Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer sc...Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer scale.However,the divergence of the excited Gaussian beam limits the depth-of-field of the system to less than 100μm,which hinders accurate three-dimensional imaging of living tissues and restrictsits applicability in biological research.Therefore,there is an urgent need for an effective method to enhance the depth-of-field without altering the hardware configuration.This paper presents a photoacoustic microscopy depth-of-field extension method and system based on three-dimensional continuity and sparsity deconvolution.This method utilizes a depth-varying point spread function and incorporates continuity and sparsity con-straints into the deconvolution process to mitigate the effect of background noise,enhancing the stability and accuracy of the depth-of-field extension.Experimental results using tungsten wire phantoms suggest that the depth-of-field of system can be extended to 650 pm,which is 7.2 times greater than conventional system,while improving the resolution of the defocused region by an average factor of 3.5.Furthermore,experiments on zebrafish and nude mouse ears with irregular topologies demonstrate that the proposed method successfully overcomes image blurring and the loss of structural information due to limited depth-of-field.All the results suggest that the system with higher lateral resolution and enhanced depth-of-field has significant potential for a wide range of practical biomedical applications.展开更多
Traditional deconvolution methods based on single-channel inversion do not consider the spatial structural relation between channels,and hence,they yield high-resolution results with the existing transverse inconsiste...Traditional deconvolution methods based on single-channel inversion do not consider the spatial structural relation between channels,and hence,they yield high-resolution results with the existing transverse inconsistency or discontinuity.Therefore,in this study,the local dip angle was used to obtain the structural information and construct the spatial structurally constraint operator.This operator is then introduced into multichannel deconvolution as a regularization operator to improve the resolution and maintain the transverse continuity of seismic data.Model tests and actual seismic data processing have demonstrated the effectiveness and practicability of this method.展开更多
文摘针对传统诊断模型对含有周期性故障脉冲的振动信号诊断率较低、振动信号空间特征和时序特征得不到有效提取等问题,文中提出了一种基于最大相关峭度解卷积(maximum correlation kurtosis deconvolution,MCKD)和改进的双通道卷积神经网络-长短期记忆神经网络(convolutional neural network-long short term memory network,CNN-LSTM)相结合的轴承故障诊断模型。使用MCKD去除噪音干扰、增强信号周期性脉冲、重构原始信号,将重构后的信号分别同时输入到CNN和LSTM通道中,利用CNN强大的空间特征提取能力和LSTM挖掘数据时序特征能力,将二者得到的特征信息进行融合,最后输入到Softmax完成故障分类。在凯斯西储大学轴承数据集上进行验证,实验结果表明,该方法诊断性能优于其他主流模型,能够有效提高轴承诊断精度。
基金supported by the National Key R&D Program of China[Grant No.2022YFC2402400]the National Natural Science Foundation of China[Grant No.62275062]+2 种基金Project of Shandong Innovation and Startup Community of High-end Medical Apparatus and Instruments[Grant Nos.2023-SGTTXM-002 and 2024-SGTTXM-005]the Shandong Province Technology Innovation Guidance Plan(Central Leading Local Science and Technology Development Fund)[Grant No.YDZX2023115]the Taishan Scholar Special Funding Project of Shandong Province,and the Shandong Laboratory of Advanced Biomaterials and Medical Devices in Weihai[Grant No.ZL202402].
文摘Optical-resolution photoacoustic microscopy is a novel imaging technique that combines the advantages of optical and ultrasound imaging,enabling high-resolution visualization of biological tissues at the micrometer scale.However,the divergence of the excited Gaussian beam limits the depth-of-field of the system to less than 100μm,which hinders accurate three-dimensional imaging of living tissues and restrictsits applicability in biological research.Therefore,there is an urgent need for an effective method to enhance the depth-of-field without altering the hardware configuration.This paper presents a photoacoustic microscopy depth-of-field extension method and system based on three-dimensional continuity and sparsity deconvolution.This method utilizes a depth-varying point spread function and incorporates continuity and sparsity con-straints into the deconvolution process to mitigate the effect of background noise,enhancing the stability and accuracy of the depth-of-field extension.Experimental results using tungsten wire phantoms suggest that the depth-of-field of system can be extended to 650 pm,which is 7.2 times greater than conventional system,while improving the resolution of the defocused region by an average factor of 3.5.Furthermore,experiments on zebrafish and nude mouse ears with irregular topologies demonstrate that the proposed method successfully overcomes image blurring and the loss of structural information due to limited depth-of-field.All the results suggest that the system with higher lateral resolution and enhanced depth-of-field has significant potential for a wide range of practical biomedical applications.
基金supported by the basic and forward-looking project(No.2023YQX302)。
文摘Traditional deconvolution methods based on single-channel inversion do not consider the spatial structural relation between channels,and hence,they yield high-resolution results with the existing transverse inconsistency or discontinuity.Therefore,in this study,the local dip angle was used to obtain the structural information and construct the spatial structurally constraint operator.This operator is then introduced into multichannel deconvolution as a regularization operator to improve the resolution and maintain the transverse continuity of seismic data.Model tests and actual seismic data processing have demonstrated the effectiveness and practicability of this method.
