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Modelling the Biomass Growth and Enzyme Secretion by the White Rot Fungus <i>Phanerochaete Chrysosporium</i>in Presence of A Toxic Pollutant
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作者 Kausik Sen Kannan Pakshirajan Sitangshu Bikas Santra 《Journal of Environmental Protection》 2012年第1期114-119,共6页
The white rot fungus Phanerochaete chrysosporium is well known for its ability to degrade toxic pollutants owing to its efficient extracellular ligninase system. However, biomass growth and enzyme secretion in presenc... The white rot fungus Phanerochaete chrysosporium is well known for its ability to degrade toxic pollutants owing to its efficient extracellular ligninase system. However, biomass growth and enzyme secretion in presence of toxic pollutant is not well understood. In the present study, using the model azo dye Direct Red-80, biomass growth and lignin peroxidase secretion by the fungus was studied during its degradation and a stochastic based model was applied to simulate the behavior of the fungus. Also, glucose concentration in the medium was varied in order to observe its effect on the dye degradation. Results revealed that glucose at an optimum concentration of 10 gL-1 is essential for biomass growth, LiP secretion, as well as the dye decolourization. Modeling the behavior of the fungus with the presence of both glucose and dye has shown significant similarity. 展开更多
关键词 TOXIC POLLUTANT PHANEROCHAETE CHRYSOSPORIUM dr-80 Dye Decolourization Stochastic Model Lignin Peroxidase
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不同浓度碘对体外培养的人甲状腺细胞HLA-DR、CD_(40)、B7.1及Fas表达的影响 被引量:6
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作者 李玉姝 单忠艳 +5 位作者 滕卫平 李静 郭晓临 王立娥 都姝妍 范晨玲 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2002年第6期474-477,共4页
目的 通过研究碘对正常人甲状腺细胞表面免疫相关抗原表达的影响 ,以期在靶细胞水平探讨碘在自身免疫性甲状腺疾病 (AITD)发生发展中的作用机制。方法 传代培养的正常人甲状腺细胞 ,单独以碘化钾刺激或与细胞因子干扰素 (IFN)γ、IFN... 目的 通过研究碘对正常人甲状腺细胞表面免疫相关抗原表达的影响 ,以期在靶细胞水平探讨碘在自身免疫性甲状腺疾病 (AITD)发生发展中的作用机制。方法 传代培养的正常人甲状腺细胞 ,单独以碘化钾刺激或与细胞因子干扰素 (IFN)γ、IFN α共同作用后 ,分析甲状腺细胞表面HLA DR抗原、粘附分子CD4 0 和B7.1分子 (CD80 )及Fas抗原的表达变化。结果 IFN γ能诱导正常人甲状腺细胞表达HLA DR ,碘不影响IFN γ的诱导作用 ;正常人甲状腺细胞表达CD4 0 分子 ,IFN γ能明显促进其表达 ,碘能够协同IFN γ的刺激作用 (P <0 .0 5 ) ;IFN α能刺激甲状腺细胞B7.1的表达 ;碘有增加IFN α诱导的甲状腺细胞B7.1表达的趋势 ;正常人甲状腺细胞表达Fas抗原 ,IFN γ能增强其表达 ,碘不影响甲状腺细胞表面Fas的表达。结论 碘可能通过细胞因子等的作用刺激甲状腺细胞CD4 0 和B7.1的表达 。 展开更多
关键词 自身免疫性甲状腺疾病 体外培养 甲状腺细胞 HLA-DR CD40 B7.1 Fas
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