRB(FR)on gut barrier function.We found that FR improved the intestinal morphology,gut tight junction proteins,mucin,antimicrobial peptides,and interleukin 22(IL-22)and promoted the gut Clostridium butyricum and butyrate.Notably,correlation analysis indicated gut C.butyricum and butyrate were two FR-induced effectors that improved gut health.FMT results suggested that C.butyricum,butyrate,and fecal microbiota from the FR group all reduced prolyl hydroxylase 2(PHD2)expression by activating peroxisome proliferator-activated receptor gamma(PPARγ)in the mouse colon.This decrease in gut PHD2 subsequently upregulated the hypoxia-inducible factor-1 alpha(HIF-1α)expression,which in turn increased the expression of its targeted downstream tight junction proteins,mucin and antimicrobial peptides,and colonic IL-22 secretion.Overall,FR-derived C.butyricum and butyrate might improve gut barrier function through the HIF-1 signaling pathway,which provides a reference for the application of fermented DFRB as a potential functional food for improving of gut barrier function.
基金supported by University of Torino(Italy)founding:SCHA_RILO_14_01(2015–2017)and SCHA_RILO_16_02(2016–2018)
文摘Background: The present study has evaluated the effects of different inclusion levels of a partially defatted black soldier fly(Hermetia illucens L.; HI) larva meal on the growth performance, blood parameters and gut morphology of broiler chickens. A total of 256 male broiler chickens(Ross 308) were reared from d 1 to d 35 and assigned to4 dietary treatments(8 replicates/treatment and 8 birds/replicate). HI larva meal was included at increasing levels(0, 5%, 10% and 15%; HI0, HI5, HI10 and HI15, respectively) in isonitrogenous and isoenergetic diets formulated for 3 feeding phases: starter(1–10 d), growing(10–24 d) and finisher(24–35 d). Two birds per pen were slaughtered at d 35 and morphometric investigations and histopathological alterations were performed.Results: The live weight(LW) showed linear and quadratic responses to increasing HI larva meal(maximum for HI10 group). Average daily gain(ADG) showed a linear and quadratic responses to HI meal(maximum for HI10 group) during starter and growing periods. A linear decrease was observed for ADG during the finisher period. The daily feed intake(DFI) showed a linear and quadratic effect during the starter period(maximum for HI10 group).Linear and quadratic responses were observed for the feed conversion ratio(FCR) in the growing period and for the whole period of the experiment. The FCR showed a linear response in the finisher period(maximum for HI15).No significant effects were observed for the blood and serum parameters, except for the phosphorus concentration,which showed linear and quadratic responses as well as glutathione peroxidase(GPx) activity, the latter of which showed a linear response. The HI15 birds showed a lower villus height, a higher crypt depth and a lower villus height-to-crypt depth ratio than the other groups.Conclusions: Increasing levels of dietary HI meal inclusion in male broiler chickens may improve the LW and DFI during the starter period, but may also negatively affect the FCR and gut morphology, thus suggesting that low levels may be more suitable. However, no significant effects on the haematochemical parameters or histological findings were observed in relation to HI meal utilization.
基金supported by the National Natural Science Foundation of China (No.30871943)the National Natural Science Foundation of China (No.3080 0858)
文摘Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.
文摘Defatted rice bran dietary fiber (DRBDF) was modified by micronization, ultrasound, microwave and extrusion cooking. We investigated the impacts of these physical treatments on the fermentation ability and bile salts binding capacity of DRBDF. In-vitro fermentation by human fecal bacteria of modified fibers showed that the major fermentation products were propionic, acetate and butyrate acid. Fermentation of extruded fiber gave the highest amounts of propionic and acetic acid 135.76 and 25.45 mmol/L respectively, while, the fermented product with microwaved fiber had the highest butyric acid content (10.75 mmol/L). The amount of short-chain fatty acid increased from 12 h to 24 h and propionic acid was the predominant. On the other hand,in-vitrobile salts binding showed that extruded fiber had higher affinity with sodium deoxycholate and sodium chenodeoxycholate (66.14% and 30.25% respectively) while microwaved fiber exhibited the highest affinity with sodium taurocholate (14.38%). In the light of obtained results we can affirmed that these physical treatments significantly improved the fermentation products and bile salts binding capacity of DRBDF. Extrusion compared to the other physical treatment methods used in this study has greatly and positively influenced the fermentation and bile binding capacity of DRBDF.
基金Project supported by the China Agriculture Research System(No.CARS-36)the National Natural Science Foundation of China(No.31572430)
文摘The aim of this study was to optimize the conditions for the extraction of low-abundance proteins（LAPs） and the removal of abundant proteins（APs; β-conglycinin and glycinin） from soybean meal.Single factor and orthogonal experiments were designed to determine the effects of four factors（isopropanol concentration, total extraction time, ultrasonic power, and ultrasonic time） on protein concentration in isopropanol extracts.Proteins in the isopropanol supernatant and the cold acetone precipitate of isopropanol were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） and matrix-assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF-MS）.The results showed that the optimal conditions were 50% isopropanol, ultrasonic pretreatment for 15 min at 350 W, and a total extraction time of 1 h.Under these conditions, the protein concentration in the isopropanol extracts reached 0.8081 g/L.Many LAPs were detected, including β-amylase, soybean agglutinin, soybean trypsin inhibitor, fumarylacetoacetase-like, phospholipase D alpha 1-like, oleosin, and even some unknown soybean proteins.The soybean APs（β-conglycinin and glycinin） were not found.The method may be useful for discovering new soybean proteins and extracting enough LAPs of soybean to allow further studies of their physiological effects on animals without the influence of APs.
基金supported by the University of Gondar:General Director.Research & Community Service Core Process,Grant NO:UOG/Budget/no.6215
文摘Objective:To investigate and optimize microbial media that substitute peptone agar using brebra seed defatted flour.Methods:Defatted process.inoculums preparation,evaluation of bacterial growth,preparation of cooked and hydrolyzed media and growth turbidity of tested bacteria were determined.Results:Two percent defatted flour was found to be suitable concentration for the growth of pathogenic bacteria:Escherichia coli(ATCC 25922)(E,coli),Pseudomonas aeruginosa(ATCC27853),Salmonella(NCTC 8385)and Shigella flexneri(ATCC 12022)(S.flexneri),while 3%defatted flour was suitable for Staphylococcus aureus(ATCC 25923)(S.aureus).E.coli(93±1)and S.flexneri(524±1)colony count were significantly(P≤0.05)greater in defatted flour without supplement than in supplemented medium.E.coli[(3.72×10~9±2)CFU/mL],S.aureus[(7.4×10~9±2)CFU/mL],S.flexneri[(4.03×10~9±2)CFU/mL]and Salmonella[(2.37×10~9±1)CFU/mL]in non-hydrolyzed sample were statistically(P≤0.05)greater than hydrolyzed one and commercial peptone agar.Colony count of Salmonella[(4.55≤10~9±3)CFU/mL],S.flexneri[(5.40≤10~9±3)CFU/mL]and Lyesria moncytogenes(ATCC 19116)[(5.4×10~9±3)CFU/mL]on raw defatted flour agar was significantly(P≤0.05)greater than cooked defatted flour and commercial peptone agar.Biomass of E.coli,S.aureus.Salmonella and Enterococcus faecalis in non-hydrolyzed defatted flour is highly increased over hydrolyzed defatted flour and commercial peptone broth.Conclusions:The defatted flour agar was found to be better microbial media or comparable with peptone agar.The substances in it can serve as sources of carbon,nitrogen,vitamins and minerals that are essential to support the growth of microorganisms without any supplements.Currently,all supplements of peptone agar are very expensive in the market.
文摘Experiments of ultrasonic-associated extraction and hot water extraction of water soluble polysaccharides from defatted Korean pine （Pinus koraiensis） were conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield and content, such as extracting temperature, extracting time, the ratio of water to defatted kernel and concentration of ethanol were analyzed under specific condition. The optimal extracting parameters for ultrasound-associated extraction were determined as the ultrasonic temperature 70℃, the ratio of defatted kernel to water 1：20, the extracting time 40 min, and ethanol concentration 80%. Under such extraction conditions, the yield of water soluble polysaccharide was 3.65% and the average content of polysaccharide was 45.38% in the raw polysaccharides which gained in the experiment. Both extraction yield and content of polysaccharides extracted with ultrasound-associated extraction were higher than that with hot water extraction. The study demonstrates that ultrasound is a reliable and extremely effective tool for the fast extraction of water soluble polysaccharide of Korean pine kernel.
基金supported in part by a USDA/DOE Biomass R&D Initiative grant
文摘Background： This study was to determine if feeding laying hens with defatted diatom microalgal biomass （DFA） from biofuel production affected their egg production and health status. Methods： Five replicates of 5 individually caged ISA Babcock White leghorn hens were fed 4 diets, including a corn-soybean meal control diet, a diet containing 7.5% DFA substituting for soybean meal, and diets containing 7.5% or 15% DFA substituting for corn and soybean meal. Body weights, feed intake, feed conversion ratio （FCR）, rate of egg production, egg size, egg mass, and several characteristics of eggs were determined at 4 and 8 wk. Venous blood was sampled at 4 and 8 wk for measurement of 5 biomarkers of health. Results： The15% DFA diet decreased （P 〈 0.05） feed intake, egg production, and plasma uric acid concentrations as compared with the control diet, but increased （P 〈 0.05） egg albumen weight and height compared with the 7.5% DFA diets. The two levels of DFA produced dose-dependent （P 〈 0.05） changes in three color measures of egg yolk, without affecting four hen plasma biochemical indicators of health. Conclusions： Feeding laying hens with 7.5% DFA in the corn-soybean meal diet for 8 wk had no adverse effect on their health, egg production, or egg quality, but 15% inclusion reduced feed intake, egg production, and efficiency of feed utilization.
基金Supported by Fundamental Research Grant Scheme and Research University Grant Scheme[RUGS 7(9359500)]
文摘Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction.Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer(MCF-7 and MDA-MB-231), colon cancer(HT29), lung cancer(A549), cervical cancer(He La) and normal mouse fibroblast(3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope.Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction(P < 0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was(780.00 ± 28.28)and(895.00 ± 7.07) mg/m L, respectively. Cell shrinkage, membrane blebbing and formation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction.Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells.
文摘In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational conditions using CO2 under pressurized conditions as solvent. Oil extractions were carried out in a laboratorial scale unit at temperatures ranging from 310 to 330 K, pressure from 15 to 25 MPa and solvent flow set in 3 mL min^-1. The experiment was conducted in a germination chamber （25℃, 12：12 h light：dark photoperiod）, and the germination rate and germination speed index （GSI） were evaluated for seven days. The results showed that the oil extraction with supercritical CO2 concentrates the allelochemical L-3,4-dihydroxyphenylalanine （L-DOPA） in bran with levels from 1.86% to 4.65%. Subsequent experiments revealed that the rate of soybean seed germination was not affected by defatted bran whereas the GSI increased significantly after treatment. In brief, we concluded that the extraction process with supercritical CO2 is efficient to obtain L-DOPA of Mucuna, which influences the soybean seed germination.
文摘Cucumeropsis mannii, an underutilized oil seed was processed into raw full fat and defatted seed flours and its chemical, functional properties and anti-nutritional factors were determined using standard techniques. The effects of sample concentration and pH on the foaming properties of the seed flours were determined. The results showed that the full fat and defatted seed flours contained the following in g/100 g sample; 5.0 and 5.1; 45.8 and 1.0; 39.4 and 78.7; 3.45 and 4.40; 1.50 and 3.05; 4.85 and 7.75 for moisture, crude fat, protein, ash, crude fibre and carbohydrate, respectively. The most abundant mineral elements in the seed flour （mg/100 g） are potassium （198.5）, followed by nickel （30.0） and magnesium （28.4）. The water absorption capacity, oil absorption capacity, foaming capacity and stability, least gelation concentration, emulsion capacity and bulk density are 55.5% and 125.0%; 128.8% and 184.0%; 10.5% and 17.0%; 3.0% and 1.5%; 16.0% and 10.0 g/mL; 85.0 and 115.0 mL/g; 0.42 and 0.25 g/mL, respectively. Defatting influenced the functional properties. The foaming capacity is dependent on sample concentration and pH. The protein solubility of the full fat and defatted seed flours was minimum between pH 3 and 4 and maximum at pH 11. The anti-nutritional composition of the seed flour revealed the following： tannic acid, 1.54 mg/100 g; phytin phosphorus, 0.70 rag/g; phytic acid, 2.48 mg/g; oxalate, 1.85 mg/g; alkaloids 1.97% and saponin 0.50%. The seed flours （full fat and defatted） have potential as nutrient supplement, thickeners and emulsifiers in food system.
基金Supported by National Key R&D Program of China(2016YFD0400203)
文摘Glutamine peptides were obtained from defatted soybean meal by enzymatic hydrolysis using a combination of Protamex~? and trypsinase. The results showed that the extent of hydrolysis and the concentration of Gln peptides in the hydrolysate were 22.02%and 6.05 mmol/L, respectively. The hydrolysates were fractionated by size-exclusion chromatography on a Sephadex G-15 into five major fractions(GelF1–GelF5). The peptide(GelF1) fraction with the highest glutamine peptide content(51.8%) was further evaluated to determine its molecular weight distribution. Most(92.37%) peptides were less than 1 000 Da. Glutamic acid and glutamine were the most abundant amino acids, accounting for up to 12.98% of the total amino acid content. In addition, the total amino acid content in GelF1 was higher than that in GelF2 and GelF3.
基金sincerely appreciate the support provided by the Na-tional Natural Science Foundation of China(32272242)Zhejiang Province Key Research and Development Projects(2024C04014,2025C04041)Zhejiang Provincial Natural Science Foundation of China(LTGN24C200011).
文摘Defatted seed,pulp and whole fruit of Idesia Polycarpa Maxin.(I.Polycarpa)are the by-products of the oil extraction process,but have attracted little attention as natural sources of bioactive phenolics.This study aimed to clarify the profiles of phenolic compounds in the extracts from these by-products and their contribution to the anti-inflammatory activity.Twenty-one phenolic compounds were identified,belonging mainly to salicinoid analogues and p-coumaroyl derivatives.Idescarpin was the predominant phenolics in all extracts,especially in seed extract.Nearly half the p-coumaroyl derivatives presented relatively high content in the extracts of pulp and whole fruit.All extracts inhibited the production of nitric oxide(NO)and the mRNA expressions of inducible nitric oxide synthase(iNOS),tumor necrosis factor(TNF-α),and interleukin(IL)-6;and suppressed the accumulation of reactive oxygen species(ROS)along with the restoration of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.Seed extract exerted better potential than the pulp and whole fruit extracts.Furthermore,correlation analysis demonstrated that idescarpin and idesin were the crucial phenolics against inflammation,whereas some p-coumaroyl derivatives contributed to regulating oxidative stress.These findings suggested that phenolic extracts from defatted I.Polycarpa fruit and its parts,especially seed,could be utilized as inflammation inhibitors in the functional food and cosmetics industries.
基金funded by Research and Researchers for Industries(RRi)Ph.D.scholarship(Code:PHD60I0077)awarded by The National Research Council of Thailand(NRCT)supported by Chiang Mai University and Cluster of High Value Products from Thai Rice and Plants for Health,Chiang Mai University,Thailand.
文摘Defatted rice bran(DRB)is a sustainable source of arabinoxylan(AX),a hemicellulose with significant prebiotic potential.However,the high viscosity and poor solubility of native AX often limit its industrial application.This study investigated the enzymatic conversion of DRB-AX into defatted rice bran arabinoxylan oligosaccharides(DRB-(A)XOS)using commercial endo-1,4-β-xylanase(Shearzyme®500L).Optimization of hydrolysis conditions revealed that 50 U/g substrate for 6 h achieved a maximal oligosaccharide yield of 92.13%,predominantly comprising xylobiose,xylotriose,and 32-α-L-arabinofuranosyl-xylobiose.In vitro digestion models simulating the upper gastrointestinal tract demonstrated that DRB-AX and DRB-(A)XOS are highly resistant to enzymatic hy-drolysis,with 77.80%and 74.72%of carbohydrates remaining undigested,respectively.Furthermore,in vitro fermentation using a human gut microbiome model highlighted the prebiotic efficacy of DRB-(A)XOS.While commercial xylooligosaccharides reduced microbial alpha-diversity,DRB-(A)XOS maintained community bal-ance while selectively enriching beneficial families,specifically Bacteroidaceae and Ruminococcaceae.Impor-tantly,the treatment significantly reduced the relative abundance of Coriobacteriaceae,a taxon associated with inflammation.Metabolic profiling revealed that DRB-(A)XOS fermentation significantly elevated total shortchain fatty acid(SCFA)production to 141.24μmol/mL,with acetate being the dominant metabolite(73.94%of total SCFAs).These results suggest that DRB-(A)XOS serves as a potent,digestion-resistant prebiotic ingredient that modulates specific gut taxa and enhances SCFA production,supporting its potential valorization in the functional food industry.
基金the Ministry of Science,Technological Development and Innovation of the Republic of Serbia(451-03-136/2025-03/200288,451-03-136/2025-03/200168 and 451-03-137/2025-03/200116)。
文摘This study investigates the potential of defatted raspberry seed cake as a functional food ingredient and evaluates the polyphenol release using in vitro gastrointestinal digestion(GI)model.Techno-functional properties of the defatted raspberry seed cake included moisture content(9.55%),water holding capacity(1.78 g/g),oil holding capacity(1.22 g/g),swelling capacity(4.00 mL/g),and solvent retention capacity(162.6%,163.5%,198.2%and 189.1%for distilled water,5%lactic acid,5% sodium carbonate,and 50%sucrose,respectively).Polyphenol release was assessed by enzyme-based sequential digestion method and was highest in the oral and gastric phases,with flavan-3-ols(catechin and epicatechin)and hydroxybenzoic acids(gallic and ellagic acids)being predominant.Molecular docking studies showed a strong binding affinity of these polyphenols toα-amylase,suggesting potential effects on carbohydrate metabolism.Although our findings highlight favorable technofunctional and antioxidant properties,suggesting the potential of defatted raspberry seed cake in food applications,studies are needed to further evaluate its impact on the overall metabolic health.
基金supported by the National Key Research and Development Program of China[2022YFD2101003].
文摘Defatted egg yolk is a by-product of phospholipid extraction from egg yolk.The hydrolysate of defatted egg yolk(EYH)obtained in this study showed multi-functions such as osteogenic,metal-chelating,antioxidant and ACEinhibitory activities.This study aimed to provide a scalable method for simultaneously enriching and separating different bioactive peptides from EYH using macroporous resins.Static adsorption and desorption assays indi-cated that DA201-C as the optimal resin.The loading sample pH value adjusted the enrichment effects,and then different bioactive peptides were separated in different ethanol gradient elution.Loading sample pH 7.5 showed the best effects with a total recovery of 87.65%(w/w,based on EYH).W-1 fraction enriched osteogenic peptides with promoted MC3T3-E1 proliferation and differentiation,and metal-chelating activities,Ethl-20%fraction enriched antioxidant peptides(TEAC=675.63μmol TE/g),Ethl-40%and Ethl-80%fractions enriched ACEinhibitory peptides(IC_(50)=0.26 mg/mL).More than twenty phosphopeptides were identified in W-1 by LC-MS/MS,which contributed to osteogenic activity.This research offered a simple and effective technique for large-scale enriching and separating osteogenic,metal-chelating,antioxidant,and ACE-inhibitory peptides from EYH,which is beneficial for the full utilization of defatted egg yolk powder.
基金The publication is part of the BLUEWAYSE"BLUE WAY to a Sus-tainable Europe"action PCI2024-153460(Sustainable Blue Economy Partnership,SBEP),funded by MICIU/AEI/10.13039/501100011033 and by the European Union under grant agreement n◦:SBEP2023-961.
文摘In this study,the supercritical carbon dioxide(SC-CO_(2))defatted spirulina(SC-D-Sp)(25.0 MPa,50.0℃,16.0 mL/min,and 60.0 min)was used as a substrate to promote lactic acid bacteria(LAB)growth(5%Lactobacillus plantarum(LP)or 5%Lactobacillus casei(LC))as the sole carbon source.Moreover,pulsed electric field(PEF)treatment(3 kV/cm,44 pulses,and 99 kJ/kg)was used to promote LAB fermentation.The fermented solids and supernatants were then collected by centrifugation(1409 g force in 30 min),and ultrafiltration(membrane size of 10 kDa)was applied to obtain the liquid fraction(<10 kDa and>10 kDa).The higher protein content was found in solid matrices compared to the liquid fraction.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)allowed the identification of 7 types of bioactive peptide sequences(antioxidant,antithrombotic,hypolipidemic,anticancer,anti-inflammatory,calcium-binding,and zinc-binding).As for inductively coupled plasma-mass spectrometry(ICP-MS)of mineral determination,the liquid fraction>10 kDa presented the highest values for all the Mg,P,K,Ca,and Fe.Moreover,the liquid fraction>10 kDa contained higher total antioxidant capacity(TAC).In conclusion,applying SC-D-Sp via PEF-assisted and ultrafiltration could promote the LAB fermentation,and more bioactive sequences with various activities,various mineral distribution,and higher antioxidant compounds.It could lay the groundwork for future work on how these steps affect the in vitro digestion and the health benefits of the resulting bioactive compounds.
基金Science and Technology Projects of Zhejiang(2022C02043,2021C02008,CTZB-2020080127)China Agriculture Research System(CARS-35)National Center of Technology Innovation for Pigs。
文摘Based on preparation of co-fermented defatted rice bran(DFRB)using Bacillus subtilis,Saccharomyces cerevisiae,Lactobacillus plantarum and phytase,the present study aimed to evaluate the effects of cofermented DFRB on growth performance,antioxidant capacity,immune status,gut microbiota and permeability in finishing pigs.Ninety finishing pigs(85.30±0.97 kg)were randomly assigned to 3treatments(3 replicates/treatment)with a basal diet(Ctrl),a basal diet supplemented with 10% unfermented DFRB(UFR),and a basal diet supplemented with 10% fermented DFRB(FR)for 30 d.Results revealed that the diet supplemented with FR notably(P<0.05)improved the average daily gain(ADG),gain to feed ratio(G:F)and the digestibility of crude protein,amino acids and dietary fiber of finishing pigs compared with UFR.Additionally,FR supplementation significantly(P<0.05)increased total antioxidant capacity,the activities of superoxide dismutase and catalase,and decreased the content of malonaldehyde in serum.Furthermore,FR remarkably(P<0.05)increased serum levels of Ig G,antiinflammatory cytokines(IL-22 and IL-23)and reduced pro-inflammatory cytokines(TNF-α,IL-1β and INF-γ).The decrease of serum diamine oxidase activity and serum D-lactate content in the FR group(P<0.05)suggested an improvement in intestinal permeability.Supplementation of FR also elevated the content of acetate and butyrate in feces(P<0.05).Moreover,FR enhanced gut microbial richness and the abundance of fiber-degrading bacteria such as Clostridium butyricum and Lactobacillus amylovorus.Correlation analyses indicated dietary fiber in FR was associated with improvements in immune status,intestinal permeability and the level of butyrate-producing microbe C.butyricum,which was also verified by the in vitro fermentation analysis.These findings provided an experimental and theoretical basis for the application of fermented DFRB in finishing pigs.
基金financially supported by Joint Program of Beijing Natural Science Foundation Committee and Beijing Education Committee(KZ201810011017)Beijing Excellent Talent Training Project(2017000020124G100).
文摘Defatted chickpea flour(DCF),which is a by-product of chickpea oil extraction industry,is rich in nutrients that are beneficial to human health.In this study,the effects of temperature and DCF variation on the rheological properties,water holding capacity,freeze-thaw stability and microstructure of DCF heat induced gels were investigated.The results showed that the viscoelasticity,frequency dependence,and resistance strength of heat induced gels increased significantly with the increase of temperature and DCF variation.The degree of denaturation and water retention of heat induced gels increased significantly with increased variables within the temperature and variation windows of 75℃ to 95℃ and 13%to 21%.The CLSM results revealed that variations of both temperature and DCF variation could cause the proteins in the heat induced gels to aggregate gradually and to form protein aggregations.When temperature or variation exceeded certain value(85℃ or 17%),the protein aggregations broke up and the protein clusters became smaller and more homogeneous.Therefore,the heat induced gels presented better water holding capacity,viscoelasticity,structural stability and gel property at a temperature of 95℃ or a DCF variation of 21%within the present experimental range.
基金partially granted by the Hi-Ga project,which is part of the programme of the Green ERA-Hub that has received funding from the European Union’s Horizon Europe research and innovation programme under Grant Agreement No.101056828self-financed by the DINAMIC team of the UMR Herbivores(INRAE,VetAgro Sup,Saint-Genès-Champanelle,France)。
文摘Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibility and fatty acid(FA)biohydrogenation is scant.This trial aims to investigate the effects(i)of drying temperature of fullfat Hermetia illucens(HI)and Tenebrio molitor(TM)meals,and(ii)of residual ether extract(EE)content of defatted HI and TM meals,on their fermentation characteristics and FA of rumen digesta after 24-h in vitro rumen incubation.Methods The tested full-fat meals included four HI and four TM meals obtained applying drying temperatures ranging from 30℃ to 70℃,while the tested defatted meals consisted of five HI and two TM meals containing a residual EE content ranging from 4.7 to 19.7 g EE/100 g dry matter(DM).The applied statistical models(GLM ANOVA)tested the effects of insect species,drying temperature(full-fat meals)or EE content(defatted meals),and their interaction.Results Drying temperature had minor effects on in vitro ruminal digestibility and FA profile of rumen digesta.Irrespective of insect species,increasing the drying temperature led to a reduction of in vitro degradation of proteins from insect meals,as outlined by the significant decrease in ammonia production(-0.009 mmol/g DM and-0.126 g/100 g total N for each additional 1℃).Irrespective of insect species,defatting increased total gas,volatile fatty acids(VFA)and CH_(4) productions,and the proportions of total saturated and branched-chain FA in rumen digesta(+0.038 mmol/g DM,+0.063 mmol/g DM,+12.9μmol/g DM,+0.18 g/100 g FA,and+0.19 g/100 g FA for each reduced 1 g EE/100 g DM,respectively),and reduced the proportion of total PUFA(-0.12 g/100 g FA).Conclusions The applied drying temperatures of full-fat insect meals are too low to exert impactful effects on rumen digestibility and FA biohydrogenation.Fat lowered fermentation activity,probably because of an inhibitory effect on rumen microbiota.The increased ruminal digestibility of defatted insect meals suggests that they can be more suitable to be used in ruminant nutrition than full-fat ones.
