Biosurfactants are amphiphilic components applied in various fields,one of which is preserving agricultural products.Biosurfactants can be used as additives in edible coatings to prevent microbial adhesion by reducing...Biosurfactants are amphiphilic components applied in various fields,one of which is preserving agricultural products.Biosurfactants can be used as additives in edible coatings to prevent microbial adhesion by reducing surface tension on the fruit surface.They also play a role in increasing the moisture barrier and slowing down the physiological changes of fruit.Nonetheless,biosurfactants were often produced by non-GRAS bacteria.GRAS biosurfactant-producing bacteria are urgently needed for application in food products.This study aims to isolate and characterize biosurfactant-producing strains of lactic acid bacteria from Dadiah and evaluate their efficacy in reducing microbial adhesion on strawberries.From four selected isolates found in Dadiah,Lactiplantibacillus plantarum was the best candidate,producing biosurfactant with index emulsification(EI24)of 64.48±0.37% with a CMC of 1 g/l.LC-MS and FT-IR characterized the biosurfactants as glycolipids.The rate of antibacterial and anti-adhesive tests on Escherichia coli ATCC 8739 were 18.79±5.23% and 55.26±0.02%,while on Staphylococcus aureus ATCC 6738 were 31.60±2.63% and 74.63±0.00% at a concentration of 3000 mg/l.However,no anti-fungal activity against Rhizopus stolonifer and Aspergillus niger was observed at the same concentration.Compared to SDS,biosurfactants demonstrated non-toxicity with an LC50 value of 2.94 x 10^(8) mg/l.A combination of 1%chitosan reduced the number of fungal cells by 29.76±3.25% on 125 mg/l biosurfactant and bacterial cells by 31.91±5.06% on 1000 mg/l biosurfactant after 10 days,indicating their potential to reduce fruit spoilage and enhance microbial anti-adhesive properties of edible coatings.展开更多
基金supported by Riset Unggulan ITB 2024 Award Number 959/IT1.B07.1/TA.00/2024 from Institut Teknologi Bandung,Indonesia。
文摘Biosurfactants are amphiphilic components applied in various fields,one of which is preserving agricultural products.Biosurfactants can be used as additives in edible coatings to prevent microbial adhesion by reducing surface tension on the fruit surface.They also play a role in increasing the moisture barrier and slowing down the physiological changes of fruit.Nonetheless,biosurfactants were often produced by non-GRAS bacteria.GRAS biosurfactant-producing bacteria are urgently needed for application in food products.This study aims to isolate and characterize biosurfactant-producing strains of lactic acid bacteria from Dadiah and evaluate their efficacy in reducing microbial adhesion on strawberries.From four selected isolates found in Dadiah,Lactiplantibacillus plantarum was the best candidate,producing biosurfactant with index emulsification(EI24)of 64.48±0.37% with a CMC of 1 g/l.LC-MS and FT-IR characterized the biosurfactants as glycolipids.The rate of antibacterial and anti-adhesive tests on Escherichia coli ATCC 8739 were 18.79±5.23% and 55.26±0.02%,while on Staphylococcus aureus ATCC 6738 were 31.60±2.63% and 74.63±0.00% at a concentration of 3000 mg/l.However,no anti-fungal activity against Rhizopus stolonifer and Aspergillus niger was observed at the same concentration.Compared to SDS,biosurfactants demonstrated non-toxicity with an LC50 value of 2.94 x 10^(8) mg/l.A combination of 1%chitosan reduced the number of fungal cells by 29.76±3.25% on 125 mg/l biosurfactant and bacterial cells by 31.91±5.06% on 1000 mg/l biosurfactant after 10 days,indicating their potential to reduce fruit spoilage and enhance microbial anti-adhesive properties of edible coatings.
