Low reactivity and appropriate wettability between molten superalloys and ceramic materials are crucial for the production of high-quality superalloy castings.The sessile-drop experiment was employed to systematically...Low reactivity and appropriate wettability between molten superalloys and ceramic materials are crucial for the production of high-quality superalloy castings.The sessile-drop experiment was employed to systematically investigate the interfacial reaction and wettability between the 4777DS1 superalloy and SiO_(2)-based ceramic core at various temperatures(1,480℃,1,500℃,1,520℃,and 1,550℃).The wetting behavior and interfacial reaction products at different temperatures were analyzed by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),and X-ray diffraction(XRD).The interfacial reaction process and products were discussed,and the thermodynamic behavior and interfacial reaction mechanisms were elucidated.The results demonstrate that the wetting behavior and interfacial reaction between the 4777DS1 alloy and the ceramic core are significantly influenced by temperature.The wettability angle exhibits a trend of initial decrease followed by an increase with rising temperature,reaching a maximum of 139°at 1,480℃,indicating poorer wettability of the 4777DS1 superalloy with the ceramic core and better casting properties at this specific temperature.The most intense interfacial reaction occurs at 1,520℃,resulting in the formation of the main interfacial reaction products such as Al_(2)O_(3),SiO_(2),and HfO_(2).Additionally,some crystal-like products rich in Si and Hf distribute on the reaction layer.展开更多
目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3...目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3DL1的mRNA含量。结果通过电泳和实时定量PCR的熔解曲线明确了KIR3DS1和KIR3DL1引物,其特异性好,无杂带。应用TA克隆建立的KIR定量标准品线性关系好,可检测待测物的范围大。应用临床标本可检测出两组标本有显著差异,符合临床预期。结论本文建立的KIR mRNA定量检测方法,可以定量检测活化和抑制性KIR,可以进一步研究不同疾病进展的机制以及预测疾病的临床转归。展开更多
基金supported by the fund of State Key Laboratory of Clean and Efficient Turbomachinery Power Equipment(No.DEC8300CG202210353EE280297)the China Postdoctoral Science Foundation(No.2021M692555)+1 种基金the Shaanxi Province Qinchuangyuan‘Scientists+Engineers’Team Building Project(No.2023KXJ-266)the Fundamental Research Funds for the Central Universities(No.xzy012023145)。
文摘Low reactivity and appropriate wettability between molten superalloys and ceramic materials are crucial for the production of high-quality superalloy castings.The sessile-drop experiment was employed to systematically investigate the interfacial reaction and wettability between the 4777DS1 superalloy and SiO_(2)-based ceramic core at various temperatures(1,480℃,1,500℃,1,520℃,and 1,550℃).The wetting behavior and interfacial reaction products at different temperatures were analyzed by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),and X-ray diffraction(XRD).The interfacial reaction process and products were discussed,and the thermodynamic behavior and interfacial reaction mechanisms were elucidated.The results demonstrate that the wetting behavior and interfacial reaction between the 4777DS1 alloy and the ceramic core are significantly influenced by temperature.The wettability angle exhibits a trend of initial decrease followed by an increase with rising temperature,reaching a maximum of 139°at 1,480℃,indicating poorer wettability of the 4777DS1 superalloy with the ceramic core and better casting properties at this specific temperature.The most intense interfacial reaction occurs at 1,520℃,resulting in the formation of the main interfacial reaction products such as Al_(2)O_(3),SiO_(2),and HfO_(2).Additionally,some crystal-like products rich in Si and Hf distribute on the reaction layer.
文摘目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3DL1的mRNA含量。结果通过电泳和实时定量PCR的熔解曲线明确了KIR3DS1和KIR3DL1引物,其特异性好,无杂带。应用TA克隆建立的KIR定量标准品线性关系好,可检测待测物的范围大。应用临床标本可检测出两组标本有显著差异,符合临床预期。结论本文建立的KIR mRNA定量检测方法,可以定量检测活化和抑制性KIR,可以进一步研究不同疾病进展的机制以及预测疾病的临床转归。
