In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and ...In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and summarized in this paper,such as RAPD,ISSR,RFLP,AFLP,SNP,and DNA bar code sequence analysis techniques.By consulting Sciencedirect databases and Web of Science databases,2348 related articles were found,of which 39 were related to molecular identification techniques and traditional Chinese medicine.The application of the molecular identification techniques in four aspects was reviewed,namely the identification on the authenticity(true or false),multi-source identification and genetic diversity,producing area,and growing year discrimination of traditional Chinese medicine.展开更多
美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的...美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的序列进行PCR扩增和测序,将所得序列用于蓟马种类的分子鉴定。使用不同的矩阵和系统发育构建方法对待鉴定的蓟马以及另外38种蓟马进行了聚类分析,结果表明:基于线粒体COⅠ基因第1和2编码位点,利用NJ法构建的系统发育树可以较好地区别不同蓟马种类,可作为调查我国蓟马种类和分布的快速方便方法。最后对美洲棘蓟马的基本生物学特性和防控对策进行了综述。展开更多
基金supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘In recent years,molecular identification technology has become the predominant approach for the identification of traditional Chinese medicine.The molecular identification techniques in recent years were analyzed and summarized in this paper,such as RAPD,ISSR,RFLP,AFLP,SNP,and DNA bar code sequence analysis techniques.By consulting Sciencedirect databases and Web of Science databases,2348 related articles were found,of which 39 were related to molecular identification techniques and traditional Chinese medicine.The application of the molecular identification techniques in four aspects was reviewed,namely the identification on the authenticity(true or false),multi-source identification and genetic diversity,producing area,and growing year discrimination of traditional Chinese medicine.
文摘美洲棘蓟马Echinothrips americanus Morgan,1913是新入侵我国的种类。本文报道了该虫各发育阶段的外部形态和分子鉴定结果。首先利用体视显微镜对美洲棘蓟马不同虫态的外部形态进行了观察和拍照;再选择线粒体COⅠ基因中一段约430bp的序列进行PCR扩增和测序,将所得序列用于蓟马种类的分子鉴定。使用不同的矩阵和系统发育构建方法对待鉴定的蓟马以及另外38种蓟马进行了聚类分析,结果表明:基于线粒体COⅠ基因第1和2编码位点,利用NJ法构建的系统发育树可以较好地区别不同蓟马种类,可作为调查我国蓟马种类和分布的快速方便方法。最后对美洲棘蓟马的基本生物学特性和防控对策进行了综述。
