Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HN...Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HNF4A pathogenic mutations exhibit decreased responses to arginine and reduced plasma triglyceride levels,but the mechanisms remain unclear.This study aims to investigate the potential target genes transcriptionally regulated by HNF4A and explore its role in these metabolic pathways.Methods A stable 293T cell line expressing the HNF1A reporter was overexpressed with HNF4A.RNA sequencing(RNA-seq)was performed to analyze transcriptional differences.Transcription factor binding site prediction was then conducted to identify HNF4A binding motifs in the promoter regions of relevant target genes.Results RNA-seq results revealed a significant upregulation of transmembrane 4 L six family member 5(TM4SF5)mRNA in HNF4A-overexpressing cells.Transcription factor binding predictions suggested the presence of five potential HNF4A binding motifs in the TM4SF5 promoter.Finally,we confirmed that the DR1 site in the-57 to-48 region of the TM4SF5 promoter is the key binding motif for HNF4A.Conclusion This study identified TM4SF5 as a target gene of HNF4A and determined the key binding motif involved in its regulation.Given the role of TM4SF5 as an arginine sensor in mTOR signaling activation and triglyceride secretion,which closely aligns with phenotypes observed in MODY1 patients,our findings provide novel insights into the possible mechanisms by which HNF4A regulates triglyceride secretion in the liver and arginine-stimulated insulin secretion in the pancreas.展开更多
木卫六是木星最大的一颗不规则天然卫星,为了获得它的高精度观测位置,天体测量归算过程中各方面都需要考虑,其中特别重要的是参考星表的选择。应用最新公布的星表Gaia Data Release 1(Gaia DR1),同时采用UCAC4星表进行比较,对2015年云...木卫六是木星最大的一颗不规则天然卫星,为了获得它的高精度观测位置,天体测量归算过程中各方面都需要考虑,其中特别重要的是参考星表的选择。应用最新公布的星表Gaia Data Release 1(Gaia DR1),同时采用UCAC4星表进行比较,对2015年云南天文台获得的185个木卫六的观测资料进行处理和归算,结果显示,采用Gaia DR1星表的木卫六的位置测量精度在赤经和赤纬两个方向都约为0.02″,比采用UCAC4的结果有显著提升。展开更多
Two-layer structure consisting of PS/PMMA-DR1 composite film planar waveguide layer on porous silicon cladding layer was fabricated in our experiment. The induced grating based on the third nonlinear optical propertie...Two-layer structure consisting of PS/PMMA-DR1 composite film planar waveguide layer on porous silicon cladding layer was fabricated in our experiment. The induced grating based on the third nonlinear optical properties was formed by interaction of two Nd∶YAG laser beams at 1064nm in the porous silicon/PMMA-DR1 waveguide. The diffraction efficiency of the first order diffracted light is measured to be about 0.2% of the total output.展开更多
We made a comprehensive investigation on the electro-optic（EO） properties of hybrid sol-gel silica film doped with Dispersed Red 1 dye（DR1/SiO2）. An important finding is that the dipoles which don＇t orient along ...We made a comprehensive investigation on the electro-optic（EO） properties of hybrid sol-gel silica film doped with Dispersed Red 1 dye（DR1/SiO2）. An important finding is that the dipoles which don＇t orient along the direction of corona poling field in the films can be instantaneously poled by alternate modulation electrical field. This instant poling effect results in the EO coefficients dependent on the frequency and intensity of alternate modulation electric field, and some experiments have proved it. We also investigated the electro-optic coefficients and thickness of films at different stir temperatures（Ts） of start solution and at different number densities of DR1. Experimentally the DR1 number density was optimized to an order of 1021/cm^3 and a large EO coefficient of γ33= 37 pm/V for DR1/SiO2 film was measured by simple reflection technique at 1 kHz frequencies of modulation electric field. These findings may benefit the practical application of DR1/SiO2 films in the field of EO modulator and EO probing.展开更多
A non-linear optical（NLO） material was synthesized by means of a simple method.This material can be applied in electro-optical（EO） devices,such as EO switches and modulators.The Disperse Red 1（DR1） was doped in ...A non-linear optical（NLO） material was synthesized by means of a simple method.This material can be applied in electro-optical（EO） devices,such as EO switches and modulators.The Disperse Red 1（DR1） was doped in polymethyl-methacrylate-co-glycidyl methacrylate（PMMA-co-GMA） as the NLO active chromophore.The synthesis process and material structure were expressed.The thermal properties,surface morphology and optical characteristics of the material were investigated by means of differential scanning calorimetry（DSC）,thermogravimetric analysis（TGA）,atomic force microscopy（AFM）,and m-lines technique.Using the reflection technique,the value of the electro-optical coefficient γ 33 was measured as 11 pm/V at a wavelength of 1310 nm.An EO switch with nanosecond response based on this material has been fabricated.展开更多
文摘Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HNF4A pathogenic mutations exhibit decreased responses to arginine and reduced plasma triglyceride levels,but the mechanisms remain unclear.This study aims to investigate the potential target genes transcriptionally regulated by HNF4A and explore its role in these metabolic pathways.Methods A stable 293T cell line expressing the HNF1A reporter was overexpressed with HNF4A.RNA sequencing(RNA-seq)was performed to analyze transcriptional differences.Transcription factor binding site prediction was then conducted to identify HNF4A binding motifs in the promoter regions of relevant target genes.Results RNA-seq results revealed a significant upregulation of transmembrane 4 L six family member 5(TM4SF5)mRNA in HNF4A-overexpressing cells.Transcription factor binding predictions suggested the presence of five potential HNF4A binding motifs in the TM4SF5 promoter.Finally,we confirmed that the DR1 site in the-57 to-48 region of the TM4SF5 promoter is the key binding motif for HNF4A.Conclusion This study identified TM4SF5 as a target gene of HNF4A and determined the key binding motif involved in its regulation.Given the role of TM4SF5 as an arginine sensor in mTOR signaling activation and triglyceride secretion,which closely aligns with phenotypes observed in MODY1 patients,our findings provide novel insights into the possible mechanisms by which HNF4A regulates triglyceride secretion in the liver and arginine-stimulated insulin secretion in the pancreas.
基金National Natural Science Foundation of China(60067001) West Glory Project of Chinese Academy of Science(2003XJDX) +1 种基金Excellent Youth Scholar Award Foundation of Xinjiang(XJEDU2004E02)
文摘Two-layer structure consisting of PS/PMMA-DR1 composite film planar waveguide layer on porous silicon cladding layer was fabricated in our experiment. The induced grating based on the third nonlinear optical properties was formed by interaction of two Nd∶YAG laser beams at 1064nm in the porous silicon/PMMA-DR1 waveguide. The diffraction efficiency of the first order diffracted light is measured to be about 0.2% of the total output.
基金Supported by the Chinese University Innovation Program from Ministry of Education of China(No.706017)
文摘We made a comprehensive investigation on the electro-optic（EO） properties of hybrid sol-gel silica film doped with Dispersed Red 1 dye（DR1/SiO2）. An important finding is that the dipoles which don＇t orient along the direction of corona poling field in the films can be instantaneously poled by alternate modulation electrical field. This instant poling effect results in the EO coefficients dependent on the frequency and intensity of alternate modulation electric field, and some experiments have proved it. We also investigated the electro-optic coefficients and thickness of films at different stir temperatures（Ts） of start solution and at different number densities of DR1. Experimentally the DR1 number density was optimized to an order of 1021/cm^3 and a large EO coefficient of γ33= 37 pm/V for DR1/SiO2 film was measured by simple reflection technique at 1 kHz frequencies of modulation electric field. These findings may benefit the practical application of DR1/SiO2 films in the field of EO modulator and EO probing.
基金Supported by the National Natural Science Foundation of China(Nos.61107019,61177027,61077041,60807029)the Science Foundation for Young Scientists of Jilin Province,China(No. 20100174)+1 种基金the Science and Technology Development Plan of Jilin Province,China(No.20110315)the Program for Special Funds of Basic Science & Technology of Jilin University,China(Nos.201103071,201100253,200905005)
文摘A non-linear optical（NLO） material was synthesized by means of a simple method.This material can be applied in electro-optical（EO） devices,such as EO switches and modulators.The Disperse Red 1（DR1） was doped in polymethyl-methacrylate-co-glycidyl methacrylate（PMMA-co-GMA） as the NLO active chromophore.The synthesis process and material structure were expressed.The thermal properties,surface morphology and optical characteristics of the material were investigated by means of differential scanning calorimetry（DSC）,thermogravimetric analysis（TGA）,atomic force microscopy（AFM）,and m-lines technique.Using the reflection technique,the value of the electro-optical coefficient γ 33 was measured as 11 pm/V at a wavelength of 1310 nm.An EO switch with nanosecond response based on this material has been fabricated.
