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辅助性T细胞和调节性T细胞在天疱疮发病中的作用 被引量:4
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作者 许人超 朱海琴 +1 位作者 潘萌 郑捷 《临床皮肤科杂志》 CAS CSCD 北大核心 2013年第12期716-719,共4页
目的:探讨辅助性T细胞17(Th17)和调节性T(Treg)细胞在天疱疮发病中的免疫介导机制。方法:采用FACS检测51例天疱疮患者外周血中CD3^+CD8^-T细胞(即CD3^+CD4^+T细胞)IL-17A表达,以及CD4^+CD25hiFoxp3^+Treg细胞水平,并与26例正常对照比较... 目的:探讨辅助性T细胞17(Th17)和调节性T(Treg)细胞在天疱疮发病中的免疫介导机制。方法:采用FACS检测51例天疱疮患者外周血中CD3^+CD8^-T细胞(即CD3^+CD4^+T细胞)IL-17A表达,以及CD4^+CD25hiFoxp3^+Treg细胞水平,并与26例正常对照比较;采用ELISA法检测患者血清中特异性抗桥粒芯糖蛋白(Dsg)1、Dsg3抗体滴度、亚型,分析其与Th17和Treg细胞相关性。结果:天疱疮患者外周血Th17细胞比例显著高于正常对照组(P=0.014),并且急性期升高更为明显,急性期显著高于正常对照组(P=0.015);患者外周血Treg细胞比例显著低于正常对照组(P<0.001),并且急性期和稳定期患者均显著低于正常对照(P值分别为<0.001和=0.015),同时急性期患者显著低于稳定期患者(P=0.024);天疱疮患者外周血Th17与Treg细胞比例呈负相关(r=-0.532,P<0.001)。急性期患者抗Dsg1和Dsg3抗体滴度水平显著高于稳定期,并且急性期患者抗体亚型IgG4/IgG1比值显著升高。Dsg1和Dsg3抗体滴度水平及抗体亚型与Th17和Treg细胞比例无相关性。结论:天疱疮患者外周血Th17与Treg细胞失平衡,参与天疱疮致病。急性期患者抗体滴度显著升高,其中IgG4亚型是主要的致病性抗体。 展开更多
关键词 天疱疮 TH17细胞 调节性T细胞 抗Dsg1抗体 抗Dsg3抗体
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Improvement of Glacial Lakes Detection under Shadow Environment Using ASTER Data in Himalayas, Nepal 被引量:2
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作者 CHEN Wenbo FUKUI Hiromichi +1 位作者 DOKO Tomoko GU Xingfa 《Chinese Geographical Science》 SCIE CSCD 2013年第2期216-226,共11页
The detection of glacial lake change in the Himalayas, Nepal is extremely significant since the glacial lake change is one of the crucial indicators of global climate change in this area, where is the most sensitive a... The detection of glacial lake change in the Himalayas, Nepal is extremely significant since the glacial lake change is one of the crucial indicators of global climate change in this area, where is the most sensitive area of the global climate changes. In the Hima- layas, some of glacial lakes are covered by the dark mountains' shadow because of their location. Therefore, these lakes can not be de- tected by conventional method such as Normalized Difference Water Index (NDWI), because the reflectance feature of shadowed glacial lake is different comparing to the ones which are located in the open flat area. The shadow causes two major problems: 1) glacial lakes which are covered by shadow completely result in underestimation of the number of glacial lakes; 2) glacial lakes which are partly iden- tified are considered to undervalue the area of glacial lakes. The aim of this study is to develop a new model, named Detection of Shadowed Glacial Lakes (DSGL) model, to identify glacial lakes under the shadow environment by using Advanced Space-borne Ther- mal Emission and Reflection Radiometer (ASTER) data in the Himalayas, Nepal. The DSGL model is based on integration of two dif- ferent modifications of NDWI, namely NDWls model and NDWIshe model. NDWI~ is defined as integration of the NDWI and slope analysis and used for detecting non-shadowed lake in the mountain area. The NDWIshe is proposed as a new methodology to overcome the weakness of NDWI~ on identifying shadowed lakes in highly elevated mountainous area such as the Himalayas. The first step of the NDWIshe is to enhance the data from ASTER 1B using the histogram equalization (HE) method, and its outcome product is named AS- TERho. We used the ASTERhe for calculating the NDWIhc and the NDWIshe. Integrated with terrain analysis using Digital Elevation Model (DEM) data, the NDWIshe can be used to identify the shadowed glacial lakes in the Himalayas. NDWIs value of 0.41 is used to identify the glacier lake (NDWI~ 〉 0.41), and 0.3 of NDWIshe is used to identify the shadowed glacier lake (NDWIsho 〈 0.3). The DSGL model was proved to be able to classify the glacial lakes more accurately, while the NDWI model had tendency to underestimate the presence of actual glacial lakes. Correct classification rate regarding the products from NDWI model and DSGL model were 57% and 99%, respectively. The results of this paper demonstrated that the DSGL model is promising to detect glacial lakes in the shadowed en- vironment at high mountains. 展开更多
关键词 glacial lake shadow environment dsgl model ASTER remote sensing HIMALAYAS Nepal
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酶联免疫吸附法和间接免疫荧光法检测天疱疮抗体的比较 被引量:2
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作者 李婷婷 赵娟 康晓静 《新疆医学》 2017年第11期1230-1232,共3页
目的比较酶联免疫吸附法(ELISA)和间接免疫荧光法(IIF)检测天疱疮抗体的临床应用价值。方法选择79例天疱疮患者(试验组)和同期排除天疱疮的79例对照人群(对照组),采用间接免疫荧光法(IIF)检测抗表皮棘细胞桥粒抗体,采用酶联免疫吸附法(E... 目的比较酶联免疫吸附法(ELISA)和间接免疫荧光法(IIF)检测天疱疮抗体的临床应用价值。方法选择79例天疱疮患者(试验组)和同期排除天疱疮的79例对照人群(对照组),采用间接免疫荧光法(IIF)检测抗表皮棘细胞桥粒抗体,采用酶联免疫吸附法(ELISA)检测抗Dsg1和Dsg3抗体,比较两种检测指标的敏感性、特异性、阳性预测值(PPV)、阴性预测值(NPV)和诊断符合率。结果试验组和对照组抗Dsg1抗体阳性率分别为75.9%(60/79)和2.5%(2/79),抗Dsg3抗体阳性率分别为67.1%(53/79)和3.8%(3/79),抗表皮棘细胞桥粒抗体阳性率分别为82.3%(65/79)和2.5%(2/79)。在天疱疮患者中抗Dsg1抗体的敏感度、特异度、PPV、NPV、诊断符合率分别为75.9%、97.5%、96.8%、80.2%、86.7%。抗Dsg3抗体分别为67.1%、96.2%、94.6%、74.5%、79.7%,抗表皮棘细胞桥粒抗体分别为82.3%、97.5%、97.0%、84.6%、89.9%。3种指标差异无统计学意义(P>0.05)。结论 ELISA法检测抗Dsg1、Dsg3抗体与IIF法检测抗表皮棘细胞桥粒抗体在天疱疮的诊断中有较好的一致性,能相互补充,提高诊断阳性率。 展开更多
关键词 天疱疮 抗表皮棘细胞桥粒抗体 抗Dsg1抗体 抗Dsg3抗体
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