Oaks(Quercus spp.)provide an important food source for many wildlife species throughout the fall and winter.Most research evaluating oak masting patterns and the subsequent behavioral responses of wildlife focuses on ...Oaks(Quercus spp.)provide an important food source for many wildlife species throughout the fall and winter.Most research evaluating oak masting patterns and the subsequent behavioral responses of wildlife focuses on the annual temporal scale.However,patterns in masting at the seasonal temporal scale may be important for wildlife behavior.We designed a study quantifying seasonal oak masting patterns of 3 oak species(water oak,Q.nigra;laurel oak,Q.laurifolia;and swamp chestnut oak,Q.michauxii)and linking those patterns to visitation and feeding behavior of 3 primary consumers(white-tailed deer,Odocoileus virginianus;gray squirrel,Sciurus carolinensis;and raccoon,Procyon lotor).We used seed traps to monitor the seasonal masting pattern of 205 trees in the fall of 2021 and 2022 and used camera traps concurrently to monitor wildlife behavior associated with a subset of 30 trees.Seasonal masting patterns differed between oak species both within a season and across years,and the timing of mast varied within oak species across years.White-tailed deer tended to visit swamp chestnut oak as the number of acorns increased and consumed their acorns.Gray squirrels and raccoons tended to visit laurel oak and consume water oak acorns with gray squirrels being more likely to consume as the number of acorns increased.Our results indicate that evaluating acorn production at multiple temporal scales may be necessary to fully understand oak masting relationships with wildlife.Furthermore,differences in wildlife behavior based on oak species may have important implications for oak regeneration.展开更多
Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endang...Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endangered species,and their survival is also greatly challenged by various high incidence and high mortality respiratory and intestinal diseases such as septic pneumonia and enteritis.Accumulating evidence has demonstrated that Akkermannia muciniphila(AKK)is a promising probiotic,and we wondered whether AKK could be used as a food additive in animal breeding pro-grammes to help prevent intestinal diseases.Methods:We isolated one AKK strain from musk deer feces(AKK-D)using an im-proved enrichment medium combined with real-time PCR.After confirmation by 16S rRNA gene sequencing,a series of in vitro tests was conducted to evaluate the probiotic effects of AKK-D by assessing its reproductive capability,simulated gas-trointestinal fluid tolerance,acid and bile salt resistance,self-aggregation ability,hy-drophobicity,antibiotic sensitivity,hemolysis,harmful metabolite production,biofilm formation ability,and bacterial adhesion to gastrointestinal mucosa.Results:The AKK-D strain has a probiotic function similar to that of the standard strain in humans(AKK-H).An in vivo study found that AKK-D significantly amelio-rated symptoms in the enterotoxigenic Escherichia coli(ETEC)-induced murine diar-rhea model.AKK-D improved organ damage,inhibited inflammatory responses,and improved intestinal barrier permeability.Additionally,AKK-D promoted the reconsti-tution and maintenance of the homeostasis of gut microflora,as indicated by the fact that AKK-D-treated mice showed a decrease in Bacteroidetes and an increase in the proportion of other beneficial bacteria like Muribaculaceae,Muribaculum,and unclas-sified f_Lachnospiaceae compared with the diarrhea model mice.Conclusion:Taken together,our data show that this novel AKK-D strain might be a potential probiotic for use in musk deer breeding,although further extensive system-atic research is still needed.展开更多
A milu deer crosses a river in Dafeng,Jiangsu Province,on 2 July.The city is home to the world’s largest milu deer nature reserve,covering 78,000 hectares and hosting the largest wild population of the species globally.
1 In a town of fewer than 1,000 people,it can be hard to keep a secret.And yet no one in McBride,a mountain community in British Columbia,can figure out how a local deer came to be wearing a reflective jacket or why t...1 In a town of fewer than 1,000 people,it can be hard to keep a secret.And yet no one in McBride,a mountain community in British Columbia,can figure out how a local deer came to be wearing a reflective jacket or why the deer has been so hard to track down.展开更多
City of Muskogee Animal Control Supervisor Phil Blair is known for his devotion to animals,but his quick thinking this week took that commitment to a whole new level-saving the life of an unborn baby deer after a trag...City of Muskogee Animal Control Supervisor Phil Blair is known for his devotion to animals,but his quick thinking this week took that commitment to a whole new level-saving the life of an unborn baby deer after a tragic accident.Blair was called to the scene of a deer struck by a car.Upon arrival,he found the mother deer had suffered four broken legs and sadly had to be put down to prevent further suffering.展开更多
The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational re...The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational restraints on the proteins or their complexes.The rigid connection of the nitroxide spin label to the protein improves the accuracy and precision of distance measurement.We report a new spin labelling approach by formation of thioester bond between nitroxide(NO)spin label,NOAI(NO spin labels activated by acetylimidazole),and a protein thiol,and this spin labeling method has demonstrated high performance in DEER distance measurement on proteins.The results showed that NOAI has shorter connection to the protein ligation site than 2,2,5,5-tetramethyl-pyrroline-1-oxyl methanethiosulfonate(MTSL)and 3-maleimido-proxyl(M-Prox)in the respective protein conjugate and produces narrower distance distributions for the tested proteins including ubiquitin(Ub),immunoglobulin-binding b1 domain of streptococcal protein G(GB1),and second mitochondria-derived activator of caspases(Smac).The NOAI protein conjugate connected by a thioester bond is resistant to reducing reagent and offers highfidelity DEER distance measurements in cell lysates.展开更多
Named entity recognition(NER)in musk deer domain is the extraction of specific types of entities from unstructured texts,constituting a fundamental component of the knowledge graph,Q&A system,and text summarizatio...Named entity recognition(NER)in musk deer domain is the extraction of specific types of entities from unstructured texts,constituting a fundamental component of the knowledge graph,Q&A system,and text summarization system of musk deer domain.Due to limited annotated data,diverse entity types,and the ambiguity of Chinese word boundaries in musk deer domain NER,we present a novel NER model,CAELF-GP,which is based on cross-attention mechanism enhanced lexical features(CAELF).Specifically,we employ BERT as a character encoder and advocate the integration of external lexical information at the character representation layer.In the feature fusion module,instead of indiscriminately merging external dictionary information,we innovatively adopted a feature fusion method based on a cross-attention mechanism,which guides the model to focus on important lexical information by calculating the correlation between each character and its corresponding word sets.This module enhances the model’s semantic representation ability and entity boundary recognition capability.Ultimately,we introduce the decoding module of GlobalPointer(GP)for entity type recognition,capable of identifying both nested and non-nested entities.Since there is currently no publicly available dataset for the musk deer domain,we built a named entity recognition dataset for this domain by collecting relevant literature and working under the guidance of domain experts.The dataset facilitates the training and validation of the model and provides data foundation for subsequent related research.The model undergoes experimentation on two public datasets and the dataset of musk deer domain.The results show that it is superior to the baseline models,offering a promising technical avenue for the intelligent recognition of named entities in the musk deer domain.展开更多
This article takes the theory of power space as an starting point for an in-depth comparison of the clan hall in White Deer Plains and the scaffold in The Scarlet Letter.In White Deer Plains,The clan hall,as the core ...This article takes the theory of power space as an starting point for an in-depth comparison of the clan hall in White Deer Plains and the scaffold in The Scarlet Letter.In White Deer Plains,The clan hall,as the core place of family activities,has a spatial layout that implies a hierarchy of power,and the taboos it represents relate to various aspects such as marriage and bloodline,and maintains the order of the clan through a variety of disciplinary mechanisms.The scaffold in The Scarlet Letter is located in the town’s central square and is a symbol of Puritan social power and moral discipline,behind which Puritan taboos influence people’s behaviors and public punishments are used to achieve the discipline of the people.The research reveals the similarities and differences between the two in terms of sources of power,modes of operation and influence,and opens up new horizons for cross-cultural literary studies.展开更多
Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b ge...Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.展开更多
[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 p...[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.展开更多
Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine ...Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.展开更多
A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al（OH）3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The i...A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al（OH）3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk deer.The serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination test.The serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third vaccination.Young forest musk deer showed serum antibody titers which were obtained due to nursing.Young forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody titers.The serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days old.The maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic Escherichia coli strains（serotypes O4,O26,and O139）for 137 days after birth（during the nursing period and the period when the forest musk deer were susceptible to diseases）.展开更多
Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,b...Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.展开更多
[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the charac...[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.展开更多
文摘Oaks(Quercus spp.)provide an important food source for many wildlife species throughout the fall and winter.Most research evaluating oak masting patterns and the subsequent behavioral responses of wildlife focuses on the annual temporal scale.However,patterns in masting at the seasonal temporal scale may be important for wildlife behavior.We designed a study quantifying seasonal oak masting patterns of 3 oak species(water oak,Q.nigra;laurel oak,Q.laurifolia;and swamp chestnut oak,Q.michauxii)and linking those patterns to visitation and feeding behavior of 3 primary consumers(white-tailed deer,Odocoileus virginianus;gray squirrel,Sciurus carolinensis;and raccoon,Procyon lotor).We used seed traps to monitor the seasonal masting pattern of 205 trees in the fall of 2021 and 2022 and used camera traps concurrently to monitor wildlife behavior associated with a subset of 30 trees.Seasonal masting patterns differed between oak species both within a season and across years,and the timing of mast varied within oak species across years.White-tailed deer tended to visit swamp chestnut oak as the number of acorns increased and consumed their acorns.Gray squirrels and raccoons tended to visit laurel oak and consume water oak acorns with gray squirrels being more likely to consume as the number of acorns increased.Our results indicate that evaluating acorn production at multiple temporal scales may be necessary to fully understand oak masting relationships with wildlife.Furthermore,differences in wildlife behavior based on oak species may have important implications for oak regeneration.
基金This research was funded by Shaanxi Administration of Traditional Chinese Medicine Projects(2021-QYZL-01,2021-QYPT-001)Key R&D Project in Shaanxi Province(2023-YBSF-463)+1 种基金the Foundation of Science and Technology in Shaanxi Province(2020TD-050)the Fundamental Research Funds for the Central Universities(GK202205010).
文摘Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endangered species,and their survival is also greatly challenged by various high incidence and high mortality respiratory and intestinal diseases such as septic pneumonia and enteritis.Accumulating evidence has demonstrated that Akkermannia muciniphila(AKK)is a promising probiotic,and we wondered whether AKK could be used as a food additive in animal breeding pro-grammes to help prevent intestinal diseases.Methods:We isolated one AKK strain from musk deer feces(AKK-D)using an im-proved enrichment medium combined with real-time PCR.After confirmation by 16S rRNA gene sequencing,a series of in vitro tests was conducted to evaluate the probiotic effects of AKK-D by assessing its reproductive capability,simulated gas-trointestinal fluid tolerance,acid and bile salt resistance,self-aggregation ability,hy-drophobicity,antibiotic sensitivity,hemolysis,harmful metabolite production,biofilm formation ability,and bacterial adhesion to gastrointestinal mucosa.Results:The AKK-D strain has a probiotic function similar to that of the standard strain in humans(AKK-H).An in vivo study found that AKK-D significantly amelio-rated symptoms in the enterotoxigenic Escherichia coli(ETEC)-induced murine diar-rhea model.AKK-D improved organ damage,inhibited inflammatory responses,and improved intestinal barrier permeability.Additionally,AKK-D promoted the reconsti-tution and maintenance of the homeostasis of gut microflora,as indicated by the fact that AKK-D-treated mice showed a decrease in Bacteroidetes and an increase in the proportion of other beneficial bacteria like Muribaculaceae,Muribaculum,and unclas-sified f_Lachnospiaceae compared with the diarrhea model mice.Conclusion:Taken together,our data show that this novel AKK-D strain might be a potential probiotic for use in musk deer breeding,although further extensive system-atic research is still needed.
文摘A milu deer crosses a river in Dafeng,Jiangsu Province,on 2 July.The city is home to the world’s largest milu deer nature reserve,covering 78,000 hectares and hosting the largest wild population of the species globally.
文摘1 In a town of fewer than 1,000 people,it can be hard to keep a secret.And yet no one in McBride,a mountain community in British Columbia,can figure out how a local deer came to be wearing a reflective jacket or why the deer has been so hard to track down.
文摘City of Muskogee Animal Control Supervisor Phil Blair is known for his devotion to animals,but his quick thinking this week took that commitment to a whole new level-saving the life of an unborn baby deer after a tragic accident.Blair was called to the scene of a deer struck by a car.Upon arrival,he found the mother deer had suffered four broken legs and sadly had to be put down to prevent further suffering.
基金supported by National Natural Science Foundation of China(22161142018,21991081,22177056,and 22174074)the Ministry of Science and Technology of China(2021YFA1600304).
文摘The distance distributions between two site-specifically anchored spin labels in a protein,measured by pulsed electron-electron double resonance(PELDOR or DEER),provide rich sources of structural and conformational restraints on the proteins or their complexes.The rigid connection of the nitroxide spin label to the protein improves the accuracy and precision of distance measurement.We report a new spin labelling approach by formation of thioester bond between nitroxide(NO)spin label,NOAI(NO spin labels activated by acetylimidazole),and a protein thiol,and this spin labeling method has demonstrated high performance in DEER distance measurement on proteins.The results showed that NOAI has shorter connection to the protein ligation site than 2,2,5,5-tetramethyl-pyrroline-1-oxyl methanethiosulfonate(MTSL)and 3-maleimido-proxyl(M-Prox)in the respective protein conjugate and produces narrower distance distributions for the tested proteins including ubiquitin(Ub),immunoglobulin-binding b1 domain of streptococcal protein G(GB1),and second mitochondria-derived activator of caspases(Smac).The NOAI protein conjugate connected by a thioester bond is resistant to reducing reagent and offers highfidelity DEER distance measurements in cell lysates.
基金funded by 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University(No.BLRC2023C02).
文摘Named entity recognition(NER)in musk deer domain is the extraction of specific types of entities from unstructured texts,constituting a fundamental component of the knowledge graph,Q&A system,and text summarization system of musk deer domain.Due to limited annotated data,diverse entity types,and the ambiguity of Chinese word boundaries in musk deer domain NER,we present a novel NER model,CAELF-GP,which is based on cross-attention mechanism enhanced lexical features(CAELF).Specifically,we employ BERT as a character encoder and advocate the integration of external lexical information at the character representation layer.In the feature fusion module,instead of indiscriminately merging external dictionary information,we innovatively adopted a feature fusion method based on a cross-attention mechanism,which guides the model to focus on important lexical information by calculating the correlation between each character and its corresponding word sets.This module enhances the model’s semantic representation ability and entity boundary recognition capability.Ultimately,we introduce the decoding module of GlobalPointer(GP)for entity type recognition,capable of identifying both nested and non-nested entities.Since there is currently no publicly available dataset for the musk deer domain,we built a named entity recognition dataset for this domain by collecting relevant literature and working under the guidance of domain experts.The dataset facilitates the training and validation of the model and provides data foundation for subsequent related research.The model undergoes experimentation on two public datasets and the dataset of musk deer domain.The results show that it is superior to the baseline models,offering a promising technical avenue for the intelligent recognition of named entities in the musk deer domain.
文摘This article takes the theory of power space as an starting point for an in-depth comparison of the clan hall in White Deer Plains and the scaffold in The Scarlet Letter.In White Deer Plains,The clan hall,as the core place of family activities,has a spatial layout that implies a hierarchy of power,and the taboos it represents relate to various aspects such as marriage and bloodline,and maintains the order of the clan through a variety of disciplinary mechanisms.The scaffold in The Scarlet Letter is located in the town’s central square and is a symbol of Puritan social power and moral discipline,behind which Puritan taboos influence people’s behaviors and public punishments are used to achieve the discipline of the people.The research reveals the similarities and differences between the two in terms of sources of power,modes of operation and influence,and opens up new horizons for cross-cultural literary studies.
基金This work received supportsfrom Natural Science Foundation of Yun nan Science Technology Committee granted to SuBingpartlys
文摘Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.
基金Supported by Youth Foundation of Education Department in Sichuan Province (07ZB060)Youth Science and Technology Innovation Fund in Sichuan Agricultural University~~
文摘[Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. [Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. [Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. [Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding.
文摘Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.
基金Supported by Youth Foundation of Education Department in Sichuan Province(07ZB060)Scientific and Technological Supporting Project in Science and Technology Bureau of Sichuan Province(2009SZ0228)~~
文摘A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al（OH）3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk deer.The serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination test.The serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third vaccination.Young forest musk deer showed serum antibody titers which were obtained due to nursing.Young forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody titers.The serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days old.The maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic Escherichia coli strains（serotypes O4,O26,and O139）for 137 days after birth（during the nursing period and the period when the forest musk deer were susceptible to diseases）.
文摘Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.
基金Supported by the National Natural Science Foundation of China(31060152)the Natural Science Foundation of the Xinjiang Uygur Autonomous Region,China(2010211A02)the Key Program for Animal Sciences of Xinjiang University,China~~
文摘[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.
