DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and p...DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and potential functions,are poorly understood in wheat.Here,we identified 198985 DSCNVs by investigating 491 genomes of common wheat and found that they account for 20%of the entire genome.Interestingly,approximately 38%of genes are linked to DSCNVs.The number of DSCNVs within each accession ranges from 47366 to 96342,and their total sizes vary from 421.3 to 1267.9 Mb.We found that 957 and 1304 DSCNVs have been favored by breeders in China and the United States,respectively.By conducting DSCNV-based genome-wide association studies for the principal components of plant developmental and yield-component traits,we identified 34 loci as directly or indirectly involved in controlling the formation of multiple traits.Notably,a newly discovered DSCNV covering TaFT-D1 is significantly associated with flowering time and other agronomic traits.Overall,our findings highlight the potential of DSCNVs to drive fundamental discoveries in plant science.The comprehensive DSCNV map and the DSCNV-associated genes will also facilitate future research efforts to improve wheat yield,quality,and adaptation.展开更多
研究了基因序列分析中的DNA序列相似性重复片段的查找问题.在对重复片段的相似性衡量进行分析之后,基于海明距离提出了新的相似度衡量标准模式相似度和片段相似度,并在此基础上提出了一个新的相似性重复片段的定义SATR(segment-similari...研究了基因序列分析中的DNA序列相似性重复片段的查找问题.在对重复片段的相似性衡量进行分析之后,基于海明距离提出了新的相似度衡量标准模式相似度和片段相似度,并在此基础上提出了一个新的相似性重复片段的定义SATR(segment-similarity based approximate tandem repeats).在进行SATR的查找时,采用了一个轻量级的索引后继数组,并设计出在后继数组上进行SATR查找的算法.实验评估和性能分析表明,基于后继数组的SATR查找算法在查找结果和查找时间上都要优于其他同类方法.展开更多
本文对植物外源总 DNA 导入技术在国内外的研究进展进行了综述,提供了这一技术成功地在棉花、水稻、高梁、小麦、大豆、烟草、玉米等农作物上应用的实例,分析外源总 DNA 导入技术与植物基因工程这二个不同层次的农业分子育种的研究现状...本文对植物外源总 DNA 导入技术在国内外的研究进展进行了综述,提供了这一技术成功地在棉花、水稻、高梁、小麦、大豆、烟草、玉米等农作物上应用的实例,分析外源总 DNA 导入技术与植物基因工程这二个不同层次的农业分子育种的研究现状和进展。作者认为:外源总 DNA 导入技术简便实用,可将 DNA 直接导入栽培植物,培育出优质、高产、抗病虫害、抗逆的作物新品种,产生巨大的经济效益。展开更多
基金supported by the National Natural Science Foundation of China(grant nos.31991211,31921005,and 32201745)the Strategic Pri-ority Research Program of the Chinese Academy of Sciences(XDA24010104)+1 种基金the Hainan Seed Industry Laboratory(B23C1000402)the Major Basic Research Program of Shandong Natural Science Foundation(ZR2019ZD15).
文摘DNA-segment copy number variations(DSCNVs),such as deletions and duplications,are important sources of genomic structural variation.However,the types and sizes of DSCNVs,as well as their genome-wide distribution and potential functions,are poorly understood in wheat.Here,we identified 198985 DSCNVs by investigating 491 genomes of common wheat and found that they account for 20%of the entire genome.Interestingly,approximately 38%of genes are linked to DSCNVs.The number of DSCNVs within each accession ranges from 47366 to 96342,and their total sizes vary from 421.3 to 1267.9 Mb.We found that 957 and 1304 DSCNVs have been favored by breeders in China and the United States,respectively.By conducting DSCNV-based genome-wide association studies for the principal components of plant developmental and yield-component traits,we identified 34 loci as directly or indirectly involved in controlling the formation of multiple traits.Notably,a newly discovered DSCNV covering TaFT-D1 is significantly associated with flowering time and other agronomic traits.Overall,our findings highlight the potential of DSCNVs to drive fundamental discoveries in plant science.The comprehensive DSCNV map and the DSCNV-associated genes will also facilitate future research efforts to improve wheat yield,quality,and adaptation.
文摘研究了基因序列分析中的DNA序列相似性重复片段的查找问题.在对重复片段的相似性衡量进行分析之后,基于海明距离提出了新的相似度衡量标准模式相似度和片段相似度,并在此基础上提出了一个新的相似性重复片段的定义SATR(segment-similarity based approximate tandem repeats).在进行SATR的查找时,采用了一个轻量级的索引后继数组,并设计出在后继数组上进行SATR查找的算法.实验评估和性能分析表明,基于后继数组的SATR查找算法在查找结果和查找时间上都要优于其他同类方法.
文摘本文对植物外源总 DNA 导入技术在国内外的研究进展进行了综述,提供了这一技术成功地在棉花、水稻、高梁、小麦、大豆、烟草、玉米等农作物上应用的实例,分析外源总 DNA 导入技术与植物基因工程这二个不同层次的农业分子育种的研究现状和进展。作者认为:外源总 DNA 导入技术简便实用,可将 DNA 直接导入栽培植物,培育出优质、高产、抗病虫害、抗逆的作物新品种,产生巨大的经济效益。
