Doubled haploid(DH)technology is an efficient method used in commercial maize breeding.Chromosome doubling is a vital step of DH technology;however,the underlying processes regulating chromosome doubling of haploid is...Doubled haploid(DH)technology is an efficient method used in commercial maize breeding.Chromosome doubling is a vital step of DH technology;however,the underlying processes regulating chromosome doubling of haploid is still not well understood,which is key to optimize the technology.In this study,the immature haploid embryos of the maize inbred line Zheng58 treated with amiprophos-methyl(APM)or colchicine were used to analyze transcriptomic and metabolomic changes,75 and 60 differential expressed metabolites(DEMs)were identified between control treatment,respectively.Most differentially expressed genes(DEGs)related to artificial chromosome doubling were down regulated;these were mainly involved in mitosis process.Both DEMs and DEGs co-expression analyses showed that,compared to controls,zeatin biosynthesis and cofactor and vitamin metabolism were significantly enriched in both APM and colchicine treatments.In a parallel experiment,exogenous vitamins including thiamine,nicotinic acid,vitamin B6,or trans-zeatin were added to colchicine treatment;there were synergistic effects between vitamins or zeatin and colchicine in haploid artificial chromosome doubling.These results provide novel insights in exploring the molecular responses to antimitotic reagents at both the transcriptomic and metabolomic levels.In addition,the application efficiency of haploid breeding will be greatly improved by the key factors for artificial chromosome doubling.展开更多
Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupunctur...Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.展开更多
Water transport at the root/soil interface of 1 year old Pinus sylvestris Linn. var. sylvestriformis (Takenouchi) Cheng et C. D. Chu seedlings under CO 2 doubling was studied by measuring soil electric conductanc...Water transport at the root/soil interface of 1 year old Pinus sylvestris Linn. var. sylvestriformis (Takenouchi) Cheng et C. D. Chu seedlings under CO 2 doubling was studied by measuring soil electric conductance to survey soil water profiles and comparing it with root distribution surveyed by soil coring and root harvesting in Changbai Mountain in 1999. The results were: (1) The profiles of soil water content were adjusted by root activity. The water content of the soil layer with abundant roots was higher. (2) When CO 2 concentration was doubled, water transport was more active at the root/soil interface and the roots were distributed into deeper layer. It was shown in this work that the method of measuring electric conductance is an inexpensive, non_destructive and relatively sensitive way for underground water transport process.展开更多
Potential changes in precipitation extremes in July–August over China in response to CO 2 doubling are analyzed based on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （...Potential changes in precipitation extremes in July–August over China in response to CO 2 doubling are analyzed based on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （20C3M） experiment and the 1% per year CO 2 increase experiment （to doubling） （1pctto2x） of phase 3 of the Coupled Model Inter-comparison Project （CMIP3）. Evaluation of the models’ performance in simulating the mean state shows that the majority of models fairly reproduce the broad spatial pattern of observed precipitation. However, all the models underestimate extreme precipitation by ～50%. The spread among the models over the Tibetan Plateau is ～2–3 times larger than that over the other areas. Models with higher resolution generally perform better than those with lower resolutions in terms of spatial pattern and precipitation amount. Under the 1pctto2x scenario, the ratio between the absolute value of MME extreme precipitation change and model spread is larger than that of total precipitation, indicating a relatively robust change of extremes. The change of extreme precipitation is more homogeneous than the total precipitation. Analysis on the output of Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 （GFDL-CM2.1） indicates that the spatially consistent increase of surface temperature and water vapor content contribute to the large increase of extreme precipitation over contiguous China, which follows the Clausius–Clapeyron relationship. Whereas, the meridionally tri-polar pattern of mean precipitation change over eastern China is dominated by the change of water vapor convergence, which is determined by the response of monsoon circulation to global warming.展开更多
This is the second part of the authors’ analysis on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （20C3M） experiment and 1% per year CO 2 increase experiment （to doub...This is the second part of the authors’ analysis on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （20C3M） experiment and 1% per year CO 2 increase experiment （to doubling） （1pctto2x） of phase 3 of the Coupled Model Inter-comparison Project （CMIP3）. The study focuses on the potential changes of July–August temperature extremes over China. The pattern correlation coefficients of the simulated temperature with the observations are 0.6–0.9, which are higher than the results for precipitation. However, most models have cold bias compared to observation, with a larger cold bias over western China （5°C） than over eastern China （2°C）. The multi-model ensemble （MME） exhibits a significant increase of temperature under the 1pctto2x scenario. The amplitude of the MME warming shows a northwest–southeast decreasing gradient. The warming spread among the models （～1°C– 2°C） is less than MME warming （～2°C–4°C）, indicating a relatively robust temperature change under CO 2 doubling. Further analysis of Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 （GFDL-CM2.1） simulations suggests that the warming pattern may be related to heat transport by summer monsoons. The contrast of cloud effects also has contributions. The different vertical structures of warming over northwestern China and southeastern China may be attributed to the different natures of vertical circulations. The deep, moist convection over southeastern China is an effective mechanism for ＂transporting＂ the warming upward, leading to more upper-level warming. In northwestern China, the warming is more surface-orientated, possibly due to the shallow, dry convection.展开更多
AIM To evaluate the efficacy of doubling time(DT) of gastrointestinal submucosal tumors(GIST).METHODS From April 1987 through November 2012, a total of 323 patients were given a final histopathological diagnosis of GI...AIM To evaluate the efficacy of doubling time(DT) of gastrointestinal submucosal tumors(GIST).METHODS From April 1987 through November 2012, a total of 323 patients were given a final histopathological diagnosis of GISTs on surgical resection or endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA) in Kitasato University East Hospital or Kitasato University Hospital. We studied 53 of these patients(34 with resected tumors and 19 with unresected tumors) whose tumors could be measured on EUS on at least two successive occasions. The histopathological diagnosis was GIST in 34 patients, leiomyoma in 5, schwannoma in 3, ectopic pancreas in 1, hamartoma in 1, cyst in 1, Brunner's adenoma in 1, and spindle-cell tumor in 7. We retrospectively calculated the DT of GISTs on the basis of the time course of EUS findings to estimate the growth rate of such tumors.RESULTS The DT was 17.2 mo for GIST, as compared with 231.2 mo for leiomyoma, 104.7 mo for schwannoma, 274.9mo for ectopic pancreas, 61.2 mo for hamartoma, 49.0 mo for cyst, and 134.7 mo for Brunner's adenoma. The GISTs were divided into risk classes on the basis of tumor diameters and mitotic figures(Fletcher's classification). The classification was extremely low risk or low risk in 28 patients, intermediate risk in 3, and high risk in 3. DT of GIST according to risk was 24.0 mo for extremely low-risk plus low-risk GIST, 17.1 mo for intermediate-risk GIST, and 3.9 mo for high-risk GIST. DT of GIST was significantly shorter than that of leiomyoma plus schwannoma(P < 0.05), and DT of high-risk GIST was significantly shorter than that of extremely low-risk plus low-risk GIST(P < 0.05).CONCLUSION For GIST, a higher risk grade was associated with a significantly shorter DT. Small SMTs should initially be followed up within 6 mo after detection.展开更多
Based on simulations of the IPCC 20C3M and SRES A1B experiments in ten coupled models, the Asian summer mon-soon (ASM) response to CO2 doubling and the different responses among models are examined. Nine models show t...Based on simulations of the IPCC 20C3M and SRES A1B experiments in ten coupled models, the Asian summer mon-soon (ASM) response to CO2 doubling and the different responses among models are examined. Nine models show the similar results that the weakening of land-ocean thermal contrast caused by the CO2 doubling contributes to a weaker large-scale ASM circulation. Further analysis in this study also shows that the major ASM components,such as the Somali cross-equatorial flow,the low level India-South China Sea monsoon trough,and the upper level tropical easterly jet stream, weaken as CO2 doubles.However,the ASM rainfall increases as a result of the increased moisture from the warmer Indian Ocean and the South China Sea, and the enhanced northward moisture transport over the ASM region.For the response of enhanced northward moisture transport over South Asia, the positive contribution of moisture content increase in the Indian Ocean is dominant and the negative contribution of the weaker mon-soon circulation is secondary at 850 hPa,but both have positive contribution to the total moisture transport along the East China coast.The paradox of the weaker ASM circulation and the increasing precipitation in CO2 doubling is confirmed.It is found that strengthening of northward moisture transport could intensify the precipitation and atmospheric heat source over the north Arabian Sea and East China,and result in enhanced southwesterly at 850 hPa as global warming occurs.All ten models show significant enhanced southwesterly response over the north Arabian Sea,and six of them show enhanced southwesterly response along the East China coast.展开更多
Objective:Although the effect of decitabine on myelodysplastic syndrome(MDS)has been demonstrated,merely a proportion of patients respond to therapy,and no well-recognized predictors have been identified.This study wa...Objective:Although the effect of decitabine on myelodysplastic syndrome(MDS)has been demonstrated,merely a proportion of patients respond to therapy,and no well-recognized predictors have been identified.This study was conducted to investigate the effectiveness of decitabine in real-world clinical practice,and determine the predictive factors of response and overall survival(OS)in MDS patients.展开更多
Studies of barley and maize indicate that chromosome doubling occurs via nuclear fusion during an early stage of microspore embryogenesis, but the time and mechanism by which chromosome doubling occurs in bread wheat ...Studies of barley and maize indicate that chromosome doubling occurs via nuclear fusion during an early stage of microspore embryogenesis, but the time and mechanism by which chromosome doubling occurs in bread wheat (Triticum aestivum) remains undetermined. The purpose of this study was to determine the relative time during induction culture when chromosome doubling may occur in wheat, and to identify early indicators for doubled haploid microspores. Microspore nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) and observed under a fluorescent microscope on the day of isolation, three days after isolation, and six days after isolation. The change in the percentage of microspores containing a single small nucleus, two small nuclei, a single enlarged nucleus, and three or more nuclei was then tracked throughout the six-day period. Ploidy levels were estimated by determining the cross-sectional area and number of nucleoli in microspores containing small and large nuclei then comparing the results of each respective cell-type. The percentage of microspores containing enlarged nuclei increased throughout the six-day test period, and the percentage of binucleated microspores containing small nuclei decreased. Comparison of the changes in average percentage of microspores containing a single small nucleus, binucleated microspores, microspores containing a single large nucleus, and multinucleate microspores on days 0, 3, and 6 indicates that nuclei classified as “small” are likely haploids and nuclei classified as “large” are doubled haploids. The percentage of microspores with enlarged nucleus (nuclei) during the first six days of induction culture could be used as an early indicator for the frequency of chromosome doubling in wheat microspore culture.展开更多
An improved recursive doubling algorithm for solving linear recurrence R <n,1>is given,whose parallel time complexity is (τ++τ.) logn when n processors are available,achieving the lower bound in array processo...An improved recursive doubling algorithm for solving linear recurrence R <n,1>is given,whose parallel time complexity is (τ++τ.) logn when n processors are available,achieving the lower bound in array processor type computation.展开更多
Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts.Extensive studies have shown genetic,transcriptomic,and epigenetic dynamics upon polyploidization in...Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts.Extensive studies have shown genetic,transcriptomic,and epigenetic dynamics upon polyploidization in multiple plant species.However,few studies have unveiled those alternations imposed only by ploidy level,without any interference from heterozygosity.Cultivated potato is highly heterozygous.Thus,in this study,we developed two homozygous autotetraploid lines and one homozygous diploid line in parallel from a homozygous diploid potato.We confirmed their ploidy levels using chloroplast counting and karyotyping.Oligo-FISH and genome re-sequencing validated that these potato lines are nearly homozygous.We investigated variations in phenotypes,transcription,and histone modifications between two ploidies.Both autotetraploid lines produced larger but fewer tubers than the diploid line.Interestingly,each autotetraploid line displayed ploidy-related differential expression for various genes.We also discovered a genomewide enrichment of H3K27ac in genic regions upon whole-genome doubling(WGD).However,such enrichment was not associated with the differential gene expression between two ploidies.The tetraploid lines may exhibit better resistance to cold-induced sweetening(CIS)than the diploid line in tubers,potentially regulated through the expression of CIS-related key genes,which seems to be associated with the levels of H3K4me3 in cold-stored tubers.These findings will help to understand the impacts of autotetraploidization on dynamics of phenotypes,transcription,and histone modifications,as well as on CIS-related genes in response to cold storage.展开更多
It is revealed in frictional experiments on medium-scale samples that period doubling bifurcation of stress drop for stick-slip occurs due to macroscopic heterogeneity of the sliding surface under conditions for typic...It is revealed in frictional experiments on medium-scale samples that period doubling bifurcation of stress drop for stick-slip occurs due to macroscopic heterogeneity of the sliding surface under conditions for typical stick-slip.The observed data show that the period doubling bifurcation of stress drop results from the alternate occurrence of strain release along the whole fault and along part of fault.This implies that complicated nonlinear behavior corresponds to clear physical implication in some cases.展开更多
Ultrashort pulses complicate the frequency conversion in a nonlinear crystal, where group velocity mismatch becomes the main obstacle due to dispersion. We present a design for group velocity compensated second harmon...Ultrashort pulses complicate the frequency conversion in a nonlinear crystal, where group velocity mismatch becomes the main obstacle due to dispersion. We present a design for group velocity compensated second harmonic generation in a modulated nonlinear structure, embedded in a liquid crystal box. In this structure, nonlinear crystals act as sources of signal and liquid crystals compensate for group velocity mismatch originating from nonlinear crystals. There are the advantages of the flexible, controllable birefringence of liquid crystals. Meanwhile, a method calculating the parameters of this type of structure is presented. To make it clear, an example is provided. Furthermore, the structure can also be shaped as a waveguide to support integration into other optical devices, applicable to all-optical processing systems.展开更多
In 0.1 mol/L HCl medium, 12-tungstophosphoric（TP） acid reacted with matrine（Mat） and oxymatrine（Oxy） to form an ion-association complex. As a result, the new spectra of resonance Rayleigh scattering（RRS）, seco...In 0.1 mol/L HCl medium, 12-tungstophosphoric（TP） acid reacted with matrine（Mat） and oxymatrine（Oxy） to form an ion-association complex. As a result, the new spectra of resonance Rayleigh scattering（RRS）, second-order scattering（SOS） and frequency doubling scattering（FDS） appeared and their intensities were enhanced greatly. The maximum scattering wavelengths of RRS, SOS and FDS were located at 370, 670 and 390 nm, respectively. The in-crements of scattering intensity were directly proportional to the concentration of Mat and Oxy in a certain range. Based on this, the method for the determination of matrine and oxymatrine has been established. It has been applied to the determination of matrine and oxymatrine in samples of Radix sophorae flavescentis with satisfactory result. The reaction mechanism and reasons of RRS enhancement were discussed.展开更多
We report a case of metastatic castration-resistant prostate cancer, who received prior treatment with docetaxel and was then given cabazitaxel as salvage therapy. The patient was monitored by prostate-specific antige...We report a case of metastatic castration-resistant prostate cancer, who received prior treatment with docetaxel and was then given cabazitaxel as salvage therapy. The patient was monitored by prostate-specific antigen doubling time and prostate-specific antigen absolute value. The prostate-specific antigen doubling time was found to be a good response predictor in the patient.展开更多
The factors influencing pollen chromosome doubling in Zenjimaru persimmon were studied, based on the knowledge of meiosis process of pollen mother cells. The results showed: (1) the suitable concentration of colchicin...The factors influencing pollen chromosome doubling in Zenjimaru persimmon were studied, based on the knowledge of meiosis process of pollen mother cells. The results showed: (1) the suitable concentration of colchicine was 0.3-0. 5%; (2) the best doubling effect could be obtained during the period from the diplotene stage to the diakinasis stage, and the highest proportion was up to 40.6%; (3) considering giant pollen ratio and proportion of staminate-flower collection, the better treating frequency was 3 times; (4) giant pollen, which formed mainly from dyad and had bigger cell nucleus volume, was unreduced and hexaploid.展开更多
This paper aims to study the stochastic period-doubling bifurcation of the three-dimensional Rossler system with an arch-like bounded random parameter. First, we transform the stochastic RSssler system into its equiva...This paper aims to study the stochastic period-doubling bifurcation of the three-dimensional Rossler system with an arch-like bounded random parameter. First, we transform the stochastic RSssler system into its equivalent deterministic one in the sense of minimal residual error by the Chebyshev polynomial approximation method. Then, we explore the dynamical behaviour of the stochastic RSssler system through its equivalent deterministic system by numerical simulations. The numerical results show that some stochastic period-doubling bifurcation, akin to the conventional one in the deterministic case, may also appear in the stochastic Rossler system. In addition, we also examine the influence of the random parameter intensity on bifurcation phenomena in the stochastic Rossler system.展开更多
基金supported by the Science and Technology Innovation 2030-Major Project (2023ZD0403001)China Agriculture Research System (CARS-02)+3 种基金Beijing Academy of Agriculture and Forestry Sciences Excellent Scientist Training Program (JKZX202202)National Natural Science Foundation of China (32001554)Beijing Academy of Agriculture and Forestry Sciences Science and Technology Innovation Capability Improvement Project (KJCX20230103)Chinese Universities Scientific Fund (2022TC141).
文摘Doubled haploid(DH)technology is an efficient method used in commercial maize breeding.Chromosome doubling is a vital step of DH technology;however,the underlying processes regulating chromosome doubling of haploid is still not well understood,which is key to optimize the technology.In this study,the immature haploid embryos of the maize inbred line Zheng58 treated with amiprophos-methyl(APM)or colchicine were used to analyze transcriptomic and metabolomic changes,75 and 60 differential expressed metabolites(DEMs)were identified between control treatment,respectively.Most differentially expressed genes(DEGs)related to artificial chromosome doubling were down regulated;these were mainly involved in mitosis process.Both DEMs and DEGs co-expression analyses showed that,compared to controls,zeatin biosynthesis and cofactor and vitamin metabolism were significantly enriched in both APM and colchicine treatments.In a parallel experiment,exogenous vitamins including thiamine,nicotinic acid,vitamin B6,or trans-zeatin were added to colchicine treatment;there were synergistic effects between vitamins or zeatin and colchicine in haploid artificial chromosome doubling.These results provide novel insights in exploring the molecular responses to antimitotic reagents at both the transcriptomic and metabolomic levels.In addition,the application efficiency of haploid breeding will be greatly improved by the key factors for artificial chromosome doubling.
文摘Haploid seedlings were inducted from different maize materials.At 2-3-leaf stage,maize haploids were treated with 0.06% colchicine and 2.0% dimethyl sulfoxide（DMSO） by dipping root,dripping heart leaf and acupuncturing growing point,respectively.The doubling rate and mortality rate in different treatments were analyzed by variance analysis and multiple comparisons.The result showed that growing point acupuncturing method exhibited the highest doubling efficiency with an average doubling rate of 23%,seed-setting rate of 21.4%,and mortality rate of 16.3%.Composed with other two chemical doubling methods,growing point acupuncturing method significantly improved the doubling rate of maize haploids with a lower application dose of colchicine.This study laid the foundation for industrial application of haploid breeding techniques.
文摘Water transport at the root/soil interface of 1 year old Pinus sylvestris Linn. var. sylvestriformis (Takenouchi) Cheng et C. D. Chu seedlings under CO 2 doubling was studied by measuring soil electric conductance to survey soil water profiles and comparing it with root distribution surveyed by soil coring and root harvesting in Changbai Mountain in 1999. The results were: (1) The profiles of soil water content were adjusted by root activity. The water content of the soil layer with abundant roots was higher. (2) When CO 2 concentration was doubled, water transport was more active at the root/soil interface and the roots were distributed into deeper layer. It was shown in this work that the method of measuring electric conductance is an inexpensive, non_destructive and relatively sensitive way for underground water transport process.
基金founded by National Key Technologies R&D Program under Grant No.2007BAC29B03R&D Special Fund for Public WelfareIndustry (meteorology) (GYHY200806010)China-UK-Swiss Adapting to Climate Change in China Project(ACCC)-Climate Science
文摘Potential changes in precipitation extremes in July–August over China in response to CO 2 doubling are analyzed based on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （20C3M） experiment and the 1% per year CO 2 increase experiment （to doubling） （1pctto2x） of phase 3 of the Coupled Model Inter-comparison Project （CMIP3）. Evaluation of the models’ performance in simulating the mean state shows that the majority of models fairly reproduce the broad spatial pattern of observed precipitation. However, all the models underestimate extreme precipitation by ～50%. The spread among the models over the Tibetan Plateau is ～2–3 times larger than that over the other areas. Models with higher resolution generally perform better than those with lower resolutions in terms of spatial pattern and precipitation amount. Under the 1pctto2x scenario, the ratio between the absolute value of MME extreme precipitation change and model spread is larger than that of total precipitation, indicating a relatively robust change of extremes. The change of extreme precipitation is more homogeneous than the total precipitation. Analysis on the output of Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 （GFDL-CM2.1） indicates that the spatially consistent increase of surface temperature and water vapor content contribute to the large increase of extreme precipitation over contiguous China, which follows the Clausius–Clapeyron relationship. Whereas, the meridionally tri-polar pattern of mean precipitation change over eastern China is dominated by the change of water vapor convergence, which is determined by the response of monsoon circulation to global warming.
基金supported by R&D Special Fund for Public Welfare Industry (meteorology) (GYHY200806010)China–UK–Swiss Adapting to Climate Change in China Project (ACCC)–Climate Sciencethe National Key Technologies R&D Program under Grant No. 2007BAC29B03
文摘This is the second part of the authors’ analysis on the output of 24 coupled climate models from the Twentieth-Century Climate in Coupled Models （20C3M） experiment and 1% per year CO 2 increase experiment （to doubling） （1pctto2x） of phase 3 of the Coupled Model Inter-comparison Project （CMIP3）. The study focuses on the potential changes of July–August temperature extremes over China. The pattern correlation coefficients of the simulated temperature with the observations are 0.6–0.9, which are higher than the results for precipitation. However, most models have cold bias compared to observation, with a larger cold bias over western China （5°C） than over eastern China （2°C）. The multi-model ensemble （MME） exhibits a significant increase of temperature under the 1pctto2x scenario. The amplitude of the MME warming shows a northwest–southeast decreasing gradient. The warming spread among the models （～1°C– 2°C） is less than MME warming （～2°C–4°C）, indicating a relatively robust temperature change under CO 2 doubling. Further analysis of Geophysical Fluid Dynamics Laboratory coupled climate model version 2.1 （GFDL-CM2.1） simulations suggests that the warming pattern may be related to heat transport by summer monsoons. The contrast of cloud effects also has contributions. The different vertical structures of warming over northwestern China and southeastern China may be attributed to the different natures of vertical circulations. The deep, moist convection over southeastern China is an effective mechanism for ＂transporting＂ the warming upward, leading to more upper-level warming. In northwestern China, the warming is more surface-orientated, possibly due to the shallow, dry convection.
文摘AIM To evaluate the efficacy of doubling time(DT) of gastrointestinal submucosal tumors(GIST).METHODS From April 1987 through November 2012, a total of 323 patients were given a final histopathological diagnosis of GISTs on surgical resection or endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA) in Kitasato University East Hospital or Kitasato University Hospital. We studied 53 of these patients(34 with resected tumors and 19 with unresected tumors) whose tumors could be measured on EUS on at least two successive occasions. The histopathological diagnosis was GIST in 34 patients, leiomyoma in 5, schwannoma in 3, ectopic pancreas in 1, hamartoma in 1, cyst in 1, Brunner's adenoma in 1, and spindle-cell tumor in 7. We retrospectively calculated the DT of GISTs on the basis of the time course of EUS findings to estimate the growth rate of such tumors.RESULTS The DT was 17.2 mo for GIST, as compared with 231.2 mo for leiomyoma, 104.7 mo for schwannoma, 274.9mo for ectopic pancreas, 61.2 mo for hamartoma, 49.0 mo for cyst, and 134.7 mo for Brunner's adenoma. The GISTs were divided into risk classes on the basis of tumor diameters and mitotic figures(Fletcher's classification). The classification was extremely low risk or low risk in 28 patients, intermediate risk in 3, and high risk in 3. DT of GIST according to risk was 24.0 mo for extremely low-risk plus low-risk GIST, 17.1 mo for intermediate-risk GIST, and 3.9 mo for high-risk GIST. DT of GIST was significantly shorter than that of leiomyoma plus schwannoma(P < 0.05), and DT of high-risk GIST was significantly shorter than that of extremely low-risk plus low-risk GIST(P < 0.05).CONCLUSION For GIST, a higher risk grade was associated with a significantly shorter DT. Small SMTs should initially be followed up within 6 mo after detection.
基金supported by the NSFC 40830106 and 40975038Innovation Group Project 40921004Ministry of Science and Technology of China(National Key Program for Developing Basic Science 2007CB411803 and 2010CB428904)
文摘Based on simulations of the IPCC 20C3M and SRES A1B experiments in ten coupled models, the Asian summer mon-soon (ASM) response to CO2 doubling and the different responses among models are examined. Nine models show the similar results that the weakening of land-ocean thermal contrast caused by the CO2 doubling contributes to a weaker large-scale ASM circulation. Further analysis in this study also shows that the major ASM components,such as the Somali cross-equatorial flow,the low level India-South China Sea monsoon trough,and the upper level tropical easterly jet stream, weaken as CO2 doubles.However,the ASM rainfall increases as a result of the increased moisture from the warmer Indian Ocean and the South China Sea, and the enhanced northward moisture transport over the ASM region.For the response of enhanced northward moisture transport over South Asia, the positive contribution of moisture content increase in the Indian Ocean is dominant and the negative contribution of the weaker mon-soon circulation is secondary at 850 hPa,but both have positive contribution to the total moisture transport along the East China coast.The paradox of the weaker ASM circulation and the increasing precipitation in CO2 doubling is confirmed.It is found that strengthening of northward moisture transport could intensify the precipitation and atmospheric heat source over the north Arabian Sea and East China,and result in enhanced southwesterly at 850 hPa as global warming occurs.All ten models show significant enhanced southwesterly response over the north Arabian Sea,and six of them show enhanced southwesterly response along the East China coast.
基金This work was supported by the National Natural Science Foundation of China(No.81770168 and No.81570110).
文摘Objective:Although the effect of decitabine on myelodysplastic syndrome(MDS)has been demonstrated,merely a proportion of patients respond to therapy,and no well-recognized predictors have been identified.This study was conducted to investigate the effectiveness of decitabine in real-world clinical practice,and determine the predictive factors of response and overall survival(OS)in MDS patients.
文摘Studies of barley and maize indicate that chromosome doubling occurs via nuclear fusion during an early stage of microspore embryogenesis, but the time and mechanism by which chromosome doubling occurs in bread wheat (Triticum aestivum) remains undetermined. The purpose of this study was to determine the relative time during induction culture when chromosome doubling may occur in wheat, and to identify early indicators for doubled haploid microspores. Microspore nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) and observed under a fluorescent microscope on the day of isolation, three days after isolation, and six days after isolation. The change in the percentage of microspores containing a single small nucleus, two small nuclei, a single enlarged nucleus, and three or more nuclei was then tracked throughout the six-day period. Ploidy levels were estimated by determining the cross-sectional area and number of nucleoli in microspores containing small and large nuclei then comparing the results of each respective cell-type. The percentage of microspores containing enlarged nuclei increased throughout the six-day test period, and the percentage of binucleated microspores containing small nuclei decreased. Comparison of the changes in average percentage of microspores containing a single small nucleus, binucleated microspores, microspores containing a single large nucleus, and multinucleate microspores on days 0, 3, and 6 indicates that nuclei classified as “small” are likely haploids and nuclei classified as “large” are doubled haploids. The percentage of microspores with enlarged nucleus (nuclei) during the first six days of induction culture could be used as an early indicator for the frequency of chromosome doubling in wheat microspore culture.
文摘An improved recursive doubling algorithm for solving linear recurrence R <n,1>is given,whose parallel time complexity is (τ++τ.) logn when n processors are available,achieving the lower bound in array processor type computation.
基金supported by grants from the National Natural Science Foundation of China(31900386 to Z.Z.)Sichuan Science and Technology Program(2021YFH0025 to Z.Z.and 2021YFYZ0019 to B.Z.and Z.Z.)+1 种基金State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China at Sichuan Agricultural University(SKL-KF202205 to B.Z.)State Key Laboratory of Crop Biology Open Fund(2020KF01 to B.Z.)。
文摘Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts.Extensive studies have shown genetic,transcriptomic,and epigenetic dynamics upon polyploidization in multiple plant species.However,few studies have unveiled those alternations imposed only by ploidy level,without any interference from heterozygosity.Cultivated potato is highly heterozygous.Thus,in this study,we developed two homozygous autotetraploid lines and one homozygous diploid line in parallel from a homozygous diploid potato.We confirmed their ploidy levels using chloroplast counting and karyotyping.Oligo-FISH and genome re-sequencing validated that these potato lines are nearly homozygous.We investigated variations in phenotypes,transcription,and histone modifications between two ploidies.Both autotetraploid lines produced larger but fewer tubers than the diploid line.Interestingly,each autotetraploid line displayed ploidy-related differential expression for various genes.We also discovered a genomewide enrichment of H3K27ac in genic regions upon whole-genome doubling(WGD).However,such enrichment was not associated with the differential gene expression between two ploidies.The tetraploid lines may exhibit better resistance to cold-induced sweetening(CIS)than the diploid line in tubers,potentially regulated through the expression of CIS-related key genes,which seems to be associated with the levels of H3K4me3 in cold-stored tubers.These findings will help to understand the impacts of autotetraploidization on dynamics of phenotypes,transcription,and histone modifications,as well as on CIS-related genes in response to cold storage.
文摘It is revealed in frictional experiments on medium-scale samples that period doubling bifurcation of stress drop for stick-slip occurs due to macroscopic heterogeneity of the sliding surface under conditions for typical stick-slip.The observed data show that the period doubling bifurcation of stress drop results from the alternate occurrence of strain release along the whole fault and along part of fault.This implies that complicated nonlinear behavior corresponds to clear physical implication in some cases.
基金Supported by the Natural Science Foundation of Heilongjiang Province under Grant Nos F201312,F2016023 and QC2015086the National Natural Science Foundation of China under Grant No 61405049
文摘Ultrashort pulses complicate the frequency conversion in a nonlinear crystal, where group velocity mismatch becomes the main obstacle due to dispersion. We present a design for group velocity compensated second harmonic generation in a modulated nonlinear structure, embedded in a liquid crystal box. In this structure, nonlinear crystals act as sources of signal and liquid crystals compensate for group velocity mismatch originating from nonlinear crystals. There are the advantages of the flexible, controllable birefringence of liquid crystals. Meanwhile, a method calculating the parameters of this type of structure is presented. To make it clear, an example is provided. Furthermore, the structure can also be shaped as a waveguide to support integration into other optical devices, applicable to all-optical processing systems.
基金Supported by the National Natural Science Foundation of China(No.20875078)
文摘In 0.1 mol/L HCl medium, 12-tungstophosphoric（TP） acid reacted with matrine（Mat） and oxymatrine（Oxy） to form an ion-association complex. As a result, the new spectra of resonance Rayleigh scattering（RRS）, second-order scattering（SOS） and frequency doubling scattering（FDS） appeared and their intensities were enhanced greatly. The maximum scattering wavelengths of RRS, SOS and FDS were located at 370, 670 and 390 nm, respectively. The in-crements of scattering intensity were directly proportional to the concentration of Mat and Oxy in a certain range. Based on this, the method for the determination of matrine and oxymatrine has been established. It has been applied to the determination of matrine and oxymatrine in samples of Radix sophorae flavescentis with satisfactory result. The reaction mechanism and reasons of RRS enhancement were discussed.
文摘We report a case of metastatic castration-resistant prostate cancer, who received prior treatment with docetaxel and was then given cabazitaxel as salvage therapy. The patient was monitored by prostate-specific antigen doubling time and prostate-specific antigen absolute value. The prostate-specific antigen doubling time was found to be a good response predictor in the patient.
基金supported by the National Natural Science Foundation of China(30070529)
文摘The factors influencing pollen chromosome doubling in Zenjimaru persimmon were studied, based on the knowledge of meiosis process of pollen mother cells. The results showed: (1) the suitable concentration of colchicine was 0.3-0. 5%; (2) the best doubling effect could be obtained during the period from the diplotene stage to the diakinasis stage, and the highest proportion was up to 40.6%; (3) considering giant pollen ratio and proportion of staminate-flower collection, the better treating frequency was 3 times; (4) giant pollen, which formed mainly from dyad and had bigger cell nucleus volume, was unreduced and hexaploid.
基金Project supported by the National Natural Science Foundation of China (Grant No. 10872165)
文摘This paper aims to study the stochastic period-doubling bifurcation of the three-dimensional Rossler system with an arch-like bounded random parameter. First, we transform the stochastic RSssler system into its equivalent deterministic one in the sense of minimal residual error by the Chebyshev polynomial approximation method. Then, we explore the dynamical behaviour of the stochastic RSssler system through its equivalent deterministic system by numerical simulations. The numerical results show that some stochastic period-doubling bifurcation, akin to the conventional one in the deterministic case, may also appear in the stochastic Rossler system. In addition, we also examine the influence of the random parameter intensity on bifurcation phenomena in the stochastic Rossler system.
