Background In July 2024,the European Union's Ecodesign for Sustainable Products Regulation,or ESPR,officially came into effect.This regulation mandates that products entering the EU market must use a Digital Produ...Background In July 2024,the European Union's Ecodesign for Sustainable Products Regulation,or ESPR,officially came into effect.This regulation mandates that products entering the EU market must use a Digital Product Passport,known as DPP,to disclose their compliance certifications,environmental impact,and supply chain information.Failure to comply may result in exclusion from the EU market.展开更多
Lysosomal dysfunction has been implicated in the progression of colon adenocarcinoma(COAD),yet the prognostic significance and therapeutic potential of lysosome-related genes(LRGs)remain underexplored.In this study,we...Lysosomal dysfunction has been implicated in the progression of colon adenocarcinoma(COAD),yet the prognostic significance and therapeutic potential of lysosome-related genes(LRGs)remain underexplored.In this study,we construct a 6-LRG-based prognostic risk stratification model(DPP7,ADAM8,CD1B,LRP2,ATP6V1C2,and PLAAT3)by integrating LASSO and Cox regression analyses.Stratifying patients based on median risk scores,we demonstrate that high-risk patients exhibit significantly worse clinical outcomes across the TCGA cohort and five independent GEO datasets.Furthermore,this panel outperforms 136 previously published models in terms of predictive accuracy for 1-,3-,and 5-year survival rates.Validation multiplex immunofluorescence using an in-house tissue microarray cohort confirms that the 6-LRG signature serves as an independent prognostic factor.Additionally,high-risk patients exhibit distinct immunosuppressive tumor microenvironment and aggressive malignancy characteristics.Functional depletion of DPP7 significantly inhibits tumor cell proliferation,migration,and metastasis in both in vitro and in vivo settings.Moreover,DPP7 silencing attenuates epithelialemesenchymal transition,as evidenced by the upregulation of E-cadherin and downregulation of N-cadherin,Vimentin,and Snail.In conclusion,this study establishes an LRG-based model for COAD prognostic prediction and nominates DPP7 as a promising therapeutic target for COAD treatment.展开更多
目的探索慢性失眠、抑郁状态伴失眠患者中血清S100钙结合蛋白β(S100 calcium binding protein beta,S100β)和二肽基肽酶4(Dipeptidyl peptidase-4,DPP-4)水平的改变,并评估其作为判断慢性失眠、抑郁状态伴失眠生物标志物的临床价值。...目的探索慢性失眠、抑郁状态伴失眠患者中血清S100钙结合蛋白β(S100 calcium binding protein beta,S100β)和二肽基肽酶4(Dipeptidyl peptidase-4,DPP-4)水平的改变,并评估其作为判断慢性失眠、抑郁状态伴失眠生物标志物的临床价值。方法纳入南华大学附属南华医院的慢性失眠、抑郁状态伴失眠患者采取临床诊断和抑郁筛查自测量表(Patient Health Questionnaire,PHQ-9)和失眠严重程度指数量表(Insomnia Severity Index,ISI)方式,将被试者分为失眠组(n=64)和抑郁状态伴失眠组(n=110),同期选取心理评估正常的健康体检者作为正常组(n=64)。采用ELISA检测三组的血清S100β和DPP-4水平,比较三组在基线期的差异,并纵向比较患者组治疗前后指标变化;采用皮尔逊相关分析探索血清指标与抑郁和失眠症状的相关性;通过ROC曲线评估两项指标对失眠组、抑郁状态伴失眠组的诊断价值。结果三组间一般资料比较无统计学差异;三组的血清S100β和DPP-4水平存在明显差异,且呈现为正常组<失眠组<抑郁状态伴失眠组(P均<0.05),且患者组的血清S100β和DPP-4水平与PHQ-9和ISI评分呈正相关(r=0.209~0.479,P<0.05);ROC曲线模型检验提示慢性失眠患者S100β曲线下面积为0.746,DPP-4的曲线下面积为0.785;抑郁状态伴失眠的患者中S100β曲线下面积为0.651,DPP-4的曲线下面积为0.636。患者组予以改善睡眠、抗抑郁等治疗后,两组PHQ-9和/或ISI评分明显减低,S100β和DPP-4(P<0.001)水平明显降低。结论慢性失眠、抑郁状态伴失眠患者的血清S100β、DPP-4水平升高;血清S100β和DPP-4水平有望成为判断慢性失眠、抑郁状态伴失眠的潜在生物标志物。展开更多
目的 分析血清果蝇母性DPP同源物(serum small mother against decapentaplegic,Smad)2、Smad3与冠状动脉粥样硬化性心脏病(冠心病)患者心脏猝死的相关性。方法 选取2018年3月至2020年6月无锡市人民医院收治的住院接受治疗的冠心病患者...目的 分析血清果蝇母性DPP同源物(serum small mother against decapentaplegic,Smad)2、Smad3与冠状动脉粥样硬化性心脏病(冠心病)患者心脏猝死的相关性。方法 选取2018年3月至2020年6月无锡市人民医院收治的住院接受治疗的冠心病患者134例为研究对象,随访1年,根据冠心病患者有无发生心脏猝死分为猝死组(21例)和未猝死组(113例)。采用酶联免疫吸附试验(ELISA)法检测患者血清中Smad2、Smad3浓度。采用动态心电图仪详细记录患者QT间期、PR间期、QRS波持续时间。采用受试者工作特征曲线(receiver operating characteristic curve,ROC)评价QRS波持续时间联合Smad2、Smad3对冠心病患者心脏猝死的诊断价值。应用二元Logistic回归分析对影响冠心病患者心脏猝死的危险因素进行分析。结果 与未猝死组相比,猝死组患者QRS波持续时间[(116.74±14.58)ms vs.(88.42±10.67)ms]、血清Smad2[(286.42±38.71)μg/L vs.(164.93±22.28)μg/L]、Smad3[(335.18±50.06)μg/L vs.(190.25±29.72)μg/L]浓度较高,差异有统计学意义(P<0.05)。ROC分析显示,QRS波持续时间、血清Smad2和Smad3浓度诊断冠心病患者心脏猝死的曲线下面积(area under the curve,AUC)分别为0.742、0.825、0.842;QRS波持续时间联合Smad2、Smad3诊断冠心病患者心脏猝死的AUC为0.893,其敏感度、特异度分别为81.00%、93.80%。QRS波持续时间、Smad2、Smad3是冠心病患者心脏猝死危险因素(P<0.05)。结论 动态心电图联合血清Smad2、Smad3浓度检测对预测冠心病患者心脏猝死有一定价值。展开更多
Aim： To investigate the role of a novel dipeptidyl peptidase 8 transcript variant （DPP8-v3） gene in testis development and/or spermatogenesis. Methods： A human testis cDNA microarray was hybridized with mRNA of hu...Aim： To investigate the role of a novel dipeptidyl peptidase 8 transcript variant （DPP8-v3） gene in testis development and/or spermatogenesis. Methods： A human testis cDNA microarray was hybridized with mRNA of human adult and fetal testes. Differentially expressed clones were sequenced and characterized and their expression was analyzed by real-time reverse transcription polymerase chain reaction （RT-PCR） and Southern-blot analysis. Results： A new transcript variant of the human dipeptidyl peptidase （DPP8）, exhibiting a 5-fold higher expression level in human adult than that in fetal testes, was cloned and was named DPP8 variant 3 （DPP8-v3）. The full-length sequence of DPP8-v3 was 3,030 bp, encoding a protein of 898 amino acids. Conclusion： DPPS-v3 is a novel human DPP8 transcript variant highly expressed in the adult testis. Similar to DPPIV, DPP8-v3 may play a key role in the immunoregulation of testes and accordingly may influence spermatogenesis and male fertility. （Asian J Androl 2005 Sep; 7： 245-255）展开更多
文摘Background In July 2024,the European Union's Ecodesign for Sustainable Products Regulation,or ESPR,officially came into effect.This regulation mandates that products entering the EU market must use a Digital Product Passport,known as DPP,to disclose their compliance certifications,environmental impact,and supply chain information.Failure to comply may result in exclusion from the EU market.
基金supported by the Natural Science Foundation of Shandong Province(ZR2021MH110,ZR2020MH257,ZR2020MH323)the National Natural Science Foundation of China(82172339 and 82272410)+1 种基金Major Scientific and Technological Innovation Project of Shandong Province(2021CXGC010603 and 2021CXGC011105)Taishan Scholar Program of Shandong Province(tstp20221156 and tsqn202306346).
文摘Lysosomal dysfunction has been implicated in the progression of colon adenocarcinoma(COAD),yet the prognostic significance and therapeutic potential of lysosome-related genes(LRGs)remain underexplored.In this study,we construct a 6-LRG-based prognostic risk stratification model(DPP7,ADAM8,CD1B,LRP2,ATP6V1C2,and PLAAT3)by integrating LASSO and Cox regression analyses.Stratifying patients based on median risk scores,we demonstrate that high-risk patients exhibit significantly worse clinical outcomes across the TCGA cohort and five independent GEO datasets.Furthermore,this panel outperforms 136 previously published models in terms of predictive accuracy for 1-,3-,and 5-year survival rates.Validation multiplex immunofluorescence using an in-house tissue microarray cohort confirms that the 6-LRG signature serves as an independent prognostic factor.Additionally,high-risk patients exhibit distinct immunosuppressive tumor microenvironment and aggressive malignancy characteristics.Functional depletion of DPP7 significantly inhibits tumor cell proliferation,migration,and metastasis in both in vitro and in vivo settings.Moreover,DPP7 silencing attenuates epithelialemesenchymal transition,as evidenced by the upregulation of E-cadherin and downregulation of N-cadherin,Vimentin,and Snail.In conclusion,this study establishes an LRG-based model for COAD prognostic prediction and nominates DPP7 as a promising therapeutic target for COAD treatment.
文摘目的探索慢性失眠、抑郁状态伴失眠患者中血清S100钙结合蛋白β(S100 calcium binding protein beta,S100β)和二肽基肽酶4(Dipeptidyl peptidase-4,DPP-4)水平的改变,并评估其作为判断慢性失眠、抑郁状态伴失眠生物标志物的临床价值。方法纳入南华大学附属南华医院的慢性失眠、抑郁状态伴失眠患者采取临床诊断和抑郁筛查自测量表(Patient Health Questionnaire,PHQ-9)和失眠严重程度指数量表(Insomnia Severity Index,ISI)方式,将被试者分为失眠组(n=64)和抑郁状态伴失眠组(n=110),同期选取心理评估正常的健康体检者作为正常组(n=64)。采用ELISA检测三组的血清S100β和DPP-4水平,比较三组在基线期的差异,并纵向比较患者组治疗前后指标变化;采用皮尔逊相关分析探索血清指标与抑郁和失眠症状的相关性;通过ROC曲线评估两项指标对失眠组、抑郁状态伴失眠组的诊断价值。结果三组间一般资料比较无统计学差异;三组的血清S100β和DPP-4水平存在明显差异,且呈现为正常组<失眠组<抑郁状态伴失眠组(P均<0.05),且患者组的血清S100β和DPP-4水平与PHQ-9和ISI评分呈正相关(r=0.209~0.479,P<0.05);ROC曲线模型检验提示慢性失眠患者S100β曲线下面积为0.746,DPP-4的曲线下面积为0.785;抑郁状态伴失眠的患者中S100β曲线下面积为0.651,DPP-4的曲线下面积为0.636。患者组予以改善睡眠、抗抑郁等治疗后,两组PHQ-9和/或ISI评分明显减低,S100β和DPP-4(P<0.001)水平明显降低。结论慢性失眠、抑郁状态伴失眠患者的血清S100β、DPP-4水平升高;血清S100β和DPP-4水平有望成为判断慢性失眠、抑郁状态伴失眠的潜在生物标志物。
文摘目的 分析血清果蝇母性DPP同源物(serum small mother against decapentaplegic,Smad)2、Smad3与冠状动脉粥样硬化性心脏病(冠心病)患者心脏猝死的相关性。方法 选取2018年3月至2020年6月无锡市人民医院收治的住院接受治疗的冠心病患者134例为研究对象,随访1年,根据冠心病患者有无发生心脏猝死分为猝死组(21例)和未猝死组(113例)。采用酶联免疫吸附试验(ELISA)法检测患者血清中Smad2、Smad3浓度。采用动态心电图仪详细记录患者QT间期、PR间期、QRS波持续时间。采用受试者工作特征曲线(receiver operating characteristic curve,ROC)评价QRS波持续时间联合Smad2、Smad3对冠心病患者心脏猝死的诊断价值。应用二元Logistic回归分析对影响冠心病患者心脏猝死的危险因素进行分析。结果 与未猝死组相比,猝死组患者QRS波持续时间[(116.74±14.58)ms vs.(88.42±10.67)ms]、血清Smad2[(286.42±38.71)μg/L vs.(164.93±22.28)μg/L]、Smad3[(335.18±50.06)μg/L vs.(190.25±29.72)μg/L]浓度较高,差异有统计学意义(P<0.05)。ROC分析显示,QRS波持续时间、血清Smad2和Smad3浓度诊断冠心病患者心脏猝死的曲线下面积(area under the curve,AUC)分别为0.742、0.825、0.842;QRS波持续时间联合Smad2、Smad3诊断冠心病患者心脏猝死的AUC为0.893,其敏感度、特异度分别为81.00%、93.80%。QRS波持续时间、Smad2、Smad3是冠心病患者心脏猝死危险因素(P<0.05)。结论 动态心电图联合血清Smad2、Smad3浓度检测对预测冠心病患者心脏猝死有一定价值。
文摘Aim： To investigate the role of a novel dipeptidyl peptidase 8 transcript variant （DPP8-v3） gene in testis development and/or spermatogenesis. Methods： A human testis cDNA microarray was hybridized with mRNA of human adult and fetal testes. Differentially expressed clones were sequenced and characterized and their expression was analyzed by real-time reverse transcription polymerase chain reaction （RT-PCR） and Southern-blot analysis. Results： A new transcript variant of the human dipeptidyl peptidase （DPP8）, exhibiting a 5-fold higher expression level in human adult than that in fetal testes, was cloned and was named DPP8 variant 3 （DPP8-v3）. The full-length sequence of DPP8-v3 was 3,030 bp, encoding a protein of 898 amino acids. Conclusion： DPPS-v3 is a novel human DPP8 transcript variant highly expressed in the adult testis. Similar to DPPIV, DPP8-v3 may play a key role in the immunoregulation of testes and accordingly may influence spermatogenesis and male fertility. （Asian J Androl 2005 Sep; 7： 245-255）
