The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia ...The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia solani and on the mycelium growth, conidial formation, germination, appressorium formation of Magnaporthe grisea. The results showed that the antifungal monomer component DZP8 has strong antifungal effect on both the R. solani and M. grisea. The EC50 and EC90 of DZP8 were 1.81 and 3.35 μg/ml on Ft. solani respectively, and 37.01 and 136.21 μg/ml on M. grisea respectively. Under the treatment of 48.01 μg/ml DZP8, the sclerotium formation rate of R. solani was just 39.21%, the formation time delayed by 216 h and the dry weight decreased by 81.37% in comparison the con- trol; and 33.51 μg/ml DZP8 significantly inhibited the sclerotium germination. In the presence of 160.08 μg/ml DZP8, the sporulation of M. grisea was just 9.29% of control sample; 20.14 μg/ml DZP8 inhibited the conidial germination suppression rate by 95.16%, and the appressorium formation by 100%.展开更多
In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani, the effects of DZP8 on mycelial morphology were investigated under light microscop...In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani, the effects of DZP8 on mycelial morphology were investigated under light microscope, and the effects of DZP8 on mycelial inclusion leakage and cell membrane damage of myceli-um were determined. The results showed that DZP8 caused a series of changes in mycelial morphology of R. solani in liquid culture condition. DZP8 treatments with concentrations of 1.81 and 3.35 μg/mL for 24 h caused big vacuole, rough surface and more inclusions of mycelium. With the treatment time prolonging, the mycelium distorted and appeared irregular constriction. DZP8 treatment with concentration of 20.10 μg/mL led to the increase of conductivity of mycelium culture liquid, leakage of soluble sugar and protein, and the lipid peroxidation of mycelium membrane. It was found that DZP8 at a very low concentration could cause changes of mycelial morphology of R. solani, while only a certain concentration could cause significant damage to cell membrane. This indicated that cell membrane might be one of the action sites of DZP8, and it might have other action sites or mechanism.展开更多
The present work aimed to demonstrate that the dietary content of the drug diazepam, a common benzodiazepine, regulates many aspect of feed efficiency of the Nile tilapia Oreochromis niloticus a favorite in fish produ...The present work aimed to demonstrate that the dietary content of the drug diazepam, a common benzodiazepine, regulates many aspect of feed efficiency of the Nile tilapia Oreochromis niloticus a favorite in fish production, and also to test introducing a simple new model in the investigation of the biological mechanisms of drug addiction. Diazepam was added to the basic diet at different levels （1.5, 3.0, 5.0, 7.0, 8.0, 9.0, 10.0, 12.0, 14.0 and 16.0 mg/100 g diet）. The experiments lasted for twelve weeks. The results obtained suggested that most of the diazepam doses were able to stimulate the growth parameters of O. niloticus, especially at 12.0 mg/100 gdiet. At some selected doses, diazepam reduces AChE specific activities in the tilapia brain and the inhibition was higher at the 12.0 mg dose. The clearance of diazepam in fish muscles and skin with lapse of time indicated that the fish treatment poses no health risk to the consumer. The recommended dose is the 12 mg DZP/100 mg diet. Finally, tilapia can be used as a new powerful model for the study of fish growth, which could provide insights into the mechanisms of drug addiction.展开更多
基金Supported by National Natural Science Foundation of China(31071724)Natural Science Foundation of Jiangxi Province(2010GZN0037)~~
文摘The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia solani and on the mycelium growth, conidial formation, germination, appressorium formation of Magnaporthe grisea. The results showed that the antifungal monomer component DZP8 has strong antifungal effect on both the R. solani and M. grisea. The EC50 and EC90 of DZP8 were 1.81 and 3.35 μg/ml on Ft. solani respectively, and 37.01 and 136.21 μg/ml on M. grisea respectively. Under the treatment of 48.01 μg/ml DZP8, the sclerotium formation rate of R. solani was just 39.21%, the formation time delayed by 216 h and the dry weight decreased by 81.37% in comparison the con- trol; and 33.51 μg/ml DZP8 significantly inhibited the sclerotium germination. In the presence of 160.08 μg/ml DZP8, the sporulation of M. grisea was just 9.29% of control sample; 20.14 μg/ml DZP8 inhibited the conidial germination suppression rate by 95.16%, and the appressorium formation by 100%.
基金Supported by General Program of National Natural Science Foundation of China(31071724)Natural Science Foundation of Jiangxi Province(2010GZN0037)
文摘In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani, the effects of DZP8 on mycelial morphology were investigated under light microscope, and the effects of DZP8 on mycelial inclusion leakage and cell membrane damage of myceli-um were determined. The results showed that DZP8 caused a series of changes in mycelial morphology of R. solani in liquid culture condition. DZP8 treatments with concentrations of 1.81 and 3.35 μg/mL for 24 h caused big vacuole, rough surface and more inclusions of mycelium. With the treatment time prolonging, the mycelium distorted and appeared irregular constriction. DZP8 treatment with concentration of 20.10 μg/mL led to the increase of conductivity of mycelium culture liquid, leakage of soluble sugar and protein, and the lipid peroxidation of mycelium membrane. It was found that DZP8 at a very low concentration could cause changes of mycelial morphology of R. solani, while only a certain concentration could cause significant damage to cell membrane. This indicated that cell membrane might be one of the action sites of DZP8, and it might have other action sites or mechanism.
文摘The present work aimed to demonstrate that the dietary content of the drug diazepam, a common benzodiazepine, regulates many aspect of feed efficiency of the Nile tilapia Oreochromis niloticus a favorite in fish production, and also to test introducing a simple new model in the investigation of the biological mechanisms of drug addiction. Diazepam was added to the basic diet at different levels （1.5, 3.0, 5.0, 7.0, 8.0, 9.0, 10.0, 12.0, 14.0 and 16.0 mg/100 g diet）. The experiments lasted for twelve weeks. The results obtained suggested that most of the diazepam doses were able to stimulate the growth parameters of O. niloticus, especially at 12.0 mg/100 gdiet. At some selected doses, diazepam reduces AChE specific activities in the tilapia brain and the inhibition was higher at the 12.0 mg dose. The clearance of diazepam in fish muscles and skin with lapse of time indicated that the fish treatment poses no health risk to the consumer. The recommended dose is the 12 mg DZP/100 mg diet. Finally, tilapia can be used as a new powerful model for the study of fish growth, which could provide insights into the mechanisms of drug addiction.
