Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and diabetes.The study was conducted to investigate the antioxidant and ...Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and diabetes.The study was conducted to investigate the antioxidant and anti-lipoxygenase(LOX)properties of the isolated compound 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one from Pistacia chinensis.Methods:LOX assay and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay were performed.Molecular docking studies were conducted using a molecular operating environment.Results:The LOX assay revealed significant inhibitory effects at 0.2µM concentration,with an IC50 value of 37.80µM.The antioxidant effect demonstrated dose-dependency across 5 to 100µg/mL concentrations,reaching 93.09%at 100µg/mL,comparable to ascorbic acid’s 95.43%effect.Molecular docking studies highlighted strong interactions with the lipoxygenase enzyme,presenting an excellent docking score of-10.98 kcal/mol.Conclusion:These findings provide valuable insights into Pistacia chinensis’chemical components and biological effects,reinforcing its traditional medicinal applications.展开更多
To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniqu...To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniques and were identified by spectral analysis. The antioxidant activities of compounds were evaluated by DPPH free radical scavenging assay. Five phenanthrenes and four lignans were obtained from the active fractions ofD. nobile. Their structures were identified as fimbriatone （1）, confusarin （2）, flavanthrinin （3）, 2,5-dihydroxy-4,9-dimethoxyphenanthrene （4）, 3,7-dihydroxy-2,4-dimethoxyphenanthrene （5）, syringaresinol （6）, pinoresinol （7）, medioresinol （8） and lirioresinol-A （9）, respectively. Compounds 2 and 6 exhibited more potent DPPH scavenging activities than vitamin C. All the above compounds were reported from this plant for the first time, and compounds 3, 4 and 9 were reported for the first time from the genus of Dendrobiurn. For all phenanthrenes and lignans, an electron-donating methoxyl group in the ortho position to the phenolic hydroxyl group exhibits enhanced antioxidant activities.展开更多
Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothi...Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)assays,which can measure single electron transfer reactions.Therefore,we attempted to investigate the radical scavenging reaction of each antioxidant in the DPPH and ABTS assays by using electron spin resonance(ESR),and to study an analytical method that can eliminate other than that factor.Results:Singular value decomposition(SVD)of the spectra that were obtained by ESR measurements was used to separate the components and to determine the detailed changes of each component.We calculated the EC50 from the changes in the spectra of the first and second components owing to the change in the antioxidant concentration in the DPPH and ABTS assays to identify which component contributed more to the radical scavenging reaction.The results demonstrated a positive correlation(R2=0.8806)between the EC50s of the first component of each of the DPPH and ABTS assays.In this study,common spectral changes were observed for all compounds with radical scavenging activity,so the first component was considered to be radical scavenging activity.Conclusions:Through this study,we could calculate the EC50 that significantly contributed to the radical scavenging reaction by decomposing the spectra that were obtained from ESR measurements of the reactions by the DPPH and ABTS assays into specific values.展开更多
Albizia coriaria Welw ex.Oliver(hereafter A.coriaria)is a revered medicinal tree whose organs are used by indigenous African societies for managing various ailments.Herein,we assessed the secondary metabolites,total p...Albizia coriaria Welw ex.Oliver(hereafter A.coriaria)is a revered medicinal tree whose organs are used by indigenous African societies for managing various ailments.Herein,we assessed the secondary metabolites,total polyphenolics,free radical scavenging and antimicrobial activities of A.coriaria flowers.The dry powdered sample was extracted serially with ethyl acetate,ethanol and distilled water.Results of classical phytochemical screening showed that saponins,phenols,alkaloids,flavonoids,tannins and terpenes were the dominant phytochemicals.The highest total polyphenolic content(10.37±0.02 mg QE/g DW and 72.09±0.09 mg GAE/g DW)and free radical scavenging potential(IC50=24.23±0.04 mg/mL)were for the ethyl acetate extract.It also had a higher bacteriostatic activity than the ethanolic extract against Pseudomonas aeruginosa,Salmonella typhi,Escherichia coli and Staphylococcus aureus with diameters of inhibition ranging from 7.00±0.00 to 22.00±1.73 mm.The aqueous extract showed no appreciable antibacterial effect on the tested microorganisms.This is the first report which support the use of floral extracts of this species in the management of ailments in African traditional medicine.展开更多
Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in...Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in the Cameroonian savannah’s region to protect foodstuffs and to cure degenerative diseases. Results indicated that theses extracts contain terpenoids, phenolic and flavonoid compounds. Except the n-hexane extract, all of the obtained extracts exhibit antioxidant activities with the ethanol extract being the most effective with an inhibition percentage of 85.668% ± 1.233% and an inhibition concentration (IC<sub>50</sub>) of 58 μg/ml (R<sup>2</sup> = 0.987, P < 0.01) at a concentration of 100 mg/ml. Chromatographic separation on silica gel of the ethanol extract led to the isolation of a pure organic compound which was characterized as 7-hydroxy-5,6,4'-trimethox- yflavone by extensive 1D and 2D NMR spectroscopy, a flavonoid exhibiting antioxidative activity with an inhibitory percentage of 25.506% ± 0.205% and inhibition concentration (IC<sub>50</sub>) of 221 μg/ml (R<sup>2</sup> = 0.966, P < 0.01). This is the first time that 7-hydroxy-5,6,4'-trimethoxyflavone is being isolated from L. rugosa and its antioxidant activity evaluated.展开更多
Arsenic has a suppressive influence on spermatogenesis and induces impairment in male reproductive system due to oxidative stress. The present study was aimed to test the arsenic induced toxicity and protection by Chl...Arsenic has a suppressive influence on spermatogenesis and induces impairment in male reproductive system due to oxidative stress. The present study was aimed to test the arsenic induced toxicity and protection by Chlorophytum borivilianum. The effect of sodium arsenite （4 mg/（kg body weight （bw）.day）） via double distilled water without or with C. borivilianum （800 mg/（kg bw.day）） was evaluated in Swiss albino mice for 30 days. The radical scavenging activity of the aqueous C. borivilianum root extract was measured using DPPH （1,1-diphenyl-2-picryl hydrayzyl） radical. Qualitative assessment of various cell types in the testis, sperm count and motility, testicular activity of lipid peroxidation （LPO）, reduced glutathione （GSH）, acid and alkaline phosphatase, cholesterol and serum testosterone were monitored. Arsenic treatment showed a significant increase in LPO, acid and alkaline phosphatase, cholesterol and decrease in sperm count, sperm motility, GSH and serum testosterone. Combined treatment showed significant decrease in LPO, acid and alkaline phosphatase, cholesterol and elevation in sperm count, sperm motility, GSH and serum testosterone. Testicular histopathology showed that C. borivilianum had reduced degeneration of germ cell in the seminiferous tubules and loss of sperms induced by arsenic intoxication. The results thus led us to conclude that administration of C. borivilianum root extract is found to be protective against arsenic induced toxicity.展开更多
文摘Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and diabetes.The study was conducted to investigate the antioxidant and anti-lipoxygenase(LOX)properties of the isolated compound 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one from Pistacia chinensis.Methods:LOX assay and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay were performed.Molecular docking studies were conducted using a molecular operating environment.Results:The LOX assay revealed significant inhibitory effects at 0.2µM concentration,with an IC50 value of 37.80µM.The antioxidant effect demonstrated dose-dependency across 5 to 100µg/mL concentrations,reaching 93.09%at 100µg/mL,comparable to ascorbic acid’s 95.43%effect.Molecular docking studies highlighted strong interactions with the lipoxygenase enzyme,presenting an excellent docking score of-10.98 kcal/mol.Conclusion:These findings provide valuable insights into Pistacia chinensis’chemical components and biological effects,reinforcing its traditional medicinal applications.
文摘To study the antioxidant constituents from the stems of Dendrobium nobile, and to discuss theft structure-activity relationship. Compounds were isolated from a 60% ethanolic extract by various chromatographic techniques and were identified by spectral analysis. The antioxidant activities of compounds were evaluated by DPPH free radical scavenging assay. Five phenanthrenes and four lignans were obtained from the active fractions ofD. nobile. Their structures were identified as fimbriatone （1）, confusarin （2）, flavanthrinin （3）, 2,5-dihydroxy-4,9-dimethoxyphenanthrene （4）, 3,7-dihydroxy-2,4-dimethoxyphenanthrene （5）, syringaresinol （6）, pinoresinol （7）, medioresinol （8） and lirioresinol-A （9）, respectively. Compounds 2 and 6 exhibited more potent DPPH scavenging activities than vitamin C. All the above compounds were reported from this plant for the first time, and compounds 3, 4 and 9 were reported for the first time from the genus of Dendrobiurn. For all phenanthrenes and lignans, an electron-donating methoxyl group in the ortho position to the phenolic hydroxyl group exhibits enhanced antioxidant activities.
基金supported by JSPS KAKENHI,Japan Grant Number 17K05366.
文摘Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)assays,which can measure single electron transfer reactions.Therefore,we attempted to investigate the radical scavenging reaction of each antioxidant in the DPPH and ABTS assays by using electron spin resonance(ESR),and to study an analytical method that can eliminate other than that factor.Results:Singular value decomposition(SVD)of the spectra that were obtained by ESR measurements was used to separate the components and to determine the detailed changes of each component.We calculated the EC50 from the changes in the spectra of the first and second components owing to the change in the antioxidant concentration in the DPPH and ABTS assays to identify which component contributed more to the radical scavenging reaction.The results demonstrated a positive correlation(R2=0.8806)between the EC50s of the first component of each of the DPPH and ABTS assays.In this study,common spectral changes were observed for all compounds with radical scavenging activity,so the first component was considered to be radical scavenging activity.Conclusions:Through this study,we could calculate the EC50 that significantly contributed to the radical scavenging reaction by decomposing the spectra that were obtained from ESR measurements of the reactions by the DPPH and ABTS assays into specific values.
文摘Albizia coriaria Welw ex.Oliver(hereafter A.coriaria)is a revered medicinal tree whose organs are used by indigenous African societies for managing various ailments.Herein,we assessed the secondary metabolites,total polyphenolics,free radical scavenging and antimicrobial activities of A.coriaria flowers.The dry powdered sample was extracted serially with ethyl acetate,ethanol and distilled water.Results of classical phytochemical screening showed that saponins,phenols,alkaloids,flavonoids,tannins and terpenes were the dominant phytochemicals.The highest total polyphenolic content(10.37±0.02 mg QE/g DW and 72.09±0.09 mg GAE/g DW)and free radical scavenging potential(IC50=24.23±0.04 mg/mL)were for the ethyl acetate extract.It also had a higher bacteriostatic activity than the ethanolic extract against Pseudomonas aeruginosa,Salmonella typhi,Escherichia coli and Staphylococcus aureus with diameters of inhibition ranging from 7.00±0.00 to 22.00±1.73 mm.The aqueous extract showed no appreciable antibacterial effect on the tested microorganisms.This is the first report which support the use of floral extracts of this species in the management of ailments in African traditional medicine.
文摘Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in the Cameroonian savannah’s region to protect foodstuffs and to cure degenerative diseases. Results indicated that theses extracts contain terpenoids, phenolic and flavonoid compounds. Except the n-hexane extract, all of the obtained extracts exhibit antioxidant activities with the ethanol extract being the most effective with an inhibition percentage of 85.668% ± 1.233% and an inhibition concentration (IC<sub>50</sub>) of 58 μg/ml (R<sup>2</sup> = 0.987, P < 0.01) at a concentration of 100 mg/ml. Chromatographic separation on silica gel of the ethanol extract led to the isolation of a pure organic compound which was characterized as 7-hydroxy-5,6,4'-trimethox- yflavone by extensive 1D and 2D NMR spectroscopy, a flavonoid exhibiting antioxidative activity with an inhibitory percentage of 25.506% ± 0.205% and inhibition concentration (IC<sub>50</sub>) of 221 μg/ml (R<sup>2</sup> = 0.966, P < 0.01). This is the first time that 7-hydroxy-5,6,4'-trimethoxyflavone is being isolated from L. rugosa and its antioxidant activity evaluated.
基金the UGC New Delhi for providing financial assistance to Garima Sharma as Junior Research Fellow,Letter No. F-414/NET/RES/JRF-699
文摘Arsenic has a suppressive influence on spermatogenesis and induces impairment in male reproductive system due to oxidative stress. The present study was aimed to test the arsenic induced toxicity and protection by Chlorophytum borivilianum. The effect of sodium arsenite （4 mg/（kg body weight （bw）.day）） via double distilled water without or with C. borivilianum （800 mg/（kg bw.day）） was evaluated in Swiss albino mice for 30 days. The radical scavenging activity of the aqueous C. borivilianum root extract was measured using DPPH （1,1-diphenyl-2-picryl hydrayzyl） radical. Qualitative assessment of various cell types in the testis, sperm count and motility, testicular activity of lipid peroxidation （LPO）, reduced glutathione （GSH）, acid and alkaline phosphatase, cholesterol and serum testosterone were monitored. Arsenic treatment showed a significant increase in LPO, acid and alkaline phosphatase, cholesterol and decrease in sperm count, sperm motility, GSH and serum testosterone. Combined treatment showed significant decrease in LPO, acid and alkaline phosphatase, cholesterol and elevation in sperm count, sperm motility, GSH and serum testosterone. Testicular histopathology showed that C. borivilianum had reduced degeneration of germ cell in the seminiferous tubules and loss of sperms induced by arsenic intoxication. The results thus led us to conclude that administration of C. borivilianum root extract is found to be protective against arsenic induced toxicity.
