In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and ...In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and summarized in this paper,such as RAPD,ISSR,RFLP,AFLP,SNP,and DNA bar code sequence analysis techniques.By consulting Sciencedirect databases and Web of Science databases,2348 related articles were found,of which 39 were related to molecular identification techniques and traditional Chinese medicine.The application of the molecular identification techniques in four aspects was reviewed,namely the identification on the authenticity(true or false),multi-source identification and genetic diversity,producing area,and growing year discrimination of traditional Chinese medicine.展开更多
To further improvc the application of DNA fingerprinting technique in adulterated food identification and traceability, the paper briefly introduced the ap- plication of common DNA fingerprinting techniques, such as s...To further improvc the application of DNA fingerprinting technique in adulterated food identification and traceability, the paper briefly introduced the ap- plication of common DNA fingerprinting techniques, such as species-specific PCR, RAPD, AFLP, ISSR, SSR and SNP in adulterated food identification and traceability.展开更多
Epigenetics is the study of phenotypic variations that do not alter DNA sequences.Cancer epigenetics has grown rapidly over the past few years as epigenetic alterations exist in all human cancers.One of these alterati...Epigenetics is the study of phenotypic variations that do not alter DNA sequences.Cancer epigenetics has grown rapidly over the past few years as epigenetic alterations exist in all human cancers.One of these alterations is DNA methylation;an epigenetic process that regulates gene expression and often occurs at tumor suppressor gene loci in cancer.Therefore,studying this methylation process may shed light on different gene functions that cannot otherwise be interpreted using the changes that occur in DNA sequences.Currently,microarray technologies;such as Illumina Infinium BeadChip assays;are used to study DNA methylation at an extremely large number of varying loci.At each DNA methylation site,a beta value(β)is used to reflect the methylation intensity.Therefore,clustering this data from various types of cancers may lead to the discovery of large partitions that can help objectively classify different types of cancers aswell as identify the relevant loci without user bias.This study proposed a Nested Big Data Clustering Genetic Algorithm(NBDC-GA);a novel evolutionary metaheuristic technique that can perform cluster-based feature selection based on the DNA methylation sites.The efficacy of the NBDC-GA was tested using real-world data sets retrieved from The Cancer Genome Atlas(TCGA);a cancer genomics program created by the NationalCancer Institute(NCI)and the NationalHuman Genome Research Institute.The performance of the NBDC-GA was then compared with that of a recently developed metaheuristic Immuno-Genetic Algorithm(IGA)that was tested using the same data sets.The NBDC-GA outperformed the IGA in terms of convergence performance.Furthermore,the NBDC-GA produced a more robust clustering configuration while simultaneously decreasing the dimensionality of features to a maximumof 67%and of 94.5%for individual cancer type and collective cancer,respectively.The proposed NBDC-GA was also able to identify two chromosomes with highly contrastingDNAmethylations activities that were previously linked to cancer.展开更多
宫腔内人工授精(intrauterine insemination,IUI)因其操作简便、成本效益高而成为一线辅助生殖方案,但其成功率多停滞在10%~15%。精子DNA碎片(sperm DNA fragmentation,SDF)是评估精子功能的重要指标,其形成机制涉及内源性因素(如氧化...宫腔内人工授精(intrauterine insemination,IUI)因其操作简便、成本效益高而成为一线辅助生殖方案,但其成功率多停滞在10%~15%。精子DNA碎片(sperm DNA fragmentation,SDF)是评估精子功能的重要指标,其形成机制涉及内源性因素(如氧化应激、凋亡异常和染色质重塑异常)和外源性因素(如环境、生活方式及医源性影响)。常用的几种SDF检测方法因其临界值不统一,给检测结果的解读带来了挑战。SDF升高对IUI结局具有显著影响,包括降低受精率、妊娠率,并增加不良妊娠结局风险。目前针对高SDF可采取的干预策略有生活方式调整、药物或手术治疗,以及合理选择辅助生殖技术。临床上建议对反复IUI失败或不明原因不孕的夫妇进行SDF检测,并采取个体化综合治疗策略,以改善生育结局。展开更多
基金supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and summarized in this paper,such as RAPD,ISSR,RFLP,AFLP,SNP,and DNA bar code sequence analysis techniques.By consulting Sciencedirect databases and Web of Science databases,2348 related articles were found,of which 39 were related to molecular identification techniques and traditional Chinese medicine.The application of the molecular identification techniques in four aspects was reviewed,namely the identification on the authenticity(true or false),multi-source identification and genetic diversity,producing area,and growing year discrimination of traditional Chinese medicine.
基金Supported by the Youth Foundation of Sichuan Academy of Agricultural Sciences(2009QNJJ-037,2010QNJJ-031)the Monitoring on Alien Biological Invasion(the Project of Ministry of Agriculture)
文摘To further improvc the application of DNA fingerprinting technique in adulterated food identification and traceability, the paper briefly introduced the ap- plication of common DNA fingerprinting techniques, such as species-specific PCR, RAPD, AFLP, ISSR, SSR and SNP in adulterated food identification and traceability.
文摘Epigenetics is the study of phenotypic variations that do not alter DNA sequences.Cancer epigenetics has grown rapidly over the past few years as epigenetic alterations exist in all human cancers.One of these alterations is DNA methylation;an epigenetic process that regulates gene expression and often occurs at tumor suppressor gene loci in cancer.Therefore,studying this methylation process may shed light on different gene functions that cannot otherwise be interpreted using the changes that occur in DNA sequences.Currently,microarray technologies;such as Illumina Infinium BeadChip assays;are used to study DNA methylation at an extremely large number of varying loci.At each DNA methylation site,a beta value(β)is used to reflect the methylation intensity.Therefore,clustering this data from various types of cancers may lead to the discovery of large partitions that can help objectively classify different types of cancers aswell as identify the relevant loci without user bias.This study proposed a Nested Big Data Clustering Genetic Algorithm(NBDC-GA);a novel evolutionary metaheuristic technique that can perform cluster-based feature selection based on the DNA methylation sites.The efficacy of the NBDC-GA was tested using real-world data sets retrieved from The Cancer Genome Atlas(TCGA);a cancer genomics program created by the NationalCancer Institute(NCI)and the NationalHuman Genome Research Institute.The performance of the NBDC-GA was then compared with that of a recently developed metaheuristic Immuno-Genetic Algorithm(IGA)that was tested using the same data sets.The NBDC-GA outperformed the IGA in terms of convergence performance.Furthermore,the NBDC-GA produced a more robust clustering configuration while simultaneously decreasing the dimensionality of features to a maximumof 67%and of 94.5%for individual cancer type and collective cancer,respectively.The proposed NBDC-GA was also able to identify two chromosomes with highly contrastingDNAmethylations activities that were previously linked to cancer.
文摘宫腔内人工授精(intrauterine insemination,IUI)因其操作简便、成本效益高而成为一线辅助生殖方案,但其成功率多停滞在10%~15%。精子DNA碎片(sperm DNA fragmentation,SDF)是评估精子功能的重要指标,其形成机制涉及内源性因素(如氧化应激、凋亡异常和染色质重塑异常)和外源性因素(如环境、生活方式及医源性影响)。常用的几种SDF检测方法因其临界值不统一,给检测结果的解读带来了挑战。SDF升高对IUI结局具有显著影响,包括降低受精率、妊娠率,并增加不良妊娠结局风险。目前针对高SDF可采取的干预策略有生活方式调整、药物或手术治疗,以及合理选择辅助生殖技术。临床上建议对反复IUI失败或不明原因不孕的夫妇进行SDF检测,并采取个体化综合治疗策略,以改善生育结局。
