Circular double stranded DNA has different topological states which are defined by their linking numbers.Equilibrium distribution of linking numbers can be obtained by closing a linear DNA into a circle by ligase.Usin...Circular double stranded DNA has different topological states which are defined by their linking numbers.Equilibrium distribution of linking numbers can be obtained by closing a linear DNA into a circle by ligase.Using Monte Carlo simulation,we predict the temperature dependence of the linking number distribution of small circular DNAs.Our predictions are based on flexible defect excitations resulted from local melting or unstacking of DNA base pairs.We found that the reduced bending rigidity alone can lead to measurable changes of the variance of linking number distribution of short circular DNAs.展开更多
基金supported by the Young Investigator Award received by Yan in 2006the Foundation for the Visiting PhD Candidate of the Chinese Academy of Science received by Liu in 2006
文摘Circular double stranded DNA has different topological states which are defined by their linking numbers.Equilibrium distribution of linking numbers can be obtained by closing a linear DNA into a circle by ligase.Using Monte Carlo simulation,we predict the temperature dependence of the linking number distribution of small circular DNAs.Our predictions are based on flexible defect excitations resulted from local melting or unstacking of DNA base pairs.We found that the reduced bending rigidity alone can lead to measurable changes of the variance of linking number distribution of short circular DNAs.
